• BMB Reports
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• 제40권2호
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• pp.239-246
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• 2007

Riboflavin synthase from Escherichia coli is a homotrimer of 23.4 kDa subunits and catalyzes the formation of one molecule each of riboflavin and 5-amino-6-ribitylamino- 2,4(1H,3H)-pyrimidinedione by the transfer of a 4-carbon moiety between two molecules of the substrate, 6,7- dimethyl-8-ribityllumazine. Each subunit comprises two closely similar folding domains. Recombinant expression of the N-terminal domain is known to provide a $C_2$-symmetric homodimer. In this study, the binding properties of wild type as well as two mutated proteins of N-terminal domain of riboflavin synthase with various ligands were tested. The replacement of the amino acid residue A43, located in the second shell of riboflavin synthase active center, in the recombinant N-terminal domain dimer reduces the affinity for 6,7-dimethyl-8-ribityllumazine. The mutation of the amino acid residue C48 forming part of activity cavity of the enzyme causes significant $^{19}F$ NMR chemical shift modulation of trifluoromethyl derivatives of 6,7-dimethyl-8-ribityllumazine in complex with the protein, while substitution of A43 results in smaller chemical shift changes.

• 전력전자학회:학술대회논문집
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• 전력전자학회 1999년도 전력전자학술대회 논문집
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• pp.618-621
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• 1999

With the advent of the high-speed microprocessor and DSP, the possibility of executing a control strategy in digital domain has become a reality. By the use of the DSP and microprocessor controller, many high power drive system may be enhanced resulting in the improved robustness to EMI, the ability to communicate the operating conditions and the ease of adjusting the control parameters. But, the digital controller using DSP or microprocessor is not applied in the high frequency switching power supplies, especially full bridge DC/DC converter. So, this paper presents the method and realization of designing a digital-to-phase shift PWM circuit for full digital controlled full bridge DC/DC converter with zero voltage switching. The operating principles, simulation and experimental results will be presented.

• 한국전산구조공학회:학술대회논문집
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• 한국전산구조공학회 2007년도 정기 학술대회 논문집
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• pp.21-26
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• 2007

This paper presents an impedance-based structural health monitoring (SHM) technique considering temperature effects. The temperature variation results in a significant impedance variation, particularly both horizontal and vertical shifts in the frequency domain, which may lead to erroneous diagnostic results of real structures. A new damage detection strategy has been proposed based on the correlation coefficient (CC) between the reference impedance data and a concurrent impedance data with an effective frequency shift which is defined as the shift causing the maximum correlation. The proposed technique was applied to a lab-sized steel truss bridge member under the temperature varying environment. From an experimental study, it has been demonstrated that a narrow cut inflicted artificially to the steel structure was successfully detected using the proposed SHM strategy.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2019년도 추계학술대회
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• pp.124-125
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• 2019

This paper proposes a novel control method to minimize conduction losses of dual active bridge (DAB) converters for on-board chargers of electric vehicles (EVs). In the control method, two variables are regulated, which are the phase-shift angle between the primary and secondary full bridges and the switching frequency. From time-domain analysis, an optimal phase-shift angle is derived to achieve the minimum RMS value of the transformer current. The proposed method was implemented for a 3.6-kW SiC-based prototype to validate its effectiveness. A high efficiency over 97.3% has been achieved for a wide output voltage range.

• Bulletin of the Korean Chemical Society
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• 제24권7호
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• pp.971-974
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• 2003

By utilizing singular value decomposition (SVD) and shift averaged Harr wavelet transform (WT) with a set of Daubechies wavelet coefficients (1/2, -1/2), a method that can simultaneously eliminate an unwanted large solvent peak and noise peaks from NMR data has been developed. Noise elimination was accomplished by shift-averaging the time domain NMR data after a large solvent peak was suppressed by SVD. The algorithms took advantage of the WT, giving excellent results for the noise elimination in the Gaussian type NMR spectral lines of NMR data pretreated with SVD, providing superb results in the adjustment of phase and magnitude of the spectrum. SVD and shift averaged Haar wavelet methods were quantitatively evaluated in terms of threshold values and signal to noise (S/N) ratio values.

• 한국군사과학기술학회지
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• 제24권4호
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• pp.382-391
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• 2021

Unsupervised domain adaptation often gives impressive solutions to handle domain shift of data. Most of current approaches assume that unlabeled target data to train is abundant. This assumption is not always true in practices. To tackle this issue, we propose a general solution to solve the domain gap minimization problem without any target data. Our method consists of two regularization steps. The first step is a pixel regularization by arbitrary style transfer. Recently, some methods bring style transfer algorithms to domain adaptation and domain generalization process. They use style transfer algorithms to remove texture bias in source domain data. We also use style transfer algorithms for removing texture bias, but our method depends on neither domain adaptation nor domain generalization paradigm. The second regularization step is a feature regularization by feature alignment. Adding a feature alignment loss term to the model loss, the model learns domain invariant representation more efficiently. We evaluate our regularization methods from several experiments both on small dataset and large dataset. From the experiments, we show that our model can learn domain invariant representation as much as unsupervised domain adaptation methods.

• 한국자기공명학회논문지
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• 제21권3호
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• pp.109-113
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• 2017

The hepatitis C virus (HCV) internal ribosome entry site (IRES) is an RNA structure located in the 5'-UTR of the HCV RNA genome. The HCV IRES consists of four domains I, II, III, and IV, where domains II - IV are recognized by 40S ribosomal subunit and the domain III is bound to eukaryotic initiation factor 3 (eIF3) for translation initiation. Here, we have characterized the tertiary interaction between an L-/K- rich peptide and the HCV IRES domain IV. To probe the peptide binding interface in RNA, we synthesized $^{13}C$- and $^{15}N$-double labeled RNA and the binding site was identified by using the chemical shift perturbation (CSP) NMR methods. Our results showed that the peptide binds to the upper stem of the IRES domain IV, indicating that the tertiary interaction between the IRES domain IV and the peptide would disrupt the initiation of translation of HCV mRNA by blocking the start codon exposure. This study will provide an insight into the new peptide-based anti-viral drug design targeting HCV IRES RNA.

• 한국자기공명학회논문지
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• 제26권3호
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• pp.34-39
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• 2022

The SH3 domain found within a variety of proteins is comprised of generally 60 residues, and participated in protein-protein interactions with proline-rich motifs. Cobll1 was identified as a distinct molecular marker associated with CML progression, and PACSIN2 was discovered a novel Cobll1 binding partner through direct interaction between a SH3 domain of PACSIN2 and three proline-rich motifs of Cobll1. To understand the structural basis of interactions between PACSIN2 and Cobll1, backbone assignments of PACSIN2 SH3 domain were performed. Furthermore, three proline-rich peptides of Cobll1 were titrated to ¹⁵N-labeled PACSIN2 SH3 domain in various ratios. Our chemical shift changes data and conserved SH3 sequence alignment will be helpful to analyze fundamental molecular basis related to the interaction between PACSIN2 and Cobll1.

• 한국통신학회논문지
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• 제28권9C호
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• pp.868-875
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• 2003

본 논문에서는 웨이블릿 변환 영역에서 기존의 다중해상도 움직임 예측 방법에 비해 보다 향상된 단화소 이동 감쇠를 이용한 다중해상도 움직임 예측 방법을 제안하였다. 웨이블릿 변환 영역에서 웨이블릿 계수들의 계층적 상관관계를 이용한 기존의 다중해상도 움직임 예측 방법(MRME)은 웨이블릿 변환시 수행되는 다운 샘플링 과정에서 발생되는 웨이블릿 계수들의 이동-변환 성질(shift-variant property)에 의해 정확한 움직임 예측을 수행할 수 없다는 단점이 있다. 따라서 이러한 문제점을 극복하기 위해 제안된 방법은 입력 영상에 대해서 2레벨 웨이블릿 변환을 수행한 후 저대역 신호인 S$_4$대역에 대해 3레벨 웨이블릿 변환을 수행하기 앞서 S$_4$대역에서의 단화소 이동된 신호를 제거하기 위한 방법으로 보간을 적용한다. 보간된 저 대역 신호 S$_4$대역에 대해서 1레벨 웨이블릿 변환을 수행한 후 최종적으로 3레벨 웨이블릿 변환된 저 대역 신호 S$_{8}$대역에 대해서 초기 움직임 벡터를 구한 다음 나머지 하위 레벨에 위치한 대역에 대해서 기존 다중해상도 움직임 예측 방법과 동일한 방법으로 움직임 예측을 수행함으로써 향상된 부호화 성능을 얻을 수 있었다. 실험 결과 제안한 방법은 기존 다중해상도 움직임 예측 방법과 웨이블릿 변환 영역에서 전역 탐색 방법과 비교해 PSNR면에서 약 1∼2dB정도 향상된 부호화 효율을 나타낼 뿐 아니라, 주관적 화질에서도 개선된 결과를 보였다.

• ETRI Journal
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• 제42권3호
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• pp.324-332
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• 2020

In this paper, a blind symbol timing offset (STO) estimation method is proposed for offset quadrature phase-shift keying (OQPSK) modulated signals, which also works for other linearly modulated signals (LMS) such as binary-PSK, QPSK, 𝜋/4-QPSK, and minimum-shift keying. There are various methods available for blind STO estimation of LMS; however, none work in the case of OQPSK modulated signals. The popular cyclic correlation method fails to estimate STO for OQPSK signals, as the offset present between the in-phase (I) and quadrature (Q) components causes the cyclic peak to disappear at the symbol rate frequency. In the proposed method, a set of close and approximate offsets is used to compensate the offset between the I and Q components of the received OQPSK signal. The STO in the time domain is represented as a phase in the cyclic frequency domain. The STO is therefore calculated by obtaining the phase of the cyclic peak at the symbol rate frequency. The method is validated through extensive theoretical study, simulation, and testbed implementation. The proposed estimation method exhibits robust performance in the presence of unknown carrier phase offset and frequency offset.