• 생물정신의학
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• 제8권2호
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• pp.203-207
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• 2001

The genome sequencing project has generated and will continue to generate enormous amounts of sequence data including 5 eukaryotic and about 60 prokaryotic genomes. Given this ever-increasing amounts of sequence information, new strategies are necessary to efficiently pursue the next phase of the genome project-the elucidation of gene expression patterns and gene product function on a whole genome scale. In order to assign functional information to the genome sequence, DNA chip(or gene microarray) technology was developed to efficiently identify the differential expression pattern of independent biological samples. DNA chip provides a new tool for genome expression analysis that may revolutionize many aspects of biotechnology including new drug discovery and disease diagnostics.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권3호
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• pp.159-163
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• 2000

The genome sequencing project has generated and will contitute to generate enormous amounts of sequence data. Since the first complete genome sequence of bacterium Haemophilus in fluenzae was published in 1995, the complete genome sequences of 2 eukaryotic and about 22 prokaryotic organisms have detemined. Given this everincreasing amounts of sequence information, new strategies are necessary to efficiently pursue the phase of the geome project- the elucidation of gene expression patterns and gene product function on a whole genome scale. In order to assign functional information to the genome sequence, DNA chip technology was developed to efficienfly identify the differential expression pattern of indepondent biogical samples. DNA chip provides a new tool for genome expreesion analysis that may revolutionize revolutionize many aspects of human kife including mew surg discovery and human disease diagnostics.

• 한국의사학회지
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• 제17권2호
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• pp.45-62
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• 2004

In this paper, we will take a look at the perception of gynecological diseases in the JinYuanSiDaJia (金元四大家-the four great schools of Jin Yuan), including the academic schools LiuWanSu(劉完素), ZhangCongZheng(張從正), LiGao, and ZhuZhenheng(朱震亨) during JinYuan Dynasty(金元時代). We also examined the medical history of gynecology up until the MingQingDynasty(明淸時代) when the dialectical system on gynecological diseases was relatively complete.

• 전자통신동향분석
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• 제35권2호
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• pp.1-16
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• 2020

"Artificial Intelligence+" is a key strategic direction that has garnered the attention of several global medical device manufacturers and internet companies. Large hospitals are actively involved in different types of medical AI research and cooperation projects. Medical AI is expected to create numerous opportunities and advancements in areas such as medical imaging, computer aided diagnostics and clinical decision support, new drug development, personal healthcare, pathology analysis, and genetic disease prediction. On the contrary, some studies on the limitations and problems in current conditions such as lack of clinical validation, difficulty in performance comparison, lack of interoperability, adversarial attacks, and computational manipulations are being published. Overall, the medical AI field is in a paradigm shift. Regarding international standardization, the work on the top 10 standardization issues is witnessing rapid progress and the competition for standard development has become fierce.

• Parasites, Hosts and Diseases
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• 제53권5호
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• pp.575-581
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• 2015

Malaria is a tropical disease caused by protozoans of the Plasmodium genus. Delayed diagnosis and misdiagnosis are strongly associated with higher mortality. In recent years, a greater importance is attributed to Plasmodium knowlesi, a species found mainly in Southeast Asia. Routine parasitological diagnostics are associated with certain limitations and difficulties in unambiguous determination of the parasite species based only on microscopic image. Recently, molecular techniques have been increasingly used for predictive diagnosis. The aim of the study is to draw attention to the risk of travelling to knowlesi malaria endemic areas and to raise awareness among personnel involved in the therapeutic process.

• Journal of Microbiology and Biotechnology
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• 제32권1호
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• pp.1-5
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• 2022

Diagnostics, vaccines, and drugs are indispensable tools and control measures employed to overcome infectious diseases such as coronavirus disease 2019 (COVID-19). Diagnostic tools based on RT-PCR were developed early in the COVID-19 pandemic and were urgently required for quarantine (testing, tracing and isolation). Vaccines such as mRNA vaccines and virus-vectored vaccines were also successfully developed using new platform technologies within one year after identifying severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as the causative agent of COVID-19. Drug development has been conducted in various ways including drug repurposing, convalescent plasma therapy, and monoclonal antibody development. Among the above efforts, this review examines COVID-19 drug development along with the related and upcoming challenges.

• 센서학회지
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• 제32권1호
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• pp.1-9
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• 2023

Recently, the digital healthcare technologies including non-invasive diagnostics based on Internet of Things (IOT) are getting attention. Human exhaled breath contains a variety of volatile organic compounds (VOCs), which can provide information of malfunctions of the body and presence of a specific disease. Detection of VOCs in exhaled breath using gas sensors are easy to use, safe, and cost-effective. However, accurate diagnosis of diseases is challenging because changes in concentration of VOCs are extremely small and lots of body factors directly or indirectly influence to the conditions. To overcome the limitations, highly selective nanosensors and artificial intelligent electronic nose (E-nose) systems have been mainly researched in recent decades. This review provides brief reviews of the recent studies for diabetes and lung cancer diagnosis using nanosensors and E-nose systems.

• 동국한의학연구소논문집
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• 제8권2호
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• pp.155-173
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• 2000

한의학의 진단(診斷)에는 망(望) 문(聞) 문(問) 절(切)의 사진법(四診法)과 여러 가지의 변증체계(辨證體系), 즉 기혈진액변증(氣血津液辨證) 장부변증(臟腑辨證), 육경변증(六經辨證), 위기영혈변증(衛氣營血辨證), 삼초변증(三焦辨證), 사상체질변증(四象體質辨證) 등이 응용되어 지고 있으며, 또한 그와 더불어 병상(症狀) 증후감병진단법(證候鑑別診斷法)등이 선택적으로 활용되어 지고 있다. 이러한 광범위(廣範位)한 진단방법(診斷方法)가운데 절진(切診)은 손가락 및 손바닥의 감각(感覺)을 운용(運用)해서 일정부위(一定部位)를 촉지(觸指), 접압(接壓)하는 검사방법(檢査方法)으로써 절맥진(切脈診)과 접진(接診)으로 크게 나눌 수 있다. 이중 안진(按診)이란 손을 사용하여 직접 환부에 촉모(觸摸) 안압(按壓)하여 이상변화를 알아내고 나아가서는 질병(疾病)의 부위(部位)와 성질(性質)과 병정(病情)의 경중(輕重) 등의 내부(內部)의 변화(變化)와 체표(體表)의 반응(反應)을 관찰(觀察)하여 중요(重要)한 변증자료(辨證資料)를 얻는 진단방법(診斷方法)의 한 종류(種類)를 말한다. 또한 접진(接診)에는 안기표(按肌表), 접수족(接手足), 안흉복(按胸腹), 접유혈진법(接兪穴診法)등을 들 수 있다. 배유혈(背兪穴)의 진단법(診斷法)은 경기(經綺)이라는 반응로(反應路)를 통(通)하여 체표(體表)에 발현(發現)되는 압통(壓痛), 자발통(自發痛), 긴장(緊張), 이완(弛緩), 경결(硬結) 및 조색상물(條索狀物) 등의 현상(現象)으로 부터 내부장기(內部臟器)의 병변(病變)을 진단(診斷)하는 방법(方法)이다. 이에 저자(著者)는 접진(接診)의 내용(內容)과 방법(方法)을 연구하면서 십이경맥(十二經脈)의 시동병(始動病) 소생병(所生病)을 알아보고 혈위진단(穴位診斷)의 방법(方法) 및 주의점(注意點)등을 아울러 정리하므로써 다음과 같은 결론(結論)을 얻었다. 1. 유혈(兪穴)은 각(各) 장부(臟腑)의 사기(邪氣)가 주입(注入)하는 곳으로 장병(臟病) 한증(寒症) 허증(虛症)의 의미를 내포한 음성병증(陰性病症) 치료(治療)에 중요(重要)한 곳이다. 2. 유차(兪次)의 촉진(觸診) 즉(卽) 모지(母指)로서 척추극돌기(脊椎棘突起) 좌우측(左右側)을 접압(接壓)하여서 상향(上向)이나 하향(下向)으로 추압지(推壓指)하면 극돌기(棘突起)의 돌(突), 함요(陷凹), 긴장(緊張), 이완(弛緩) 및 압통(壓痛)의 출현부위(出現部位)에 따라 계통별(系統別) 질환(疾患)을 판단(判斷)할 수 있다. 3. 실제(實際) 임상(臨床)에서 환자(患者)의 진단(診斷) 치료(治療)에 있어서 배부접진(背部接診)은 중요(重要)한 진단(診斷)의 한 영역(領域)으로 빠뜨리지 말고 꼭 참고(參考)하여야 할 것으로 사료(思料)된다. 4. 장부질환(臟腑疾患)에 대한 진단방법(診斷方法)의 다양화(多樣化)와 치료영역(治療領域)의 확대(擴大) 및 치료율(治療率)의 상승(上昇)을 위해 배부유혈(背部兪穴)의 정확(正確)한 인식(認識)과 유혈접진(兪穴接診)을 통하여 정확(正確)한 진단(診斷)이 되었으면 한다.

• 한국방사선학회논문지
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• 제17권3호
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• pp.385-392
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• 2023

카롤리병은 섬유낭성 간질환이다. 상염색체 열성 장애로 담관의 선천적 다발성 확장이 특징이다. 카롤리병을 진단하는 방법으로 전산화단층촬영, 자기공명영상 검사, 담관 조영술, 초음파 검사가 있다. 전산화단층촬영검사는 섬유다낭성 간 질환을 감지하고 구별하는데 필수적이며, 간 내 담관의 확장을 판별하는 데 유용하다. 하지만 조영제를 사용함으로써 일어날 수 있는 부작용에 대한 인지가 필요하다. 자기공명영상검사는 대표적으로 자기공명담췌관조영술 방법을 이용한다. 비침습적 검사로 환자의 고통을 경감할 수 있고, 담췌관부의 해부학적 구조와 병변 유무를 쉽고 빠르게 관찰이 가능하다. 담관 조영술은 비대해진 담관 전체에 걸친 여러 낭성 확장을 직접 시각화할 수 있는 효과적인 진단 방법이다. 하지만 이 시술 또한 침습적인 시술이므로 진단이 아닌 치료 목적으로 권장된다. 초음파 검사는 전산화단층촬영과 유사한 소견을 확인할 수 있다. 간동맥 뿌리는 기존의 그레이 스케일 초음파에서 증명하기 쉽지 않다. 하지만 관 내 혈관 뿌리를 가진 확장된 담관을 묘사할 수 있을 뿐만 아니라 관 내 색상 도플러 신호를 쉽게 식별할 수 있다는 점에서 임상적 가치를 갖고 있다. 최근엔 영상진단의 발전으로 전산화단층촬영 검사, 자기공명영상 검사, 담관 조영술, 초음파 검사 등으로 조기 진단이 가능해졌다. 조기 진단을 통해 치료 후에 장기적인 예후가 개선될 수 있도록 영상 진단 발전에 더욱 기여하고자 각 검사별 카롤리병에 대하여 어떠한 양상이 나타나는지 고찰하였다

• 동의생리병리학회지
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• 제20권6호
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• pp.1636-1648
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• 2006

There are detailed descriptions of the clinical experiences and prescriptions of asthma in traditional Korean medicine. Zedoariae rhizoma is one of the Korean herbal medicines used to treat bronchial asthma and allergic rhinitis for centuries. However, the therapeutic mechanisms of this medication are still far from clear, In this study, a house-dust-mite (Dermatophagoides pteronyssinus [Der p])-sensitized murine model of asthma was used to evaluate the immunomodulatory effect of Zedoariae rhizoma on the allergen-induced airway inflammation in asthma. Three different protocols were designed to evaluate the treatment and/or long-term prophylacitic effect of Zedoariae rhizoma in Der p-sensitized mice. Cellular infiltration and T-cell subsets in the bronchoalveolar lavage fluid (BALF)of allergen-challenged mice were analyzed. Intrapulmonary lymphocytes were also isolated to evaluate their response to allergen stimulation. When Zedoariae rhizoma was administered to the sensitized mice before AC (groups A and C), it suppressed airway inflammation by decreasing the number of total cells and eosinophil infiltration in the BALF, and downregulated the allergen- or mitogen-induced intrapulmonary lymphocyte response of sensitized mice as compared to those of controls. This immunomodulatory effect of Zedoariae rhizoma may be exerted through the regulation of T-cell subsets by elevation or activation of the CD8+ and double-negative T-cell population in the lung. However, the administration of Zedoariae rhizoma to sensitized mice 24 h after AC (group B) did not have the same inhibitory effect on the airway inflammation as Zedoariae rhizoma given before AC. Thus, the administration of Zedoariae rhizoma before AC has the immunomodulatory effect of reducing bronchial inflammation in the allergen-sensitized mice. On the other hand, to determine the potentiality of prophylactic and/or therapeutic approaches using a traditional herbal medicine, Zedoariae rhizoma, for the control of allergic disease, we examined the effects of oral administration of Zedoariae rhizoma on a murine model of asthma allergic responses. When oral administration of Zedoariae rhizoma was begun at the induction phase immediately after OVA sensitization, eosinophilia and Th2-type cytokine production in the airway were reduced in OVA-sensitized mice following OVA inhalation. These results suggest that the oral administration of Zedoariae rhizoma dichotomously modulates allergic inflammation in murine model for asthma, thus offering a different approach for the treatment of allergic disorders.