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진도개 정액의 연령별 성상 및 동결성에 관한 연구 (Studies on Characteristics and Freezing Tolerance of Spermatozoa in Jindo Dog)

  • 최선호;김성재;조상래;최창용;손준규;김종석;오석일;박병진;김상현
    • Reproductive and Developmental Biology
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    • 제34권3호
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    • pp.253-256
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    • 2010
  • This study was performed to investigate the characteristics within ages and freezing tolerance of spermatozoa in Jindo Dog. Experimental animals were selected 12 herds within 1~8 year's old and collected semen for 2 times in a week. Collected semen was evaluated whole volume and sperm number with CASA system (SIAS, Medical Supply, Korea). Then seminal plasma were separated and diluted with modified Tris-egg yolk extender and added 4, 6 and 8% glycerol for 4 times to final concentration and equilibrated for 1.5 hrs. Before and after freezing, equilibrated semen were evaluated the survival rates. Total volume of sperm at 1~2 year old group is as $5.2{\times}10^8\;cells/ml$ largest and there were no significance among groups. The motility of 1~2 year old group is highest as 90.9% and there were significance among groups. Abnormal sperm showed similar among groups. The survival rate in terms of pre-freezing and post-freezing were decreased all levels of glycerol and reveled 87.0% to 64.5% in 4%, 87.5% to 51.9% in 6% and 73.4% to 29.7% in 8%, there were significant difference among the groups (p<0.05). These results suggest that the optimal sperm-freezing methods in Jindo Dog are utilized with modified Tris egg-yolk extender with 4% glycerol and were improve the reproductive activity by these methods.

Studies on Cd, Pb, Hg and Cr Values in Dog Hairs from Urban Korea

  • Park, S.H.;Lee, M.H.;Kim, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제18권8호
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    • pp.1135-1140
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    • 2005
  • Dogs are a very good indicator of the pollution load on the environment. They share people's environment and are exposed to the action of the same pollutants. This study was to estimate the heavy metal contents in dog hairs in domestic districts, and to assess effects of age, sex, feed habits, living area, breeding environment and smoking habit of owner. The mean concentrations of heavy metals in 204 samples were 0.09${\pm}$0.10 ${\mu}g$/g, 0.21${\pm}$0.09 ${\mu}g$/g, 0.82${\pm}$0.09 ${\mu}g$/g and 0.48${\pm}$0.07 ${\mu}g$/g (Cadmium (Cd), Mercury (Hg), Lead (Pb) and Chromium (Cr)) in dog hairs, respectively. Concentrations of Pb, Cd, Hg and Cr in dog hairs in Yeongnam including Ulsan and Seoul, were higher than in Chungchong and Honam, and concentrations of Pb were significantly different (p<0.01). Concentrations of Cd, Hg, Pb and Cr in dog hairs increased with age (p<0.05). Only dogs fed commercial pet foods had significantly higher Cd and Cr concentrations in hairs than dogs fed dog feed and human diet (p<0.01 in Cd and p<0.05 in Cr). Cr concentrations of dog hairsfrom dogs kept on cement floors were the highest of the other environments (p<0.01). Heavy metal concentrations of dogs owned by smokers, were higher than dogs of non-smokers, but there were no significant differences.

무미와 단미로 특징되는 동경이에 대한 연구 (Study on Dongkyung-i Dog Characterized as Anury and Brachyury)

  • 조성진;박영석;이은수;이현아;김옥진
    • 한국임상수의학회지
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    • 제25권1호
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    • pp.15-18
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    • 2008
  • 최근 댕견으로 불리는 동경이에 대한 과학적 연구는 현재까지 거의 없는 실정이며, 본 연구는 동경이의 형태학적 특징을 조사하기 위하여 수행되었다. 연구 결과 동경이는 활동적이고 체격이 단단하고 사람과 친화적인 것을 알 수 있었다. 형태학적으로 평균 22.6kg의 체중을 보여 중형견으로 분류되었고 피모색은 주로 황갈색으로 짧은 털을 가지고 있었고 외관상 골반이 단단하고 꼬리가 없거나 매우 짧았다. 방사선학적 연구 결과 꼬리의 미추가 형성되지 않았거나 미약한 것을 알 수 있었다. 이러한 연구 결과로부터 한국 고유 품종의 하나인 동경이의 특징은 무미 또는 단미인 것을 알 수 있었다. 향후 혈통에 대한 유전학적 연구의 진행으로 한국 고유의 특성 있는 품종의 개발 가치가 있을 것으로 판단된다.

American cocker spaniel dog에서 발생한 삼첨판 이형성 증례 (Tricuspid valve dysplasia(TVD) in an American cocker spaniel dog)

  • 박철;최치봉;서정향;정병현;박희명
    • 대한수의학회지
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    • 제44권1호
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    • pp.125-129
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    • 2004
  • A 2-year-old, female, American cocker spaniel dog presented for a 1-year history of severe ascites, exercise intolerance, tachypnea. At that time, she was in an emergency state. First, the dog was stabilized with oxygen therapy. A diagnosis of cardiac problem was made from history, auscultation, radiograph, ECG, and echocardiography. Jugular pulsation was palpated and a harsh, systolic murmur of tricuspid regurgitation was prominent at the right cardiac apex. Tricuspid valve dysplasia (TVD) was confirmed with echocardiography, accompanying enormous myocardial hypertrophy. The clinical signs had been improved for 8 months with careful therapy and periodic abdominocentesis, and ascites was well controlled. The situation, however, became worse quickly in a week because the client did not follow our management schedule. Finally, she died due to dyspnea and shock. After the spontaneous death, necropsy and histopathological examination were performed and when we opened the thorax, a significantly large heart was observed. On histopathological findings, grossly myocardium appeared pale initially, then progressed to yellow and white. Microscopically, there was an extensive hemorrhage along with loss of myocardial striations. Interstitial fibrosis and various degenerative alterations in myocytes were also present.

Juvenile onset acquired myasthenia gravis in a Shih-tzu dog

  • Jung, Dong-In;Park, Chul;Kim, Ju-Won;Kim, Ha-Jung;Kang, Byeong-Teck;Lim, Chae-Young;Kang, Myung-Gon;Park, Hee-Myung
    • 대한수의학회지
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    • 제46권1호
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    • pp.71-73
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    • 2006
  • A 7-month-old female Shih-tzu dog was presented with intermittent trembling, dyspnea, generalized muscle weakness, and unconsciousness after exercise. No remarkable findings were shown in the complete blood counts and the radiographic examination. On serum biochemical profiles, alkaline phosphatase and creatine phosphokinase were mildly elevated. Based on history takings, physical examination, and neurological findings, presumptive diagnosis was made as a myasthenia gravis (MG). Clinical signs of this patient were dramatically improved after administration of neostigmine. The result of acetylcholine receptor antibody test in serum was 0.89 nmol/L and the histopathology of muscle were normal. Clinical sign of the patient evaluated in this study is stabilized with long-term administration of pyridostigmine at this time. This case report here describes clinical and clinicopathological findings of a juvenile onset acquired MG in a Shih-tzu dog.

개에서 발생한 전신성 낙엽상 천포창에 사람 면역글로불린의 임상적 적용 (Clinical Trial of Human Intravenous Immunoglobulin in a Dog with Generalized Pemphigus Foliaceus)

  • 박성준
    • 한국임상수의학회지
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    • 제30권1호
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    • pp.61-65
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    • 2013
  • An American Cocker Spaniel (3-year-old, intact female, 6.0 kg) was referred to the Veterinary Medical Teaching Hospital of Chungnam National University for evaluation of pustules and crusts in the periocular region, dorsal and ventral region of the trunk, and digits. Complete blood count (CBC) revealed leukocytosis with mature neutrophilia, and a serum biochemistry profile revealed hypoalbuminemia. Tape strip tests identified numerous neutrophils and acatholytic cells. Histopathology identified intraepithelial pustules with neutrophils and acantholytic keratinocytes. Definitive diagnosis of pemphigus foliaceus (PF) was made by direct immunofluorescence (DIF) test with goat anti-canine IgG antibody. The human intravenous immunoglobulin (IVIG) was administered at a rate of 15 ml/h over 6 hours for 4 days. After that, the dog was maintained on prednisolone (2.2 mg/kg, PO, SID) and azathioprine (2.0 m/kg, PO, SID). An infusion of IVIG (0.5 g/kg) was repeated 3 days after 4 weeks. After 10 weeks, the dog showed the remarkable regression of lesions.

Natural infection of Crenosoma vulpis (Nematoda: Crenosomatidae) in an urban Korean dog

  • Choi, Sungjin;Sim, Cheolho;Kim, Hyeon-Cheol;Choi, Ho-Jung;Park, Bae-Keun
    • 대한수의학회지
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    • 제54권2호
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    • pp.127-129
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    • 2014
  • A male, 3.5 month old Pomeranian dog was diagnosed with a natural infection of Crenosoma (C.) vulpis in Daejeon, Korea. First stage larvae of C. vulpis were detected by fecal examination using the Baermann technique. Thoracic radiographs revealed mild, pervasive bronchial infiltration of the lung. Enumeration of larvae via the McMaster technique revealed 1,600 larvae per gram of feces. The dog was treated with mebendazole, and clinical symptoms were resolved 2 weeks post-treatment, as indicated by the subject presenting fecal tests negative for C. vulpis.

Cricopharyngeal achalasia in an old dog

  • Im, Ji-Eun;Yoon, Hun-Young;Kim, Seung-Gon;Lee, Chang-Min;Eom, Ki-Dong;Park, Hee-Myung
    • 대한수의학회지
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    • 제54권4호
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    • pp.261-263
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    • 2014
  • A 10-year-old castrated male papillon presented with nasal discharge, coughing and chronic dysphagia. On physical examination, the dog exhibited sneezing, gurgling and movement of the throat with repeated attempts to swallow fluids. A diagnosis of cricopharyngeal achalasia (CPA) was made based on video fluoroscopic demonstration of failure of relaxation of the upper esophageal sphincter. This report describes the diagnosis of CPA in an old dog, which is rarely diagnosed in older animals.

Partial Pancreatectomy Using an Ultrasonic-Activated Scalpel in Two Spaniel Dogs with Canine Insulinoma

  • Park, Jiyoung;Lee, Hae-Beom;Jeong, Seong Mok
    • 한국임상수의학회지
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    • 제34권5호
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    • pp.359-365
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    • 2017
  • Two spaniel dogs were presented with a history of lethargy, weakness, refractory hypoglycemia, and episodic seizure. A pancreatic mass on the distal end of the left limb was identified through screening tests including dualphase CT angiography in each patient. Canine insulinoma was suspected with low blood glucose, low fructosamine, and normal to high serum insulin levels. Exploratory laparotomies were performed, and partial pancreatectomy using an ultrasonic-activated scalpel was achieved without enzyme leakage or fistula formation after surgery. The lesions were definitively diagnosed as insulinoma via histopathologic examination. One dog has been doing well until POD 870 with medication; in the other dog, diffuse hepatic nodules noted during surgery were confirmed as a liver metastasis. This dog died at POD 45. This case report addresses the utility of the ultrasonic scalpel in partial pancreatectomy for the treatment of canine insulinoma and differences in prognosis according to disease stage.