• Title/Summary/Keyword: Disinfection

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Kinetic examination on synergistic effect of co-use of ultrasonic wave and ultraviolet radiation on bacterial disinfection in a household humidifier (초음파와 자외선(UV) 동시 적용이 가정용 가습기 내 살균에 미치는 시너지 효과에 대한 반응속도론적 연구)

  • Kim, Seunga;Kim, Jihyun;Lee, Minjoo;Kim, Jayun;Jung, Woosik;Park, Joonhong
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.3
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    • pp.227-235
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    • 2021
  • This study examined the effect of ultraviolet (UV) application on bacterial disinfection in a commercialized humidifier using ultrasonic wave (UW). To accurately examine disinfection kinetics in tap-water condition, tap-water was sterilized using a filter, and then inoculated with pure cultures of E. coli and P. putida with known viable counts. The disinfection kinetic characteristics were experimentally compared when UV alone, UW alone, and UW+UV together were applied in disinfecting the added bacteria in the commercialized humidifier. When UV alone was applied, bacterial disinfection kinetics followed a first-order decay reaction, and showed an approximately 10-time weaker disinfection compared to the typical UV disinfection in water treatment or wastewater treatment. When UW alone was applied, bacterial disinfection kinetics followed a second-order decay reaction with a low disinfection rate constant of 0.0002 min-1(CFU/mL)-1. When UV and UW were applied together, however and interestingly, the disinfection rate constant (0.0211 min-1(CFU/mL)-1) was approximately 100 times increased than that for the UW alone case. These results revealed that the co-use of UV and UW can provide synergistic effect on bacterial disinfection in a tap-water condition in household humidifiers.

Effect of Disinfection Process Combination on E. coli Deactivation and Oxidants Generation (E. coli 불활성화와 산화제 생성에 미치는 소독 공정 결합의 영향)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Science International
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    • v.20 no.7
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    • pp.891-898
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    • 2011
  • The aim of this research was to evaluate the effect of combination of disinfection process (electrolysis, UV process) on Escherichia coli (E. coli) disinfection and oxidants (OH radical, $ClO_2$, HOCl, $H_2O_2$ and $O_3$) generation. The effect of electrolyte type (NaCl, KCl and $Na_2SO_4$) on the E. coli disinfection and oxidants generation were evaluated. The experimental results showed that performance of E. coli disinfection of electrolysis and UV single process was similar. Combination of electrolysis and UV process enhanced the E. coli disinfection and 4-carboxybenzaldehyde (4-CBA, indicator of the generation of OH radical) degradation. It is clearly showed synergy effect on disinfection and OH radical formation. However chlorine ($ClO_2$, HOCl) and oxygen type ($H_2O_2$, $O_3$) oxidants were decreased with the combination of two process. In electrolysis + UV complex process, electro-generated $H_2O_2$ and $O_3$ were reacted with UV light of UV-C lamp and increased 4-CBA degradation(increase OH radical). Disinfection of electrolyte of chlorine type was higher than that of the sulfate type electrolyte due to the higher generation of OH radical and oxidants.

Kinetic analysis of E. coli disinfection using UV-LED (LED 광원 UV에 의한 대장균(E. coli) 소독의 속도론 해석)

  • Kim, Kyeong-Rae;Chang, In-Soung
    • Journal of Korean Society of Water and Wastewater
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    • v.35 no.6
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    • pp.489-496
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    • 2021
  • Water disinfection using UV-LED(Light emitting diode) has many advantages, such as smaller footprint and power consumption as well as relatively longer lifespan than those of conventional mercury-UV lamps. Moreover, UV-LED disinfection is considered an environmentally benign process due to its mercury-free nature. In this study, disinfection using an LED module emitting 275nm UV was carried out. 384 UV-LEDs were put into a cylinder tube with a capacity of 1.7 liters. The UV intensity of the UV-LED module was controlled from 1.7 to 8.4 mW/cm2. The disinfection efficiency for the model microorganism solutions(E. coli ) was monitored. As the UV intensity(I) and contact time(t) varied, inactivation of the microorganisms from 2 to 4-log-removals(i.e., 99 to 99.99% of disinfection efficiency) was achieved. Disinfection using UV-LED was followed to 1st order reaction and the reaction rate constant, k was determined. In addition, the relationship between UV intensity(I) and contact time(t) in order to obtain 99.99% of disinfection efficiency was modeled: I1.2·t= 460, which indicates that the product of UV intensity and contact time requiring 4-log-removals is always constant.

Inactivation of E. coli by Electrolysis+UV Process (전기+UV 공정에 의한 E. coli 불활성화)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Korean Society on Water Environment
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    • v.25 no.5
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    • pp.667-673
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    • 2009
  • This study has carried out to evaluate the performance of single (electrolysis, UV and ultrasonic process) and complex process (Electrolysis+UV, UV+Ultrasonic and Electrolysis+Ultrasonic) for the purpose of disinfection of Escherichia coli in water. The order of disinfection performance for E. coli in single process lie in: Electrolysis ${\fallingdotseq}$ UV >> ultrasonic process. OH radical was not produced in single disinfection process. Among the three kinds of complex process, disinfection performance of the Electrolysis+UV was higher than that of the other process (UV+Ultrasonic and Electrolysis+Ultrasonic). It demonstrated a synergetic effect between the UV and electrolysis. When the use of $Na_2SO_4$ as electrolyte instead of NaCl, current increase or more reaction time was needed for the complete disinfection. The disinfection performance of pre-electrolysis (20 W, 30sec) and post-UV (10 W, 30 sec) was higher than that of the simultaneous electrolysis+UV process at same electric power (30 W, 30 second).

A Study on the Microorganism Disinfection and Characteristics of Discharged Water of Dielectric Barrier Discharge Plasma Systems (유전체 장벽 방전 플라즈마 방전수의 특성과 미생물 소독에 관한 연구)

  • Kim, Dong-Seog;Park, Young-Seek
    • Journal of Environmental Health Sciences
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    • v.38 no.2
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    • pp.159-165
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    • 2012
  • Objectives: This experiment was carried out to elucidate the effect of discharged water on the disinfection of $Phytophthora$ $capsici$ and evaluate the water characteristics. Methods: The dielectric barrier discharges (DBD) plasma reactor system used in this study consisted of a plasma component [discharge, ground electrode and quartz dielectric tube], high voltage source, and air supply. The effects of water characteristics such as pH, ORP and conductivity and the disinfection effect of discharged water were investigated. Results: Experimental results showed that in the process of discharge, the pH decreased, whereas ORP and electric conductivity increased. When the discharge time was 30 min, $Phytophthora$ $capsici$ of 2.94 log was disinfected within 300 seconds. Disinfection performance of stored discharged water was maintained for three days; however the disinfection effect vanished after five days. When $Phytophthora$ $capsici$ was injected into the discharged water, the disinfection effect decreased after two days. Conclusions: It is considered that the main disinfection parameters of the discharged water were chemically active species such as $H_2O_2$ and $O_3$ and high ORP.

Disinfection of Water by Ultrasonic Irradiation (초음파 검사에 의한 수중의 살균처리)

  • 손종렬;유병성
    • Journal of environmental and Sanitary engineering
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    • v.14 no.1
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    • pp.54-61
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    • 1999
  • The industrial techniques of ultrasound have been used in the various fields, such as cleaning, medical surgery, emulsification, cell disruption etc. Especially the application of cell disruption was interested in the field of disinfection process in water by ultrasonic irradiation. It has been recognized that the ultrasounds are irradiated in aqueous solution, cavitation bubbles are generated and shock waves of high temperature and pressure are emitted as the bubbles are developed and finally broken, which function as a energy source to promote reaction efficiencies of various kinds of chemical reactions such as disinfection reaction in water. Therefore, this study was performed to apply the ultrasound for the disinfection method of infected drinking raw water and to discuss the limiting factors such as pH, sample volume and reaction temperature influenced on the removal efficiency of E. coli from experimental analysis of the results obtained in bench-scale plant. For the experiments to measure the influence of reaction parameters in the ultrasonic disinfection process, escalated reactivity of aqueous solutions was excellent when pH in aqueous solution was low, and sample volume was small. On the contrary, the reactivity of disinfection became elevated when reaction temperature was high. It was found that the rate constant of disinfection reaction was applied by Chick's law, reaction kinetics of Chick's law was irreversible and pseudo-first order at all the tested conditions.As a conclusion it appeared that the technology using ultrasonic irradiation can be applied to the treatment of disinfection in infected water which are difficult to be treated by conventional methods.

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Applying the Disinfecting Effects of Vinegar to Raw Vegetables in Foodservice Operations: A Focused Microbiological Quality Evaluation (급식소에서 이용되는 채소류의 식초수 소독의 적용을 위한 연구 - 미생물적 품질 평가를 중심으로 -)

  • Kim, Hyun-Jung;Kim, Heh-Young;Ko, Sung-Hee
    • Korean journal of food and cookery science
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    • v.23 no.4 s.100
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    • pp.567-578
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    • 2007
  • This study was designed to provide basic data for the application and practical use of vinegar disinfection for raw vegetable preparation in foodservice operations. The test materials were washed and disinfected by three different methods(tap water washing, chlorine water disinfection, vinegar disinfection) and stored at $3^{\circ}C$ and $10^{\circ}C$ for 7 days. Then, their microbiological qualities were compared. Total plate counts increased over the course of the storage period, and all samples, except in the case of tap water washing, remained under the permitted limit until the 7th day of storage. In particular, vinegar disinfection showed the lowest increase in total plate counts(2.35${\sim}$4.03 log CFU/g). While the total plate counts of chlorine and vinegar disinfection were within the 6.00 limit of microbiological safety, the tap water washing treatment passed the permitted limit. The psychrotrophic counts increased steadily over the course of the storage period, and increased least at $3^{\circ}C$, however, the tap water washing showed the most drastic increase, while vinegar disinfection yielded the smallest increase.

A Comparison of Single Disinfection Process for Inactivation of E. coli (E. coli 불활성화를 위한 단일 소독 공정의 비교)

  • Kim, Dong-Seog;Song, Seung-Koo;Park, Young-Seek
    • KSBB Journal
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    • v.25 no.1
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    • pp.25-32
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    • 2010
  • This study was carried out to evaluate the performance of three kinds of single process (electrolysis, UV and ultrasonic process) for the purpose of disinfection of Escherichia coli in water. Among the five kinds of electrode material, disinfection performance of Ir electrode was higher than that of the other electrodes. The order of disinfection performance for E. coli in single process lies in: electrolysis > UV $\gg$ ultrasonic process. Performance of the three single processes was increased with the increase of the electric power. Disinfection efficiency of the three processes was increased with the decrease of the pH. Disinfection of the UV process were decreased by the increase of NaCl dosage and air flow rate. However, ultrasonic process was not affected above two parameters. OH radical was not produced in UV and ultrasonic process. E. coli disinfection of the electrolysis process was well agreed with RNO degradation tendency, except pH.

Disinfection, Sterilization and Aseptic Technique for Thread Embedding Acupuncture (안전한 매선요법 시술을 위한 멸균, 소독 및 무균법)

  • Yun, Young-Hee;Son, Jae-Woong;Ko, Seong-Gyu;Choi, In-Hwa
    • The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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    • v.29 no.1
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    • pp.103-112
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    • 2016
  • Objective : Thread embedding acupuncture has become popular as a minimally invasive treatment for facial wrinkles and laxity. However, there is little published clinical practice guidelines about disinfection, sterilization and aseptic technique for thread embedding acupuncture. This study is to introducing a specific guidelines about disinfection, sterilization and aseptic technique for thread embedding acupuncture.Method : We reviewed internal regulations and guidelines about hospital infection, and Traditional Korean medicine doctors, nurses, and director of central supply room discussed in depth and established a regulation of disinfection, sterilization and aseptic technique for thread embedding acupuncture.Result : The regulation of disinfection, sterilization and aseptic technique for thread embedding acupuncture consisted of ① management of supplies, ② guidelines of disinfection, sterilization, and reuse, ③ aseptic technique for thread embedding acupuncture.Conclusion : Microbial management is an essential element of medical care and quality. Traditional Korean medicine doctors will care for disinfection, sterilization, and this should not neglect to comply with the procedures and guidelines in the medical field as well as to understand the aseptic techniques.

Disinfection Characteristics of Waterborne Pathogenic Protozoa Giardia lamblia

  • Kim, Kyongjoo;Wooksun Hong;Lee, Kisay
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.6 no.2
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    • pp.95-99
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    • 2001
  • Giardia lamblia is a parasitic protozoa which is transmitted in the form of a cyst through untreated water and also treated drinking water. Since its presence in water has led to frequent outbreaks of giardiasis and death in many countries, the removal and disinfection of this protozoan cyst from the water supply are of great concern for public health. This study examined the disinfection characteristics of G. lamblia cysts isolated from a Korean patient with giardiasis. When using sodium hypochlorite including 5 or 10 ppm chlorine, the killing rate was initially rapid, however, the disinfection slowed down and a 3log reduction could not be achieved even after 2h. The disinfection effectiveness was also reduced at a lower temperature, thereby implying that the risk o a giardiasis outbreak will be higher in the winter season. A CT (concentration$.$time) curve was constructed based on the results with sodium hypochlorite for use in designing and predicting disinfection performance. The organic chlorination disinfectant SDIS (sodium dichloroisocyanurate) produced a lower pH and a much higher residual effect than sodium hypochlorite. The disinfection of cysts by SDIC continued steadily throughout 2h of contact, although the initial killing rate was lower than that with sodium hypochlorite.

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