• 경영과학
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• 제22권1호
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• pp.15-26
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• 2005

The bootstrap is a method of computational inference that simulates the creation of new data by resampling from a single data set. We propose a new job for the bootstrap: generating inputs from one historical trace using Threshold Bootstrap. In this regard, the most important quality of bootstrap samples is that they be functionally indistinguishable from independent samples of the same stochastic process. We describe a quantitative measure of difference between two time series, and demonstrate the sensitivity of this measure for discriminating between two data generating processes. Utilizing this distance measure for the task of generating inputs, we show a way of tuning the bootstrap using a single observed trace. This application of the threshold bootstrap will be a powerful tool for Monte Carlo simulation. Monte Carlo simulation analysis relies on built-in input generators. These generators make unrealistic assumptions about independence and marginal distributions. The alternative source of inputs, historical trace data, though realistic by definition, provides only a single input stream for simulation. One benefit of our method would be expanding the number of inputs achieving reality by driving system models with actual historical input series. Another benefit might be the automatic generation of lifelike scenarios for the field of finance.

• The Plant Pathology Journal
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• 제20권4호
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• pp.247-251
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• 2004

We developed a polyclonal antibody (PAh) based- ELISA system to accurately and rapidly monitor inocula on plant surface before onset of anthracnose. Titer of mouse antisera against conidia of Colletotrichum gloeosporioides was determined by using indirect ELISA. It was high enough to be detectable up to ${\times}$ 12,800 dilutions. Absorbance readings exceeded (1.5even at a 10$^{-5}$ dilution. Sensitivity of PAb was precise enough to detect spore concentration as low as 50 conidia/well by indirect ELISA. PAb1 and PAb2 proved to be very sensitive and highly specific to the target pathogen, C. gloeosporioides, apparently discriminating other unrelated pathogens, or epiphytes. Absorbance values for original isolate exceeded 1.0, but no reaction was detected with other isolates, except three other anthracnose fungi: C. gloeosporioides (pepper strain), Glomerella cingulata (apple strain) and C. lagenarium. Our data suggest that PAb1 and PAb2 bind with the protein epitope that partially contains residues of amino acid, arginine, and Iysine. This kit fulfills the require-ments for detecting inoculums before infection and during onset of anthracnose on sweet persimmon.

• Journal of Microbiology and Biotechnology
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• 제27권1호
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• pp.72-76
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• 2017

Detection of exon 19 deletion mutation in the epidermal growth factor receptor (EGFR) gene, which results in increased and sustained phosphorylation of EGFR, is important for diagnosis and treatment guidelines in non-small-cell lung cancer. Here, we have developed a simple and convenient detection system using the interaction between G-quadruplex and fluorophore thioflavin T (ThT) for discriminating EGFR exon 19 deletion mutant DNA from wild type without a label and quencher. In the presence of exon 19 deletion mutant DNA, the probe DNAs annealed to the target sequences were transformed into G-quadruplex structure. Subsequent intercalation of ThT into the G-quadruplex resulted in a light-up fluorescence signal, which reflects the amount of mutant DNA. Due to stark differences in fluorescence intensity between mutant and wild-type DNA, we suggest that the induced G-quadruplex structure in the probe DNA can report the presence of cancer-causing deletion mutant DNAs with high sensitivity.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2000년도 춘계학술대회논문집
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• pp.670-678
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• 2000

Loose parts monitoring system(LPMS) is one of the important monitoring systems for the safe and efficient operation of the nuclear reactor, since it is LPMS that can early detect loose parts which may cause a significant damage in facilities or components of the plant. Nuclear power plants in Korea have recently experienced several loose part alarms due to the metallic impact and it is expected that the frequency of the loose part will be increased along the aging of the plants. In this paper, the status of loose parts monitoring technologies and facilities in Korean nuclear power plants is presented for the establishment of LPMS installation plan in some nuclear reactors which are not yet equipped with LPMS. Sensor specification, location and mounting method for loose parts monitoring were reviewed. As a result, the location and the mounting method of the properly chosen sensor was recommended. Data acquisition algorithms and discriminating rules of loose part impact signals were also reviewed. Actual alarm cases occurred by true impact signal and false impact signal were stated here.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2002년도 하계학술대회 논문집 C
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• pp.1569-1571
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• 2002

The determination of DNA hybridization can apply the molecular biology research, clinic diagnostics, bioengineering, environment monitoring, food science and other application area. So, The determination of hybridization is very important for the improvement of DNA detection system. In this study, we report the characterization of the DNA hybridization by the electricalchemical methods. A new electrochemical biosensor is described for voltammetric detection of gene sequence related to probe oligonucleotide of bacterium Escherichia coli O157:H7. The biosensor involves the immobilization of a 18-mer probe oligonucleotide, which is complemetary to a specific gene sequence related to Escherichia coli O157:H7 on a gold electrode through specific adsorption. The probe oligonucleotide was used to determine the amount of target oligonucleotide in solution using mitoxantrone(MTX) as the electrochemical indicators. The cathodic peak currents $(I_{peak})$ of MTX were linearly related to the concentration of the target oligonucleotide sequence in the range $1[{\mu}M]{\sim}0.1[nM]$. The detection limit of this approach was 0.01[nM]. In addition, these indicators were capable of selectivity discriminating against various mismatching condition.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.755-764
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• 1996

A multispectral imaging system with selected wavelength optical filter was demonstrated feasible for food safety inspection. Intensified multispectral images of carcasses were obtained with visible/near-infrared optical filters(542-847 nm wavelengths) and analyzed. The analysis of textural features based on co-occurrence matrices was conducted to determine the feasibility of a multispectral image analyses for discriminating unwholesome poultry carcasses from wholesome carcasses. The mean angular second moment of the wholesome carcasses scanned at 542 nm wavelength was lower than that of septicemic (P$\leq$0.0005) and cadaver(P$\leq$0.0005) carcasses. On the other hand, for the carcasses scanned at 700nm wavelength , the feature values of septicemic and cadaver carcasses were significantly (P$\leq$0.0005) different from wholesome carcasses. The discriminant functions for classifying poultry carcasses into three classes (wholesome, septicemic , cadaver) were developed using linear and quadr tic covariance matrix analysis method. The accuracy of the quadratic discriminant models, expressed in rates of correct classification, were over 90% for the classification of wholesome, septicemic, and cadaver carcasses when textural features from the spectral images scanned at the wavelength of 542 and 700nm were utilized.

• 한국광학회지
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• 제9권2호
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• pp.96-99
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• 1998

본 연구에서는 새로운 광피드백 장기 안정화법을 제안하고 실험적으로 그 가능성을 입증하였다. 제안한 방법은 광로장을 제어하는 방법으로써 지금까지와는 다른 고감도의 광피드백 위상 변별 기법을 도입하였다. 이는 외부 반사체로 사용한 공초점 패브리-페로 공진기의 반사모드의 편광 분광법에 의하여 위상 변별 신호를 얻어내는 것이다. 실험 결과 약 20시간 정도의 안정된 제어 상태를 유지하였다. 이 실험에서 사용한 광학계가 각각의 분리된 광학 요소를 광학 테이블에 배열한 불안정한 상태였으므로 광학계의 크기를 줄이고 각 광학적 요소를 하나의 판 위에 고정시킴으로서 일체화시킨다면 안정된 제어의 유지시간을 대폭 늘일 수 있을 것이다.

• 한국추진공학회지
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• 제10권2호
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• pp.102-109
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• 2006

본 논문에서 항공기용 터보 축 엔진의 결함 진단 알고리즘을 개발하기 위해 Support Vector Machine(SVM)과 인공신경망(ANN)을 이용하였다. 신경망을 이용한 시스템은 비선형성이 과도한 데이터를 학습할 때 지역 최소점(Local Minima)에 빠져 분류 정확률이 낮아질 수 있다. 이러한 위험성을 보안하기 위해 SVM에 의한 ANN의 분할 학습 알고리즘(SLA)을 제안하였다. 이것은 SVM을 이용하여 결함 위치를 판별 한 후 신경망이 선택적으로 학습을 하는 방법으로 학습 데이터의 비선형성을 줄여 분류 정확률을 높이기 때문에 신경망을 단독으로 사용할 때보다 개선된 성능을 보여주었다.

• Bulletin of the Korean Chemical Society
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• 제14권1호
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• pp.91-95
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• 1993

In this work we have studied the spin-lattice relaxation of $^{19}$F spins in benzotrifluoride in our quest for a reliable method of discriminating the contribution due to dipolar relaxation mechanism from that due to spin-rotational mechanism for nuclear spins located on methyl or substituted methyl group in organic molecules. Over the temperature range of 248-268 K the decay of normalized longitudinal magnetization was found to be well described by a two parameter equation of the form R(t) = exp(-st){$\frac{5}{6}$exp(-s$_1$)+$\frac{1}{6}$} which was derived under the assumption that interactions in the A3 spin system are modulated randomly and predominantly by internal rotational motions of -CF_3$ top, and it was shown that the separation of contribution due to dipolar interactions from that due to spin-rotation interaction could be successfully achieved by least-square fitting of observed data to this equation. The results indicate that the spin-rotational contribution is overwhelmingly larger than that of dipolar origin over the given temperature range and becomes more deminating at higher temperature.

• Clinical and Experimental Pediatrics
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• 제64권3호
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• pp.103-110
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• 2021

Inflammatory or immune-mediated demyelinating central nervous system (CNS) syndromes include a broad spectrum of clinical phenotype and different overlapping diseases. Antibodies against myelin oligodendrocyte glycoprotein (MOG-Ab) have been found in some cases of these demyelinating diseases, particularly in children. MOG-Ab is associated with a wider clinical phenotype not limited to neuromyelitis optica spectrum disorder, with most patients presenting with optic neuritis, acute disseminated encephalomyelitis (ADEM) or ADEM-like encephalitis with brain demyelinating lesions, and/or myelitis. Using specific cell-based assays, MOG-Ab is becoming a potential biomarker of inflammatory demyelinating disorders of the CNS. A humoral immune reaction against MOG was recently found in monophasic diseases and recurrent/multiphasic clinical progression, particularly in pediatric patients. This review summarizes the data regarding MOG-Ab as an impending biological marker for discriminating between these diverse demyelinating CNS diseases and discusses recent developments, clinical applications, and findings regarding the immunopathogenesis of MOG-Ab-associated disorders.