• Title/Summary/Keyword: Dongbu

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Identification of a Third Haplotype of the Sequence Linked to the Restorer-of-fertility (Rf) Gene and Its Implications for Male-Sterility Phenotypes in Peppers (Capsicum annuum L.)

  • Min, Woong-ki;Lim, Heerae;Lee, Young-Pyo;Sung, Soon-Kee;Kim, Byung-Dong;Kim, Sunggil
    • Molecules and Cells
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    • v.25 no.1
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    • pp.20-29
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    • 2008
  • Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.

Development Methodology of Enterprise Applications based on the ISIS (ISIS 기반의 엔터프라이즈 어플리케이션 개발방법)

  • Jo, Eun-Hwan;Lee, Jong-Kook;Lee, Min-Soo;Chang, Young-Chul;Lee, Bong
    • 한국IT서비스학회:학술대회논문집
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    • 2010.05a
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    • pp.529-535
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    • 2010
  • 오늘날의 엔터프라이즈 IT 환경은 비즈니스 요구사항이 빠르게 변하면서 기존 시스템들의 민첩한 대응이 어렵고, 비즈니스 프로세스가 점점 복잡해지면서 시스템 개발 및 제어가 힘든 상황이다. 이 때문에 관리비용은 점점 증가하고 시스템의 수명주기가 단축되면서 전체적인 개발생산성이 낮아지고 있다. 최근 이와 같은 소프트웨어 개발문제를 극복하기 위해서 XP(eXtreme Programming)와 SCRUM 같은 애자일(agile) 방법론이 등장하게 되었고, 점차 대규모의 엔터프라이즈 어플리케이션 개발에 적용할 필요성이 대두되고 있다. 따라서 본 논문에서는 엔터프라이즈 어플리케이션의 개발생산성을 향상시키고 수명주기를 연장시키는데 효과적인 ISIS(Integrated System of Independent Sub-systems) 개발방법을 제안하고자 한다.

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Optimal Hot Water Extraction Conditions of Mixed Herbs Extract Mixture Using Response Surface Methodology

  • Park, Tae-Young;Oh, Junseok;Hong, Jae-Heoi;Hong, Seong-Eun;Hong, Seong-Min;Oh, Hyeon-Min;Park, Gyeong-Su;Jeong, Hee Gyeong;Kim, Kyung Je;Jin, Seong Woo;Koh, Young Woo;Im, Seung Bin;Ha, Neul-I;Seo, Kyoungsun
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.04a
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    • pp.106-106
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    • 2019
  • Human needs energy to maintain metabolisms, and these energy sources were uptake foods or nutritions. The most effective source was known for glucose among the nutrients, furthermore the glucose is an important source of energy for blood cells and control brain maintenences cells. But as food is plentiful and eating habits become more westernized, fast food and irregular meal times by works. Nowadays, diabetes were rapidly increased by malnutriton and obesity. Diabetes was the sixth highest on the list of causes of death in Korea, released by the Statistics Korea in 2015, which is considered a serious social problem for adult diseases. Therefore, this study aims to establish the optimal hot water extraction conditions of mixed herbs extract mixture compounds that are effective in diabetes. The independent factors were extraction temperature (X1: $40-120^{\circ}C$), extraction time (X2: 2-10 hrs.), and the ratio of water to sample (X3: 40-200 mg/mL). Their effects were assessed on dependent variables of the extract properties, which included soluble solid contents, Brix of sample extract, total polyphenols content, total flavonoids content and DPPH Radical scavenging activity. As a result, the content of total polyphenol content was the highest in No.12(6 hrs, $120^{\circ}C$, 67 mg/mL) and the highest total flavonoid contents was found in No.16(6 hrs, $80^{\circ}C$, 40 mg/mL). DPPH Radical scavenging activity showed the highest activity in No.7(8 hrs, $100^{\circ}C$, 100 mg/mL).

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Mechanical and Sensory Characteristics of Dongbu-Mook (Cowpea Starch Gel) by the addition of Soybean Oil and Salt (대두유와 소금의 첨가에 따른 동부 묵의 기계적 텍스쳐 및 관능적 특성)

  • 구미영;이재경;김기숙
    • Korean journal of food and cookery science
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    • v.18 no.3
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    • pp.275-279
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    • 2002
  • This study was carried out to investigate the effects of soybean oil and salt on the mechanical texture and sensory characteristics of Dongbu-Mook (Cowpea Starch Gel). As the amount of the soybean oil was increased, the hardness and chewiness of the Mook were decreased. But there were no significant difference in the cohesiveness and springiness. While the Mook samples made with commercial Dongbu-Mook flour didn't show a significant difference, those made with lab-manufactured Dongbu-Mook flour without salt showed higher hardness, chewiness than the ones with salt. The Mook made of commercial Dongbu-Mook flour was found to be higher in the hardness and lower in the cohesiveness than the one made of lab-manufactured Dongbu-Mook flour. In sensory evaluation, as the amount of soybean oil was increased, all the samples showed higher yellowness and greasiness, and lower clarity, hardness and chewiness. For the samples with salt, the yellowness was high, and the hardness was low. The Mook samples made with lab-manufactured Dongbu-Mook flour had low chewiness. There was no significant difference in the clarity and greasiness. Overall, the Mook samples made with commercial Dongbu-Mook flour showed the best quality, when soybean oil was not added and salt was added. For the Mook made with lab-manufactured Dongbu-Mook flour, the sample with 2% oil and salt was the best.