• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.277.1-277.1
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• 2013

Molecular diagnostics consists of three processes, which are a sample pretreatment, a nucleic acid amplification, and an amplicon detection. Among three components, sample pretreatment is an important process in that it can increase the limit of detection by purifying nucleic acid in biological sample from contaminants that may interfere with the downstream genetic analysis such as nucleic acid amplification and detection. To achieve point-of-care virus detection system, the sample pretreatment process needs to be simple, rapid, and automatic. However, the commercial RNA extraction kits such as Rneasy (Qiagen) or MagnaPure (Roche) kit are highly labor-intensive and time-consuming due to numerous manual steps, and so it is not adequate for the on-site sample preparation. Herein, we have developed a rotary microfluidic system to extract and purify the RNA without necessity of external mechanical syringe pumps to allow flow control using microfluidic technology. We designed three reservoirs for sample, washing buffer, and elution buffer which were connected with different dimensional microfluidic channels. By controlling RPM, we could dispense a RNA sample solution, a washing buffer, and an elution buffer successively, so that the RNA was captured in the sol-gel solid phase, purified, and eluted in the downstream. Such a novel rotary sample preparation system eliminates some complicated hardwares and human intervention providing the opportunity to construct a fully integrated genetic analysis microsystem.

• International Journal of Naval Architecture and Ocean Engineering
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• 제13권1호
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• pp.236-245
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• 2021

In this study, direct numerical and large eddy simulations of transitional flows around studs were conducted to investigate the effectiveness of turbulence stimulators at very low speeds for the minimum propulsion power condition of four knots. For simplicity, the studs were assumed to be installed on a flat plate, while the wake was observed up to 0.23 m downstream behind the second stud. For applicability to a model ship, we also studied the flow characteristics behind the first and second studs installed on a curved plate, which was designed to describe the geometry of a bulbous bow. A laminar-to-turbulent transition was observed in the wake at Re_D ≥ 921 (U_∞≥0.290 m/s), and the wall shear stress at Re_D = 1162 (U_∞ = 0.366 m/s) in the second wake was similar to that of the fully developed turbulent boundary layer after a laminar-to-turbulent transition in the first wake. At Re_D = 581 (U_∞ = 0.183 m/s), no turbulence was stimulated in the wake behind the first and second studs on the flat plate, while a cluster of vortical structures was observed in the first wake over the curved plate. However, a cluster of vortical structures was revealed to be generated by the reattachment process of the separated shear layer, which was disturbed by the first stud rather than directly initiated by the first stud. It was quite different from a typical process of transition, which was observed at relatively high Re_D that the spanwise scope of the turbulent vortical structures expanded gradually as it went downstream.

• 한국환경과학회지
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• 제22권7호
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• pp.905-913
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• 2013

We compared the applicability and economical efficiency of peroxone process with those of ozone process in the existing water treatment plant on downstream of Nakdong River. After comparing the peroxone process for removing geosmin with the ozone process in lab scale test, peroxone process showed much higher removal efficiency than the ozone process at the same ozone dosage. Proper range of $H_2O_2/O_3$ ratio were 0.5~1.0 and the half-life of geosmin was about 5.5~6.8 min when the $H_2O_2/O_3$ ratio was set to 0.5 during 1~2 mg/L of ozone dosage. Peroxone process could reduce the ozone dosage about 50 to maximum 30% for the same geosmin removal efficiency compared to the ozone process in the pilot scale test. In case of 1,4-dioxane treatment, peroxone process could have 3~4 times higher efficiency than ozone process at the same ozone dosage. The results of estimating the economical efficiency of ozone and peroxone process for treating geosmin and 1,4-dioxane by using pilot scale test, in case of the removal target was set to 85% for these two materials, the cost of peroxane process could be reduced about 1.5 times compared to ozone process, and in the same production cost peroxone process could have 2~3 times higher removal efficiency than ozone process. The removal efficiency by peroxone process showed a large difference depending on the physicochemical characteristics of target materials and raw water, therefore detailed examination should be carried out before appling peroxone process.

• 한국수자원학회논문집
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• 제44권9호
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• pp.765-776
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• 2011

댐이 건설된 이후 댐직하류 하천구간은 하천구역내 사유지 경작과 하상주차장 등의 입지, 사주가 발달하여 하폭감소에 의한 문제가 발생하였다. 특히 하천정비의 미흡으로 홍수시 하천통수능력이 감소하여 유역전반에 영향을 미치고 있다. 댐 운영조건도 일부 제약을 받고 있는데 이의 해소를 통한 댐 기능의 회복과 운영의 효율성 제고가 하천의 치수 안정성 확보와 함께 개선대책이 필요하였다. 따라서 댐 운영관리개선과 연계된 댐직하류 하천정비사업이 시행되고 있는데 사업의 타당성, 경제적 편익, 비용배분 및 투자비 회수방안 등과 같은 문제점이 제기되었다. 아울러 댐사업자가 하천사업을 주관한 사례가 없어 이들 사업에 대한 경제성 평가의 객관적 해석과 편익에 대한 정량화 방법의 개발이 요구되었다. 본 연구는 댐직하류 하천정비사업의 사업주체(국가와 용수 및 발전사업자 등)간의 합리적인 건설비용 배분방법을 제시하고 그 적정성을 평가하고자 하였다. 평가결과, 시범 적용 3개 댐의 경제적 편익은 14,405.8백만원/년으로 추정되었고, 편익 중 사업시행자인 K-water의 재무적 가치는 40%, 정부의 공공성 편익은 60%로 분석되었다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권8호
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• pp.1095-1103
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• 2019

Objective: Among stress responses, the unfolded protein response (UPR) is a well-known mechanism related to endoplasmic reticulum (ER) stress. ER stress is induced by a variety of external and environmental factors such as starvation, ischemia, hypoxia, oxidative stress, and heat stress. Inositol requiring enzyme $1{\alpha}$ ($IRE1{\alpha}$)-X-box protein 1 (XBP1) is the most conserved pathway involved in the UPR and is the main component that mediates $IRE1{\alpha}$ signalling to downstream ER-associated degradation (ERAD)- or UPR-related genes. XBP1 is a transcription factor synthesised via a novel mechanism called 'frame switch splicing', and this process has not yet been studied in the horse XBP1 gene. Therefore, the aim of this study was to confirm the frame switch splicing of horse XBP1 and characterise its dynamics using Thoroughbred muscle cells exposed to heat stress. Methods: Primary horse muscle cells were used to investigate heat stress-induced frame switch splicing of horse XBP1. Frame switch splicing was confirmed by sequencing analysis. XBP1 amino acid sequences and promoter sequences of various species were aligned to confirm the sequence homology and to find conserved cis-acting elements, respectively. The expression of the potential XBP1 downstream genes were analysed by quantitative real-time polymerase chain reaction. Results: We confirmed that splicing of horse XBP1 mRNA was affected by the duration of thermal stress. Twenty-six nucleotides in the mRNA of XBP1 were deleted after heat stress. The protein sequence and the cis-regulatory elements on the promoter of horse XBP1 are highly conserved among the mammals. Induction of putative downstream genes of horse XBP1 was dependent on the duration of heat stress. We confirmed that both the mechanisms of XBP1 frame switch splicing and various binding elements found in downstream gene promoters are highly evolutionarily conserved. Conclusion: The frame switch splicing of horse XBP1 and its dynamics were highly conserved among species. These results facilitate studies of ER-stress in horse.

• 한국기계가공학회지
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• 제17권2호
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• pp.8-14
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• 2018

In the present work, a numerical study is carried out to observe the characteristics of the flow and particle behaviors in a supersonic double Venturi abrasive blasting nozzle. Schlieren flow visualization and Pitot pressure at the nozzle downstream are also carried out, and those measurement results are compared to the numerical ones for code validation. Open and closed secondary holes on the double Venturi nozzle surface are tested for various nozzle pressures, and the results are compared with the ones observed for other similar supersonic Laval nozzles.

• Journal of Microbiology and Biotechnology
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• 제14권5호
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• pp.932-937
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• 2004

In situ extraction by organic solvent was studied in order to improve the recovery yield of hydrocarbon from the culture of Botryococcus braunii, a green colonial microalga. When the solvent mixture of octanol as an extractive solvent and n-octane as a biocompatible solvent was added to a two-phase column, the algal growth was seriously inhibited, even at a low concentration of polar octanol. Therefore, a two-stage cell-recycle extraction process was proposed to improve the contact area between the organic phase and the aqueous phase. The hydrocarbon recovery with in situ cell-recycle extraction showed a three-fold increase (57% of cell) in yield over that with two-phase extraction. In addition, over 60% of the hydrocarbon could be recovered without serious cell damage by downstream separation when this process was applied to the culture broth after batch fermentation.

• 한국연소학회:학술대회논문집
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• 한국연소학회 2007년도 제34회 KOSCO SYMPOSIUM 논문집
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• pp.63-68
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• 2007

An experimental study was carried out to investigate gasification process of wood sawdust in the 1-dimensional downdraft fixed bed gasifier. The preheated air which was used oxidizer and steam were used as a gasifying agent. The downdraft fixed bed gasifier obtains more amount of hydrogen and methane by increasing residence time of supplied air. The operating parameters, the supplied air temperature and steam were used. The oxidizer temperature was varied from 500K to 620K and vapor was added. The gasification process was monitored by measuring temperature at three position near the biomass using R-type thermocouples and the syngas composition was analyzed by gas chromatograph. We get the sample gas at the end of gasifier and it was eonugh time to finishing the chemical reaction. Finally, the amount of hydrogen and methane were increased widely as increasing the oxidizer temperature and adding steam.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.471-481
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• 2005

Heavy crudes (bitumen) are extremely viscous and contain high concentrations of asphaltene, resins, nitrogen and sulfur containing heteroaromatics and several metals, particularly nickel and vanadium. These properties of heavy crude oil present serious operational problems in heavy oil production and downstream processing. There are vast deposits of heavy crude oils in many parts of the world. In fact, these reserves are estimated at more than seven times the known remaining reserves of conventional crude oils. It has been proven that reserves of conventional crude oil are being depleted, thus there is a growing interest in the utilization of these vast resources of unconventional oils to produce refined fuels and petrochemicals by upgrading. Presently, the methods used for reducing viscosity and upgradation is cost intensive, less selective and environmentally reactive. Biological processing of heavy crudes may provide an ecofriendly alternative or complementary process with less severe process conditions and higher selectivity to specific reactions to upgrade heavy crude oil. This review describes the prospects and strengths of biological processes for upgrading of heavy crude oil.

• 한국기계가공학회지
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• 제11권4호
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• pp.62-66
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• 2012

The objective of this study is to simulate flow coefficient and flow characteristics such as velocity and pressure distribution for butterfly valve. Butterfly valves used in this study are 65A, 80A and 100A, in size, and of which the opening angle is varied. The flow coefficient, Kv, increases as the disc opening and valve size are increase. When using flow coefficient meanwhile specific curve of flow rate is also determined. The flow velocity between disc and seat increase as the disc opening decrease. The re-circulating zone is also observed in downstream behind disc.