• Journal of Ginseng Research
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• 제42권4호
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• pp.512-523
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• 2018

Background: 20(S)-Protopanaxadiol (20S-PPD) is a fully deglycosylated ginsenoside metabolite and has potent dermal antiaging activity. However, because of its low aqueous solubility and large molecular size, a suitable formulation strategy is required to improve its solubility and skin permeability, thereby enhancing its skin deposition. Thus, we optimized microemulsion (ME)-based hydrogel (MEH) formulations for the topical delivery of 20S-PPD. Methods: MEs and MEHs were formulated and evaluated for their particle size distribution, morphology, drug loading capacity, and stability. Then, the deposition profiles of the selected 20S-PPD-loaded MEH formulation were studied using a hairless mouse skin model and Strat-M membrane as an artificial skin model. Results: A Carbopol-based MEH system of 20S-PPD was successfully prepared with a mean droplet size of 110 nm and narrow size distribution. The formulation was stable for 56 d, and its viscosity was high enough for its topical application. It significantly enhanced the in vitro and in vivo skin deposition of 20S-PPD with no influence on its systemic absorption in hairless mice. Notably, it was found that the Strat-M membrane provided skin deposition data well correlated to those obtained from the in vitro and in vivo mouse skin studies on 20S-PPD (correlation coefficient $r^2=0.929-0.947$). Conclusion: The MEH formulation developed in this study could serve as an effective topical delivery system for poorly soluble ginsenosides and their deglycosylated metabolites, including 20S-PPD.

• Membrane and Water Treatment
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• 제6권3호
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• pp.237-249
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• 2015

In this study we investigate a laboratory scale vacuum membrane distillation system to produce pure water from model oil in water emulsion. Experimental determination of liquid entry pressure (LEP) of a commercial Durapore$^{TM}$ GVPH flat sheet membrane using model emulsions in various oil concentrations has been carried out. Two different methods of liquid entry pressure determination - a frequently used, so-called static and a novel dynamic method - have been investigated. In case of static method, LEP value was found to be 2.3 bar. No significant effect of oil content on LEP was detected up to 3200 ppm. In contrast, LEP values determined with dynamic method showed strong dependence on the oil concentration of the feed and decreased from 2.0 bar to a spontaneous wetting at 0.2 bar in the range 0-250 ppm, respectively. Vacuum membrane distillation tests were also performed. The separation performance is evaluated in terms of flux behavior, total organic carbon removal and droplet size distribution of the feed and final retentate. No significant effect of oil content on the flux was found ($5.05{\pm}0.31kgm^{-2}h^{-1}$) up to 250 ppm, where a spontaneous wetting occurred. High separation performance was achieved along with the increasing oil concentration between 93.4-97.0%.

• 한국지능시스템학회논문지
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• 제27권1호
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• pp.79-87
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• 2017

기상수치예보모델의 강수물리과정은 강수 발생과 연관된 입자의 낙하속도, 부착 및 자동전환, 입자크기분포 등의 과정을 다룬다. 하지만 수치예보모델의 미세물리과정과 모수에는 상당한 불확실성이 내포되어 있다. 수치예보모델의 불확실성을 줄이기 위하여 일반적으로 모수 추정을 사용한다. 이 연구에서는 모수 추정을 위한 최적화 알고리즘으로 마이크로 유전알고리즘과 하모니탐색 알고리즘을 사용하고 우리나라에서 발생한 강수사례에 대해 통합모델의 강수물리과정에서 사용하는 모수를 최적화하였다. 두 알고리즘의 서로 다른 특성으로 인해 최적화 과정 중의 차이가 보였다. 마이크로 유전알고리즘은 440회 수행 후 약 1.033의 적합도로 수렴하였고 하모니탐색 알고리즘은 60번 수행 후 약 1.031의 적합도로 수렴하였다. 이를 통해 하모니탐색 알고리즘이 마이크로 유전알고리즘보다 더 빨리 최적의 모수를 탐색하는 것을 알 수 있었다. 따라서 계산비용이 방대한 기상수치예보모델의 최적화 문제에서 빠른 시간 내에 최적의 모수를 탐색해야 한다면 하모니 탐색 알고리즘이 더 적합하다는 것을 확인하였다.

• 한국화재소방학회논문지
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• 제22권3호
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• pp.216-220
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• 2008

물분무소화설비는 화원의 냉각, 주변공기의 희석, 미세한 물방울의 증발에 의한 열차단과 같은 소화특성을 이용한다. 미분무수의 운동량은 입자가 큰 물방울에 비해 상대적으로 낮으며 화원의 침투능력도 효과적이지 못하다. 증발된 수증기에 의한 기여는 무게가 가볍고 밀도가 희박하여 화원에 일부분으로 제한되는 경향이 있다. 반면, 사이클식 미분무 패턴은 성층화된 분무 특성에 의해 공기 추출능력과 침투력이 개선될 것으로 예상된다. 이 논문에서는 유체공학적 화재모델인 FDS를 사용하여 교번식 미분무 패턴에 대해 분석된 소화능력을 다룬다. 저자는 이 분석이 미분무 노즐의 표본을 개발하기 위한 기본 개념을 제공할 수 있기를 기대한다.

• 대한수의학회지
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• 제38권4호
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• pp.882-897
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• 1998

This experiment was conducted to predict the effects of motional characteristics on the fertility of Korean native cattle(KNC) by using CASA technology and in vitro fertilization system. Twenty-six KNC frozen semen straws were obtained from Korean KNC improvement department, livestock improvement main division, national livestock cooperatives federation in Korea. Specimens were allowed to thaw at $37^{\circ}C$ for 30 sec in water bath. Semen analysis was performed on semen image analysis system(SIAS, Medical supply, Korea) adjusted to the gate settings and used the semen droplet ($5{\mu}l$) placed on Makler counting chamber(Sefi medical instrument, Israel) prewarmed at $37^{\circ}C$. The same person used the same micropipette to fill the Makler counting chamber. A total of 150 or more of sperms were analysed in each specimen by a single trained person by scanning at least 5 to 10 fields. The oocytes collection, in vitro maturation, IVF, in vitro culture and determination of the cleavage rate were performed by the technique, as described by Hwang et al (1997). Statistical analysis was done by linear regression with use of the Sigma plot program on a IBM personal computer. The cleavage rate in vitro fertilized oocyte was significantly correlated(P<0.05) with MOT, VCL, VSL, VAP, ALH, BCF and MAD, but not CON, LIN, STR, WOB, DNM, DNC and HYP in regressional analysis. The results show that some kinematic characteristics of frozen-thawed semen by CASA can be predict the fertility in in vitro model system.

• 대한화장품학회지
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• 제38권3호
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• pp.201-207
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• 2012

Oil-in-ethanol (O/E) 마이크로에멀젼을 물에 희석하여 얻은 O/W 나노에멀젼의 성질에 대하여 다른 희석 과정의 영향을 연구하였다. 물/에탄올/비이온성계면활성제/실리콘 오일 계를 모델 계로 선택하였다. 희석과정은 물(또는 마이크로에멀젼)을 마이크로에멀젼(또는 물)에 단계별로 첨가하는 방법으로 구성되었다. O/E 마이크로에멀젼을 물에 첨가하여 혼합하면 30 nm 정도의 입경을 가진 나노에멀젼을 얻을 수 있었다. 반면에 물을 O/E 마이크로에멀젼에 첨가하면 400 nm의 입경을 가진 에멀젼을 얻을 수 있다. 희석 방법이 얻어지는 에멀젼의 성질에 중요한 역할을 하였다. 시간에 따른 나노에멀젼의 입자 변화는 관찰되지 않았으나 입자가 큰 에멀젼은 시간에 따라 입경이 증가하였으며 불안정화 기작은 오스트왈드 라이퍼닝으로 추정되었다.

• Molecules and Cells
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• 제43권11호
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• pp.964-973
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• 2020

Recent studies have highlighted that early enhancement of the glycolytic pathway is a mode of maintaining the proinflammatory status of immune cells. Thiamine, a wellknown co-activator of pyruvate dehydrogenase complex, a gatekeeping enzyme, shifts energy utilization of glucose from glycolysis to oxidative phosphorylation. Thus, we hypothesized that thiamine may modulate inflammation by alleviating metabolic shifts during immune cell activation. First, using allithiamine, which showed the most potent anti-inflammatory capacity among thiamine derivatives, we confirmed the inhibitory effects of allithiamine on the lipopolysaccharide (LPS)-induced pro-inflammatory cytokine production and maturation process in dendritic cells. We applied the LPS-induced sepsis model to examine whether allithiamine has a protective role in hyper-inflammatory status. We observed that allithiamine attenuated tissue damage and organ dysfunction during endotoxemia, even when the treatment was given after the early cytokine release. We assessed the changes in glucose metabolites during LPS-induced dendritic cell activation and found that allithiamine significantly inhibited glucose-driven citrate accumulation. We then examined the clinical implication of regulating metabolites during sepsis by performing a tail bleeding assay upon allithiamine treatment, which expands its capacity to hamper the coagulation process. Finally, we confirmed that the role of allithiamine in metabolic regulation is critical in exerting anti-inflammatory action by demonstrating its inhibitory effect upon mitochondrial citrate transporter activity. In conclusion, thiamine could be used as an alternative approach for controlling the immune response in patients with sepsis.

• 한국대기환경학회:학술대회논문집
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• 한국대기환경학회 2006년도 춘계학술대회 논문집
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• pp.55-56
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• 2006

This thesis describes the use of measured aerosol size distributions and size-resolved hygroscopic growth to examine the physical and chemical properties of several particle classes. The primary objective of this work was to investigate the optical and cloud forming properties of a range of ambient aerosol types measured in a number of different locations. The tool used for most of these analyses is a differential mobility analyzer / tandem differential mobility analyzer (DMA / TDMA) system developed in our research group. To collect the data described in two of the chapters of this thesis, an aircraft-based version of the DMA / TDMA was deployed to Japan and California. The data described in two other chapters were conveniently collected during a period when the aerosol of interest came to us. The unique aspect of this analysis is the use of these data to isolate the size distributions of distinct aerosol types in order to quantify their optical and cloud forming properties. I used collected data during the Asian Aerosol Characterization Experiment (ACE-Asia) to examine the composition and homogeneity of a complex aerosol generated in the deserts and urban regions of China and other Asian countries. An aircraft-based tandem differential mobility analyzer was used for the first time during this campaign to examine the size-resolved hygroscopic properties of particles having diameters between 40 and 586 nm. Asian Dust Above Monterey (ADAM-2003) study was designed both to evaluate the degree to which models can predict the long-range transport of Asian dust, and to examine the physical and optical properties of that aged dust upon reaching the California coast. Aerosol size distributions and hygroscopic growth are measured in College Station, TX to investigate the cloud nucleating and optical properties of a biomass burning aerosol generated from fires on the Yucatan Peninsula. Measured aerosol size distributions and size-resolved hygroscopicity and volatility were used to infer critical supersaturation distributions of the distinct particle types that were observed during this period. The predicted CCN concentrations were used in a cloud model to determine the impact of the different aerosol types on the expected cloud droplet concentration. RH-dependent aerosol extinction coefficients are calculated at a wavelength of 550 nm.

• 한국항공우주학회지
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• 제30권8호
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• pp.21-29
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• 2002

본 연구는 다공벽과 공동을 사용한 피동제어법을 천음속 습공기 유동에서 발생하는 충격파와 경계층 간섭에 적응하였다. 지배방정식은 액적성장 방정식과 완전히 결합된 2차원, 비정상, 압축성 Navier-Stokes 방정식이며, 3차 오더 MUSCL 타입의 TVD 기법을 사용하였다. 또 난류모델로는 Baldwin-Lomax 모델을 적용하였다. 본 연구에서 적용한 제어법의 유용성을 조사하기 위해 유동의 전압손실과 충격파 변위의 시간의존성 거동을 해석하였다. 수치계산 결과로부터 본 연구의 피동제어기법을 통해 천음속 습공기 유동에서 발생하는 충격파/경계층 간섭으로 인한 전압손실이 상당히 감소하였고, 익에서 충격파 운동을 억제하는 것으로 나타났다. 또 다공영역의 위치가 본 연구의 제어법의 효과에 상당한 영향을 준다는 것이 발견하였다.

• 한국안전학회지
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• 제35권4호
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• pp.84-91
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• 2020

The Korean nuclear industry developed the SPACE (Safety and Performance Analysis Code for nuclear power plants) code and this code adpots two-phase flows, two-fluid, three-field models which are comprised of gas, continuous liquid and droplet fields and has a capability to simulate three-dimensional model. According to the revised law by the Nuclear Safety and Security Commission (NSSC) in Korea, the multiple failure accidents that must be considered for accident management plan of nuclear power plant was determined based on the lessons learned from the Fukushima accident. Generally, to improve the reliability of the calculation results of a safety analysis code, verification work for separate and integral effect experiments is required. In this reason, the goal of this work is to verify calculation capability of SPACE code for multiple failure accident. For this purpose, it was selected the experiment which was conducted to simulate a Multiple Steam Generator Tube Rupture(MSGTR) accident with Passive Auxiliary Feedwater System(PAFS) operation by Korea Atomic Energy Research Institute (KAERI) and focused that the comparison between the experiment results and code calculation results to verify the performance of the SPACE code. The MSGR accident has a unique feature of the penetration of the barrier between the Reactor Coolant System (RCS) and the secondary system resulting from multiple failure of steam generator U-tubes. The PAFS is one of the advanced safety features with passive cooling system to replace a conventional active auxiliary feedwater system. This system is passively capable of condensing steam generated in steam generator and feeding the condensed water to the steam generator by gravity. As the results of overall system transient response using SPACE code showed similar trends with the experimental results such as the system pressure, mass flow rate, and collapsed water level in component. In conclusion, it could be concluded that the SPACE code has sufficient capability to simulate a MSGTR accident.