• Applied Biological Chemistry
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• 제34권4호
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• pp.312-317
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• 1991

선복화(旋覆花)의 휘발성(揮發性) 성분(成分)을 동정(同定)하기 위하여 국내산(國內産) 및 중국산(中國産)을 시료(試料)로 사용(使用)하였다. 휘발성(揮發性) 성분(成分) 포집(捕集)은 SDE 방법(方法)을 사용(使用)하여 건조(乾燥)된 시료(試料) 100g중 국내산(國內産) 155.3 mg 중국산(中國産) 101.6 mg을 얻었다. 포집(捕集)된 휘발성(揮發性) 성분(成分)을 GC 및 GC/MS를 이용(利用)하여 hydrocarbon 류(類) 10, aldehyde 류(類) 7, alcohol 류(類) 18, ketone 류(類) 3, ester 류(類) 5, acid 류(類) 13 및 miscellaneous compounds 6 성분(成分)으로 모두 62 성분(成分)을 동정(同定)하였다. 또한, 이들 성분(成分)을 좀 더 세밀(細密)히 검토(檢討)코자 silica gel column chromatography에 의해 용출용매(溶出溶媒)의 극성(極性)을 변화(變化)시킴으로 성분조성(成分組成)을 세분(細分)하였다. 그 결과(結果) 국내산(國內産) 선복화(旋覆花)의 휘발성(揮發成) 성분중(成分中) 탄화수소(炭化水素) 화합물(化合物) 분획(分劃)이 16.85% 함산소화합물(含酸素化合物) 분획(分劃)이 83.15%이며, 중국산(中國産) 선복화(旋覆花)는 탄화수소(炭化水素) 화합물(化合物) 분획(分劃)이 23.46% 함산소화합물(含酸素化合物) 분획(分劃)이 76.53%를 함유(含有)하고 있다.

• 한국약용작물학회지
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• 제26권1호
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• pp.19-25
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• 2018

Background: Inula japonica Thunb. is a plant belonging to the family compositae. Inulae flos (flower of I. britannica var. chinensis Regal.) is the dried flower of I. japonica Thunb. and contains various flavonoids (patulitrin, nepitrin and kaempferol), which have been utilized in traditional oriental medicine to treat nausea, phlegm, and coughs. However, ethanol extract of I. britannica (IJE) has not been previously studied for its use in cancer treatment, and its effects on oxidative stress, or inflammation. Thus, the present study investigated the anti-oxidant, anti-inflammatory, and anti-colorectal cancer effects of IJE using RAW264.7 and HCT-116 cells, which are human colorectal cancer cell line. Methods and Results: IJE contained flavonoids ($80.95{\pm}5.3mg/g$) and polyphenols ($310.53{\pm}10.6mg/g$). Moreover, it reduced lipopolysaccharide (LPS)-induced nitric oxide (NO) production and $H_2O_2$-induced oxidative stress by decreasing reactive oxygen species (ROS) levels. Additionally, the $500{\mu}g/m{\ell}$ IJE treatment increased caspase-3 activity and apoptotic cell death in HCT-116 cells. Conclusions: These results demonstrate that the anti-cancer effect of IJE against human colorectal cancer cells involves caspase-3 activation and apoptotic cell death. IJE also inhibited LPS-induced NO production, and $H_2O_2$-induced oxidative stress in RAW264.7 cells. However, further studies are required to explore how IJE treatment regulates signal transduction in NO and ROS production.