• Korean Journal of Environmental Biology
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• v.39 no.1
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• pp.1-5
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• 2021

Radish is one of the major vegetable crops and has been damaged economically by the causal agent of Fusarium wilt disease, Fusarium oxysporum f. sp. raphani (FO) in the highlands of Gangwon province, Korea. Recently, clover cyst nematode, Heterodera trifolii(HT), occurred in highland of Jeongseon area and poses a threat to radish plants. The nematodes, besides causing direct damage to radish plants, possess the capability to synergize with other plant pathogens, leading to the development of a disease complex. The aim of this study was to investigate the interaction between FO and HT in radish plants. The length of plants co-infected with both FO and HT was shorter than that of plants infected with FO alone, but there was no statistically significant difference. However, the disease severity was much higher in the plants co-infected with both FO and HT(1.60±0.97) compared to FO alone(0.20±0.42)(df=3, F=10.17, p<0.001). Disease incidence was also about two-fold higher in plants co-infected with both FO and HT (80%) compared to FO alone (44%). These results suggested that the clover cyst nematode should be considered in the management of Fusarium wilt disease in the highlands of Gangwon province.

• The Plant Pathology Journal
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• v.38 no.2
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• pp.146-158
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• 2022

Changes in physiological and biochemical patterns in lucerne plants caused by the presence of 'Candidatus Phytoplasma australasia', which is one of the significant pathogens causing yield losses in lucerne plants, were investigated. Significant differences were evident in total chlorophyll, chlorophyll a, chlorophyll b, and protein amounts between 'Ca. Phytoplasma australasia'-positive and negative lucerne plants. Stress-related metabolites such as phenol, malondialdehyde, and proline accumulations in 'Ca. Phytoplasma australasia'-positive plants were remarkably higher than those of phytoplasma-negative plants. As a response to disease attack, phytoplasma-positive plants exhibited higher antioxidant enzymes (peroxidase and catalase) and nonenzymatic metabolite responses such as jasmonic and salicylic acids. We state that partial disease responses were revealed for the first time to breed resistant lucerne lines infected by 'Ca. Phytoplasma australasia'.

• Korean Journal Plant Pathology
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• v.10 no.4
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• pp.292-296
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• 1994

Main tobacco diseases were surveyed on tobacco plants in the northern Kyeongbuk area to evaluate the effectiveness of current disease control techniques from 1993 to 1994. The major disease in seedlings was the anthracnose (Colletotrichum tabacum) but the mosaic (TMV) in adult plants. Wild fire (Psedomonas syringae pv. tabaci) and hollow stalk (Erwinia carotovora subsp. carotovora) were more severe in 1993 than those in 1994 because of more rain. Establishment of drain-outlet at the bottom of the temporary transplanting pot gave a significant reduction in anthracnose severity. Most farmers have ignored the protection effect of using milk during transplanting and the removal of diseased roots from the fields on mosaic (TMV). It was considered that the educating program on disease diagnosis and fungicide application might help farmers for the proper use of chemicals.

• Journal of Korean Neurosurgical Society
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• v.57 no.6
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• pp.440-444
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• 2015

Quality of life is the current trend and issue for the most of human diseases. In moyamoya disease (MMD), surgical revascularization has been recognized as the possible assistance to reduce the neurological insult. However, the progressive nature of the disease has been invincible so far. To improve the quality of life of MMD patients not only the protection from the neurological insult but also the maintenance or improvement of cognitive function is inevitable. For pediatric MMD patients, younger age or longer duration of disease is the key factor among the prognostic factors for bad neurological outcomes. Hence, 'the earlier, the better' is the most precious rule for treatment. Protection from neurological insult is very critical and foremost important to improve cognitive outcome. Clinicians need to know the neuropsychological profile of MMD patients for the care of whole person and make an effort to protect the patients from neurological insults to maintain or improve it.

• Journal of Radiation Protection and Research
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• v.48 no.3
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• pp.107-116
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• 2023

This article reviews the experience of applying the Identification, Diagnosis, Prospective, and Action (IDPA) facilitating method as a means of promoting practices of dialogue between stakeholders in the radiological protection field. After presenting the characteristics of the IDPA method and its ability to promote active listening, participation, and dialogue among stakeholders facing complex situations, as well as the procedural aspects associated with its practical implementation, the article describes three examples of the application of the method in the field of radiological protection. The first one presents how the IDPA method supported a debate among decision-makers, authorities, experts, professionals, and representatives of non-governmental organizations about how to engage stakeholders in radiological protection. The second example presents how the IDPA method was used in a series of dialogue meetings to explore the challenges of the post-nuclear accident situation resulting from the Fukushima Daiichi Nuclear Power Plant accident. The third one presents the application of the method in the context of a training course organized by Nagasaki University in the affected area close to the damaged plant. Experience has shown that the IDPA method makes it possible to develop responses to problems posed in very different contexts and, in many cases, to find compromises regarding their solutions. The IDPA method has the merit of allowing each of the participants to better understand the situation they are faced with, even if such a positive result is not always achieved.

• The Plant Pathology Journal
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• v.33 no.2
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• pp.184-192
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• 2017

Tylenchulus semipenetrans is an important and widespread plant-parasitic nematode of citrus worldwide and can cause citrus slow decline disease leading to significant reduction in tree growth and yield. Rapid and accurate detection of T. semipenetrans in soil is important for the disease forecasting and management. In this study, a loop-mediated isothermal amplification (LAMP) assay was developed to detect T. semipenetrans using DNA extracted from soil. A set of five primers was designed from the internal transcribed spacer region (ITS1) of rDNA, and was highly specific to T. semipenetrans. The LAMP reaction was performed at $63^{\circ}C$ for 60 min. The LAMP product was visualized directly in one reaction tube by adding SYBR Green I. The detection limit of the LAMP assay was $10^{-2}J2/0.5g$ of soil, which was 10 times more sensitive than conventional PCR ($10^{-1}J2/0.5g$ of soil). Examination of 24 field soil samples revealed that the LAMP assay was applicable to a range of soils infested naturally with T. semipenetrans, and the total assay time was less than 2.5 h. These results indicated that the developed LAMP assay is a simple, rapid, sensitive, specific and accurate technique for detection of T. semipenetrans in field soil, and contributes to the effective management of citrus slow decline disease.

• The Plant Pathology Journal
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• v.40 no.2
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• pp.160-170
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• 2024

Erwinia amylovora is a plant pathogen that causes fire blight on apples and pears. Bacteriophages, which are viruses that selectively infect specific species of bacteria and are harmless to animal cells, have been considered as biological control agents for the prevention of bacterial pathogens. In this study, we aimed to use bacteriophages that infect E. amylovora as biocontrol agents against fire blight. We isolated bacteriophages Fifi044 and Fifi318 infecting E. amylovora, and characterized their morphology, plaque form, and genetic diversity to use as cocktails for disease control. The stabilities of the two phages were investigated at various temperatures and pH values and under sunlight, and long-term storage experiment was conducted for a year. To evaluate whether the two phages were suitable for use in cocktail form, growth curves of E. amylovora were prepared after treating the bacterial cells with single phages and a phage cocktail. In addition, a disease control test was conducted using immature apples and in vitro cultured apple plantlets to determine the biocontrol effects of the phage cocktail. The two phages were morphologically and genetically different, and highly stable up to 50℃ and pH value from 4 to 10. The phages showed synergistic effect when used as a cocktail in the inhibition of host bacterial growth and the disease control. This study demonstrated that the potential of the phage cocktail as a biocontrol agent for commercial use.

• Research in Plant Disease
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• v.28 no.4
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• pp.209-220
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• 2022

Charcoal canker of oak, which has recently increased in southern Iran, could pose a serious threat to the entire forest ecosystem in the near future. In addition, it seems that climate change and its consequences, such as drought in the southern regions of Iran, have exacerbated this phenomenon. Consequently, the objective of this study was to identify the fungal pathogens that could cause charcoal canker disease in the oak forests of South Zagros. It was also sought to find associations between changes in the occurrence/exacerbation of charcoal canker disease under non and intense drought stress in non-inoculated or inoculated Quercus brantii seedlings. In total, 120 isolates were obtained from eight oak forests located in the Zagros Mountains of Southern Iran, Kohgiluyeh & Boyer-Ahmad and Fars provinces, which were classified as Biscogniauxia mediterranea based on morphological assessment. Subsequently, molecular assay confirmed the result by phylogenetic inference of internal transcribed spacer-rDNA regions, α-actin, and β-tubulin genes. The results of the pathogenicity test showed that the response of isolates of B. mediterranea (Iran-G1 and Iran-M70) was varied in different environments for the measured necrotic lesion length. In comparison with the control moisture treatments (non-stress), the necrotic lesion length in inoculated treatments increased under intense drought stress. In general, inoculated oak seedlings' exposure to water-deficient stress by the pathogen of B. mediterranea could affect the spread/severity of the charcoal canker disease.

• The Korean Journal of Mycology
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• v.52 no.1
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• pp.19-24
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• 2024

Rice sheath rot causes yield losses of up to 85% in rice-growing areas worldwide. In September 2023, the disease was detected in rice fields at the National Institute of Agricultural Sciences in Wanju, Korea. Two isolates (FD00214 and FD00215) were collected from symptomatic rice sheaths and identified as Sarocladium strictum based on their morphological characteristics and molecular sequencing of two genomic regions: the internal transcribed spacer and actin. Pathogenicity tests confirmed that S. strictum were the cause of the disease. To our knowledge, this is the first report of rice sheath rot caused by S. strictum in South Korea.

• The Plant Pathology Journal
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• v.28 no.4
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• pp.454-454
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• 2012