• Korean Journal of Soil Science and Fertilizer
• /
• v.19 no.4
• /
• pp.339-344
• /
• 1986

Frost damage which can reduce yields, impair fruit quality and cause loss of trees is closely related to the occurrence of daily minimum temperature. Horizontal distribution of air temperature minima can be characterized by conditions of radiational cooling and gravitational movement of cold air, which are influenced by the regional topographic features. Observations were made on the air temperature minima over Yangpyeong area, to delineate potential effects of topography on the temperature pattern during spring season. Two routes were selected for the observation. Liquid glass minimum thermometers were installed at 17 sites through the old peach orchards which had been closed due to the frequent freeze-frost hazards during the recent years. This route was 8.5km long and the highest point was 350m above mean sea level. The other route, which was 2.5km in distance, was run with a digital resistance thermometer during the hour just before sunrise. Observations were made both on a calm-clear day (April 30, 1986) and a windy-overcast day (May 1, 1986). The temperature on April 30 was in increasing trend with elevation but this was modified at near the riverside and the downtown area. An orchard lying on a hilltop showed the temperature $1^{\circ}C$ higher than near by lowland of which elevation was about 30m lower. The minimum temperature on the overcast day was little affected by terrestrial conditions but by the atmospheric lapse condition. The peach orchards severely damaged by cold air were found in the area where the lowest minimum temperature was observed. The results may be useful for selection of the proper orchard location to be developed in an area.

• Journal of Korean Society of Forest Science
• /
• v.86 no.2
• /
• pp.241-250
• /
• 1997

The study was conducted to make a map for selecting righteous tree species for each site by digital terrain analysis. We set an algorithmic value for each tree species' characteristics with distribution pattern analysis, and the soil types were digitized from data indicated on soil map. Mean altitude, slope, aspect and micro-topography were estimated from the digital map for each block which had been calculated by regression equations with altitude. The results obtained from the study could be summarized as follows 1. We could develope a method to select righteous tree species for a given site with concern of soil, forest condition and topographic factors on Muju-Gun in Chonbuk province(2,500ha) by the terrain analysis and multi-variate digital map with a personal computer. 2. The brown forest soils were major soil types for the study area, and 29 tree species were occurred with Pinus densiflora as a dominant species. The differences in site condition and soil properties resulted in site quality differences for each tree species. 3. We tried to figure out the accuracy of a basic program(DTM.BAS) enterprised for this study with comparing the mean altitude and aspect calculated from the topographic terrain analysis map and those from surveyed data. The differences between the values were less than 5% which could be accepted as a statistically allowable value for altitude, as well as the values for aspect showed no differences between both the mean altitude and aspect. The result may indicate that the program can be used further in efficiency. 4. From the righteous-site selection map, the 2nd group(R, $B_1$) took the largest area with 46% followed by non-forest area (L) with 23%, the 5th group with 7% and the 4th group with 5%, respectively. The other groups occupied less than 6%. 5. We suggested four types of management tools by silvicultural tree species with considering soil type and topographic conditions.

• Journal of Korean Society of Forest Science
• /
• v.48 no.1
• /
• pp.51-58
• /
• 1980

Translocation of $^{14}C$-labelled photosynthate from needles to terminal buds of Pinus elliottii was studied to understand a pattern of mobilization of photosynthate to buds during the period of female strobilus initiation. Current-year shoots of 20-year-old trees were exposed in 1ate July and late August to $50{\mu}$ Ci of $^{14}CO_2$ for 90 minutes, and harvested 1 day and 7 days after the exposure to analyze distribution of $^{14}C$ in ethanol-soluble and ethanol-insoluble fractions. Over 85% of total radioactivity of bud tissue one day after the exposure and 55% total activity seven days after the exposure remained in the ethanol-soluble fraction. The amount of $^{14}C$ translocation into female-producing buds in late August was significantly less than into vegetative and male-producing buds both one day and seven days after the exposure. When the ethanol­soluble fraction was further fractionated into hexane-soluble, amino acid, sugar, and organic acid fractions, radioactivity in the female-producing buds in late August was about a third of that in male-producing buds in all the fractions. This indicated that the male-producing buds required in late August but not in late July mobilization of large amount of photosynthate for developing male strobilus primordia. Fema1e-producing buds, on the other hand, did not mobilize as much photosynthate as male-producing buds in late August when female strobilus primordia are believed to be initiated. It is suggested that initiation of the female strobilus primordia is associated with temporary reduction in the metabolic activity of the terminal buds in slash pine.

• Korean journal of applied entomology
• /
• v.47 no.4
• /
• pp.337-343
• /
• 2008

This study was conducted to investigate the distribution pattern, ecological characteristics and life cycle of the Black Soldier Fly (Hermetia illucens, BSF). The BSF was widely distributed throughout Korea. The insect was mainly found in the vicinity of and in cattle sheds, manure sheds, living waste dump grounds, and food waste dump grounds. Developmental characteristics of the BSF are as follows: the egg was long oval shaped of 887 ${\mu}m$ in the major axis and 190 ${\mu}m$ in the minor axis; it weighed 24 ${\mu}g$. Female oviposited ca. 1,000 eggs on average; eggs hatched in 81 hours under laboratory condition ($27^{\circ}C$, 60% R.H.). The duration of the larval stage was approximately $15{\sim}20$ days. The size of the last instar larvae was 21 mm. The cuticle of the pupae gradually acquired red-brown color and the size of them was 19 mm. The pupal stage was shorter for females (16 days) than males (15 days). Adults were sized about $13{\sim}20$ mm long and black-colored. The life span of adult insects was $5{\sim}8$ days for the first generation (June${\sim}$July), $7{\sim}10$ days for the second generation (Aug.${\sim}$Sept.), and $13{\sim}18$ days for the third generation (Sept.${\sim}$Oct.). Mating started on the next day of emergence and actively occurred at the third day after emergence. Mating mostly occurred between 10:00 and 16:00 during which light intensity is highest. Egg-laying started on the third day and was most frequent from the fourth to the sixth day after emergence. Similar to mating time, females oviposited mostly between 10:00 and 16:00.

• Journal of the Mineralogical Society of Korea
• /
• v.28 no.1
• /
• pp.71-81
• /
• 2015

This study was carried out in order to investigate the mineralogical characteristics of the core sediments (03GHP-02 and HB13-2), obtained from the Ulleung Basin and Hupo Basin, Korea. The results on mineral compositions, clay mineral compositions, and the total contents and sequential extraction of different fractions of the phosphorus in core samples showed that those values are different in two cores and also at different depths. In both samples, mineral compositions were the same, composed mainly of quartz, microcline, albite, calcite, opal A, pyrite, and clay minerals (illite, chlorite, kaolinite, and smectite). However, the sample from Hupo Basin contains more opal A. Both samples, especially the ones from Hupo Basin contains more smectite than those reported from East Sea, indicating the influence of paleo-Hwangwei River and the Tertiary Formation of Korea Peninsula. For the samples from Uleung Basin, at 0.7-3.5 m range in depth, the low content of opal A and the low illite crystallinity index can be inferred to indicate the relatively cool climate, corresponding to the ice age. Also, the content of total phosphorus was low in those samples. It was reported that East Sea at that time was isolated from the neighboring seas due to the decrease of the sea level, and as a result, the influx of sediments was supposed to be little through the strait and rivers. For the samples from Hupo Basin, there is no significant changes in clay mineral composition and the distribution of phosphorus with increasing depth. This little change can be interpreted to indicate that the sediments comprising the core might be deposited in a relatively short period of time or deposited in sedimentary environment in which there's no significant changes in sediment supplies. The values of crystallinity index of clay minerals are high in those samples, indicating that it was relatively warm during that time. Although the increase of fluctuation pattern can be observed, showing that the climate of this period often changed, it is supposed that it was generally warm.

• 한국균학회소식:학술대회논문집
• /
• 2014.05a
• /
• pp.27-28
• /
• 2014

Beauveria bassiana (Cordycipitaceae, Hypocreales, Ascomycota) is an anamorphic fungus having a potential to be used as a biological control agent because it parasitizes a wide range of arthropod hosts including termites, aphids, beetles and many other insects. A number of bioactive secondary metabolites (SMs) have been isolated from B. bassiana and functionally verified. Among them, beauvericin and bassianolide are cyclic depsipeptides with antibiotic and insecticidal effects belonging to the enniatin family. Non-ribosomal peptide synthetases (NRPSs) play a crucial role in the synthesis of these secondary metabolites. NRPSs are modularly organized multienzyme complexes in which each module is responsible for the elongation of proteinogenic and non-protein amino acids, as well as carboxyl and hydroxyacids. A minimum of three domains are necessary for one NRPS elongation module: an adenylation (A) domain for substrate recognition and activation; a tholation (T) domain that tethers the growing peptide chain and the incoming aminoacyl unit; and a condensation (C) domain to catalyze peptide bond formation. Some of the optional domains include epimerization (E), heterocyclization (Cy) and oxidation (Ox) domains, which may modify the enzyme-bound precursors or intermediates. In the present study, we analyzed genomes of B. bassiana and its allied species in Hypocreales to verify the distribution of NRPS-encoding genes involving biosynthesis of beauvericin and bassianolide, and to unveil the evolutionary processes of the gene clusters. Initially, we retrieved completely or partially assembled genomic sequences of fungal species belonging to Hypocreales from public databases. SM biosynthesizing genes were predicted from the selected genomes using antiSMASH program. Adenylation (A) domains were extracted from the predicted NRPS, NRPS-like and NRPS-PKS hybrid genes, and used them to construct a phylogenetic tree. Based on the preliminary results of SM biosynthetic gene prediction in B. bassiana, we analyzed the conserved gene orders of beauvericin and bassianolide biosynthetic gene clusters among the hypocrealean fungi. Reciprocal best blast hit (RBH) approach was performed to identify the regions orthologous to the biosynthetic gene cluster in the selected fungal genomes. A clear recombination pattern was recognized in the inferred A-domain tree in which A-domains in the 1st and 2nd modules of beauvericin and bassianolide synthetases were grouped in CYCLO and EAS clades, respectively, suggesting that two modules of each synthetase have evolved independently. In addition, inferred topologies were congruent with the species phylogeny of Cordycipitaceae, indicating that the gene fusion event have occurred before the species divergence. Beauvericin and bassianolide synthetases turned out to possess identical domain organization as C-A-T-C-A-NM-T-T-C. We also predicted precursors of beauvericin and bassianolide synthetases based on the extracted signature residues in A-domain core motifs. The result showed that the A-domains in the 1st module of both synthetases select D-2-hydroxyisovalerate (D-Hiv), while A-domains in the 2nd modules specifically activate L-phenylalanine (Phe) in beauvericin synthetase and leucine (Leu) in bassianolide synthetase. antiSMASH ver. 2.0 predicted 15 genes in the beauvericin biosynthetic gene cluster of the B. bassiana genome dispersed across a total length of approximately 50kb. The beauvericin biosynthetic gene cluster contains beauvericin synthetase as well as kivr gene encoding NADPH-dependent ketoisovalerate reductase which is necessary to convert 2-ketoisovalarate to D-Hiv and a gene encoding a putative Gal4-like transcriptional regulator. Our syntenic comparison showed that species in Cordycipitaceae have almost conserved beauvericin biosynthetic gene cluster although the gene order and direction were sometimes variable. It is intriguing that there is no region orthologous to beauvericin synthetase gene in Cordyceps militaris genome. It is likely that beauvericin synthetase was present in common ancestor of Cordycipitaceae but selective gene loss has occurred in several species including C. militaris. Putative bassianolide biosynthetic gene cluster consisted of 16 genes including bassianolide synthetase, cytochrome P450 monooxygenase, and putative Gal4-like transcriptional regulator genes. Our synteny analysis found that only B. bassiana possessed a bassianolide synthetase gene among the studied fungi. This result is consistent with the groupings in A-domain tree in which bassianolide synthetase gene found in B. bassiana was not grouped with NRPS genes predicted in other species. We hypothesized that bassianolide biosynthesizing cluster genes in B. bassiana are possibly acquired by horizontal gene transfer (HGT) from distantly related fungi. The present study showed that B. bassiana is the only species capable of producing both beauvericin and bassianolide. This property led to B. bassiana infect multiple hosts and to be a potential biological control agent against agricultural pests.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.21 no.1
• /
• pp.24-35
• /
• 2016

To assess the relationship between environmental factors and seasonal phytoplankton community structure, we investigated abiotic and biotic factors in Ulsan Bay, Korea. We divided the bay into two areas based on geographical characteristics and compared the difference in each factor between inner and outer bay with t-test statistics. As a result, temperature in the outer bay was higher than that of the inner bay during winter (t = -5.833, p < 0.01) and autumn (p > 0.05). However, opposite trend was observed during spring (t = 4.247, p < 0.01) and summer (t = 2.876, p < 0.05). Salinity was significantly lower in the inner bay than in the outer bay in winter, spring, and summer (p < 0.01). However, the salinity was not significantly different between the inner and the outer bay in the autumn (p > 0.05). In particular, high nutrient concentration was observed in most stations during winter season due to vertical well mixing. The nutrient concentration was significantly higher in surface layers of inner bay after rainfall, particularly in the summer. The relative contribution (approximately 70%) of < $20{\mu}m$ (nano and pico) size phytoplankton was increased in all seasons with continuously low nutrients from the offshore water due to their adaption to low nutrient without other large competitors. Interestingly, high population of Eutreptiella gymnastica was kept in the inner bay during the spring and summer associated with high DIN (nitrate+nitrite, ammonium) after river discharge following rainfall, suggesting that DIN supply might have triggered the increase of Eutreptiella gymnastica population. In addition, high density of freshwater species Oscillatoria sp. and Microcystis sp. were found in several stations of the inner bay that were provided with large amounts of freshwater from the Tae-wha River. Diatom and cryptophyta species were found to be dominant species in the autumn and winter. Of these, centric diatom Chaetoceros genus was occupied in the outer bay in the autumn. Cryptophyta species known as opportunistic micro-algae were found to have high biomass without competitors in the inner bay. Our results demonstrated that Ulsan Bay was strongly affected by freshwater from Tae-wha River during the rainy season and by the surface warm water current from the offshore of the bay during dry season. These two external factors might play important roles in regulating the seasonal phytoplankton community structures.

• The korean journal of orthodontics
• /
• v.35 no.3 s.110
• /
• pp.207-215
• /
• 2005

This study was performed to evaluate clinical practicality of the rebonding method with flowable resin without the removal of the residual resin on the debonded theeth and debonded bracket base after debonding. The samples of the control group (group I) were rebonded with Transbond XT using the usual rebonding method after the residual resin was removed. At experimental group, the brackets were rebonded with Transbond XT(group II) and CharmFil Flow (group III) without removal of residual resin which is the possibility becoming the index (or rebonding to similar position With initial bonding. The Shear bond Strength of the each group was measured. Patterns of bonding failure were evaluated with modified ARI score. and the shear bond strength according to patterns of bonding failure at experimental group was compared. Between the control group $(6.51\pm1.21MPa)$ and the group II rebonded with Transbond XT $(6.30\pm1.01MPa)$ did not have significantly difference in the shear bond strength (p=0.534), and the shear bond strength of group II was Significantly lower 4han the group III rebonded With CharmFil Flow $(7.29\pm1.54 MPa)$ (P=0.009). At control group, there was not large difference if distribution of bending failure pattern. But at experimental group, bond failure did not occur in interface between the resin-enamel. and bond failure between the resin-bracket, within the resin was distributed similarly. There was not significantly difference in the shear bond strength according to patterns of bonding failure at experimental group (P>0.05) The result of this study showed that the method suggested in this study aid flowable resin as rebonding adhesive could be useful in clinically.

• Economic and Environmental Geology
• /
• v.38 no.6 s.175
• /
• pp.607-622
• /
• 2005

This study was carried out in order to evaluate where the soil loss was mainly occurred, .and to verify how riverbed sediments in the tributaries of the Seomjin River were related to their source rocks distributed in Sunchang area. The study area including the Seomjin River with 4 tributaries of Kyeongcheon, Okgwacheon, Changjeong-cheon and Ipcheon was divided into 10 watershed. The RUSLE (Revised Universal Soil Loss Equation) was estimated for all the grids (10 m cells) in the corresponding watershed. The amount of soil loss per unit area was calculated as follows: dry fold (53,140.94 tons/ha/year), orchard (25,063.38 tons/ha/year), paddy field (6,506.7 tons/ha/year) and Idlest (6,074.36 tons/ha/year). The differences of soil loss per unit area appear to be depends on areas described earlier. Soil erosion hazard zones were generally distributed within dry fields. Several thematic maps such as land use maps, topographical maps and soil maps were used as a data to generate the RUSLE factors. The amount of soil loss, computed by using the RUSLE, showed that soil loss mainly occurred at the regions where possible source rocks were distributed along the stream. Based on the this study on soil loss and soil erosion hazard zone together with chondrite-normalized REE patterns that were previously analyzed in same study area, a closed relationship between riverbed sediments and possible source rocks is formed. Especially in the Okgwacheon that are widely distributed by various rocks, chondrite-normalized REE pattern derived from the riverbed sediments, source rock and soil is expected to have a closed relationship with the distribution of soil loss.

• Journal of Biomedical Engineering Research
• /
• v.24 no.2
• /
• pp.133-140
• /
• 2003

In this study, muscle activity was measured using surface EMG (sEMG) during a voluntary maneuver (ankle dorsiflexion) in the supine position was compared pre and post gait training. Nine patients with incomplete spinal cord injury participated in a supported treadmill ambulation training (STAT), twenty minutes a day, five days a week for three months. Two tests, a gait speed test and a voluntary maneuver test, were made the same day, or at least the same week, pre and post gait training. Ten healthy subjects' data recorded using the same voluntary maneuvers were used for the reference. sEMG measured from ten lower limb muscles was used to observe the two features of amplitude and motor control distribution pattern, named response vector. The result showed that the average gait speed of patients increased significantly (p〈0.1) from 0.47$\pm$0.35 m/s to 0.68$\pm$0.52 m/s. In sEMG analysis, six out of nine patients showed a tendency to increase the right tibialis anterior activity during right ankle dorsiflexion from 109.7$\pm$148.5 $mutextrm{V}$ to 145.9$\pm$180.7 $mutextrm{V}$ but it was not significant (p〈0.055). In addition, only two patients showed increase of correlation coefficient and total muscle activity in the left fide during left dorsiflexion. Patients' muscle activity changes after gait training varied individually and generally depended on their muscle control abilities of the pre-STAT status. Response vector being introduced for quantitative analysis showed good Possibility to anticipate. evaluate, and/or guide patients with SCI, before and after gait training.