• Journal of the Korean Chemical Society
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• v.55 no.3
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• pp.436-443
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• 2011

The solid complexes of La(III), Ce(III), Pr(III), Nd(III), Sm(III) and Gd(III) with 4-hydroxy-3-(1-{2-(2,4-dihydroxy-benzylidene)-amino phenylimino}-ethyl)-6-methyl-pyran-2-one ($H_2$L) derived from o-phenylenediamine, 3-acetyl-6-methyl-(2H)pyran,2,4(3H)-dione (dehydroacetic acid or DHA) and 2, 4-dihydroxy benzaldehyde have been synthesized and characterized by elemental analysis, conductometry, magnetic susceptibility, UV-visible, FT-IR, $^1H$-NMR, X-ray diffraction, thermal analysis study, and screened for antimicrobial activity. The FT-IR spectral data suggest that the ligand behaves as a dibasic tetradentate ligand with ONNO donor atoms sequence towards central metal ion. From the microanalytical data, the stoichiometry of the complexes has been found to be 1:1 (metal: ligand). The physico-chemical data suggests distorted octahedral geometry for La(III), Ce(III), Pr(III), Nd(III), Sm(III) and Gd(III) complexes. The X-ray diffraction data suggests monoclinic crystal system for La(III) and Ce(III) and orthorombic crystal system for Pr(III) and Nd(III) complexes. Thermal behavior (TG/DTA) of the complexes was studied and kinetic parameters were determined by Horowitz-Metzger and Coats-Redfern methods. The ligand and its metal complexes were screened for antibacterial activity against Staphylococcus aureus, Escherichia coli, Bacillus Sp. and fungicidal activity against Aspergillus Niger, Trichoderma and Fusarium oxysporum.

• Bulletin of the Korean Space Science Society
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• 2009.10a
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• pp.35.1-35.1
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• 2009

We have established a database of galaxy spectral line strengths for the SDSS database using an improved line measuring method. Our work includes the entire SDSS DR7 galaxies within redshift of 0.2. The absorption line strengths measured by the SDSS pipeline are seriously contaminated by emission filling. Our code, GANDALF (gas and absorption line fitting code) performs more accurate measurements by effectively separating emission lines from absorption lines. A significant improvement has also been made on the velocity dispersion measurement, more notably in late-type galaxies. We have also identified a number of broad line region galaxies which were misclassified as normal galaxies by the SDSS pipeline. We developed an effective method measuring their line strengths. The database will be provided with new parameters that are indicative of the line strength measurement quality. In addition, we made galaxy templates for the Hubble sequence. The database will be useful for many fields of galaxy studies including star formation and AGN activities.

• Development and Reproduction
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• v.18 no.4
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• pp.275-286
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• 2014

To successful molecular breeding, identification and functional characterization of breeding related genes and development of molecular breeding techniques using DNA markers are essential. Although the development of a useful marker is difficult in the aspect of time, cost and effort, many markers are being developed to be used in molecular breeding and developed markers have been used in many fields. Single nucleotide polymorphisms (SNPs) markers were widely used for genomic research and breeding, but has hardly been validated for screening functional genes in olive flounder. We identified single nucleotide polymorphisms (SNPs) from expressed sequence tag (EST) database in olive flounder; out of a total 4,327 ESTs, 693 contigs and 514 SNPs were detected in total EST, and these substitutions include 297 transitions and 217 transversions. As a result, 144 SNP markers were developed on the basis of 514 SNP to selection of useful gene region, and then applied to each of eight wild and culture olive flounder (total 16 samples). In our experimental result, only 32 markers had detected polymorphism in sample, also identified 21 transitions and 11 transversions, whereas indel was not detected in polymorphic SNPs. Heterozygosity of wild and cultured olive flounder using the 32 SNP markers is 0.34 and 0.29, respectively. In conclusion, we identified SNP and polymorphism in olive flounder using newly designed marker, it supports that developed markers are suitable for SNP detection and diversity analysis in olive flounder. The outcome of this study can be basic data for researches for immunity gene and characteristic with SNP.

• Childhood Kidney Diseases
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• v.4 no.1
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• pp.33-39
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• 2000

Purpose: Our study was designed to investigate the association of MHC Class II (DR, DQ) allele with IgA nephropathy and its significance as a prognostic factor for progression to ESRD Material and Methods: 69 children with IgA nephropathy with normal renal function(serum creatinine $\leq$ 1.5mg/dL) was classified as group A and 70 patients who received renal transplantation due to IgA nephropathy were selected as group B. The HLA-DQB1 and HLA-DRB1 alleles were studied by polymerase chain reaction using sequence specific primers. We have compared the difference in alleles between these two groups and with normal control and also examined any possible effect of the MHC class II genes on the histopathological severity and prognosis of IgAN. Results: Mean age was $8.8{\pm}2.9$ years in group A and $35.0{\pm}15.5$ years in group B. Male to female ratio was 2.8:1 in group A and 2.5:1 in group B. There was a significantly higher frequency of HLA-$DQB1^*03\;and\;DQB1^*05$ in Group B. The frequency of HLA-$DQB1^*0302\;and\;^*05031$ allele had increasing tendency in Group B(P<0.05). HLA-$DRB1^*03\;and\;^*05$ were more common in Group B(P<0.05). HLA-$DRB1^*04$ allele was the most common DR alleles in both group, but there was no statistical significance. There were no significant correlation with MHC class 13 genes on the hjstopathological severity in Group A. Conclusion: In conclusion, $HLA-DQB1^*0302\;and\;HLA-DQB1^*05031 $ allele seemed to be more common in transplanted patients compared to group with normal renal function suggesting that this allele is associated with poor prognosis in IgAN. However larger studies and follow up are required to confirm this due to uncharacterized heterogeneity in etiopathogenesis of IgA nephropathy and possibly one or more than one gene may exert influence in determining susceptibility to the diseases.

• Journal of Microbiology and Biotechnology
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• v.20 no.6
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• pp.995-1000
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• 2010

A novel deoC gene was identified from Paenibacillus sp. EA001 isolated from soil. The gene had an open reading frame (ORF) of 663 base pairs encoding a protein of 220 amino acids with a molecular mass of 24.5 kDa. The amino acid sequence was 79% identical to that of deoxyribose 5-phosphate aldolase (DERA) from Geobacillus sp. Y412MC10. The deoC gene encoding DERA was cloned into an expression vector and the protein was expressed in Escherichia coli. The recombinant DERA was purified using Ni-NTA affinity chromatography and then characterized. The optimum temperature and pH of the enzyme were $50^{\circ}C$ and 6.0, respectively. The specific activity for the substrate deoxyribose 5-phosphate (DR5P) was $62\;{\mu}mol/min/mg$. The $K_m$ value for DR5P was determined to be 145 mM with the $k_{cat}$ value of $3.2{\times}10^2/s$ from Lineweaver-Burk plots. The EA001 DERA showed stability toward a high concentration of acetaldehyde (100 mM).

• Journal of Korean Neurosurgical Society
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• v.46 no.6
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• pp.558-563
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• 2009

Objective : Moyamoya disease (MMD) is an uncommon cerebrovascular disorder, characterized by progressive occlusion at the terminal portion of the internal carotid artery. Incidence of the disease is high in East Asia and familial MMD accounts for about 15% of the disease. Although the pathogenesis is unknown, association of HLA class I or II alleles with MMD has been reported with conflicting results. We investigated whether there is a difference in HLA class II association between familial and non-familial forms of the disease. Methods : A total of 70 Korean children with MMD, including 16 familial cases (10 probands), and 207 healthy controls were studied. Among familial cases, only 10 probands were used for the HLA frequency analysis. High resolution HLA-DRB1 and DQB1 genotyping was performed using polymerase chain reaction (PCR)-sequence specific oligonucleotide hybridization and PCR-single strand conformation polymorphism methods. Results : The phenotype frequencies of HLA-DRB1*1302 (70.0%) and DQB1*0609 (40.0%) were significantly increased in familial MMD compared to both controls [vs. 15.5%, corrected p ($p_c$) = 0.008, odds ratio (OR) = 12.76; vs. 4.3%, $p_c\;=\;0.02$, OR = 14.67] and non-familial MMD patients (vs. 14.8%, $p_c\;=\;0.02$, OR = 13.42; vs. 1.9%, $p_c\;=\;0.02$, OR = 35.33). The frequencies of DRB1 and DQB1 alleles in non-familial MMD patients were not significantly different from those in controls. Conclusion : Our findings suggest that the genetic polymorphism of HLA class II genes or other closely linked disease relevant gene(s) could be a genetic predisposing factor for familial MMD.

• KSBB Journal
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• v.19 no.6 s.89
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• pp.462-466
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• 2004

Mycothiol (MSH), a low molecular antioxidant thiol compound, was purified and analyzed from Streptomyns coelicolor A[3]2 by the monobromobimane fluorescence detection method modified by this lab. Through HPLC chromatpgram, MSH fraction was obtained following the elution time of standard MSH (donated by Dr. Robert C. Fahey). That MSH showed the highest concentration among the thiol compounds contained in the cell indicated that MSH was the key thiol compound having antioxidant activity. To understand the role of gene of inositol monophosphatase (I-1-Pase) involved in the MSH biosynthesis, it was isolated from S. coelicolor A(3)2 and cloned and overexpressed in the Escherichia coli. The expressed I-1-Pase was purified through Ni-NTA column. The soluble protein consisted of 281 amino acids, and the molecular weight was 32 kDa. I-1-Pase of S. coelicolor A(3)2 had the sequence homology with those of human and E. coli by 24 and $25\%$, respectively, and had two conserved domains (mofif A and motif B) which were typical of I-1-Pase.

• The Bulletin of The Korean Astronomical Society
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• v.37 no.1
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• pp.48.1-48.1
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• 2012

We present the ultraviolet (UV) color-color relation of early-type galaxies (ETGs) in the nearby universe (0.05 < z < 0.12) to investigate the properties of hot stellar populations responsible for the UV excess (UVX). The initial sample of ETGs is selected by the spectroscopic redshift and the morphology parameter from the SDSS DR 7, and then cross-matched with the GALEX far-UV (FUV) and near-UV (NUV) GR6 data. The cross-matched ETG sample is further classified by their emission line characteristics in the optical spectra into quiescent, star-forming, and active galactic nucleus categories. Contaminations from early-type spiral galaxies, mergers, and morphologically disturbed galaxies are removed by visual inspection. By drawing the FUV-NUV (as a measure of UV spectral shape) versus FUV-r (as a measure of UVX strength) diagram for the final sample of -3700 quiescent ETGs, we find that the "old and dead" ETGs consist of a well-defined sequence in UV colors, the "UV red sequence," so that the stronger UVX galaxies should have a harder UV spectral shape systematically. However, the observed UV spectral slope is too steep to be reproduced by the canonical stellar population models in which the UV flux is mainly controlled by age or metallicity parameters. Moreover, 2 mag of color spreads both in FUV-NUV and FUV-r appear to be ubiquitous among any subsets in distance or luminosity. This implies that the UVX in ETGs could be driven by yet another parameter which might be even more influential than age or metallicity.

• The Transactions of the Korean Institute of Power Electronics
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• v.4 no.5
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• pp.423-432
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• 1999

This paper proposes a 12­pulse diode rectifier system with low kVA components suitable for powering switch mode power supplies or ac/dc converter applications. The proposed 12-pulse system employs a polyphase transformer, a zero sequence blocking transformer (ZSBT) in the dc link, and an interphase transformer. Results produce near equal leakage inductance in series with each diode rectifier bridge ensuring equal current sharing and performance improvements, The utility input currents and the voltage across the ZSBT are analyzed the kVA rating of each component in the proposed system is computed. The 5th , 7th , 17th and 19th harmonics are eliminated in the input line currents resulting in clean input power. The dc link voltage magnitude generated by the proposed rectifier system is nearly identical to a conventional to a conventional 6-pulse system. The proposed system is suitable to retrofit applications as well as in new PWM drive systems. Simulation and experimental results from a 208V , 10kVA system are shown.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.40.1-40.1
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• 2014

We have classified barred galaxies for 418 RC3 sample galaxies within z < 0.01 from SDSS DR7 using the visual inspection, ellipse fitting method and Fourier analysis. We found the bar fraction to be ~60%, 43% and 70% for each method and that the ellipse fitting method tends to miss the bar when a large bulge hides the transition from bar to disk in early spirals. We also confirmed that the Fourier analysis cannot distinguish between a bar and spiral arm structure. These systematic difficulties may have produced the long-time controversy about bar fraction dependence on Hubble sequence, mass and color. We designed a new method to fine bars by analyzing the ratio map of bar strength in polar coordinates, which yields the bar fraction of ~27% and ~32% for SAB and SB, respectively. The consistency with visual inspection reaches around 70%, and roughly 90% of visual strong bar are classified as SAB and SB in our classification. Although our method also has a weakness that a large bulge lowers the value of bar strength, the missing bar fraction in early spirals is reduced to the level of ~1/4 compared to the ellipse fitting method. Our method can make up for the demerits of the previous automatic classifications and provide a quantitative bar classification that agrees with visual classification.