• Journal of Microbiology and Biotechnology
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• 제31권3호
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• pp.358-367
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• 2021

The World Health Organization (WHO) has declared the coronavirus disease 2019 (COVID-19) as an international health emergency. Current diagnostic tests are based on the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) method, which is the gold standard test that involves the amplification of viral RNA. However, the RT-qPCR assay has limitations in terms of sensitivity and quantification. In this study, we tested both qPCR and droplet digital PCR (ddPCR) to detect low amounts of viral RNA. The cycle threshold (CT) of the viral RNA by RT-PCR significantly varied according to the sequences of the primer and probe sets with in vitro transcript (IVT) RNA or viral RNA as templates, whereas the copy number of the viral RNA by ddPCR was effectively quantified with IVT RNA, cultured viral RNA, and RNA from clinical samples. Furthermore, the clinical samples were assayed via both methods, and the sensitivity of the ddPCR was determined to be equal to or more than that of the RT-qPCR. However, the ddPCR assay is more suitable for determining the copy number of reference materials. These findings suggest that the qPCR assay with the ddPCR defined reference materials could be used as a highly sensitive and compatible diagnostic method for viral RNA detection.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.259-271
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• 2021

Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural environment over those with only a single copy. In the naphthalene-degrading strain Pseudomonas putida ND6, catechol can be converted into intermediates of the tricarboxylic acid cycle via either the ortho- or meta-cleavage pathways. In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catBICIAI and catBIICIIAII) on the chromosome play an important role. The catI and catII operons are co-transcribed, whereas catAI and catAII are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. In the presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatRI and CatRII occupy an additional downstream site, designated as the activation binding site. Notably, CatRI binds to both the catI and catII promoters with high affinity, while CatRII binds weakly. This is likely caused by a T to G mutation in the G/T-N11-A motif. Specifically, we found that CatRI and CatRII regulate catBICIAI and catBIICIIAII in a cooperative manner, which provides new insights into naphthalene degradation.

• The Korean Journal of Physiology and Pharmacology
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• 제26권4호
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• pp.263-275
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• 2022

There is a paucity of detailed data related to the effect of senescence on the mitochondrial antioxidant capacity and redox state of senescent human cells. Activities of TCA cycle enzymes, respiratory chain complexes, hydrogen peroxide (H₂O₂), superoxide anions (SA), lipid peroxides (LPO), protein carbonyl content (PCC), thioredoxin reductase 2 (TrxR2), superoxide dismutase 2 (SOD2), glutathione peroxidase 1 (GPx1), glutathione reductase (GR), reduced glutathione (GSH), and oxidized glutathione (GSSG), along with levels of nicotinamide cofactors and ATP content were measured in young and senescent human foreskin fibroblasts. Primary and senescent cultures were biochemically identified by monitoring the augmented cellular activities of key glycolytic enzymes including phosphofructokinase, lactate dehydrogenase, and glycogen phosphorylase, and accumulation of H₂O₂, SA, LPO, PCC, and GSSG. Citrate synthase, aconitase, α-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, isocitrate dehydrogenase, and complex I-III, II-III, and IV activities were significantly diminished in P25 and P35 cells compared to P5 cells. This was accompanied by significant accumulation of mitochondrial H₂O₂, SA, LPO, and PCC, along with increased transcriptional and enzymatic activities of TrxR2, SOD2, GPx1, and GR. Notably, the GSH/GSSG ratio was significantly reduced whereas NAD⁺/NADH and NADP⁺/NADPH ratios were significantly elevated. Metabolic exhaustion was also evident in senescent cells underscored by the severely diminished ATP/ADP ratio. Profound oxidative stress may contribute, at least in part, to senescence pointing at a potential protective role of antioxidants in aging-associated disease.

• 한국노년학
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• 제40권4호
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• pp.659-689
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• 2020

본 연구는 연령차별을 경험한 노인들이 스스로 부정적 차별 인식을 내면화하는 일련의 과정에 집중하는 연구이다. 노인들이 일상생활에서 경험하는 연령차별의 모습을 규명하고 이같은 차별경험이 '자기연령주의(self-ageism)'를 통해서 어떤 결과로 이어지는지를 탐색하기 위해 근거이론방법을 활용하였다. 분석결과에 따르면 노인들은 명시적 차별 뿐 아니라 암묵적 차별에도 반응하는데 이 과정에서 노인의 고통(빈곤, 질병, 무위, 고독)의 강도가 높고 객관적인 차별상황에 속에서 차별대응에 실패한 기억이 강할수록 더욱 높은 수준의 자기연령주의를 갖게 되는 것으로 나타났다. '자기연령주의'는 나이에 의한 '차별'을 당한 노인들이 스스로에 대한 부정적 이미지를 내재화함으로써, 현실에서 다양한 거부/분리/멈춤 등의 행동을 통해 삶의 질적 측면에서의 불이익을 감수하는 현상이다. 건강, 제도적 지원, 보듬어주는 사람들과 같은 지지적 자원들이 존재하는 경우에 노인들은 보다 적극적인 방식으로 작용/상호작용을 통해 자기연령주의를 극복할 여지를 보이지만, 반면 이러한 자원들의 부족으로 스스로 동기부여하지 못하는 노인들의 경우 자기연령주의로 인한 부정적 환류에 갇혀버리게 됨으로서 침체되고 위축된 삶을 살아가게 된다. 이런 상태에서 스스로를 동기화(motivation)해서 신체, 인지, 정서, 사회적 반응을 해나가는 것은 매우 쉽지 않은 일이다. 그러므로 분석과정에서 특별히 두 가지 부분, 맥락적 조건과 중재적 조건에 주목하며 미시적으로는 노인의 동기화와 회복력(resilience) 향상을 위한 프로그램을 제안하였고, 거시적으로는 전 생애주기와 관련한 복지 및 건강서비스 체계 보완, 커뮤니티케어를 통한 접근성과 통합성 확대, 인식개선과 차별금지법과 같은 시스템 마련의 필요성을 주장하였다.

• Journal of Nutrition and Health
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• 제55권4호
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• pp.419-429
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• 2022

수분은 인체에서 가장 많은 비율을 차지하는 성분으로 인체의 원활한 생리작용을 위해 필수적인 요소이다. 2013-2017 국민건강영양조사 자료를 근거로 하였을 때 우리나라 사람들의 평균 1일 수분 섭취량은 2,167.3 mL/day이며 그 중 62%가 섭취기준을 충족하지 못하고 있다. 반면 음료 섭취는 계속해서 증가하고 있는 추세다. 수분 섭취기준은 충분섭취량으로 제시되며, 상한섭취량과 만성질환위험감소섭취량은 제시하지 않고 있다. 2015년과 비교해 2020년 수분 충분섭취량은 연령에 따라 소폭 증가하거나 감소되었는데 유아기 1-2세, 남자 6-8세, 9-11세, 여아 6-8세는 100 mL/day씩 감소하였으며, 남자 12-14세는 100 mL/day 증가하였다. 수분 섭취기준으로 제시되는 수분 충분섭취량은 음식 수분 섭취량과 액체 수분 섭취량을 합한 값이며, 액체 수분 섭취량은 물 섭취량 중앙값, 음료 섭취량 중앙값, 우유 섭취량 200 mL/day을 합해서 산출한 수치이다. 이와 같이 수분 섭취기준에는 음식 수분 섭취량도 포함되어 있으므로, 물과 음료의 섭취기준으로 총수분 섭취기준을 적용하는 것은 적절하지 않으며 액체 섭취기준을 적용해야 한다. 그리고 액체 수분을 섭취할 때에는 당류, 카페인 등이 함유된 음료보다 물이나 우유를 섭취하는 것이 바람직하다. 한국인 수분 섭취기준 설정에 있어서 향후 개선하고 보완해야 할 사항으로 한국인 일상식에서 수분 함량비의 정확성 제고, 액체 수분 섭취량에 관한 조사방법 보완, 생애주기별 수분 섭취 실태에 대한 다각적인 검토, 노인기의 생리적 변화와 건강상태 반영, 한국인 대상 수분 섭취와 건강에 관한 연구 활성화와 반영 등을 제안한다.

• Journal of Digestive Cancer Research
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• 제5권2호
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• pp.113-119
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• 2017

Background: Second line chemotherapy is often considered in advanced gastric cancers. We assessed irinotecan in combination with fluorouracil in patients experienced diseases progression after first line chemotherapy. Methods: Prospective trial was done at 7 centers in republic of Korea. Patients aged 18 years or older with advanced gastric adenocarcinoma and disease progression on or within 4 months after first-line chemotherapy were assigned to receive irinotecan 180 mg/m² and 5-fluorouraicl 400 mg/m² intravenously bolus injection on days 1 and leucovorin 200 mg/m² for 2 hours and 5-fluorouracil 600 mg/m² for 22 hours intravenously infusion on day 2 of a 14-day cycle (FOLFIRI group). The primary endpoint was objective tumor response (OR). Efficacy analysis was by per-protocol, and safety analysis included all patients who received at least one treatment with study drug. Results: Between January 1, 2014 and December 31, 2016, 28 patients were assigned to FOLFIRI treatment. Of those 20 patients were completed the study protocol. Per-protocol analysis, two patients among 20 subjects (10.0%) showed partial response. Overall survivals of FOLFIRI group; median 10.1 months [95% CI 4.9-15.3] Grade 3 and higher adverse event that occurred about 5%, but grade 3 or higher febrile neutropenia or life threatening complication was not reported. Conclusion: Combination chemotherapy with irinotecan, 5-FU, and LV is feasible in gastric cancer patients previously treated with platinum-based chemotherapy

• Korean Journal of Radiology
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• 제21권4호
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• pp.471-482
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• 2020

Objective: We aimed to determine the optimized image-based surrogate endpoints (IBSEs) in targeted therapies for glioblastoma through a systematic review and meta-analysis of phase III randomized controlled trials (RCTs). Materials and Methods: A systematic search of OVID-MEDLINE and EMBASE for phase III RCTs on glioblastoma was performed in December 2017. Data on overall survival (OS) and IBSEs, including progression-free survival (PFS), 6-month PFS (6moPFS), 12-month PFS (12moPFS), median PFS, and objective response rate (ORR) were extracted. Weighted linear regression analysis for the hazard ratio for OS and the hazard ratios or odds ratios for IBSEs was performed. The associations between IBSEs and OS were evaluated. Subgroup analyses according to disease stage (newly diagnosed glioblastoma versus recurrent glioblastoma), types of test treatment, and types of response assessment criteria were performed. Results: Twenty-three phase III RCTs published between 2000 and 2017, including 8387 patients, met the inclusion criteria. OS showed strong correlations with PFS (standardized β coefficient [R] = 0.719), 6moPFS (R = 0.647), and 12moPFS (R = 0.638). OS showed no correlations with median PFS and ORR. In subgroup analysis according to types of therapies, PFS showed the highest correlations with OS in targeted therapies for cell cycle pathways (R = 0.913) and growth factor receptors and their downstream pathways (R = 0.962). 12moPFS showed the highest correlation with OS in antiangiogenic therapy (R = 0.821). The response assessment in neuro-oncology criteria provided higher correlation coefficients between OS and IBSEs than the Macdonald criteria. Conclusion: Overall, PFS is an optimized IBSE in targeted therapies for glioblastoma; however, 12moPFS is optimal in antiangiogenic therapy.

• 생명과학회지
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• 제34권6호
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• pp.399-407
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• 2024

혈관신생은 기존 혈관에서 새로운 혈관을 형성하는 과정이며, 이 현상은 성장, 치유, 월경주기의 변화 중에 발생한다. 종양의 경우, 혈관신생은 원발성 종양의 지속적인 성장, 전이 촉진, 전이성 종양 성장 지원 및 암 진행에 중요한 복잡하고 다면적인 과정이다. 혈관신생 장애는 암 발병, 자가면역 질환, 류마티스 관절염, 심혈관 질환 및 상처 치유 지연을 초래할 수 있다. 현재 유효한 혈관신생 억제약물은 제한된 수에 불과하다. 최근 연구에 따르면 해양 천연물이 혈관신생 억제효과를 나타내는 것으로 보고되고 있다. 이전 연구에서 Hizikia fusiformis의 핵산 추출물(HFC)이 in vitro 및 in vivo에서 혈관 신생 억제효과를 확인하고 보고하였다. 본 연구의 목적은 H. fusiformis의 클로로포름 추출물(HFC)의 혈관신생 억제 효과를 확인하는 것이다. HFC가 세포 이동, 침입 및 관 형성을 포함하여 HUVEC 세포에 미치는 영향을 조사하였고, 또한 마우스 Matrigel 겔 플러그 분석을 통해 생체 내 혈관 신생 억제 효과도 조사하였다. 또한 HFC 처리 후 혈관신생에 중요한 인자인 VEGF, FGFR의 발현이 억제되고, Erk, Akt의 활성이 감소하는 것을 확인하였다. 이러한 결과는 해양갈조류 톳(H. fusiformis)의 클로르포름 추출물이 in vitro 및 in vivo 혈관 신생을 억제함을 보여준다.

• Tuberculosis and Respiratory Diseases
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• 제41권4호
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• pp.339-353
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• 1994

연구배경 : p53 유전자는 염색체 17p에 존재하는 종양억제유전자인데, 돌연변이가 있는 경우의 mutant protein은 그 반감기가 4~8시간으로 wild type protein의 반감기 6~20분에 비하여 현저한 증가를 보이게 된다. 결과적으로 돌연변이가 있는 경우 p53 단백질이 과축적되어 과발현이 유발되는데, 이러한 기전을 이용하여 p53 면역조직화학염색은 p53유전자의 돌연변이의 검색 방법으로 많이 이용되고 있다. 유방암에서는 p53 핵단백질의 과발현이 있는 경우에 없을 때보다 무병생존기간 및 전체 생존기간 모두에서 유의하게 나쁜 임상 경과를 취함이 보고되고 있지만 폐암에서는 p53 유전자의 돌연변이 유무, 또는 p53단백의 과발현 유무가 예후에 미치는 영향은 아직까지 확실하지 않다. 저자 등은 한국인의 폐암에서 p53 종양억제 유전자의 돌연변이 빈도를 확인하고 이들이 폐암 환자의 임상 경과에 미치는 영향을 분석하며, 면역조직화학염색 및 PCR-SSCP 분석의 감수성과 특이성을 염기서열분석과 비교하여 확인하고자 본 연구를 시행하였다. 방법 : 근치적 폐절제술 후 임상경과를 추적중인 원발성폐암환자 75예를 대상으로 하였으며, 면역조직화학검사 및 분자생물학적 연구를 위하여는 이들의 종양조직(paraffin block)을 이용하였다. p53 단백질의 면역조직화학검사를 위한 일차항체로는 DO7(Novocastra, U.K.)을 사용하였고, PCR-SSCP 및 염기서열분석 등을 위하여는 파라핀 포매조직에서 암조직을 선택적으로 박절하여 DNA를 추출하여 이용하였다. 결과: 1) 전체 75예 폐암환자의 면역조직화학염색 결과 27예(36%)에서 p53단백질의 과발현을 보였다(Table 2.3, Fig 1). 2) 전체 환자의 p53 핵단백질 과발현 여부에 따른 중앙전체생존기간은 p53 음성군과 양성군 모두 25개월, 무병생존기간은 두군 모두 13개월로 동일하였다(Fig. 2). 3) PCR-SSCP 분석에 의한 p53 유전자의 mobility shift는 시험한 58예중 16예(27.6%)에서 확인되었다(Fig 3). Mobility shift 유무에 따른 중앙전체생존기간은 shift가 있는 군과 없는 군에서 각각 27개월과 20개월, 무병생존기간은 각각 8개월, 10개월로 유의한 차이가 없었다. 4) 염기서열분석을 통한 p53 유전자의 돌연변이는 시험한 29예중 10예(34.5%)에서 확인되었다(Fig 4). 염기서열분석 결과 돌연변이 유무에 따른 중앙전체생존기간은 돌연변이가 있었던 군과 없었던 군에서 각각 27개월, 22개월이었으며, 무병생존기간은 각각 20개월과 10개월로 유의한 차이는 없었으나 돌연변이가 있는 경우 조기재발을 하는 경향을 보였다(Fig 5, 6). 5) 면역조직화학염색은 PCR-SSCP 결과를 기준으로 할 때 민감도 67.0%, 특이도 74.0%, 그리고 일치도는 62.5% 이었다. PCR-SSCP의 결과는 염기서열분석 결과를 기준으로 할 때 민감도 91.8%, 특이도 96.2%, 일치도는 95.3% 이었다. 결론 : p53 핵단백질의 과발현 정도, PCR-SSCP 및 염기서열분석상 돌연변이 유무는 비소세포폐암환자의 근치적수술후 예후의 예측지표로서는 그 효용성이 적었으나, 돌연변이가 있는 경우 조기재발을 하는 경향을 보였다. 또한 p53유전자의 돌연변이 검색법으로는 면역조직화학염색보다는 PCR-SSCP법이 우수하였다.

• 한국간호교육학회지
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• 제1권1호
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• pp.17-23
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• 1995

One of the biggest problems of Nursing Education in Korea is the division among nursing education programs of the last 3 and 4 years. To solve this problem, Nursing community must do variable trials to achieve the unity of a 4-year educational program. With this, we need to observe the phenomena and reality of the present 4-year nursing educational program that we have. The object of this study is to analyse and discuss that we have. The object of this study is to analyse and discuss the problems and future strategies of 4-year Nursing Baccalaureate program. 1. Problems as nursing department in Medical School. 1) Many 4-year nursing baccalaureate programs are operating under the medical school as nursing department. So the academic development in nursing department is unprogressive and is not approved as unique discipline. 2) The operating system between nursing and medical department are different even though they are in the same school. 3) Inequality between nursing and medical department : In many case, the nursing professor can not attend administraion committees to discuss the medical school's operation because of many differences between nursing and medical organization. 4) Weakness of the leadership and the student activities in nursing student : The nursing student involvement is usually passive because of the difference of curriculum, less number than medical students and the difference between 4-year and 6-year education program. 5) There is the obscurity of the relationship between department of nursing and other departments in whole university. 2. Problems in nursing itself 1) We need to reconstruct nursing discipline. We must change from the disease centered model to health centered model and life cycle centered model so that we can be distinguished from medicine. We also must change from hospital centered nursing to all population centered nursing, 2) The improvement of curriculum ; When the independent framework of nursing discipline become established, we need to improve the curriculum. 3) The education of clinical practice ; Most nursing school programs are divided into professors who are lecturing the theory and clinical teachers who are teaching the nursing technique in the clinic. So, what is needed in nursing discipline is that the professors have a dual position. In America, The professor is required to be a clinical specialist and to have his or her clinic so that the professor become a good role model, teach the clinical practice effectively, and give the student the practice field. 4) To extend fields of nursing : At first, the school nurse must become the school health educator, a real teacher. The nurse must establish and operate a childern's wellbeing center or nursery school, a disabled people's house or senile's wellbeing center, a mental health center, and a health promotion clinic for healthy people. 5) The name 'nursing department' need to be considered. When the focus is to be changed from the disease model to health improvement model, we take into consideration change 'nursing college', 'nursing department' and 'nursing profession' to 'health science college' or 'health wellbeing college'. 6) We must have highly qualified academic students. Each Nursing educational faculties must have the high qualified students through the development of nursing educational program and the increment of scholarship. The Korean Nurses Association and The Korean Clinical Nurses Association need to make an endeavor for the improvement of work condition and payment of clinical nurses of hospitals who consist of 70% of all nursing manpower. 3. Improvement Strategy 1) All nursing educational program must be changed 4-year program gradually. 2) Nursing department need to try to become nursing college. 3) We need to study many researches for improvement of the problem in nursing discipline and nursing education. We need more interdisciplinary researches, and we need to be granted for that research. 4) We need to have many seminars and workshops thoughout the whole country to expand a sense of nursing education. 5) Drawing up a policies plan for the nursing educational improvement : The Korean Nurses Association, The Korean Academic Nursing Association, Korea Nursing College and department President's Committee, and Korea Academic Society of Nursing Education must try for the development of nursing educational improvement and ask for government frame the policy to develop nursing education.