• 한국항공우주학회지
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• 제48권11호
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• pp.889-902
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• 2020

본 연구에서는 직접 연결식 초음속 연소기에서 설계점을 만족하는 초음속 유동의 형성유무 및 안정화 소요시간을 확인하기 위한 수치해석이 수행되었다. 이를 위하여 연소식 공기 가열기 하류의 고압 유동이 초음속 연소기로 전파되며 초음속 유동장을 형성해가는 과정을 살펴보았다. 압력 및 온도 분석을 통해, 초음속 유동장이 설계점인 마하수 2.0, 1,000 K을 만족하며, 최소 4.0 ms의 안정화 시간이 필요함을 확인하였다. 따라서 초음속 연소시험에서 연료분사 이전에 유동 안정화에 필요한 시간을 고려해야 함을 알 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제4권6호
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• pp.487-496
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• 2000

Insulin stimulates glucose transport in muscle and fat cells by promoting the translocation of glucose transporter (GLUT4) to the cell surface. Phosphatidylinositide 3-kinase (PI3-kinase) has been implicated in this process. However, the involvement of protein kinase B (PKB)/Akt and $PKC-{\zeta}$, those are known as the downstream target of PI3-kinase in regulation of GLUT4 translocation, is not known yet. An interesting possibility is that these protein kinases phosphorylate GLUT4 directly in this process. In the present study, $PKB-{\alpha}$ and $PKC-{\zeta}$ were added exogenously to GLUT4-containing vesicles purified from low density microsome (LDM) of the rat adipocytes by immunoadsorption and immunoprecipitation for direct phosphorylation of GLUT4. Interestingly GLUT4 was phosphorylated by $PKC-{\zeta}$ and its phosphorylation was increased in insulin stimulated state but GLUT4 was not phosphorylated by $PKB-{\alpha}.$ However, the GST-fusion proteins, GLUT4 C-terminal cytoplasmic domain (GLUT4C) and the entire major GLUT4 cytoplasmic domain corresponding to N-terminus, central loop and C-terminus in tandem (GLUT4NLC) were phosphorylated by both $PKB-{\alpha}$ and $PKC-{\zeta}.$ The immunoblots of $PKC-{\zeta}$ and $PKB-{\alpha}$ antibodies with GLUT4-containing vesicles preparation showed that $PKC-{\zeta}$ was co-localized with the vesicles but not $PKB-{\alpha}.$ From the above results, it is clear that $PKC-{\zeta}$ interacts with GLUT4-containing vesicles and it phosphorylates GLUT4 protein directly but $PKB-{\alpha}$ does not interact with GLUT4, suggesting that insulin-elicited signals that pass through PI3-kinase subsequently diverge into two independent pathways, an Akt pathway and a $PKC-{\zeta}$ pathway, and that later pathway contributes, at least in part, insulin stimulation of GLUT4 translocation in adipocytes via a direct GLUT4 phosphorylation.

• Molecules and Cells
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• 제39권10호
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• pp.768-775
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• 2016

The Arabidopsis female gametophyte contains seven cells with eight haploid nuclei buried within layers of sporophytic tissue. Following double fertilization, the egg and central cells of the gametophyte develop into the embryo and endosperm of the seed, respectively. The epigenetic status of the central cell has long presented an enigma due both to its inaccessibility, and the fascinating epigenome of the endosperm, thought to have been inherited from the central cell following activity of the DEMETER demethylase enzyme, prior to fertilization. Here, we present for the first time, a method to isolate pure populations of Arabidopsis central cell nuclei. Utilizing a protocol designed to isolate leaf mesophyll protoplasts, we systematically optimized each step in order to efficiently separate central cells from the female gametophyte. We use initial manual pistil dissection followed by the derivation of central cell protoplasts, during which process the central cell emerges from the micropylar pole of the embryo sac. Then, we use a modified version of the Isolation of Nuclei TAgged in specific Cell Types (INTACT) protocol to purify central cell nuclei, resulting in a purity of 75-90% and a yield sufficient to undertake downstream molecular analyses. We find that the process is highly dependent on the health of the original plant tissue used, and the efficiency of protoplasting solution infiltration into the gametophyte. By isolating pure central cell populations, we have enabled elucidation of the physiology of this rare cell type, which in the future will provide novel insights into Arabidopsis reproduction.

• Corrosion Science and Technology
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• 제7권6호
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• pp.350-361
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• 2008

Canada's oil sands contain one of the largest reserves of oil in the world. According to recent estimates, there are nearly 180 billion barrels of oil in the Canadian oil sands trapped in a complex mixture of sand, water and clay. More than 40 companies have been currently operating or developing oil sands facilities since the first production in 1967. The process of oil sands upgrading is similar with down stream refinery, but the corrosion environment in upgrading refinery is often more severe than in the refinery because of high chlorides, mineral contents, carbonic acid, heavy viscosity and fouling, higher naphthenic acid [$NA-R(CH_{2})nCOOH$], and greater sulfur contents. Naphthenic acid corrosion (NAC) which is one of the most critical corrosion issues in up & downstream refinery plants was observed for the first time in 1920's in refinery distillation processes of Rumania, Azerbaizan (Baku), Venezuela, and California. As a first API report, the 11th annual meeting stated sources and mechanism of NAC in early 1930's. API has been developing the risk base standards, such as API RP580, 571, and Publication 581 which are based on the worst NAC damage in the world since 2000. Nevertheless not only the NAC phenomena and control in Canadian sands oil process are not much widely known but also there are still no engineering guidances for the Canadian sands oil in API standards. This paper will give NAC phenomina and materials selection guidance against NA environment in Canadian oil sands upgrading processes.

• Nuclear Engineering and Technology
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• 제45권2호
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• pp.257-264
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• 2013

In Canada Deuterium Uranium (CANDU)-type nuclear power plants, the reactor is composed of 380 fuel channels and refueling is performed on one or two channels per day. At the time of refueling, the fluid force of the cooling water inside the channel is exploited. New fuel added upstream of the fuel channel is moved downstream by the fluid force of the cooling water, and the used fuel is pushed out. Through this process, refueling is completed. Among the 380 fuel channels, outer rows 1 and 2 (called the FARE channel) make the process of using only the internal fluid force impossible because of the low flow rate of the channel cooling water. Therefore, a Flow Assist Ram Extension (FARE) tool, a refueling aid, is used to refuel these channels in order to compensate for the insufficient fluid force. The FARE tool causes flow resistance, thus allowing the fuel to be moved down with the flow of cooling water. Although the existing FARE tool can perform refueling in Korean plants, the coolant flow rate is reduced to below 80% of the normal flow for some time during refueling. A Flow rate below 80% of the normal flow cause low flow rate alarm signal in the plant operation. A flow rate below 80% of the normal flow may cause difficulties in the plant operation because of the increase in the coolant temperature of the channel. A new and improved FARE tool is needed to address the limitations of the existing FARE tool. In this study, we identified the cause of the low flow phenomena of the existing FARE tool. A new and improved FARE tool has been designed and manufactured. The improved FARE tool has been tested many times using laboratory test apparatus and was redesigned until satisfactory results were obtained. In order to confirm the performance of the improved FARE tool in a real plant, the final design FARE tool was tested at Wolsong Nuclear Power Plant Unit 2. The test was carried out successfully and the low flow rate alarm signal was eliminated during refueling. Several additional improved FARE tools have been manufactured. These improved FARE tools are currently being used for Korean CANDU plant refueling.

• Molecules and Cells
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• 제39권4호
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• pp.345-351
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• 2016

Wound healing is a complex physiological process necessitating the coordinated action of various cell types, signals and microRNAs (miRNAs). However, little is known regarding the role of miRNAs in mediating this process. In the present study, we show that let-7c miRNA is decreased in heat-denatured fibroblasts and that inhibiting let-7c expression leads to the increased proliferation and migration of dermal fibroblasts, whereas the overexpression of let-7c exerts an opposite effect. Further investigation has identified heat shock protein 70 as a direct target of let-7c and has demonstrated that the expression of HSP70 in fibroblasts is negatively correlated with let-7c levels. Moreover, down-regulation of let-7c expression is accompanied by up-regulation of Bcl-2 expression and down-regulation of Bax expression, both of which are the downstream genes of HSP70. Notably, the knockdown of HSP70 by HSP70 siRNA apparently abrogates the stimulatory effect of let-7c inhibitor on heat-denatured fibroblasts proliferation and migration. Overall, we have identified let-7c as a key regulator that inhibits fibroblasts proliferation and migration during wound healing.

• Molecules and Cells
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• 제41권12호
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• pp.1072-1080
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• 2018

A plant-specific B3 domain and AP2 domain-containing transcription factor, RAV1 acts as a negative regulator of growth in many plant species and its transcription was down-regulated by BR and ABA. In this study, we found that RAV1-overexpressing transgenic plants showed abnormally developed ovules, resulting in reduced seed size, weight, and number in a silique. Interestingly, the endogenous expression of RAV1 fluctuated during seed development; it remained low during the early stage of seed development and sharply increased in the seed maturation stage. In plants, seed development is a complex process that requires coordinated growth of the embryo, endosperm, and maternal integuments. Among many genes that are associated with endosperm proliferation and embryo development, three genes consisting of SHB1, MINI3, and IKU2 form a small unit positively regulating this process, and their expression was regulated by BR and ABA. Using the floral stage-specific RNAs, we found that the expression of MINI3 and IKU2, the two downstream genes of the SHB1-MINI3-IKU2 cascade in the seed development pathway, were particularly reduced in the RAV1-overexpressing transgenic plants. We further determined that RAV1 directly binds to the promoter of MINI3 and IKU2, resulting in their repression. Direct treatment with brassinolide (BL) improved seed development of RAV1-overexpressing plants, but treatment with ABA severely worsened it. Overall, these results suggest that RAV1 is an additional negative player in the early stages of seed development, during which ABA and BR signaling are coordinated.

• The Korean Journal of Physiology and Pharmacology
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• 제23권5호
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• pp.357-366
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• 2019

$G{\alpha}_q$-coupled receptor stimulation was implied in the activation process of transient receptor potential canonical (TRPC)1/4 and TRPC1/5 heterotetrameric channels. The inactivation occurs due to phosphatidylinositol 4,5-biphosphate ($PI(4,5)P_2$) depletion. When $PI(4,5)P_2$ depletion was induced by muscarinic stimulation or inositol polyphosphate 5-phosphatase (Inp54p), however, the inactivation by muscarinic stimulation was greater compared to that by Inp54p. The aim of this study was to investigate the complete inactivation mechanism of the heteromeric channels upon $G{\alpha}_q$-phospholipase $C{\beta}$ ($G{\alpha}_q-PLC{\beta}$) activation. We evaluated the activity of heteromeric channels with electrophysiological recording in HEK293 cells expressing TRPC channels. TRPC1/4 and TRPC1/5 heteromers undergo further inhibition in $PLC{\beta}$ activation and calcium/protein kinase C (PKC) signaling. Nevertheless, the key factors differ. For TRPC1/4, the inactivation process was facilitated by $Ca^{2+}$ release from the endoplasmic reticulum, and for TRPC1/5, activation of PKC was concerned mostly. We conclude that the subsequent increase in cytoplasmic $Ca^{2+}$ due to $Ca^{2+}$ release from the endoplasmic reticulum and activation of PKC resulted in a second phase of channel inhibition following $PI(4,5)P_2$ depletion.

• 멤브레인
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• 제32권5호
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• pp.348-356
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• 2022

바이오산업의 발전으로 의약품, 식품 등의 생산 과정의 분리/정제 공정에 사용되어 왔던 기존의 컬럼 크로마토그래피를 대체하여 더 높은 처리효율을 갖는 막 크로마토그래피가 부상하고 있다. 본 연구에서는 서로 다른 기공 크기의 두 가지 상용 셀룰로오스 아세테이트(Cellulose acetate, CA) 분리막을 탈아세틸화 과정을 통해, 리간드의 개질이 용이한 다공성 재생 셀룰로오스 지지체를(Regenerated cellulose, RC) 제조하였다. 음이온 교환능을 부여하고자 grafting을 수행하였으며, 구체적으로는 UV 중합법을 통해 4차 암모늄을 포함하는 음이온 교환 리간드(MAPTAC)를 부착하여 음이온 교환용 흡착막을 제조하였다. 단백질 흡착 용량은 정적 흡착 용량(Static binding capacity, SBC)시험을 통해 총 단백질 흡착 용량을 측정했고, 동적 흡착 용량(Dynamic binding capacity, DBC)을 측정하여 상용막과 비교 평가하였다. 성능 평가 결과 단백질 흡착량은 넓은 표면적에 의해 리간드 밀도가 높은, 기공 크기가 작은 순서로 높게 측정되었고, 상용 CA분리막을 탈아세틸화하고 리간드를 부착시킨 분리막(RC 0.8 + MAPTAC 43.69 mg/ml, RC 3.0 + MAPTAC 36.33 mg/ml)이 상용 막 크로마토그래피 제품(28.38 mg/ml) 대비 높은 흡착 용량을 보였다.

• 한국지구과학회지
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• 제34권6호
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• pp.492-506
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• 2013

최근 백두산의 재분화 문제가 하나의 중요한 지구과학적 문제로 부상하고 있다. 백두산과 같이 대규모 칼데라 호수를 갖고 있는 화산의 폭발은 단순히 화산분출뿐만 아니라 칼테라 호수에 저장된 물의 유출로 인해 큰 재해를 유발할 수 있어 연구가 필요하다. 대형 화산성 홍수는 모든 종류의 인공 구조물을 파괴할 수 있는 에너지를 가지고 있고, 유속이 100 km $hr^{-1}$에 달하며, 도달거리가 수 백 km까지 이르기 때문에 치명적인 피해를 가져올 수 있다. 연구의 최종목표는 백두산의 지질과 지반 조건에서 분화 시 예상되는 화산성 홍수의 피해를 예측하는 것이다. 하나의 사전 연구로서 백두산 화산이 분출되면서 천지에 저장된 물이 방류될 경우를 가정한 유량곡선 시나리오에 근거하여 수치해석을 실시하였다. 각각의 시나리오 별(림 붕괴, 단순융기, 림 붕괴와 융기의 조합, 강우형성 등)로 시간의 함수로서 유량변화를 이끌어내기 위해 선행 연구를 바탕으로 백두산 천지 유출 모형을 전개하였다. 천지에서 마그마 융기와 림 붕괴가 동시에 발생하면 최고 유량이 25,000 $m^3s^{-1}$에 이르러 백두산 천지의 화산홍수가 주변 지역에 심각한 자연재해를 가져올 수 있는 것으로 보인다. 이 연구에서는 저수지의 물이 방류되는 순간유량곡선에 치중하였으며 천지 유량 방출 후 하류 하천 하도 추적은 다루지 않았다.