• Title/Summary/Keyword: Drosophila robusta group

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Drosophila robusta species group 2종 (D.lacertosa 와 D.sordidula)의 mtDNA 변이에 의한 종분화정도

  • 최준길;박제철
    • Animal Systematics, Evolution and Diversity
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    • v.11 no.4
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    • pp.469-477
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    • 1995
  • Drosophila lacertosa and D. sordidula are members of the robusta species group in virilis section of Drosophila. The mtDNA of both species was analyzed, using 10 restriction endonucleases. The mtDNA genome size of D. lacertosa and D. sordidula was 15.7 kbp, altogether, and the numbers of mtDNA fragment were 26 and 32, respectively. Restriction cleavage map of mtDNA in these species was constructed. The patterns of cleavage map were very different between two species and it means that speciation was taken for a long time ago.

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Isolation and Partial Chemical Characterization of the Yolk Proteins from Drosophila sp. (robusta species group) (Drosophila sp.(robusta species group)의 난황 단백질의 분리 및 부분적 화학적 특성)

  • Kim, Se-Jae;Gi
    • The Korean Journal of Zoology
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    • v.35 no.1
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    • pp.17-22
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    • 1992
  • The three yolk polypeptides have been isolated and partially characterized. Their molecular weights of YPI, YP2, and YP3 were 48, 000, 47, 000, and 46, 000, respectivelv, as judged by SDS-polyacrvlamide gel electrophoresis. They have different digestion products upon in situ peptide mapping by limited proteolysis. Two-dimensional gel electrophoresis showed that their isoelectric points were heterogeneous from 5.92 to 6.54. And thew showed three different antigen-antibody reactions when each polvpeptides is reacted with antisera made to a mixture of all of three. These data reported here indicate that the yolk proteins are consisted of distinctive polypeptides in Drosophlla sp. (robusta species group).

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Effect of 20-Hydroxyecdysone on Yolk Protein Synthesis in Drosophila sp. (robusta Species Group) (제주먹초파리에서 난황 단백질 합성에 미치는 20-Hydroxyecdysone의 영향)

  • 김세재;김기옥오문유이정주
    • The Korean Journal of Zoology
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    • v.35 no.3
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    • pp.271-276
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    • 1992
  • 20-hydroxvecdysone (20-HE) seems to be related in the regulation of vitellogenesis in Drosophifa sp. (robwta species sroup). Although yolk proteins (YPs) synthesis does not occur at a high rate in fat body cells of one daw-old female after eclosion, application of 20-HE to isolated abdomens deprived of anterior endocrine glands stimulated the synthesis and secretion of YPs into the hemolvmph. An injection of 0.3 $\mul$ of a 10-s M 20-HE was sufficient topromote synthesis and secretion of YPs in isolated abdomens. The response of isolated automens to hormones was first detected between 2 hr and 3 hr after treaDent of 10-s M 20-HE. Transcript analysis showed that the effect of 20-HE on yolk protein synthesis was mediated at the level of transcription.

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