• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.64-64
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• 2019

In order to evaluate the taxonomical relevance of seeds morphological characteristics, a macroand micro-morphological study was conducted on seeds of Korean Vicia (Fabacea). We collected 19 taxa of genus Vicia distributed in Korea and introduced one taxa from USDA. The morphological characteristic and testa texture of seeds were investigated using a Stereo-microscope (SM) and Scanning Electron Microscope (SEM). Most of Vicia seeds were found spherical or oblong and some seeds were oval and subglose. The largest seed was V. chosenensis ($4.3{\times}3.6{\times}2.6mm$), and the smallest was V. teterasperma ($1.7{\times}1.7{\times}1.5mm$). V. chosenensis and V. hirsuta were separated from other Vicia species by having a shiny in seed finish. In hilum shape, 14 species have linear and V. sepium was distinguished by having a circumlinear. In testa texture, they developed papilae, only V. hirsuta has lophate in level type. Deposition of the sheet-like debris between the papilae was observed in V. chosenensis, V. cracca, and V. unijuga. Polymorphism information content (PIC) values of the 13 qualitative morphological characters (QMC) were in the range of 0.0950 to 8863 with an average of 0.4611. PIC value of seed shape, seed colour, hilum colour were 0.7403, 0.8177, 0.883 respectively. Cluster analysis based on QMC detected three main clades. V. cracca, V. amurensis, V. amoena were involved in Group 1 and V. unijuga f. minor, V. unijuga, V. unijuga f. angustifolia, V. sepium, V. hirticalycina, V. hirsuta, V. linearfolia, V. chosenensis, V. pseudorobus, V. venosa var. cuspidata were involved in Group 2. V. nipponica, V. japonica, V. villosa, V. dasicarpa, V. bungei, V. angustifolia, V. tetrasperma were clustered in Group 3. Our research suggests that morphological characteristic and testa texture of seeds could be used as definers for the identification of genus Vicia.

• Proceedings of the Korea Water Resources Association Conference
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• 2018.05a
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• pp.202-202
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• 2018

본 논문에서는 분포형 강우-유출 모형인 GRM(Grid based Rainfall-runoff Model)(최윤석, 김경탁, 2017)을 이용해서 낙동강 유역을 대상으로 대유역 홍수해석시스템을 구축하고, 유출해석을 위한 실행시간을 평가하였다. 유출모형은 낙동강의 주요 지류와 본류를 소유역으로 구분하여 모형을 구축하고, 각 소유역의 유출해석 결과를 실시간으로 연계할 수 있도록 하여 낙동강 전체 유역의 유출모형을 구축하였다. 이와 같이 하나의 대유역을 다수의 소유역시스템으로 분할하여 모형을 구축할 경우, 유출해석시스템 구성이 복잡해지는 단점이 있으나, 소유역별로 각기 다른 자료를 이용하여 다양한 해상도로 유출해석을 할 수 있으므로, 소유역별 특성에 맞는 유출모형 구축이 가능한 장점이 있다. 또한 각 소유역시스템은 별도의 프로세스로 계산이 진행되므로, 대유역을 고해상도로 해석하는 경우에도 계산시간을 단축할 수 있다. 본 연구에서는 낙동강 유역을 20개(본류 구간 3개, 1차 지류 13개, 댐상류 4개)의 소유역으로 분할하여 계산 시간을 검토하였으며, 최종적으로 21개(본류 구간 3개, 1차 지류 13개, 댐상류 5개)의 소유역으로 분할하여 유출해석시스템을 구축하였다. 댐 상류 유역은 댐하류와 유량전달이 없이 독립적으로 모의되고, 댐과 연결된 하류 유역은 관측 방류량을 상류단 하천의 경계조건으로 적용한다. 지류 유역은 본류 구간과 연결되고, 지류의 계산 유량은 본류와의 연결지점에 유량조건으로 실시간으로 입력된다. 이때 본류와 지류의 유량 연계는 데이터베이스를 매개로 하였다. 유출해석시스템의 성능을 평가하기 위해서 Microsoft 클라우드 서비스인 Azure를 이용하였다. 낙동강 유역을 20개 소유역으로 구성한 경우에서의 유출해석시스템의 속도 평가 결과 Azure virtual machine instance DS15 v2(OS : Windows Server 2012 R2, CPU : 2.4 GHz Intel $Xeon^{(R)}$ E5-2673 v3 20 cores)에서 1.5분이 소요 되었다. 계산시간 평가시 GRM은 'IsParallel=false' 옵션을 적용하였으며, 모의 기간은 24시간을 기준으로 하였다. 연구결과 분포형 모형을 이용한 대유역 유출해석시스템 구축이 가능했으며, 계산시간도 충분히 단축할 수 있었다. 또한 추가적인 CPU와 병렬계산을 적용할 경우, 계산시간은 더 단축될 수 있으며, 이러한 기법들은 분포형 모형을 이용한 대유역 유출해석시스템 구축시 유용하게 활용될 수 있을 것으로 판단된다.

• Proceedings of the KSAR Conference
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• 2001.03a
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• pp.68-68
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• 2001

Blastocyst formation, consisting of the inner cell mass (ICM) and trophectoderm (TE), is the first differentiation process during embryonic development in mammals. It has been hypothesized that the proportion of ICM to TE in the blastocyst may be crucial for subsequent developmental competence of early embryos, which it may be expressed as a sensitive indicator for evaluating in vitro systems. In this study ICM/total cell ratio of nuclear transfer (NT) embryos was compared with IVF-derived and in vivo embryos. Somatic cell nuclei obtained from a fetus at Day 40 of gestation were transferred into the enucleated oocyte and then cultured in NCSU 23 medium for 6 days as previously described (Koo et al., Biol. Reprod. 2000; 63:986-992). ICM and TE cells of blastocysts were determined by using a differential staining method (Han et al., Biol. Reprod. 1999; 60:1110-1113). Development rate (9.8$\pm$2.5%, 23/225) to the blastocyst stage of NT embryos was lower than IVF embryos (23.8$\pm$2.7%, 53/223). Thus, a difference was detected in the in vitro developmental rate to blastocyst stage between NT and IVF-derived embryos (P<0.05). In the next experiment, we investigated ICM and TE nuclei to assess the quality of blastocysts that produced by NT, IVF and in vivo, respectively. NT blastocysts (27.6$\pm$8.3) showed a smaller total cell number than IVF-derived (42.6$\pm$17.4) and in vivo embryos (283.9$\pm$103.5) (P<0.05). Ratios of ICM/total cells in NT, IVF and in vivo blastocysts were 15.1$\pm$ 18.6% (n=56), 12.3$\pm$9.2% (n=57) and 30.4$\pm$6.8% (n=40), respectively. Individual blastocysts for the ratio of ICM/total cells were assigned to 3 groups (I; <20%, II; 20 to 40% and III;>40%). As the results, most in vivo blastocysts (97.5%, 39/40) were distributed into group II while most NT (78.6%, 44/56) and IVF-derived blastocysts (82.5%, 47/57) were allocated to group I. Thus, our data show that NT or IVF-derived embryos have aberrant morphology during early development in vitro systems, suggesting that these anomalies may result in developmental failures of the NT embryos to term.

• International conference on construction engineering and project management
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• 2022.06a
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• pp.1243-1244
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• 2022

In recent years, the growing interest in off-site construction has led to factories scaling up their manufacturing and production processes in the construction sector. Consequently, continuous large-scale site monitoring in low-variability environments, such as prefabricated components production plants (precast concrete production), has gained increasing importance. Although many studies on computer vision-based site monitoring have been conducted, challenges for deploying this technology for large-scale field applications still remain. One of the issues is collecting and transmitting vast amounts of video data. Continuous site monitoring systems are based on real-time video data collection and analysis, which requires excessive computational resources and network traffic. In addition, it is difficult to integrate various object information with different sizes and scales into a single scene. Various sizes and types of objects (e.g., workers, heavy equipment, and materials) exist in a plant production environment, and these objects should be detected simultaneously for effective site monitoring. However, with the existing object detection algorithms, it is difficult to simultaneously detect objects with significant differences in size because collecting and training massive amounts of object image data with various scales is necessary. This study thus developed a large-scale site monitoring system using edge computing and a small-object detection system to solve these problems. Edge computing is a distributed information technology architecture wherein the image or video data is processed near the originating source, not on a centralized server or cloud. By inferring information from the AI computing module equipped with CCTVs and communicating only the processed information with the server, it is possible to reduce excessive network traffic. Small-object detection is an innovative method to detect different-sized objects by cropping the raw image and setting the appropriate number of rows and columns for image splitting based on the target object size. This enables the detection of small objects from cropped and magnified images. The detected small objects can then be expressed in the original image. In the inference process, this study used the YOLO-v5 algorithm, known for its fast processing speed and widely used for real-time object detection. This method could effectively detect large and even small objects that were difficult to detect with the existing object detection algorithms. When the large-scale site monitoring system was tested, it performed well in detecting small objects, such as workers in a large-scale view of construction sites, which were inaccurately detected by the existing algorithms. Our next goal is to incorporate various safety monitoring and risk analysis algorithms into this system, such as collision risk estimation, based on the time-to-collision concept, enabling the optimization of safety routes by accumulating workers' paths and inferring the risky areas based on workers' trajectory patterns. Through such developments, this continuous large-scale site monitoring system can guide a construction plant's safety management system more effectively.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.78-78
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• 2002

The role of heat shock proteins in shielding organism from environmental stress is illustrated by the large-scale synthesis of these protein by the organism studied to date. However, recent evidence also suggests an important role for heat shock protein in fertilization and early development of mammalian embryos. Effects of elevated in vitro temperature on in vitro produced bovine embryos were analysed in order to determine its impact on the expression of heat shock protein 70 (HSP70) by control and frozen-thawed after in vitro fertilization (IVF) or nuclear transfer (NT). The objective of this study was to assess the developmental potential in vitro produced embryos with using of the various containers and examined expression and localization of heat shock protein 70 after it's frozen -thawed. For the vitrification, in vitro produced embryos at 2 cell, 8 cell and blastocysts stage after IVF and NT were exposed the ethylene glycol 5.5 M freezing solution (EG 5.5) for 30 sec, loaded on each containers such EM grid, straw and cryo-loop and then immediately plunged into liquid nitrogen. Thawed embryos were serially diluted in sucrose solution, each for 1 min, and cultured in CRI-aa medium. Survival rates of the vitrification production were assessed by re-expanded, hatched blastocysts. There were no differences in the survival rates of IVF using EM grid, cryo-loop. However, survival rates by straw were relatively lower than other containers. Only, nuclear transferred embryos survived by using cryo-loop. After IVF or NT, in vitro matured bovine embryos 2 cell, 8 cell and blastocysts subjected to control and thawed conditions were analysed by semiquantitive reverse transcription polymerase chain reaction methods for hsp 70 mRNA expression. Results revealed the expression of hsp 70 mRNA were higher thawed embryos than control embryos. Immunocytochemistry used to localization the hsp70 protein in embryos. Two, 8-cell embryos derived under control condition was evenly distributed in the cytoplasm but appeared as aggregates in some embryos exposed frozen-thawed. However, under control condition, blastocysts displayed aggregate signal while Hsp70 in frozen-thawed blastocysts appeared to be more uniform in distribution.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.81-81
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• 2003

DNA methylation is a covalent modification of DNA that can modulate gene expression and is now recognized as a major component of the epigenome. During evolution, the dinucleotide CpG has been progressively eliminated from the genome of higher eukaryotes and is present at only 5% to 10% of its predicted frequency. Approxymately 80% of the remaining CpG sites contain methylated cytosines in most vertebrates and they are distributed in a pattern that is unique in each tissue and is inversely correlated with gene expression. The pattern of methylation is faithfully maintained during cell division by the enzyme Dnmt1, the maintenance DNA methyltransferase, which catalyzes the transfer of a methyl group from S-adenosyl-methionine to the 5'-position of the cytosine ring. We have been identified bovine Dnmt1 cDNA full-length recently (AY173048) Little is known on the functions of Dnmt1 in bovine preimplantation embryos. Thus, we analyzed the specific pattern of Dnmt1 in in vitro derived/nuclear transfer bovine and in vivo derived mouse embryos to monitor the epigenetic reprogramming process. We investigated these process by using indirect immunofluresence with an antibody to Dnmt1. According to other studies, Dnmt1 accumulates in nuclei of early growing oocytes but is sequestered in the cytoplasm of mature oocytes. In 2-cell and 4-cell embryos, Dnmt1 is cytoplasmic, but at the 8-cell stage, it is present only in the nucleus. By the blastocyst stage, Dnmt1o is again found only in the cytoplasm. Thus, nuclear localization of Dnmt1o in preimplantation embryos is limited to the 8-cell stages After implantation, Dnmt1 is localized in the nucleus in mouse. However, we have found different patterns of Dnmt1 nuclear localization. Though we used the common antibody, immune-localization data revealed that Dnmt1 antibody have been detected at the nucleus in 1-cell to blastocyst embryos. Therefore, maybe we think that the functions of Dnmt1 between bovine and mice are different. In order to Identify the mechanisms that regulate DNA methylation in bovine preimplantation embryo, we have plans on using bovine oocyte and somatic specific Dnmt1 antibodies.

• International conference on construction engineering and project management
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• 2020.12a
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• pp.267-277
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• 2020

The present research evaluates the public engagement approach in various project stages. Hong Kong had long been criticized as top-down and executive-led jurisdiction by overlooking the importance of cultural heritage and public concerns on public projects. It was suggested to the government to engage public and provide sufficient public consultation. Thereby, the government announced a series of revitalization and conversion measures in Policy Address in 2008. To carry out the measures, there were voices, because of diverse and sometimes conflicting interests, over the effect of revitalization project. On the other hand, studies reveal that there are benefits of revitalization and enhancement of public engagement approach. In pursuit of the subject, the present research aims at studying the Central Market as a case study pilot project. In October 2009, the Policy Address announced that the Central Market be revitalized. Tasked to implement the project, Urban Renewal Authority (URA) continued to adopt the people-oriented approach as the guiding principle in its core business and in heritage preservation and revitalization so as to create a sustainable development. Between government and the public, URA acts as a bridge for communication. As between URA and the public, URA conducted public consultation, set up an ad-hoc committee in January 2016 to be in charge of the project and will continuously inform the government and public with the updates and project progress. The main objectives of the present research are to assess the advantages and challenges in different stages of public engagement approach, to evaluate the engagement system, to give a comprehensive view for participation of stakeholders, and to find out effective strategies to enhance civil engagement. Research was achieved through interviews to key persons in the project, questionnaire that was distributed to community and experts in the field. Case Study of the Central Market was studied and investigated using different sources, such as newspapers, journals, etc, to evaluate the degree of public engagement in the project. Both detailed qualitative methodological approaches of interview, questionnaire, and case study, act as a synergy to demonstrate the research objectives and provide the comprehensive perceptions on the revitalization project. The results show that public participants in revitalized project have generated considerable value enhancements to social-cultural, environmental, political and economic aspect. This study provides valuable insights that the public participation can make positive contribution to sustainability in the city. The findings indicate that no any single system is flawless therefore seeking both public opinions and professional advices are also important as a comprehensive approach to achieve higher sustainability of the building.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.91-91
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• 2022

Popcorn grains are consumed around 10,000 tons per year, in South Korea. It is consumed in amusement parks, movie theaters and snacks. The size of the popcorn processed market in Korea is estimated to be 3.6 billon won per year. So, the popcorn grain market has good prospects. On the other hand, domestic grain is at the level of 1 %, which is less domestic production than the size of the market. Maize Research Institute has developed domestic varieties in order to increase the use of domestically produced grains. The Oyrunpopcorn variety which was commonly distributed is a preferred cultivar because it has a good popping rate compared to imported grains. In addition, 'G-Popcom', 'Oyrun #2' and 'Kichan Popcorn' were developed, which diversified the choice of the farmers. Yield per unit area is important to improve farmers' income. At present, domestic grain production is traded at 5,000 won/kg, so if the yield improves, a high farmer^ income is expected. Therefore, this study compared the growth characteristics and yield according to the appropriate sowing time and plant density to improve the yield of domestically grown popcorn. We used 'Oryunpopcorn', for this research. Agronomic characteristics were compared by sowing times April 23, May 22 and June 22. The 100 seeds weight were 15.9g, 17.7g and 15.0g, respectively. Kernel weight planted in May 22 is the highest value. Yield per 10a were 414kg, 434kg and 296kg, respectively. It shows the yield planted in May 22 was higher than other trials. Therefore, the sowing time to increase the kernel weight and yield is appropriate for planting in mid-May. The number of plants in planting density trial was 5,700 plants, 4,700 plants and 4,000 plants in 10a area. Plant height at each trial were 221 cm, 214cm and 218cm, respectively. It was the highest height in 5,700 plants trials. The 100 kernel weight were 14.8g, 15.9g and 16.5g, respectively. Low planting density trial indicated high kernel weight. Yield per 10a was 415kg, 357kg and 314kg, respectively. It was higher at high density trial than other experimental plots. Therefore, appropriate sowing time was in mid-May and planting density was 5,700 plants/10a in order to increase the yield of popcorn in South Korea. This study will be useful for farmer's income to use the domestic cultivars.

• Proceedings of the Korea Contents Association Conference
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• 2003.05a
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• pp.344-350
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• 2003

In this paper, we propose a novel sharing method ordering the events occurring between users collaborated with the common telecomputing environment. We realize the sharing method with multimedia data to improve the coworking effect using teleprocessing network. This sharing method advances the efficiency of communicating projects such as remote education, tele-conference, and co-authoring of multimedia contents by offering conveniences of presentation, group authoring, common management, and transient event productions of the users. As for the conventional sharing white board system, all the multimedia contents segments should be authored by the exclusive program, and we cannot use any existing contents or program. Moreover we suffer from the problem that ordering error occurs in the teleprocessing operation because we do not have any line-up technology for the input ordering of commands. Therefore we develop a method of retrieving input and output events from the windows system and the message hooking technology which transmits between programs in the operating system In addition, we realize the allocation technology of the processing results for all sharing users of the distributed computing environment without any error. Our sharing technology should contribute to improve the face-to-face coworking efficiency for multimedia contents authoring, common blackboard system in the area of remote educations, and presentation display in visual conference.

• Proceedings of the KOSOMBE Conference
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• v.1992 no.05
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• pp.27-47
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• 1992

Engineers have developed new instruments that aid in diagnosis and therapy Ultrasonic imaging has provided a nondamaging method of imaging internal organs. A complex transducer emits ultrasonic waves at many angles and reconstructs a map of internal anatomy and also velocities of blood in vessels. Fast computed tomography permits reconstruction of the 3-dimensional anatomy and perfusion of the heart at 20-Hz rates. Positron emission tomography uses certain isotopes that produce positrons that react with electrons to simultaneously emit two gamma rays in opposite directions. It locates the region of origin by using a ring of discrete scintillation detectors, each in electronic coincidence with an opposing detector. In magnetic resonance imaging, the patient is placed in a very strong magnetic field. The precessing of the hydrogen atoms is perturbed by an interrogating field to yield two-dimensional images of soft tissue having exceptional clarity. As an alternative to radiology image processing, film archiving, and retrieval, picture archiving and communication systems (PACS) are being implemented. Images from computed radiography, magnetic resonance imaging (MRI), nuclear medicine, and ultrasound are digitized, transmitted, and stored in computers for retrieval at distributed work stations. In electrical impedance tomography, electrodes are placed around the thorax. 50-kHz current is injected between two electrodes and voltages are measured on all other electrodes. A computer processes the data to yield an image of the resistivity of a 2-dimensional slice of the thorax. During fetal monitoring, a corkscrew electrode is screwed into the fetal scalp to measure the fetal electrocardiogram. Correlations with uterine contractions yield information on the status of the fetus during delivery To measure cardiac output by thermodilution, cold saline is injected into the right atrium. A thermistor in the right pulmonary artery yields temperature measurements, from which we can calculate cardiac output. In impedance cardiography, we measure the changes in electrical impedance as the heart ejects blood into the arteries. Motion artifacts are large, so signal averaging is useful during monitoring. An intraarterial blood gas monitoring system permits monitoring in real time. Light is sent down optical fibers inserted into the radial artery, where it is absorbed by dyes, which reemit the light at a different wavelength. The emitted light travels up optical fibers where an external instrument determines O2, CO2, and pH. Therapeutic devices include the electrosurgical unit. A high-frequency electric arc is drawn between the knife and the tissue. The arc cuts and the heat coagulates, thus preventing blood loss. Hyperthermia has demonstrated antitumor effects in patients in whom all conventional modes of therapy have failed. Methods of raising tumor temperature include focused ultrasound, radio-frequency power through needles, or microwaves. When the heart stops pumping, we use the defibrillator to restore normal pumping. A brief, high-current pulse through the heart synchronizes all cardiac fibers to restore normal rhythm. When the cardiac rhythm is too slow, we implant the cardiac pacemaker. An electrode within the heart stimulates the cardiac muscle to contract at the normal rate. When the cardiac valves are narrowed or leak, we implant an artificial valve. Silicone rubber and Teflon are used for biocompatibility. Artificial hearts powered by pneumatic hoses have been implanted in humans. However, the quality of life gradually degrades, and death ensues. When kidney stones develop, lithotripsy is used. A spark creates a pressure wave, which is focused on the stone and fragments it. The pieces pass out normally. When kidneys fail, the blood is cleansed during hemodialysis. Urea passes through a porous membrane to a dialysate bath to lower its concentration in the blood. The blind are able to read by scanning the Optacon with their fingertips. A camera scans letters and converts them to an array of vibrating pins. The deaf are able to hear using a cochlear implant. A microphone detects sound and divides it into frequency bands. 22 electrodes within the cochlea stimulate the acoustic the acoustic nerve to provide sound patterns. For those who have lost muscle function in the limbs, researchers are implanting electrodes to stimulate the muscle. Sensors in the legs and arms feed back signals to a computer that coordinates the stimulators to provide limb motion. For those with high spinal cord injury, a puff and sip switch can control a computer and permit the disabled person operate the computer and communicate with the outside world.