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Cryopreservation of mesenchymal stem cells derived from dental pulp: a systematic review

  • Sabrina Moreira Paes;Yasmine Mendes Pupo;Bruno Cavalini Cavenago;Thiago Fonseca-Silva;Carolina Carvalho de Oliveira Santos
    • Restorative Dentistry and Endodontics
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    • v.46 no.2
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    • pp.26.1-26.15
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    • 2021
  • Objectives: The aim of the present systematic review was to investigate the cryopreservation process of dental pulp mesenchymal stromal cells and whether cryopreservation is effective in promoting cell viability and recovery. Materials and Methods: This systematic review was developed in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) statement and the research question was determined using the population, exposure, comparison, and outcomes strategy. Electronic searches were conducted in the PubMed, Cochrane Library, Science Direct, LILACS, and SciELO databases and in the gray literature (dissertations and thesis databases and Google Scholar) for relevant articles published up to March 2019. Clinical trial studies performed with dental pulp of human permanent or primary teeth, containing concrete information regarding the cryopreservation stages, and with cryopreservation performed for a period of at least 1 week were included in this study. Results: The search strategy resulted in the retrieval of 185 publications. After the application of the eligibility criteria, 21 articles were selected for a qualitative analysis. Conclusions: The cryopreservation process must be carried out in 6 stages: tooth disinfection, pulp extraction, cell isolation, cell proliferation, cryopreservation, and thawing. In addition, it can be inferred that the use of dimethyl sulfoxide, programmable freezing, and storage in liquid nitrogen are associated with a high rate of cell viability after thawing and a high rate of cell proliferation in both primary and permanent teeth.

Effects of fission product doping on the structure, electronic structure, mechanical and thermodynamic properties of uranium monocarbide: A first-principles study

  • Ru-Ting Liang;Tao Bo;Wan-Qiu Yin;Chang-Ming Nie;Lei Zhang;Zhi-Fang Chai;Wei-Qun Shi
    • Nuclear Engineering and Technology
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    • v.55 no.7
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    • pp.2556-2566
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    • 2023
  • A first-principle approach within the framework of density functional theory was employed to study the effect of vacancy defects and fission products (FPs) doping on the mechanical, electronic, and thermodynamic properties of uranium monocarbide (UC). Firstly, the calculated vacancy formation energies confirm that the C vacancy is more stable than the U vacancy. The solution energies indicate that FPs prefer to occupying in U site rather than in C site. Zr, Mo, Th, and Pu atoms tend to directly replace U atom and dissolve into the UC lattice. Besides, the results of the mechanical properties show that U vacancy reduces the compressive and deformation resistance of UC while C vacancy has little effect. The doping of all FPs except He has a repairing effect on the mechanical properties of U1-xC. In addition, significant modifications are observed in the phonon dispersion curves and partial phonon density of states (PhDOS) of UC1-x, ZrxU1-xC, MoxU1-xC, and RhxU1-xC, including narrow frequency gaps and overlapping phonon modes, which increase the phonon scattering and lead to deterioration of thermal expansion coefficient (αV) and heat capacity (Cp) of UC predicted by the quasi harmonic approximation (QHA) method.

Effectiveness and safety of rotary and reciprocating kinematics for retreatment of curved root canals: a systematic review of in vitro studies

  • Lucas Pinho Simoes;Alexandre Henrique dos Reis-Prado;Carlos Roberto Emerenciano Bueno;Ana Cecília Diniz Viana ;Marco Antonio Hungaro Duarte ;Luciano Tavares Angelo Cintra;Cleidiel Aparecido Araujo Lemos;Francine Benetti
    • Restorative Dentistry and Endodontics
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    • v.47 no.2
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    • pp.22.1-22.18
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    • 2022
  • Objectives: This systematic review (register-osf.io/wg7ba) compared the efficacy and safety of rotary and reciprocating kinematics in the removal of filling material from curved root canals. Materials and Methods: Only in vitro studies evaluating both kinematics during retreatment were included. A systematic search (PubMed/MEDLINE, Scopus, and other databases, until January 2021), data extraction, and risk of bias analysis (Joanna Briggs Institute checklist) were performed. Efficacy in filling removal was the primary outcome. Results: The search resulted in 2,795 studies, of which 15 were included. Efficacy was measured in terms of the remaining filling material and the time required for this. Nine studies evaluated filling material removal, of which 7 found no significant differences between rotary and reciprocating kinematics. Regarding the time for filling removal, 5 studies showed no difference between both kinematics, 2 studies showed faster results with rotary systems, and other 2 showed the opposite. No significant differences were found in apical transportation, centering ability, instrument failure, dentin removed and extruded debris. A low risk of bias was observed. Conclusions: This review suggests that the choice of rotary or reciprocating kinematics does not influence the efficacy of filling removal from curved root canals. Further studies are needed to compare the kinematics safety in curved root canals.

Pain management in periodontal therapy using local anesthetics and other drugs: an integrative review

  • Eduarda Cristina Santos;Daniela Huller;Sabrina Brigola;Marceli Dias Ferreira;Marcia Thais Pochapski;Fabio Andre dos Santos
    • Journal of Dental Anesthesia and Pain Medicine
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    • v.23 no.5
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    • pp.245-256
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    • 2023
  • Background: Surgical and non-surgical periodontal procedures often lead to postoperative pain. Clinicians use pharmacological methods such as anesthetics, anti-inflammatory drugs, and analgesics for relief. However, the multitude of options makes it challenging to select the best approach for routine dental care. Objective: This review aimed to describe previous studies regarding the pharmacological management used for pain control during periodontal procedures as well as factors that may interfere with patients' perception of pain. Methods: We included studies (period of 2000-2023, whose approach corresponded to the pharmacological protocols used for preoperative, trans-operative, and postoperative pain control in adult patients undergoing surgical and non-surgical periodontal therapy. Results: A total of 32 studies were included in the analysis, of which 17 (53%) were related to anesthetic methods and 15 (47%) were related to therapeutic protocols (anti-inflammatory/analgesic agents). These studies predominantly involved nonsurgical periodontal procedures. Studies have reported that factors related to age, type of procedure, and anxiety can influence pain perception; however, only seven of these studies evaluated anxiety. Conclusions: Numerous methods for pain control can be applied in periodontal therapy, which are accomplished through anesthetic methods and/or therapeutic protocols. Factors such as anxiety, age, and type of procedure are related to pain perception in patients. Thus, it is the responsibility of dentists to evaluate each clinical situation and define the best protocol to follow based on the literature.

Effects of a relined fiberglass post with conventional and self-adhesive resin cement

  • Wilton Lima dos Santos Junior;Marina Rodrigues Santi;Rodrigo Barros Esteves Lins;Luis Roberto Marcondes Martins
    • Restorative Dentistry and Endodontics
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    • v.49 no.2
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    • pp.18.1-18.13
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    • 2024
  • Objectives: This study was conducted to evaluate the mechanical properties of relined and non-relined fiberglass posts when cemented to root canal dentin using a conventional dual-cure resin cement or a self-adhesive resin cement. Materials and Methods: Two types of resin cements were utilized: conventional and self-adhesive. Additionally, 2 cementation protocols were employed, involving relined and non-relined fiberglass posts. In total, 72 bovine incisors were cemented and subjected to push-out bond strength testing (n = 10) followed by failure mode analysis. The cross-sectional microhardness (n = 5) was assessed along the root canal, and interface analyses (n = 3) were conducted using scanning electron microscopy (SEM). Data from the push-out bond strength and cross-sectional microhardness tests were analyzed via 3-way analysis of variance and the Bonferroni post-hoc test (α= 0.05). Results: For non-relined fiberglass posts, conventional resin cement exhibited higher pushout bond strength than self-adhesive cement. Relined fiberglass posts yielded comparable results between the resin cements. Type II failure was the most common failure mode for both resin cements, regardless of cementation protocol. The use of relined fiberglass posts improved the cross-sectional microhardness values for both cements. SEM images revealed voids and bubbles in the incisors with non-relined fiberglass posts. Conclusions: Mechanical properties were impacted by the cementation protocol. Relined fiberglass posts presented the highest push-out bond strength and cross-sectional microhardness values, regardless of the resin cement used (conventional dual-cure or self-adhesive). Conversely, for non-relined fiberglass posts, the conventional dual-cure resin cement yielded superior results to the self-adhesive resin cement.

B-spline polynomials models for analyzing growth patterns of Guzerat young bulls in field performance tests

  • Ricardo Costa Sousa;Fernando dos Santos Magaco;Daiane Cristina Becker Scalez;Jose Elivalto Guimaraes Campelo;Clelia Soares de Assis;Idalmo Garcia Pereira
    • Animal Bioscience
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    • v.37 no.5
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    • pp.817-825
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    • 2024
  • Objective: The aim of this study was to identify suitable polynomial regression for modeling the average growth trajectory and to estimate the relative development of the rib eye area, scrotal circumference, and morphometric measurements of Guzerat young bulls. Methods: A total of 45 recently weaned males, aged 325.8±28.0 days and weighing 219.9±38.05 kg, were evaluated. The animals were kept on Brachiaria brizantha pastures, received multiple supplementations, and were managed under uniform conditions for 294 days, with evaluations conducted every 56 days. The average growth trajectory was adjusted using ordinary polynomials, Legendre polynomials, and quadratic B-splines. The coefficient of determination, mean absolute deviation, mean square error, the value of the restricted likelihood function, Akaike information criteria, and consistent Akaike information criteria were applied to assess the quality of the fits. For the study of allometric growth, the power model was applied. Results: Ordinary polynomial and Legendre polynomial models of the fifth order provided the best fits. B-splines yielded the best fits in comparing models with the same number of parameters. Based on the restricted likelihood function, Akaike's information criterion, and consistent Akaike's information criterion, the B-splines model with six intervals described the growth trajectory of evaluated animals more smoothly and consistently. In the study of allometric growth, the evaluated traits exhibited negative heterogeneity (b<1) relative to the animals' weight (p<0.01), indicating the precocity of Guzerat cattle for weight gain on pasture. Conclusion: Complementary studies of growth trajectory and allometry can help identify when an animal's weight changes and thus assist in decision-making regarding management practices, nutritional requirements, and genetic selection strategies to optimize growth and animal performance.

A Digital Forensic Framework Design for Joined Heterogeneous Cloud Computing Environment

  • Zayyanu Umar;Deborah U. Ebem;Francis S. Bakpo;Modesta Ezema
    • International Journal of Computer Science & Network Security
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    • v.24 no.6
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    • pp.207-215
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    • 2024
  • Cloud computing is now used by most companies, business centres and academic institutions to embrace new computer technology. Cloud Service Providers (CSPs) are limited to certain services, missing some of the assets requested by their customers, it means that different clouds need to interconnect to share resources and interoperate between them. The clouds may be interconnected in different characteristics and systems, and the network may be vulnerable to volatility or interference. While information technology and cloud computing are also advancing to accommodate the growing worldwide application, criminals use cyberspace to perform cybercrimes. Cloud services deployment is becoming highly prone to threats and intrusions. The unauthorised access or destruction of records yields significant catastrophic losses to organisations or agencies. Human intervention and Physical devices are not enough for protection and monitoring of cloud services; therefore, there is a need for more efficient design for cyber defence that is adaptable, flexible, robust and able to detect dangerous cybercrime such as a Denial of Service (DOS) and Distributed Denial of Service (DDOS) in heterogeneous cloud computing platforms and make essential real-time decisions for forensic investigation. This paper aims to develop a framework for digital forensic for the detection of cybercrime in a joined heterogeneous cloud setup. We developed a Digital Forensics model in this paper that can function in heterogeneous joint clouds. We used Unified Modeling Language (UML) specifically activity diagram in designing the proposed framework, then for deployment, we used an architectural modelling system in developing a framework. We developed an activity diagram that can accommodate the variability and complexities of the clouds when handling inter-cloud resources.

The Regional Distribution of Ssireum(Traditional Wrestling) in South and North Korea (남북한 씨름의 지역적 분포)

  • Kwak, Nak-hyun
    • (The)Study of the Eastern Classic
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    • no.72
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    • pp.299-327
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    • 2018
  • The objective of this study is to examine the regional distribution of Ssireum(Traditional Wrestling) of South and North Korea in the Japanese colonial era. The conclusions of this study are as follows. First, the "Joseon ui hyangto orak(Folk play in Joseon)" showed the record of performing 272 times of Ssireum in 226 regions of the whole nation. Second, the Ssireum of South Korea could be divided into five regions. Seoul/Gyeonggi-do performed Ssireum the most in Dano, Baekjung, and Chuseok while Chungcheong-do performed Ssireum in Baekjung and Chuseok. Jeolla-do and Gyeongsang-do performed Ssireum in Chuseok while Gangwon-do performed Ssireum in Dano and Chuseok. Third, the Ssireum of North Korea could be divided into three regions. All the Hwanghae-do, Pyeongan-do, and Hamgyeong-do performed Ssireum the most in Dano. Fourth, as the period when Ssireum was held the most in the whole nation, Dano, Baekjung, and Chuseok could be pointed out. Ssireum has the characteristics of large-scale play between village communities or regions, instead of individual game. Thus, the Ssireum that was played as a sport event under certain rules for a long time was settled down as a folk play of regional festivals such as Dano, Baekjung, and Chuseok. Fifth, as a folk play and a representative play of seasonal customs, Ssireum was distributed in the whole nation and handed down till today under the regional deviation of South and North Korea. Sixth, the unidentified regions of South Korea that did not perform Ssireum were six places including five dos such as Gapyeong Gyeonggi-do, Boseong Jeollanam-do, Jeju-do, Gunwi and Cheongsong Gyeongsanbuk-do, and Inje Gangwon-do. The regions of North Korea were six places including three dos such as Pyeongyang, Yangdeok, Gangdong, and Gaecheon of Pyeongannam-do, Bakcheon Pyeonganbuk-do, and Dancheon Hamgyeongnam-do. Total 12 places in eight regions were included. Seventh, the number of total items of play names presented in the "Joseon ui hyangto orak(Folk play in Joseon)" was about 6,400 types. Out of them, about 1,300 types were the items including how to play while about 5,100 types were the items presenting the play names only without explanations. Especially, in case of Ssireum, the periods of the lunar calendar were only specified in each region. Unfortunately, it was not possible to check the contents about the actual performance methods and types of Ssireum as they were omitted.

Performance Analysis of TCAM-based Jumping Window Algorithm for Snort 2.9.0 (Snort 2.9.0 환경을 위한 TCAM 기반 점핑 윈도우 알고리즘의 성능 분석)

  • Lee, Sung-Yun;Ryu, Ki-Yeol
    • Journal of Internet Computing and Services
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    • v.13 no.2
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    • pp.41-49
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    • 2012
  • Wireless network support and extended mobile network environment with exponential growth of smart phone users allow us to utilize the network anytime or anywhere. Malicious attacks such as distributed DOS, internet worm, e-mail virus and so on through high-speed networks increase and the number of patterns is dramatically increasing accordingly by increasing network traffic due to this internet technology development. To detect the patterns in intrusion detection systems, an existing research proposed an efficient algorithm called the jumping window algorithm and analyzed approximately 2,000 patterns in Snort 2.1.0, the most famous intrusion detection system. using the algorithm. However, it is inappropriate from the number of TCAM lookups and TCAM memory efficiency to use the result proposed in the research in current environment (Snort 2.9.0) that has longer patterns and a lot of patterns because the jumping window algorithm is affected by the number of patterns and pattern length. In this paper, we simulate the number of TCAM lookups and the required TCAM size in the jumping window with approximately 8,100 patterns from Snort-2.9.0 rules, and then analyse the simulation result. While Snort 2.1.0 requires 16-byte window and 9Mb TCAM size to show the most effective performance as proposed in the previous research, in this paper we suggest 16-byte window and 4 18Mb-TCAMs which are cascaded in Snort 2.9.0 environment.

How image-processing parameters can influence the assessment of dental materials using micro-CT

  • Torres, Fernanda Ferrari Esteves;Jacobs, Reinhilde;EzEldeen, Mostafa;de Faria-Vasconcelos, Karla;Guerreiro-Tanomaru, Juliane Maria;dos Santos, Bernardo Camargo;Tanomaru-Filho, Mario
    • Imaging Science in Dentistry
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    • v.50 no.2
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    • pp.161-168
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    • 2020
  • Purpose: The aim of this study was to evaluate the influence of voxel size and different post-processing algorithms on the analysis of dental materials using micro-computed tomography (micro-CT). Materials and Methods: Root-end cavities were prepared in extracted maxillary premolars, filled with mineral trioxide aggregate (MTA), Biodentine, and Intermediate Restorative Material (IRM), and scanned using micro-CT. The volume and porosity of materials were evaluated and compared using voxel sizes of 5, 10, and 20 ㎛, as well as different software tools(post-processing algorithms). The CTAn or MeVisLab/Materialise 3-matic software package was used to perform volume and morphological analyses, and the CTAn or MeVisLab/Amira software was used to evaluate porosity. Data were analyzed using 1-way ANOVA and the Tukey test(P<0.05). Results: Using MeVisLab/Materialise 3-matic, a consistent tendency was observed for volume to increase at larger voxel sizes. CTAn showed higher volumes for MTA and IRM at 20 ㎛. Using CTAn, porosity values decreased as voxel size increased, with statistically significant differences for all materials. MeVisLab/Amira showed a difference for MTA and IRM at 5 ㎛, and for Biodentine at 20 ㎛. Significant differences in volume and porosity were observed in all software packages for Biodentine across all voxel sizes. Conclusion: Some differences in volume and porosity were found according to voxel size, image-processing software, and the radiopacity of the material. Consistent protocols are needed for research evaluating dental materials.