• Reproductive and Developmental Biology
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• 제30권3호
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• pp.163-167
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• 2006

The purpose of the present study was to establish culture conditions for the in vitro study of the neonatal piglet Sertoli cell. Isolation for the culture of Sertoli cell was established using collagenase and pancreatin digestion of testicular tissues. The effects of various culture media, fetal bovine serum(FBS), follicular stimulating hormone(FSH), epidermal growth factor(EGF) and insulin-transferrin-sodium selenite(ITS) on growth of neonatal piglet Sertoli cells were investigated. The mitogenic effects of Dulbecco's modified Eagle's medium+Ham's F-12 medium was higher than other media used in this experiment. The addition of 1% FBS in cultures was necessary for attachment of Sertoli cell clusters. However, except FBS and EGF, FSH and ITS did not stimulate Sertoli cell proliferation. When Sertoli cells isolated from neonatal piglets were cultured in Dulbecco's modified Eagle's medium+Ham's F-12 medium supplemented with 1% FBS, FSH EGF and ITS, the yield and plating efficiency of Sertoli cells were largely increased. Confluency of Sertoli cells was reached as early as 4 days of culture. The method described here reduces or eliminates many of the drawbacks of the conventional procedures used to isolate and culture of Sertoli cells, thus providing a useful tool in studies of growth kinetics and regulation of cell proliferation in vitro.

• The International Journal of Costume Culture
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• 제12권2호
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• pp.202-209
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• 2009

Hakka is a very special sub-ethnic group in China, which was formed during several migrations in Chinese war history. Today the majority of Hakka people, living in the boundary between Jiangxi, Guangdong, and Fujian province, still keep a quite old custom and culture. The bell hat for children is a unique and splendid illustration. We have imposed researches and studies in local area a lot and interviewed the local crafts-women to get to know more about bell hat. We are trying to describe the decorations on the bell hat, silver items and embroidery respectively, and then give an overall analysis of Chinese auspicious culture that the motifs of decorations have contained.

• Journal of Plant Biotechnology
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• 제34권4호
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• pp.355-361
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• 2007

Cotyledons of perilla6 were cultured on MS medium containing 0.5 mg/l NAA and 0.5 mg/l BA for 7 weeks. The activity of perilla peroxidase was observed to increase following culture stages as assessed by peroxidase assay. A peroxidase (POD) was purified from perilla tissue cultured on MS medium for 7 weeks. The peroxidase was purified using ion exchange and gel nitration chromatography. The perilla peroxidase had a molecular mass of 30 kDa by SDS-PAGE. We showed that the N-terminal amino acid sequence of this protein shared 67% identity with the tea peroxidase. As indicated by SDS-PAGE, the banding pattern of the 30 kDa polypeptide present in total soluble protein from perilla tissue was increased following culture stages. Immunoblot analysis indicated that perilla peroxidase protein appeared after 3 weeks of perilla tissue culture, and continued to increase with extended duration of tissue culture for at least 7 weeks.

• 생명과학회지
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• 제16권3호
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• pp.459-463
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• 2006

경제적인 채소류 중 향신료뿐만 아니라 약용으로서 이용 가치가 많은 마늘은 영양번식성 작물로 virus에 감염되어 있으므로, 조직배양에 의하여 마늘의 virus-free한 개체의 생산을 목적으로, 배양부위의 상위에 따른 배양효과의 차이와 호르몬 조성과 당의 농도가 조직배양의 생육 분화에 미치는 영향을 조사하였다. 또한 신초원기 유도를 위한 배양조건을 검토하였으며, 배양체의 조직학적 관찰을 행하였다. 치상부위 별로 보통엽 중하부조직이 배지조성에는 NAA 0.02 + BAP 1.0 mg/l 첨가배지가 발육과 분화에 다소 양호하였다. Shoot 분화에는 sucrose 40 g 첨가구에서 BAP의 증가에 따라 shoot형성이 다소 양호하였으며 NAA 0.02 + BAP 0.2 mg/l 첨가구에서는 가근이 유도되었다. 액체 회전 배양에서 신초원기라고 판단되는 조직이 유도되었다.

• 한국멀티미디어학회논문지
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• 제25권10호
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• pp.1457-1467
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• 2022

The relationship between film and culture is established through a shared focus on people's lives. Culture changes at different times, which makes a dynamic and complex relationship between film and culture. Understanding this relationship helps to provide better support for current filmmaking. The purpose of this study is to provide insights into the macro issue of film and culture by exploring the relationship between film theme and culture concept. To this end, we used a case study to explore the connection between themes and culture concepts in Disney's Mulan (1998, 2020) through literature and analyses of culture concepts (Chinese "imagery" and Postmodernism). The study concludes that, "Culture assemblage" and "Vision is king" have become cultural trends that influence the creation of film theme and style to a certain extent; how to balance the relationship between the core idea of film theme and the external forms is an important issue in current filmmaking. The article contributes to promote thinking about culture in film creation, and its research perspective on different cultures and histories is innovative.

• 한국의상디자인학회지
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• 제7권2호
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• pp.171-176
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• 2005

본 연구는 역사문화의 입장에서 중국 복식문화의 원류에 관해 연구한 것으로 무속과 예약문화가 그 형성에 영향을 미친것을 밝혔다. 중국복식문화는 고대 주술문화에서 중요한 역할을 했을 뿐 아니라 상당한 비중을 차지하였다. 중국 복식문화의 성숙기와 서주때의 중국 예약문화의 형성은 매우 밀접한 상관이 있다. 예약문화의 표현에서 계급성을 내용으로 하고 형 식 성을 특징으로 하는 체제에서 중요한 작용을 하였다 동시 에 복식문화 자체도 이 시 기 에 아주 풍요롭게 발전하여 중국 문화의 견고한 기초를 확립하였다.

• 한국수산과학회지
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• 제50권4호
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• pp.378-387
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• 2017

This study was performed to isolate bacteria that could control the nitrite levels in a biofloc technology (BFT) culture tank. Nitrite-eliminating bacteria were isolated from a BFT culture tank rearing goldfish, and the isolated bacterium exhibiting the most potent nitrite eliminating ability was labeled as the "NOBSB1" strain. Sequencing the 16S rRNA revealed that NOBSB1 is a species in the genera Bosea. NOBSB1 had the following characteristics with regard to nitrite removal: (1) it removed nitrite by functioning heterotrophically in the presence of a carbon source (sugars); (2) it eliminated nitrite most effectively within a temperature range of $20-30^{\circ}C$, but its activity decreased at temperatures above $35^{\circ}C$ and below $20^{\circ}C$; (3) it had optimum nitrite removal ability within a pH range of 6.0-8.0; (4) it removed nitrite more effectively under hypoxic than aerobic conditions. NOBSB1 inoculation did not decrease ammonia or nitrate levels, but eliminated nitrite in a BFT culture tank rearing common carp (Cyprinus carpio). After inoculating the NOBSB1 strain in a BFT culture tank, NOBSB1 controlled and sufficiently reduced the nitrite concentration in the tank.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.253-256
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• 2003

In batch culture of A. pullulans HP-2001, maximal production of pullulan was 57.90 g/l at 96 hr. The maximal production of pullulan with 76.55 g/l occurred when substituted solution was 10% (w/v) sucrose, 0.25% (w/v) yeast extract and mineral salts. The maximal production of pullulan was 75.00 g/l with a dilution rate of $0.015\;h^{-1}$. Productivity of pullulan in the continuous culture fed with the fresh medium was higher than that fed with the fresh medium without nitrogen source.

• KIEAE Journal
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• 제4권1호
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• pp.33-38
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• 2004

There are many attempts to make more comfortable amenity in city districts and streets to provide a soundscape design. And the sound which reflects the culture and peculiarity of characterized the street must be preserved. Thus these attempts are recognized as another noise control to give the suitable environmental sound, not to reduce noise level. This study aims to analyze the behavior of the pedestrian and sound environment of the street sound, by measuring the sound level for the Insa-Dong Traditional Culture street. As the results, it is advisable to use the natural water sound dominating the district and to provide the staying spot to watch the event to be held. The results of this study could be used as fundamental data to recover the image of Insa-Dong Traditional Culture street to consider design concept of soundscape.

• The International Journal of Costume Culture
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• 제13권2호
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• pp.82-87
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• 2010

Frog or toad is an animal adoration of ancient ancestors in ethnic minorities in South China, in Li tribe tradition, frog is not only a symbol of avoiding evil and the expression of maternal love, but also the decoration of missing the ancestors. This article has discussed the origin of frog pattern, summarized the frog pattern's forms in Li brocade. At last also discussed the common frog pattern in Li tribe.