• 제목/요약/키워드: Dongbu

검색결과 376건 처리시간 0.031초

Identification of a Third Haplotype of the Sequence Linked to the Restorer-of-fertility (Rf) Gene and Its Implications for Male-Sterility Phenotypes in Peppers (Capsicum annuum L.)

  • Min, Woong-ki;Lim, Heerae;Lee, Young-Pyo;Sung, Soon-Kee;Kim, Byung-Dong;Kim, Sunggil
    • Molecules and Cells
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    • 제25권1호
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    • pp.20-29
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    • 2008
  • Cytoplasmic male sterility (CMS), one of the most important traits in crop breeding, has been used for commercial seed production by $F_1$ hybrid cultivars of pepper (Capsicum annuum L.). To develop reliable molecular markers for allelic selection of the Restorer-of-fertility (Rf) gene, which is known to be a major determinant of pollen fertility restoration in peppers, a sequence of approximately 10 kb flanking an RAPD fragment closely linked to the Rf locus was obtained by genome walking. A homology search revealed that this sequence contained an LTR retrotransposon and a non-LTR LINE-like retrotransposon. Sequencing of this Rf-linked region to search for polymorphisms between a dominant and recessive allele revealed 98% nucleotide sequence identity between them. A third polymorphic haplotype of the Rf-linked sequence, which has 94-96% nucleotide sequence identity with the two previously isolated haplotypes, was identified among a large number of breeding lines. Utilizing polymorphic sequences in the haplotypes, PCR markers were developed for selection of particular haplotypes and used to examine the distribution of the haplotypes in diverse breeding lines, cultivars, and C. annuum germplasms. Surprisingly, the third haplotype was the predominant type in C. annuum germplasms, while its frequency in $F_1$ hybrid cultivars was relatively low. Meanwhile, analysis of breeding lines whose Rf allele genotypes and male-sterility phenotypes were already known revealed that the third haplotype was mainly present in exotic breeding lines that cause unstable male-sterility when combined with sterile cytoplasms.

ISIS 기반의 엔터프라이즈 어플리케이션 개발방법 (Development Methodology of Enterprise Applications based on the ISIS)

  • 조은환;;이민수;장영철;이봉
    • 한국IT서비스학회:학술대회논문집
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    • 한국IT서비스학회 2010년도 춘계학술대회
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    • pp.529-535
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    • 2010
  • 오늘날의 엔터프라이즈 IT 환경은 비즈니스 요구사항이 빠르게 변하면서 기존 시스템들의 민첩한 대응이 어렵고, 비즈니스 프로세스가 점점 복잡해지면서 시스템 개발 및 제어가 힘든 상황이다. 이 때문에 관리비용은 점점 증가하고 시스템의 수명주기가 단축되면서 전체적인 개발생산성이 낮아지고 있다. 최근 이와 같은 소프트웨어 개발문제를 극복하기 위해서 XP(eXtreme Programming)와 SCRUM 같은 애자일(agile) 방법론이 등장하게 되었고, 점차 대규모의 엔터프라이즈 어플리케이션 개발에 적용할 필요성이 대두되고 있다. 따라서 본 논문에서는 엔터프라이즈 어플리케이션의 개발생산성을 향상시키고 수명주기를 연장시키는데 효과적인 ISIS(Integrated System of Independent Sub-systems) 개발방법을 제안하고자 한다.

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Optimal Hot Water Extraction Conditions of Mixed Herbs Extract Mixture Using Response Surface Methodology

  • Park, Tae-Young;Oh, Junseok;Hong, Jae-Heoi;Hong, Seong-Eun;Hong, Seong-Min;Oh, Hyeon-Min;Park, Gyeong-Su;Jeong, Hee Gyeong;Kim, Kyung Je;Jin, Seong Woo;Koh, Young Woo;Im, Seung Bin;Ha, Neul-I;Seo, Kyoungsun
    • 한국자원식물학회:학술대회논문집
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    • 한국자원식물학회 2019년도 춘계학술대회
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    • pp.106-106
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    • 2019
  • Human needs energy to maintain metabolisms, and these energy sources were uptake foods or nutritions. The most effective source was known for glucose among the nutrients, furthermore the glucose is an important source of energy for blood cells and control brain maintenences cells. But as food is plentiful and eating habits become more westernized, fast food and irregular meal times by works. Nowadays, diabetes were rapidly increased by malnutriton and obesity. Diabetes was the sixth highest on the list of causes of death in Korea, released by the Statistics Korea in 2015, which is considered a serious social problem for adult diseases. Therefore, this study aims to establish the optimal hot water extraction conditions of mixed herbs extract mixture compounds that are effective in diabetes. The independent factors were extraction temperature (X1: $40-120^{\circ}C$), extraction time (X2: 2-10 hrs.), and the ratio of water to sample (X3: 40-200 mg/mL). Their effects were assessed on dependent variables of the extract properties, which included soluble solid contents, Brix of sample extract, total polyphenols content, total flavonoids content and DPPH Radical scavenging activity. As a result, the content of total polyphenol content was the highest in No.12(6 hrs, $120^{\circ}C$, 67 mg/mL) and the highest total flavonoid contents was found in No.16(6 hrs, $80^{\circ}C$, 40 mg/mL). DPPH Radical scavenging activity showed the highest activity in No.7(8 hrs, $100^{\circ}C$, 100 mg/mL).

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대두유와 소금의 첨가에 따른 동부 묵의 기계적 텍스쳐 및 관능적 특성 (Mechanical and Sensory Characteristics of Dongbu-Mook (Cowpea Starch Gel) by the addition of Soybean Oil and Salt)

  • 구미영;이재경;김기숙
    • 한국식품조리과학회지
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    • 제18권3호
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    • pp.275-279
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    • 2002
  • 동부 묵가루 제조 방법과 대두유 첨가량과 소금의 첨가 유무를 달리한 묵을 제조하여 기계적인 texture특성을 측정하고, 관능검사를 실시하여 대두유와 소금의 첨가가 묵의 품질특성에 미치는 영향을 검토한 결과를 요약하면 다음과 같다. 1. 소금의 첨가유무에 관계없이 대두유의 첨가량이 많을수록 hardness, chewiness는 전반적으로 감소하는 경향이었으나, cohesiveness, springiness는 대두유 첨가량에 따라 큰 차이가 없었다. 시판 묵가루를 이용하여 만든 묵이 실험실 제조 묵가루를 이용한 묵보다 hardness는 높았고, cohesiveness는 낮았으며, springiness, chewiness는 큰 차이를 나타내지 않았다. 2. 관능검사 결과 대두유를 첨가할수록 노르스름하고, 불투명하며, 느끼하고, 부드럽게 평가되었으나, 전반적인 바람직성에서는 바람직하지 않은것으로 평가되었다. 그러나 소금을 첨가하였을 경우 소금을 첨가하지 않은 시료보다 대두유 첨가량에 따른 차이가 적었으므로 묵 제조 시 대두유와 소금을 첨가하여 부드러우며, 기본적인 간을 나타내는 묵을 만들 수 있을 것으로 생각된다.