• 한국식물병리학회:학술대회논문집
• /
• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
• /
• pp.81.2-82
• /
• 2003

Plants have evolved along with pathogens, and they have developed sophisticated defense systems against specific microorganisms to survive. G-protons are considered one of the upstream signaling components working as a key for the defense signal transduction pathway. For activation and inactivation of G-protein, GTP-biding proteins are involved. GTP -binding proteins are found in all organisms. Small GTP-binding proteins, having masses of 21 to 30kD, belong to a superfamily, often named the Ras supefamily because the founding members are encoded by human Ras genes initially discovered as cellular homologs of the viral ras oncogene. Members of this supefamily share several common structural features, including several guanine nucleotide binding domains and an effector binding domain. However, exhibiting a remarkable diversity in both structure and function. They are important molecular switches that cycle between the GDP-bound inactive form into the GTP-bound active form through GDP/GTP replacement. In addition, most GTP-binding proteins cycle between membrane-bound and cytosolic forms. such as the RAC family are cytosolic signal transduction proteins that often are involved in processing of extracellular stimuli. Plant RAC proteins are implicated in regulation of plant cell architecture secondary wall formation, meristem signaling, and defense against pathogens. But their molecular mechanisms and functions are not well known. We isolated a RacB homolog from rice to study its role of defense against pathogens. We introduced the constitutively active and the dominant negative forms of the GTP-hinging protein OsRacB into the wild type rice. The dominant negative foms are using two forms (full-sequence and specific RNA interference with RacB). Employing southern, and protein analysis, we examine to different things between the wild type and the transformed plant. And analyzing biolistic bombardment of onion epidermal cell with GFP-RacB fusion protein revealed association with the nucle.

• 한국수자원학회논문집
• /
• 제37권4호
• /
• pp.293-304
• /
• 2004

침전지는 수처리 공정에서 중요한 조작 중 하나이며, 침전지내에서는 응집과 침전이 일어남에 따라 입자의 크기분포가 변하는 복잡한 현상이 발생한다. 따라서 침전지의 효율적인 설계나 운영을 위해서는 이러한 현상에 대해 이해해야만 하며, 침전효율의 극대화를 위한 연구가 필요하다. 본 연구에서는 침전지내의 흐름을 모의하기 위하여 범용 CFD 프로그램인 FLUENT를 이용하였으며, 침전효율을 평가하기 위하여 FLUENT에서 제공되는 입자추적기법을 사용하였다. 또한 침전지의 형상을 지나치게 단순화시키는 기존의 연구와는 달리 본 연구에서는 실제 현장에서 사용되는 규모와 침전지내 인자들 (유입부 정류벽, 유출부 트라프 등)이 수치모의에 최대한 반영되었으며, 현장실험의 결과를 바탕으로 민감도 분석을 수행해 수치모의에 사용되는 매개 변수들을 보정하였다. 민감도 분석 결과 입자의 직경이 입자의 밀도에 비해서 민감도가 큰 것으로 나타났고, 침전효율이 실헐결과와 가장 잘 일치할 때의 직경값을 결정해본 결과 입자의 직경값이 26.5 $\mu\textrm{m}$로 나타났다.

• Molecules and Cells
• /
• 제27권1호
• /
• pp.75-81
• /
• 2009

The Arabidopsis gene AtLEC (At3g15356) gene encodes a putative 30-kDa protein with a legume lectin-like domain. Likely to classic legume lectin family of genes, AtLEC is expressed in rosette leaves, primary inflorescences, and roots, as observed in Northern blot analysis. The accumulation of AtLEC transcript is induced very rapidly, within 30 min, by chitin, a fungal wall-derived oligosaccharide elictor of the plant defense response. Transgenic Arabidopsis carrying an AtLEC promoter-driven ${\beta}$-glucuronidase (GUS) construct exhibited GUS activity in the leaf veins, secondary inflorescences, carpel heads, and silique receptacles, in which no expression could be seen in Northern blot analysis. This observation suggests that AtLEC expression is induced transiently and locally during developmental processes in the absence of an external signal such as chitin. In addition, mechanically wounded sites showed strong GUS activity, indicating that the AtLEC promoter responds to jasmonate. Indeed, methyl jasmonate and ethylene exposure induced AtLEC expression within 3-6 h. Thus, the gene appears to play a role in the jasmonate-/ethylene-responsive, in addition to the chitin-elicited, defense responses. However, chitin-induced AtLEC expression was also observed in jasmonate-insensitive (coi1) and ethylene-insensitive (etr1-1) Arabidopsis mutants. Thus, it appears that chitin promotes AtLEC expression via a jasmonate- and/or ethylene-independent pathway.

• 한국미생물생명공학회:학술대회논문집
• /
• 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
• /
• pp.278-281
• /
• 2004

The $\alpha$1,6-mannosyltransferase encoded by Saccharomyces cerevisiae OCH1 plays a key role for the outer chain initiation of the N-linked oligosaccharides. A search for Hansenula polymorpha genes homologous to S. cerevisiae OCHI (ScOCH1) has revealed seven open reading frames (ORF100, ORF142, ORF168, ORF288, ORF379, ORF576, ORF580). All of the seven ORFs are predicted to be a type II integral membrane protein containing a transmembrane domain near the amino-terminal region and has a DXD motif, which has been found in the active site of many glycosyltransferases. Among this seven-membered OCH1 gene family of H. polymorpha, we have carried out a functional analysis of H. polymorpha ORF168 (HpOCH2) showing the highest identity to ScOCH1. Inactivation of this protein by disruption of corresponding gene resulted in several phenotypes suggestive of cell wall defects, including hypersensitivity to hygromycin B and sodium deoxycholate. The structural analysis of N-glycans synthesized in HpOCH2-disrupted strain (Hpoch2Δ) and the in vitro $\alpha$1,6-mannosyltransferase activity assay strongly indicate that HpOch2p is a key enzyme adding the first $\alpha$1,6-mannose residue on the core glycan Man$_{8}$GlcNAc$_2$. The Hpoch2Δ was further genetically engineered to synthesize a recombinant glycoprotein with the human compatible N-linked oligosaccharide, Man$_{5}$GlcNAc$_2$, by overexpression of the Aspergillus saitoi $\alpha$1,2-mannosidase with the 'HDEL” ER retention signal.gnal.

• Wind and Structures
• /
• 제23권6호
• /
• pp.505-521
• /
• 2016

The objective of this study is to investigate numerically the effect of building roof shaps on wind flow and pollutant dispersion in a street canyon with one row of trees of pore volume, $P_{vol}=96%$. A three-dimensional computational fluid dynamics (CFD) model is used to evaluate air flow and pollutant dispersion within an urban street canyon using Reynolds-averaged Navier-Stokes (RANS) equations and the Explicit Algebraic Reynolds Stress Models (EARSM) based on k-${\varepsilon}$ turbulence model to close the equation system. The numerical model is performed with ANSYS-CFX code. Vehicle emissions were simulated as double line sources along the street. The numerical model was validated by the wind tunnel experiment results. Having established this, the wind flow and pollutant dispersion in urban street canyons (with six roof shapes buildings) are simulated. The numerical simulation results agree reasonably with the wind tunnel data. The results obtained in this work, indicate that the flow in 3D domain is more complicated; this complexity is increased with the presence of trees and variability of the roof shapes. The results also indicated that the largest pollutant concentration level for two walls (leeward and windward wall) is observed with the upwind wedge-shaped roof. But the smallest pollutant concentration level is observed with the dome roof-shaped.

• Mycobiology
• /
• 제42권2호
• /
• pp.167-173
• /
• 2014

A ${\beta}$-glucan synthase gene was isolated from the genomic DNA of polypore mushroom Sparassis crispa, which reportedly produces unusually high amount of soluble ${\beta}$-1,3-glucan (${\beta}$-glucan). Sequencing and subsequent open reading frame analysis of the isolated gene revealed that the gene (5,502 bp) consisted of 10 exons separated by nine introns. The predicted mRNA encoded a ${\beta}$-glucan synthase protein, consisting of 1,576 amino acid residues. Comparison of the predicted protein sequence with multiple fungal ${\beta}$-glucan synthases estimated that the isolated gene contained a complete N-terminus but was lacking approximately 70 amino acid residues in the C-terminus. Fungal ${\beta}$-glucan synthases are integral membrane proteins, containing the two catalytic and two transmembrane domains. The lacking C-terminal part of S. crispa ${\beta}$-glucan synthase was estimated to include catalytically insignificant transmembrane ${\alpha}$-helices and loops. Sequence analysis of 101 fungal ${\beta}$-glucan synthases, obtained from public databases, revealed that the ${\beta}$-glucan synthases with various fungal origins were categorized into corresponding fungal groups in the classification system. Interestingly, mushrooms belonging to the class Agaricomycetes were found to contain two distinct types (Type I and II) of ${\beta}$-glucan synthases with the type-specific sequence signatures in the loop regions. S. crispa ${\beta}$-glucan synthase in this study belonged to Type II family, meaning Type I ${\beta}$-glucan synthase is expected to be discovered in S. crispa. The high productivity of soluble ${\beta}$-glucan was not explained but detailed biochemical studies on the catalytic loop domain in the S. crispa ${\beta}$-glucan synthase will provide better explanations.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2001년도 추계학술대회논문집B
• /
• pp.499-504
• /
• 2001

A validation of a 3D CFD model for predicting local subcooling of moderator in the vicinity of calandria tubes in a CANDU reactor is performed. The small scale moderator experiments performed at Sheridan Park Experimental Laboratory(SPEL) in Ontario, Canada[1] is used for the validation. Also a comparison is made between previous CFD analyses based on 2DMOTH and PHOENICS, and the current model analysis for the same SPEL experiment. For the current model, a set of grid structures for the same geometry as the experimental test section is generated and the momentum, heat and continuity equations are solved by CFX-4.3, a CFD code developed by AEA technology. The matrix of calandria tubes is simplified by the porous media approach. The standard $k-\varepsilon$ turbulence model associated with logarithmic wall treatment and SIMPLEC algorithm on the body fitted grid are used and buoyancy effects are accounted for by the Boussinesq approximation. For the test conditions simulated in this study, the flow pattern identified is a buoyancy-dominated flow, which is generated by the interaction between the dominant buoyancy force by heating and inertial momentum forces by the inlet jets. As a result, the current CFD moderator analysis model predicts the moderator temperature reasonably, and the maximum error against the experimental data is kept at less than $2.0^{\circ}C$ over the whole domain. The simulated velocity field matches with the visualization of SPEL experiments quite well.

• 한국자기학회지
• /
• 제8권1호
• /
• pp.34-41
• /
• 1998

본 연구에서는 비가역 자벽이동 및 결맞음회전 이론을 함께 고려하여 단순 일축 자성체의 돌림힘곡선에서는 둥근(미분가능한) 정점들이 존재하기 위한 조건을 구하고 돌림힘 곡선의 개형을 이론적으로 연구하였다. 일축 비등방성 자성체의 돌림힘 곡선을 인가 자기마당이 변화함에 따라, 이력현상의 유무 및 자기화 역전기구의 종류와 미분 가능한 정점의 갯수에 의해 8가지의 유형들로 구별할 수 있었다. 비등방성 자기마당 세기가 같은 시료라도 자벽의 생성 및 이동에 필요한 최저 임계자기마당 세기가 다르면 상이한 모양의 돌립힘 곡선을 보였다. 한편, 미분 가능한 정점의 높이를 활용하여 포화 자기화량 및 비등방성 에너지 상수를 측정하는 기존의 돌림힘 곡선 해석법들은 인가 자기마당 세기가 비등방성 자기마당 세기의 약 77% 이상일 때 정확한 측정이 가능함을 알 수 있었다.

• JSTS:Journal of Semiconductor Technology and Science
• /
• 제3권3호
• /
• pp.132-138
• /
• 2003

We investigated the polarization retention characteristics of ferroelectric capacitors with $Pb(Zr,Ti)O_3$ (PZT) thin films which were fabricated by different deposition methods. In thermally-accelerated retention tests, PZT films which were prepared by a chemical solution deposition (CSD) method showed rapid decay of retained polarization charges as the thickness of the films decreased down to 100 nm, while the films which were grown by metal organic chemical vapor deposition (MOCVD) retained relatively large non-volatile charges at the corresponding thickness. We concluded that in the CSD-grown films, the thicker interfacial passive layer compared with the MOCVD-grown films had an unfavorable effect on retention behavior. We observed the existence of such interfacial layers by extrapolation of the total capacitance with thickness of the films and the capacitance of these layers was larger in MOCVD-grown films than in CSD-grown films. Due to incomplete compensation of surface polarization charges by the free charges in the metal electrodes, the interfacial field activated the space charges inside the interfacial layers and deposited them at the boundary between the ferroelectric layer and the interfacial layer. Such space charges built up an internal field inside the films, which interfered with domain wall motion, so that retention property at last became degraded. We observed less imprint which was a result of less internal field in MOCVD-grown films while large imprint was observed in CSD-grown films.

• 한국전산유체공학회:학술대회논문집
• /
• 한국전산유체공학회 1996년도 춘계 학술대회논문집
• /
• pp.52-57
• /
• 1996

Mixing is most important for developing an electric washer which transforms angular momentum from rotating solid wall to laundry clothes inside it. For magnification of this mixing effect, some inventions are introduced to washing machine system, i. e., washing plate, washing rod, and even for washing cap in a model of a Korean manufacture. However, the previous efforts show dissatisfaction up till now. In this paper, a triumph to enhance mixing effects to increase washing performance is presented and verified by numerical investigation. The present model to simulate a washing tub is the simple circular cylinder with two endwall disks which is completely filled with a viscous liquid. The present improvement is to change mounting position of a bottom disk of the model cylinder. Therefore, the aim of this work just proposes a new idea, which is numerically inspected, to a producer of washing machine, In detail, this invention is alternating the mounting position of a rotating bottom disk. Actually skewed pulsator is placed in steady of a flat disk, so the two endwall disks at top and bottom are not in parallel. The angle between an inclined bottom disk and the horizontal plane is fixed as 5 degree and physical domain to consider poses a sliced cylinder. Flow fields in both a right circular cylinder and the present improved model are fully depicted by numerical integration on a body fitted nonorthogonal regular uniform grid system. Numerical data to explain flow structure are plotted for understanding of the effects of the inclined disk. Also enhanced mixing effects by the inclined rotating disk are gauged by accurate numerical data used in this work.