• Title/Summary/Keyword: Diversity Component

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Genome-wide Single Nucleotide Polymorphism Analyses Reveal Genetic Diversity and Structure of Wild and Domestic Cattle in Bangladesh

  • Uzzaman, Md. Rasel;Edea, Zewdu;Bhuiyan, Md. Shamsul Alam;Walker, Jeremy;Bhuiyan, A.K.F.H.;Kim, Kwan-Suk
    • Asian-Australasian Journal of Animal Sciences
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    • v.27 no.10
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    • pp.1381-1386
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    • 2014
  • In spite of variation in coat color, size, and production traits among indigenous Bangladeshi cattle populations, genetic differences among most of the populations have not been investigated or exploited. In this study, we used a high-density bovine single nucleotide polymorphism (SNP) 80K Bead Chip derived from Bos indicus breeds to assess genetic diversity and population structure of 2 Bangladeshi zebu cattle populations (red Chittagong, n = 28 and non-descript deshi, n = 28) and a semi-domesticated population (gayal, n = 17). Overall, 95% and 58% of the total SNPs (69,804) showed polymorphisms in the zebu and gayal populations, respectively. Similarly, the average minor allele frequency value was as high 0.29 in zebu and as low as 0.09 in gayal. The mean expected heterozygosity varied from $0.42{\pm}0.14$ in zebu to $0.148{\pm}0.14$ in gayal with significant heterozygosity deficiency of 0.06 ($F_{IS}$) in the latter. Coancestry estimations revealed that the two zebu populations are weakly differentiated, with over 99% of the total genetic variation retained within populations and less than 1% accounted for between populations. Conversely, strong genetic differentiation ($F_{ST}=0.33$) was observed between zebu and gayal populations. Results of population structure and principal component analyses suggest that gayal is distinct from Bos indicus and that the two zebu populations were weakly structured. This study provides basic information about the genetic diversity and structure of Bangladeshi cattle and the semi-domesticated gayal population that can be used for future appraisal of breed utilization and management strategies.

Performance Analysis of DS/CDMA with Diversity and Channel Coding in a Land-Mobile Satellite Channel (육상이동 위성채널에서 다이버시티와 채널 부호를 적용한 DS / CDMA 성능 분석)

  • Kim, Hong-Chil;Kim, Nam
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.8 no.1
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    • pp.42-51
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    • 1997
  • The satellite channel with a line-of-sight signal component is modeled by a shadowed Rician fading channel. We adopt a direct-sequence / code division multiple access (DS / CDMA), which has the advantage to suppress the multipath effect and increase the user capacity. The performance which is evaluated by bit error probability is subjected to the influence of branch number, multi-user number, and spreading code-length. As the result of the analysis, performance advance is achieved with multi-user number decreasing, number of brnaches increasing, and spreading code-length increasing as chip duration is constant. To use both of diversity combining scheme and channel coding is more efficient for performance improvement than the case using diversity combining scheme only. The use of BCH coding and convolutional coding shows better consequence than Hamming coding. Totally, the performance degradation for heavy shadowing is much larger than that for light and average shadowing as heavy shadowing decreases LOS signal.

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Genetic diversity of Indonesian cattle breeds based on microsatellite markers

  • Agung, Paskah Partogi;Saputra, Ferdy;Zein, Moch Syamsul Arifin;Wulandari, Ari Sulistyo;Putra, Widya Pintaka Bayu;Said, Syahruddin;Jakaria, Jakaria
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.4
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    • pp.467-476
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    • 2019
  • Objective: This research was conducted to study the genetic diversity in several Indonesian cattle breeds using microsatellite markers to classify the Indonesian cattle breeds. Methods: A total of 229 DNA samples from of 10 cattle breeds were used in this study. The polymerase chain reaction process was conducted using 12 labeled primers. The size of allele was generated using the multiplex DNA fragment analysis. The POPGEN and CERVUS programs were used to obtain the observed number of alleles, effective number of alleles, observed heterozygosity value, expected heterozygosity value, allele frequency, genetic differentiation, the global heterozygote deficit among breeds, and the heterozygote deficit within the breed, gene flow, Hardy-Weinberg equilibrium, and polymorphism information content values. The MEGA program was used to generate a dendrogram that illustrates the relationship among cattle population. Bayesian clustering assignments were analyzed using STRUCTURE program. The GENETIX program was used to perform the correspondence factorial analysis (CFA). The GENALEX program was used to perform the principal coordinates analysis (PCoA) and analysis of molecular variance. The principal component analysis (PCA) was performed using adegenet package of R program. Results: A total of 862 alleles were detected in this study. The INRA23 allele 205 is a specific allele candidate for the Sumba Ongole cattle, while the allele 219 is a specific allele candidate for Ongole Grade. This study revealed a very close genetic relationship between the Ongole Grade and Sumba Ongole cattle and between the Madura and Pasundan cattle. The results from the CFA, PCoA, and PCA analysis in this study provide scientific evidence regarding the genetic relationship between Banteng and Bali cattle. According to the genetic relationship, the Pesisir cattle were classified as Bos indicus cattle. Conclusion: All identified alleles in this study were able to classify the cattle population into three clusters i.e. Bos taurus cluster (Simmental Purebred, Simmental Crossbred, and Holstein Friesian cattle); Bos indicus cluster (Sumba Ongole, Ongole Grade, Madura, Pasundan, and Pesisir cattle); and Bos javanicus cluster (Banteng and Bali cattle).

Agro-morphological Characterization of Korean, Chinese, and Japanese Adzuki Bean (Vigna angularis (Willd.) Ohwi & Ohashi) Genotypes

  • Kebede Taye Desta;Yu-Mi Choi;Jung-Yoon Yi;Sukyeung Lee;Myoung-Jae Shin;Xiao-Han Wang;Hyemyeong Yoon
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.68 no.1
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    • pp.8-19
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    • 2023
  • Adzuki beans have gained popularity in recent years due to their health benefits. Breeding of Adzuki beans is less favorable than with other legumes due to low genetic diversity. This study aimed to evaluate the genetic diversity of 252 adzuki bean germplasms from China, Japan, and Korea using 18 agro-morphological parameters and comparing their performance to three prominent Korean cultivars: Geomguseul, Arari, and Chungjupat. Leaf shape, pod color, and seed coat color were among the qualitative traits that showed wide variations. The quantitative variables also showed wide variations among adzuki bean germplasms. Although there was no significant difference (p < 0.05), the average rate of germination declined in the order of Korean (91.44%) > Chinese (91.31%) > Japanese (87.47%) adzuki beans. Chinese adzuki beans needed fewer days to flower (DF, 58.22 days) and days to mature (DM, 107.13 days), which varied significantly compared to the Korean and Japanese adzuki beans (p < 0.05). The average number of pods per plant (PPP) and one-hundred seeds weight (HSW) were higher in Japanese adzuki beans compared to the Korean and Chinese adzuki beans although the variation of each was not significant. Almost 29.76% of the accessions had early-blooming flowers, 3.97% were premature, 21.43% produced more PPP, and 3.97% yielded more SPP compared to control cultivars. Results of hierarchical cluster and principal component analyses revealed three clusters with significant variation in all quantitative variables except for RG (p < 0.05). The key factors in multivariate analyses were DF, DM, and HSW. Our study investigated the genetic diversity of adzuki bean accessions and identified ten early maturing and ten high PPP-yielding accessions. Our findings would help farmers and breeders to select the top-performing accessions that can provide them with various options.

Other faunas, coral rubbles, and soft coral covers are important predictors of coral reef fish diversity, abundance, and biomass

  • Imam Bachtiar;Tri Aryono Hadi;Karnan Karnan;Naila Taslimah Bachtiar
    • Fisheries and Aquatic Sciences
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    • v.26 no.4
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    • pp.268-281
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    • 2023
  • Coral reef fisheries are prominent for the archipelagic countries' food sufficiency and security. Studies showed that fish abundance and biomass are affected by biophysical variables. The present study determines which biophysical variables are important predictors of fish diversity, abundance, and biomass. The study used available monitoring data from the Indonesian Research Center for Oceanography, the National Board for Research and Innovation. Data were collected from 245 transects in 19 locations distributed across the Indonesian Archipelago, including the eastern Indian Ocean, Sunda Shelf (Karimata Sea), Wallacea (Flores and Banda Seas), and the western Pacific Ocean. Principal component analysis and multiple regression model were administered to 13 biophysical metrics against 11 variables of coral reef fishes, i.e., diversity, abundance, and biomass of coral reef fishes at three trophic levels. The results showed for the first time that the covers of other fauna, coral rubbles, and soft corals were the three most important predictor variables for nearly all coral reef fish variables. Other fauna cover was the important predictor for all 11 coral reef fish variables. Coral rubble cover was the predictor for ten variables, but carnivore fish abundance. Soft coral cover was a good predictor for corallivore, carnivore, and targeted fishes. Despite important predictors for corallivore and carnivore fish variables, hard coral cover was not the critical predictor for herbivore fish variables. The other important predictor variables with a consistent pattern were dead coral covered with algae and rocks. Dead coral covered with algae was an important predictor for herbivore fishes, while the rock was good for only carnivore fishes.

Classification and Selection of the Breeding materials in the Silkworm, Bombyx mori, by Multivariate Analysis 1. Classification of the Silkworm Genetic Stocks by Principal Component Analysis and Cluster Analysis (다변량 해석법에 의한 누에 육종소재의 탐색 1. 주성분분석과 집락분석을 이용한 누에품종분류)

  • 정도섭;이인정
    • Journal of Sericultural and Entomological Science
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    • v.31 no.2
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    • pp.102-112
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    • 1989
  • Principal component analysis and cluster analysis were performed on the nine quantitative characters of the one hundred and forty eight silkworm genetic stocks. The six major quantitative characters such as cocoon yield, cocoon weight, cocoon shell weight, cocoon shell percentage, larval period of the 5th instar silkworm, and total larval period showed significantly positive correlation between them. The first three principal components extracted form the initial nine variables by principal component analysis accounted for about eighty percent of original information. The first and second principal components were characterized as factors related to silk productivity, and cocoon productivity, respectively. On the basis of multivariate analysis using city block distance determined from the first three principal components to measure the phenotypic diversity, the one hundred and forty eight silkworm genetic stocks could be clustered into seven varietal groups, and the phenotypic diversity between the varietal groups was partly related to their geographical origins. Among 7 varietal group, group II and IV revealed higher silk and cocoon productivity.

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Influence of Growing Green Manures on Soil Microbial Activity and Diversity under Organically Managed Grape-greenhouse (시설 포도 유기농 재배지에서 녹비 생육 중 녹비 종류가 토양 미생물상에 미치는 영향)

  • Park, Kee-Choon;Seo, Young-Jin;Kim, Chan-Yong;Kim, Jong-Su;Yi, Young-Keun;Seo, Ji-Ae
    • Korean Journal of Environmental Agriculture
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    • v.27 no.3
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    • pp.260-266
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    • 2008
  • The aim of present work was to assess the response of soil microbial activity and diversity to green manures under the organically managed grape-greenhouse in early spring. Hairy vetch, milk vetch, and red clover were seeded in fall, and enzymatic activities by dehydrogenase and fluorescein diacetate (FDA) hydrolase, and microbial diversities by Biolog $EcoPlate^{TM}$ and phospholipid fatty acid (PLFA) were characterized for soils sampled in early spring. Dehydrogenase activity and FDA hydrolytic activity did not differentiate the green manures but the average well color development of Biolog EcoPlate was higher in soils covered with red clover than control soil. Soil microbial functional diversity by Biolog EcoPlate differentiated the soils covered with hairy vetch and milk vetch, and Shannon diversity index by Biolog EcoPlate was higher in soils covered with hairy vetch than control soil. Principal component analysis of PLFA differentiated the soils covered with milk vetch from control soil.

SNP-based Genetic Diversity and Relationships Analysis of the Korean Native Black Goat and Crossbred Goat (SNP 정보를 활용한 재래흑염소와 교잡종 염소의 유전적 다양성 및 유연관계 분석)

  • Lee, Sang-Hoon;Lee, Jinwook;Lee, Eun-Do;Kim, Seungchang;Lee, Sung-Soo;Kim, Kwan-Woo
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.21 no.11
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    • pp.102-108
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    • 2020
  • This study was conducted to investigate the genetic diversity and genetic taxonomic relationships between Korean native black goat (KNBG) populations and crossbred goats. The 45,658 common single nucleotide polymorphisms present in the KNBG strain and crossbred goat were used for the analysis. The expected and observed heterozygosity (which can be indicators of genetic diversity) were in the order of crossbred, Gyeongsang National University, Jangsu, then the Tongyeong strains. The variance component represents the degree of genetic diversity between groups. The highest variance (19.98 %) was between the Dangjin and Gyeongsang National University strains. The lowest variance (8.87 %) was between the Jangsu and Tongyeong strains. In addition, the genetic distance between the populations showed that Jangsu and Tongyeong formed one branch (they were very similar genetically). The Dangjin and the Gyeongsang National University strains appeared to form a second branch. Furthermore, the crossbred formed one branch with the Dangjin and the Gyeongsang National University strains. Therefore, the results of this study can be used as basic data to reduce unnecessary inbreeding and genetic resource flow between the KNBG populations. The basic data indicates the uniqueness of the genetic resources of the domestic lineage. These findings provide a basis for differentiating KNBG and Crossbred goats to use to improve the desirable characteristics of this species.

Genetic diversity and population structure in five Inner Mongolia cashmere goat populations using whole-genome genotyping

  • Tao Zhang;Zhiying Wang;Yaming Li;Bohan Zhou;Yifan Liu;Jinquan Li;Ruijun Wang;Qi Lv;Chun Li;Yanjun Zhang;Rui Su
    • Animal Bioscience
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    • v.37 no.7
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    • pp.1168-1176
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    • 2024
  • Objective: As a charismatic species, cashmere goats have rich genetic resources. In the Inner Mongolia Autonomous Region, there are three cashmere goat varieties named and approved by the state. These goats are renowned for their high cashmere production and superior cashmere quality. Therefore, it is vitally important to protect their genetic resources as they will serve as breeding material for developing new varieties in the future. Methods: Three breeds including Inner Mongolia cashmere goats (IMCG), Hanshan White cashmere goats (HS), and Ujimqin white cashmere goats (WZMQ) were studied. IMCG were of three types: Aerbas (AEBS), Erlangshan (ELS), and Alashan (ALS). Nine DNA samples were collected for each population, and they were genomically re-sequenced to obtain high-depth data. The genetic diversity parameters of each population were estimated to determine selection intensity. Principal component analysis, phylogenetic tree construction and genetic differentiation parameter estimation were performed to determine genetic relationships among populations. Results: Samples from the 45 individuals from the five goat populations were sequenced, and 30,601,671 raw single nucleotide polymorphisms (SNPs) obtained. Then, variant calling was conducted using the reference genome, and 17,214,526 SNPs were retained after quality control. Individual sequencing depth of individuals ranged from 21.13× to 46.18×, with an average of 28.5×. In the AEBS, locus polymorphism (79.28) and expected heterozygosity (0.2554) proportions were the lowest, and the homologous consistency ratio (0.1021) and average inbreeding coefficient (0.1348) were the highest, indicating that this population had strong selection intensity. Conversely, ALS and WZMQ selection intensity was relatively low. Genetic distance between HS and the other four populations was relatively high, and genetic exchange existed among the other four populations. Conclusion: The Inner Mongolia cashmere goat (AEBS type) population has a relatively high selection intensity and a low genetic diversity. The IMCG (ALS type) and WZMQ populations had relatively low selection intensity and high genetic diversity. The genetic distance between HS and the other four populations was relatively high, with a moderate degree of differentiation. Overall, these genetic variations provide a solid foundation for resource identification of Inner Mongolia Autonomous Region cashmere goats in the future.

Bird Diversity on Area around the Ulsan Mosaic Landscape (울산지역 모자익 경관에서의 조류 다양성)

  • Lee, Won-Ho;Jang, Ji-Doek;Choi, Byung-In;Kang, Sung-Ryong;Kwon, Ki-Chung
    • The Korean Journal of Ecology
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    • v.27 no.6 s.122
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    • pp.325-333
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    • 2004
  • Birds were censused to investigate the composition of landscape structure for bird diversity around Ulsan between May and November 2002. Associations with three main categories of habitat variables were evaluated: 1) amount and type of forest; 2) residual habitats not classified as forest or crops; 3) land-use variables. Cluster analysis of bird community shows the highest forest variables of $79.06\%$, and the others are residual habitat variables ($17.98\%$), land-use variables ($2.94\%$) in spring, and forest variables of $57.77\%$, land-use variables ($23.16\%$), residual habitat variables ($18.47\%$) in autumn, respectively. In Principal Component Analysis of a total of 196 sites, the populations are strongly correlated to Component I ($54.8\%$) based forest habitats and to Component II based on land-use. Species preferring sites were clearly separated with heterogenous forest along the first axis. In autumn, the populations are moderately correlated to Component I based land-use and to component II based forest habitats. Species preferring local habitats were also clearly separated. Fifty three species of 1,700 birds were recorded: Brown-eared Bulbul, Vinous-throated Parrotbill, Great Tit, Tree Sparrow and Black-billed Magpie accounted for over $60\%$ of the observed birds in spring and autumn. The important species were Brown-eared Bulbul, Vinous-throated Parrotbill, Great Tit and Tree Sparrow in spring and autumn. Four habitats in terms of their species richness were computed as follows: Wonhyosan has the highest an expected species number, $E[S_{59}]=19$. Moonsusan has the lowest expected species number, $E[S_{59}]=17$ in spring. In autumn, Kuenamsan has the highest expected species number, $E[S_{63}]=16$. Moonsusan has the lowest expected species number, $E[S_{63}]=12$. Pairwise similarity declined with increasing distance between recording site and recording site from Moonsusan-Wonhyosan (0.62), the same geographical regions clustered separately in a UPGMA cluster tree in spring, and in autumn from Moonsusan-ChungJoksan (0.53).