• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.88-88
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• 2018

In order to increase the germination speed and uniformity of tomato seeds, sonication and modified drum priming treatments were investigated to produce high quality seeds for export. Sonication treatment was performed for 5, 10 and 20minutes at an intensity of 5.2, 10.4 and 15.7kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $KNO_3$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 60, 72 and 84h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. Germination speed were highly improved by sonication with osmotic priming. The seed treatment of osmotic priming or hydro priming after sonication or sonication without priming enhanced germination percentage (GP) on the $2^{rd}$day after sowing to 46%, 43% and 28%, respectively, while untreated seeds resulted in only 1% GP. These treatments also highly improved mean germination time (MGT) to 1.4, 1.8 and 2.6days, respectively, when compared to 3.5days MGT of untreated seeds. The modified drum priming treatment (72h incubation after 60% SMC hydration) significantly improved results of 74% GP(on the $3^{rd}$day after sowing), 2.6days MGT and $39%{\cdot}day^{-1}$ germination rate (GR), however, untreated seeds showed 19% GP, 4.1 MGT, and $25%{\cdot}day^{-1}$ GR. Although osmotic priming after sonication, hydro priming showed similar improved germination characteristics, however, modified drum priming is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.87-87
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• 2018

This study was conducted to investigate the effects of osmotic priming, liquid smoke (LS), sonication and modified drum priming treatments to improve the germination speed and uniformity of pepper seeds for high quality export seeds. Seeds were treated in 0.5, 1.0, 5.0, 10% LS solution only or with 100mM $K_2SO_4$ solution for 6days at $15^{\circ}C$. Sonication treatment was performed for 5, 10, and 20 minutes at an intensity of 5.2, 10.4, 15.7, 21.0, 26.1kHz in water at $15^{\circ}C$. After sonication treatment, seeds were primed with water or 100mM $K_2SO_4$ for 4days. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 84, 96 and 108h in a container with a relative humidity of 99% at 26rpm for a modified drum priming treatments. 0.5% LS treatment with water or 100mM $K_2SO_4$ priming showed significant effects with 71% GP (9.0days MGT) and 66% GP (7.4days MGT), respective, while untreated seeds resulted in only 61% GP and 10days MGT. The healthy seed % was increased by 24% by $K_2SO_4$ priming with LS treatment compared to untreated seeds. However, sonication treatment showed less germination or no difference compared to untreated seed, regardless of intensity and treatment time. The modified drum priming treatment (108h incubation after 50% SMC hydration) significantly improved the GP (on the $4^{th}$days after sowing), germination rate (GR), and MGT to 65%, $28%{\cdot}day^{-1}$, and 3.6days, whereas untreated seed resulted only 7% GP, $17%{\cdot}day^{-1}$ GR and 5.9days MGT. Thus, modified drum priming treatment effectively improved seed germination and is considered as an industrially promising treatment methods considering the shortening of the treatment period and environment-friendly aspects.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.86-86
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• 2018

This study was conducted to investigate the effects of modified drum priming and 24-Epibrassinolide (24-EBL) treatment to improve the seed quality for export. 40, 50 and 60% seed moisture content (SMC) of hydrated seeds were incubated for 16 and 24 h in a container with a relative humidity of 99% at 26 rpm for a modified drum priming treatment. The treated seeds were sown at $20^{\circ}C$ and $30^{\circ}C$ (12/12h, light/dark) with four replications of 25 seeds on pleated paper. The seeds were hydrated with water or 24-EBL solutions of $10^{-7}$, $10^{-8}$ and $10^{-9}M$, respectively. The germination of the modified drum primed seeds (24 h incubation after 60% SMC hydration) improved to 1.6 days mean germination time (MGT) and $46%{\cdot}day^{-1}$ germination rate (GR), while the untreated seeds showed 2.1 days MGT and $28%{\cdot}day^{-1}$ GR. The modified drum priming (60% SMC and 24 h incubation with $10^{-9}M$ 24-EBL) showed improved results in MGT (1.8 days) and GR (55%) at $20^{\circ}C$, whereas untreated seeds showed 2.3 days MGT and 44% GR. Under $30^{\circ}C$, germination of modified drum primed seeds was significantly improved in GP (80%), GR ($31%{\cdot}day^{-1}$), HS (55%) and MGT (3.3 days), however, untreated seeds showed decreased GP (27%), GR ($22%{\cdot}day^{-1}$), HS (55%) and MGT (4.8 days). This study showed that the germination of lettuce seeds is enhanced by 24 h drum incubation with 24-EBL and this method can be used effectively to achieve the benefits of early germination and uniform seedling development. In addition, these treatments circumvent thermo-dormancy of lettuce seed and have a possibility of high-quality and environment-friendly seed processing.