• Journal of Environmental Health Sciences
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• v.32 no.3
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• pp.227-234
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• 2006

There has been increasing concern in recent years about the occurrence, fate and toxicity of pharmaceutical products in aquatic environment. Although these compounds have been detected in a wide variety of environmental samples including sewage effluent, surface waters, ground water and drinking water, their concentrations generally range from the low ppt to ppb levels. It is therefore often thought to be unlikely that pharmaceuticals will have a detrimental effect on the environment. This study was conducted to determine the endocrine disruption effects of the several pharmaceutical residues in water using adult Japanese medaka (Oryzias latipes). The common antibiotics were used sulfa durgs (sulfamethoxazole and sulfamethazine) and tetracycline drugs (oxytetracycline and tetracycline). Positive control that was induced Vtg (vitellogenin) in male fish was used $17\beta$-estradiol. Vtg was qualified and quantified through Western blotting and ELISA. After SDS gel electrophoresis, the dominant protein band was identified to molecular weight approximately 205 kDa in whole body samples of vitellogenic female. In female medaka exposed to $17\beta$estradiol, there was no significant difference in total protein induction. In contrast, three to five day exposure of male fish to $17\beta$-estradiol resulted in more than 60.0% increase of total protein compared to that of control males (p<0.01). In case of antibiotics, female fish didn't show significant difference, but male fish was showed significant difference. In addition, Vtg induction in male fish was observed with all the test chemicals. On concentrations greater than 0.1 ppm of sulfamethoxazole, 1 ppm of sulfamethazine, 1 ppm of oxytetracycline and 20 ppm of tetracycline, Vtg induction was increased in a dose response manner. This study is one of the early reports suggesting potential endocrine disruption mechanism of antibiotic pharmaceutical products in aquatic ecosystem. Although the effect concentrations obtained from this study were high as unrealistically as in environments, it is endocrine disruption that we should be considered as one of the important consequences of pharmaceutical contamination at water environment, and warrants due attention in future researches.

• Biomolecules & Therapeutics
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• v.29 no.2
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• pp.175-183
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• 2021

The mitogen-activated protein kinase (MAPK) pathway controls intestinal epithelial barrier permeability by regulating tight junctions (TJs) and epithelial cells damage. Heme oxygenase-1 (HO-1) and carbon monoxide (CO) protect the intestinal epithelial barrier function, but the molecular mechanism is not yet clarified. MAPK activation and barrier permeability were studied using monolayers of Caco-2 cells treated with tissue necrosis factor α (TNF-α) transfected with FUGW-HO-1 or pLKO.1-sh-HO-1 plasmid. Intestinal mucosal barrier permeability and MAPK activation were also investigated using carbon tetrachloride (CCl4) administration with CoPP (a HO-1 inducer), ZnPP (a HO-1 inhibitor), CO releasing molecule 2 (CORM-2), or inactived-CORM-2-treated wild-type mice and mice with HO-1 deficiency in intestinal epithelial cells. TNF-α increased epithelial TJ disruption and cleaved caspase-3 expression, induced ERK, p38, and JNK phosphorylation. In addition, HO-1 blocked TNF-α-induced increase in epithelial TJs disruption, cleaved caspase-3 expression, as well as ERK, p38, and JNK phosphorylation in an HO-1-dependent manner. CoPP and CORM-2 directly ameliorated intestinal mucosal injury, attenuated TJ disruption and cleaved caspase-3 expression, and inhibited epithelial ERK, p38, and JNK phosphorylation after chronic CCl4 injection. Conversely, ZnPP completely reversed these effects. Furthermore, mice with intestinal epithelial HO-1 deficient exhibited a robust increase in mucosal TJs disruption, cleaved caspase-3 expression, and MAPKs activation as compared to the control group mice. These data demonstrated that HO-1-dependent MAPK signaling inhibition preserves the intestinal mucosal barrier integrity by abrogating TJ dysregulation and epithelial cell damage. The differential targeting of gut HO-1-MAPK axis leads to improved intestinal disease therapy.

• Management Science and Financial Engineering
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• v.8 no.1
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• pp.53-75
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• 2002

In this Paper, an attempt is made to establish a learning framework for robust planning and real-time execution control. Necessary definitions and concepts are clearly presented to describe real-time operational control in response to Plan disruptions. A general mathematical framework for disruption recovery is also laid out. Global disruption model is decomposed into suitable number of local disruption models. Execution Pattern is designed to capture local disruptions using decomposed-reverse neural mappings, and to further demonstrate how the decomposed-reverse mappings could be applied for solving disrubtion recovery problems. Two decomposed-reverse neural mappings, N-K-M and M-K-N are employed to produce transportation solutions in react-time. A potential extension is also discussed using the proposed mapping principle and other hybrid heuristics. Experimental results are provided to verify the proposed approach.

• Bulletin of the Korean Chemical Society
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• v.33 no.2
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• pp.426-432
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• 2012

The activity of an antimicrobial peptide, protegrin-1 (PG-1), on lipid membranes was investigated using solidstate NMR and a new sampling method that employed mechanically aligned bilayers between thin glass plates. At 95% hydration and full hydration, the peptide respectively disrupted 25% and 86% of the aligned 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphotidylcholine (POPC) bilayers at a P/L (peptide-to-lipid) ratio of 1/20 under the new experimental conditions. The kinetics of the POPC bilayers disruption appeared to be diffusioncontrolled. The presence of cholesterol at 95% hydration and full hydration reduced the peptide disruption of the aligned POPC bilayers to less than 10% and 35%, respectively. A comparison of the equilibrium states of heterogeneously and homogeneously mixed peptides and lipids demonstrated the importance of peptide binding to the biomembrane for whole membrane disruption.

• The Bulletin of The Korean Astronomical Society
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• v.38 no.2
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• pp.70.1-70.1
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• 2013

We present long-term optical to NIR data of the tidal disruption object, Swift J1644+57. The data were obtained with CQUEAN, UKIRT WFCAM observations. We analyze the morphology of the host galaxy of this object and decompose the bulge component using high resolution HST WFC3 images. We conclude that the host galaxy is bulge dominant. We also estimate the multi-band fluxes of the host galaxy through the light curves based on the long-term observational data. We fit the SED models to the multi-band fluxes of the host galaxy and determine its stellar mass. Finally, we estimate the mass of the central super massive black hole which is thought to be the main role of the tidal disruption event. The estimated stellar mass and black hole mass are $10^{9.1}M_{\odot}$, $10^{6.8}M_{\odot}$ respectively. We compare our results to other results that have studied before.

• BMB Reports
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• v.36 no.3
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• pp.265-268
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• 2003

Retinoic acid (RA) can transform the Golgi apparatus (GA) into a diffuse vacuolar aggregate and increase the toxicity of some immunotoxins that enter into cells by receptor-mediated endocytosis. An ultramorphological study of the RA-induced GA disruption was performed on F2000 fibroblasts. Cultures were treated with 0.11 to $30\;{\mu}M$ RA for 7 - 180 min. The endocytosis of Limax flavus agglutinin-peroxidase conjugate (LFA), and the interactions between a phorbol ester (PMA) and RA concerning GA disruption, were examined. Exposure to $0.33\;{\mu}M$ RA for 20 min transformed the GA into vacuolar aggregate. These vacuoles were not involved in endocytosis since they remained unstained after endocytosis of LFA. However, the lysosomes were involved in endocytosis, as they were strongly stained. Therefore, a RA-induced shift towards lysosomal routing of the entered LFA was presumed. Exposure to PMA made cells resistant to the Golgi-disturbing effects of RA, indicating that protein kinase C plays an important role in this process.

• Clinics in Shoulder and Elbow
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• v.8 no.2
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• pp.158-165
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• 2005

The Superior Shoulder Suspensory complex (SSSC) maintains a normal stable relationship between the upper extremity and the axial skeleton. Traumatic double disruptions of the SSSC frequently create an unstable anatomic situation and is difficult-to-treat. When this double disruption is managed conservatively, significant displacement can occur at either or both sites and result in long-term problems and functional disabilities. Therefore surgical management is generally necessary. The authors experienced two cases of double disruptions of the SSSC treated with arthroscopic surgery & and reported good results.

• Proceedings of the Korean Nuclear Society Conference
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• 1996.05d
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• pp.432-437
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• 1996

The observed characteristics of magnetic oscillations in L-H transition and disruption are described. Two kinds of MHD magnetic probes are used in order to cover broadband frequency range from 1.3 kHz to 300 kHz in the H-mode. Depending on the probe's position and frequency, different characteristics are observed. Precursor like oscillation in L-H transition, and the difference between sawtooth and ELM are discussed. All disruptions during the current rising phase are related with m＝2 or m＝3 mode. Different disruption characteristics for different operation conditions could be found in the MHD probes.

• Bulletin of the Korean Space Science Society
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• 2009.10a
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• pp.44.1-44.1
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• 2009

Identification of wave characteristics during current disruption events in the near-Earth geomagnetic tail region (~ 10 RE) is important to understand the substorm onset mechanism. In this paper, linear stability analysis in the ion-cyclotron grequency range, considering temperature anisotropy and cross-field flow is presented. It is found that the ion-cyclotron drift waves propagating in a quasi-perpendicular direction with respect to the ambient magnetic field are characterized by low frequencies ($\omega$ < $0.5{\Omega}ci$), while quasi-parallel waves have frequencies close to the ion-cyclotron frequency ($\omega{\sim}{\Omega}ci$). This finding is consistent with the observation by THEMIS spacecraft of a current disruption event in which a similar high- and low-frequency band structure is also present [A. T. Y. Lui, et al., J. Geophys. Res. 113, A00C06 (2008)]. It is also found that the quasi-perpendicular mode is excited by the ion cross-field flow.

• Environmental Engineering Research
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• v.11 no.3
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• pp.143-148
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• 2006

The ultrasonics is a new technology in waste activated sludge(WAS) treatment. Ultrasonic treatment is well known method for the break up of microbial cells to extract out a variety of intercellular materials inside microorganism cell. This study was done to investigate the effects of the ultrasonic frequency and power on disruption of biosolids in WAS and to examine the effect on methane production of WAS treated by ultrasonics. Biosolids disruption with ultrasound is more effective at ultrasonic frequency of 40 kHz and power of 0.3 watt/mL. In the digestion with WAS pretreated by sonication time for 10 minute at 40 kHz and 0.3 watt/mL, the total quantity of generated methane increased by 75%, as compared with experimental control(non-treatment).