• 대한치과의사협회지
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• 제58권5호
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• pp.276-283
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• 2020

Objectives: The aim of this study was to evaluate the efficacy of CK files as an ultrasonic instrument, and to determine most efficient file size for smear layer removal. Materials and Methods: Thirty-six extracted human mandibular premolars with single, straight root canals and mature apices were mechanically prepared and randomly divided into three groups. Group 1 (Control) underwent conventional needle irrigation, Group 2 (CKS) underwent passive ultrasonic irrigation with a #20 CK file, and Group 3 (CKL) underwent passive ultrasonic irrigation with a #30 CK file. After preparation and irrigation, all teeth were dried and split with a chisel to obtain the mesial and distal half of their roots. Each sample was evaluated using a scanning electron microscope, and data were analyzed using the Kruskal-Wallis and Mann-Whitney rank sum tests (p<0.05). Results: The CKS group showed less debris in the apical third than the other groups (p<0.05). In this section, no significant difference was observed among the other groups. And, there was no significant difference among any groups for the middle third section. Conclusion: This study showed that PUI with #20 CK file removed more smear layer compared to using #30 CK file at the apical third of the root canal.

• Molecules and Cells
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• 제26권6호
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• pp.611-615
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• 2008

DNA methylation is an epigenetic mechanism for gene silencing. In Arabidopsis, MET1 is the primary DNA methyltransferase that maintains CG DNA methylation. Plants having an overall reduction of MET1 activity, caused by a met1 mutation or a constitutively expressed MET1 antisense gene, display genome hypomethylation, inappropriate gene and transposon transcription, and developmental abnormalities. However, the effect of a transient reduction in MET1 activity caused by inhibiting MET1 expression in a restricted set of cells is not known. For this reason, we generated transgenic plants with a MET1 antisense gene fused to the DEMETER (DME) promoter (DME:MET1 a/s). Here we show that DME is expressed in leaf primordia, lateral root primoridia, in the region distal to the primary root apical meristem, which are regions that include proliferating cells. Endogenous MET1 expression was normal in organs where the DME:MET1 a/s was not expressed. Although DME promoter is active only in a small set of cells, these plants displayed global developmental abnormalities. Moreover, centromeric repeats were hypomethylated. The developmental defects were accumulated by the generations. Thus, not maintaining CG methylation in a small population of proliferating cells flanking the meristems causes global developmental and epigenetic abnormalities that cannot be rescued by restoring MET1 activity. These results suggest that during plant development there is little or no short-term molecular memory for reestablishing certain patterns of CG methylation that are maintained by MET1. Thus, continuous MET1 activity in dividing cells is essential for proper patterns of CG DNA methylation and development.

• Archives of Reconstructive Microsurgery
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• 제19권1호
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• pp.46-49
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• 2010

Purpose: It has been reported that the ear perfusion can maintain by a very small pedicle because the ear has good vascularized system. Replantation of an amputated ear with vascular anastmosis, has been reported before and offers the succeessful reconstructive results. But, in this paper we report a case of complete nonmicrosurgical salvage of a nearly amputated ear based on 7 mm-wide small skin pedicle with adjunctive therapies. Methods: A 49-year-old man was referred with a nearly complete detachment of left ear. The blood supply to the ear was maintained exclusively on 7 mm-wide small skin pedicle in the lobule. After we identified the fresh bleeding at the distal margin of the detached ear, we performed the primary repair. At the end of the procedure, the areas of the concha bowl and helical root appeared to be congested. So the immediate postoperative treatment for improving the tissue survival was done with Lipo-Prostaglandin E1 (Eglandin$^{(R)}$) injection, leech apply and antibiotics medications. Results: Assessment of the replanted ear on postoperative day 14 revealed a nearly viable auricle including the helical root. The ear appeared to be entirely healed, with excellent projection and fully restored normal elasticity. Conclusion: We found the complete salvage of a nearly amputated ear based on 7 mm-wide small skin pedicle with adjunctive therapies including Lipo-Prostaglandin E1 (Eglandin$^{(R)}$) injection, leech apply and antibiotics without microsurgery.

• Journal of Periodontal and Implant Science
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• 제27권2호
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• pp.347-361
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• 1997

The present study investigates the effects of DFDB graft combined with Calcium sulfate membrane on the periodontal wound healing in dehiscence defects of dogs. Following the initiation of general anesthesia by I.V. administration of 30mg/kg of pentobarbital, first premolar was extracted and full-thickness flap was elevated from the second to the fourth premolar. The portion of premolars coronal to the alveolar crest was removed and mesial and distal roots separated to produce single rooted teeth. Exposed root canals were sealed with Caviton and covered completely with flaps sutured. Following the healing period of 12 weeks, the surgical sites were uncovered and $4{\times}4mm$ dehiscence defects were surgically created. Those defects with DFDB graft combined with Calcium sulfate membrane following root planing, were designated as test sites and those with flap surgery-only were designated as controls. 1. No foreign-body reaction or inflammation were observed in either groups. Calcium sulfate was completely resorbed in the test sites. 2. Significantly greater amounts of new cementum was observed in test sites compared with the controls. Significant amounts of functionally orientated collagens were observed in the test sites. 3. New bone formation was observed in significantly greater amounts in test sites. The results suggest that combined graft of DFDB and calcium sulfate is extremely biocompatible with a potential for new bone and cementum formation, and functional alignment of periodontal ligaments.

• Journal of Periodontal and Implant Science
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• 제27권3호
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• pp.585-595
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• 1997

Removal of subgingival calculus is essential for the success in periodontal treatment. Subgingival instrumentation is used for the removal of all bacterial plaque and calculus. In this study, two types of anterior curet were used on ant. teeth to conduct subgingival scaling and root planing. The remaining amount of calculus was evaluated according to type of instrument, depth of pocket, and tooth surface. 24 teeth extracted from patients being treated at Dan Kook University dept. Perio. were used. 4 surfaces per tooth a total of 96 areas were evaluated. 12 teeth treated with Gracey No. 1-2 was used as the control group and 12 teeth treated with Mini-five curet No. 1-2 was the experimental group. The 4 surfaces of the teeth {buccal, mesial, lingual or palatal, distal) were observed under a stereomicroscope and the images were captured 3 times per surfaced with a CCD. The image were observed on the monitor using a $10{\times]10$ grid produced with the Microsoft power point. The amount of calculus remaining was evaluated 3 times per surface. The results were as follows. 1. There was no significant difference in remaining calculus according to the pre-treatment pocket depth, and tooth position{Mx. or Mn). 2. The Mini-five curet showed better results than the Gracey curet but there was no statistically significant difference. 3. In both Gracey curet group and Mini-five curet group the lingual(or palatal) surface showed significant difference compared to the other surfaces(p<0.05). From the results above, it is thought that when treating ant. teeth consideration of the tooth surface is more important than the choice of instrument.

• 구강회복응용과학지
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• 제19권3호
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• pp.139-151
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• 2003

This study was to evaluate and to compare the compressive strength and the displacement effecting the abutment or the residual ridge which are transformed by the angle and the heights of the konus denture inner crown when restorating the unilateral konus denture by using the mandibular canine and the 1st premolar as an abutment. The author made 9 different models for different inner crown heights and konus angles. The inner crown height were divided to 5mm, 6mm, and 7mm and konus angles was divided to $4^{\circ}$, $6^{\circ}$, and $8^{\circ}$. And then in each model, 5kg of $15^{\circ}$ mesial load was stressed on the central fossa of the 1st premolar and the 1st molar. The stresses and displacement were measured using the finite element analysis. The results were as follows 1. The maximum compressive strength was shown on the connective area of the abutment and the denture base. 2. As the angle of the inner crown becomes increased, the compressive strength was shown smaller. 3. As the height of the inner crown becomes increased, the maximum compressive strength was shown smaller while the compressive strength of the root apex and the residual ridge showed larger. 4. When the stress was loaded only on the 1st premolar, the more compressive strength was concentrated on the root apex area of the 1st premolar. 5. When the stress was loaded only on the 1st premolar, the compressive strength was concentrated uniformly on the abutment and the residual ridge. 6. When the stress was loaded only on the 1st molar, the maximum displacement was shown on the distal part of the residual ridge.

• Restorative Dentistry and Endodontics
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• 제43권2호
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• pp.23.1-23.9
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• 2018

Objectives: This study evaluated the effect of ultrasonic agitation of mineral trioxide aggregate (MTA), calcium silicate-based cement (CSC), and Sealer 26 (S26) on adaptation at the cement/dentin interface and push-out bond strength. Materials and Methods: Sixty maxillary canines were divided into 6 groups (n = 10): MTA, S26, and CSC, with or without ultrasonic activation (US). After obturation, the apical portions of the teeth were sectioned, and retrograde cavities were prepared and filled with cement by hand condensation. In the US groups, the cement was activated for 60 seconds: 30 seconds in the mesio-distal direction and 30 seconds in the buccal-lingual direction, using a mini Irrisonic insert coupled with the ultrasound transducer. After the materials set, 1.5-mm thick sections were obtained from the apexes. The presence of gaps and the bond between cement and dentin were analyzed using low-vacuum scanning electron microscopy. Push-out bond strength was measured using a universal testing machine. Results: Ultrasonic agitation increased the interfacial adaptation of the cements. The S26 US group showed a higher adaptation value than MTA (p < 0.05). US improved the push-out bond strength for all the cements (p < 0.05). Conclusions: The US of retrograde filling cements enhanced the bond to the dentin wall of the root-end filling materials tested.

• Restorative Dentistry and Endodontics
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• 제47권1호
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• pp.1.1-1.13
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• 2022

Objectives: This study assessed the shape and anatomical relationship of the mental foramen (MF) to mandibular posterior teeth in an Indian sub-population. Materials and Methods: In total, 475 existing cone-beam computed tomography records exhibiting 950 MFs and including the bilateral presence of mandibular premolars and first molars were assessed. Images were evaluated 3-dimensionally to ascertain the position, shape, and anatomical proximity of MFs to mandibular teeth. The position and shape of MFs were measured and calculated. The Pythagorean theorem was used to calculate the distance between the root apex of the mandibular teeth and the MF. Results: MFs exhibited a predominantly round shape (left: 67% and right: 65%) followed by oval (left: 30% and right: 31%) in both males and females and in different age groups. The root apices of mandibular second premolars (left: 71% and right: 62%) were closest to the MF, followed by distal to the first premolars and mesial to the second premolars. The mean vertical distance between the MF and the nearest tooth apex calculated on sagittal sections was 2.20 mm on the right side and 2.32 mm on the left side; no significant difference was found according to sex or age. The distance between the apices of the teeth and the MF was ≥ 4 mm (left; 4.09 ± 1.27 mm and right; 4.01 ± 1.15 mm). Conclusions: These findings highlight the need for clinicians to be aware of the location of the MF in treatment planning and while performing non-surgical and surgical endodontic procedures.

• Journal of Chest Surgery
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• 제37권3호
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• pp.282-285
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• 2004

이상우쇄골하동맥은 약 0.5～2%에서 발생하며, 성인의 경우 대부분이 임상적인 증상 없이 생활하나 약 10%에서 기관이나 식도의 압박에 의한 임상증상을 나타내는 것으로 알려져 있다. 본 증례는 수년간 연하곤란 및 반복되는 폐렴을 주소로 한 이상우쇄골하동맥을 가진 64세 여자 환자를 보고한다. 혈관 촬영상 좌우 경동맥(carotid artery)이 하나의 동맥간(common trunk)에서 기시하고 우측 쇄골하동맥은 좌측 쇄골하동맥 기시부 가까이의 상행대동맥 후방에서 기시하여 식도의 후방으로 주행하는 이상 우쇄골하동맥을 진단하였다. 수술적 치료는 우측 개흉술을 통하여 이루어졌다. 식도의 후방부위를 박리하여 이상우쇄골하동맥을 완전히 박리하여 유동시켰다. 우쇄골하동맥을 박리한 후 근위부를 결찰하여 분리한 후 원위부를 인조혈관을 이용하여 대동맥 근위부와 연결하였다.

• 대한소아치과학회지
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• 제7권1호
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• pp.33-40
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• 1980

The author observed the eruption pattern of the mandibular premolars. For this study the author took 406 cases of oblique cephalographs of 6 to 12 year old children. The measurement were with regard to the vertical and horizontal dimensional changes and tooth axis changes to the occlusal plane. The following results were obtained. 1. At stage V the tooth axis of 1st premolar inclined mesially to the occlusal plane, as the tooth erupt toward vertically the angulation changed that at stage X the tooth axis almost right angle to the occlusal plane. 2. The distance from the root apex of premolars to the lower border of mandible were slightly increased to the stage VII, while after stage VII rapid increasing appeared. At stage X the distance of 1st premolar was more longer than 2nd premolar. 3. The distance from the mesial surface of 1st molar to the mesial surface of premolars were decreased slightly to the stage VII, but this distances were decreased rapidly after stage VII. 4. The distance between distal surface of 1st premolar and mesial surface of 2nd premolar was almost constant before stage VIII, at stage IX this distance was little presented because the premolars were contacted.