• Title/Summary/Keyword: Discriminated analysis

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A Study on the Damage Estimation of Uni-directionally Oriented Carbon Fiber Reinforced Plastics using Acoustic Emission (음향방출을 이용한 일방향 탄소섬유강화 플라스틱의 손상평가에 관한 연구)

  • Rhee Zhang-Kyu;Park Sung-Oan;Kim Bong-Gag;Woo Chang-Ki
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.14 no.1
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    • pp.30-36
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    • 2005
  • This study is to investigate a damage estimation of single edge notched tensile specimens as a function of acoustic emission(AE) according to the uni-directionally oriented carbon fiber/epoxy composites, CFRP In fiber reinforced composite materials, AE signals due to several types of failure mechanisms are typically observed. These are due to fiber breakage, fiber pull-out matrix cracking, delamination, and splitting or fiber bundle breaking. And these are usually discriminated on the basis of amplitude distribution, event counts, and energy related parameters. In this case, AE signals were analyzed and classified 3 regions by AE event counts, energy and amplitude for corresponding applied load. Bath-tub curve shows 3 distinct periods during the lifetime of a single-edge-notch(SEN) specimen. The characterization of AE generated from CFRP during SEN tensile test is becoming an useful tool f3r the prediction of damage failure or/and failure mode analysis.

Discrimination of artificial explosions by using seismo-acoustic data in 2004 and installation of BRDAR (지진-음파 자료를 이용한 2004년도 인공발파 식별과 백령도 지진-음파 관측망 설치)

  • Che, Il-Young;Jeon, Jeong-Soo;Shin, In-Cheol
    • Proceedings of the Earthquake Engineering Society of Korea Conference
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    • 2005.03a
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    • pp.68-73
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    • 2005
  • In succession of the previous works, seismo-acoustic analysis was conducted to collect ground truth events and to discriminate surface explosions from natural earthquakes in the Korean Peninsula for 2004. In this period, total 510 seismo-acoustic events corresponding to 10.8 percent of total seismic events occurred in and near the Korean Peninsula were analyzed and discriminated as artificial surface explosions. Events distribution of the seismo-acoustic events in 2004 is similar to the previous results of 1999-2003. And newly determined seismo-acoustic events were added to the surface explosions database. To extend infrasound detection capability, Korea Institute of Geoscience and Mineral Resources (KIGAM) and Southern Methodist University (SMU) installed new seismo-acoustic array (BRDAR) in Baekryoung Island last November, 2004. The array configuration and design is nearly same to previous seismo-acoustic arrays CHNAR, KSGAR, a triangular 1 km aperture. BRDAR consists of 5 short period vertical seismometers (GS-13) in seismic vaults and 13 microbarometers (Chaparral Model 2). Preliminary analysis using data collected from BRDAR shows an extension of infrasound detection capability to western part of the Korean Peninsula. Also, multiple observations of infrasound at BRDAR and other arrays gave an opportunity to localize sound source regions.

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The Detection of Irradiated Agricultural Commodities by Origins with Photostimulated Luminescence (PSL) Analysis (Photostimulated Luminescence (PSL) 분석에 의한 원산지별 농산물의 방사선 조사여부 검지)

  • 정재영;이은영;권중호
    • Food Science and Preservation
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    • v.8 no.3
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    • pp.291-295
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    • 2001
  • Photostimulated luminescence (PSL) analysis was applied to determine whether some agricultural commodities of Korean and Chinese origins have been irradiated or not. Cereals (brown rice and buckwheat) and legumes (soybean, peanut, fed bean and mung bean) were exposed to gamma irradiation at 0.5 ∼4 kGy The Korean garlic was irradiated at 0.05 ∼0.5 kGy. Cereals and mung bean were tool applicable to the PSL measurement, showing the intermediate values between the lower (700 photon counts, negative) and the upper threshold values (5,000, positive), however PSL values determined for soybean, peanut, red bean and garlic were suitable for being discriminated between nonirradiated and irradiated samples. There was no significant difference in PSL properties accgrding to the sample origins.

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Species Identification of Five Penaeid Shrimps Using PCR-RFLP and SSCP Analyses of 16S Ribosomal DNA

  • Khamnamtong, Bavornlak;Klinbunga, Sirawut;Menasveta, Piamsak
    • BMB Reports
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    • v.38 no.4
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    • pp.491-499
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    • 2005
  • DNA-based molecular markers for differentiation of five penaeid shrimps (Penaeus monodon, P. semisulcatus, Feneropenaeus merguiensis, Litopenaeus vannamei and Marsupenaeus japonicus) were developed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-stranded conformation polymorphism (SSCP) of 16S ribosomal (r) DNA. Differentiation of P. monodon, P. semisulcatus and L. vannamei can be unambiguously carried out by PCR-RFLP of 16S $rDNA_{560}$ whereas P. semisulcatus and M. japonicus shared a BABB mitotype. These shrimps were successfully discriminated by SSCP analysis of 16S $rDNA_{560}$. Nevertheless, the amplification success for L. vannamei and F. merguiensis was not consistent when tested against larger sample sizes. As a result, 16S $rDNA_{560}$ of an individual representing the most common mitotype of each species was cloned and sequenced. The new primer pair was designed and tested against the large sample sizes (312 bp product, N = 185). The amplification success was consistent across all species. PCR-RFLP of 16S $rDNA_{312}$ was as effective as that of 16S $rDNA_{560}$. Differentiation of all shrimp species were successfully carried out by SSCP analysis.

Genetic Distances between Two Echiuran Populations Discriminated by PCR

  • Yoon, Jong-Man
    • Development and Reproduction
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    • v.23 no.4
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    • pp.377-384
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    • 2019
  • Genomic DNA extracted from representatives of two populations, Gunsan and Chinese, of Urechis spp. was amplified using PCR with several primers. The band-sharing (BS) value between individuals no. 05 from the Gunsan population and no. 22 from the Chinese population was 0.206, which was the lowest recognized value. Oligonucleotides primer OPC-04 revealed 44 unique loci, which distinguished the Chinese population. Primer OPB-17 allowed the discovery of 22 loci shared by the two populations, which were present in all samples. Based on the average BS results, individuals from the Gunsan population demonstrated lower BS values (0.661±0.012) than did those from the Chinese population (0.788±0.014; p<0.05). The shortest genetic distance (GD) displaying a noteworthy molecular difference was between individuals CHINESE no. 12 and no. 13 (GD=0.027). Individual no. 06 from the Gunsan population was most distantly related to CHINESE no. 22 (GD=0.703). A group tree of the two populations was constructed by UPGMA Euclidean GD analysis based on a total of 543 fragments generated using six primers. The explicit markers recognized in this study will be used for genetic analysis, as well as to evaluate the species security and proliferation of echiuran individuals in intertidal regions of the Korean Peninsula.

The Analysis of Factors on the Service-Linkage of Long-term Care Workers for the Elderly (일부 노인 장기요양보호기관 종사자간의 서비스연계 조사)

  • You, Jae-Eung;Kim, Kyoung;Cha, Yong-Jun
    • The Journal of Korean Physical Therapy
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    • v.24 no.1
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    • pp.35-40
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    • 2012
  • Purpose: This study was to analyze the factors that affect the service relationship of long term care workers for the elderly and to provide basic resource for the successful connection of long term care services. Methods: 259 subjects who were engaged in long term care units completed a self-administered questionnaire that measured the extent of service linkage among one another. The Cronbach's ${\alpha}$ score determined the internal consistency of the acquired data and the discriminated validity was estimated by Pearson's correlation coefficient. Multiple regression analysis was conducted to investigate the influence of the known factors on the service linkage. Results: Acceptance and participation negatively influenced on the service linkage. Reliance, comprehension, recognition on service, and frequent contact with others positively activated the service linkage of long term care workers. Conclusion: The establishments of systemic training courses providing education that emphasizes reliability and recognizes other services, including work environment to contact easily are needed to improve the service-linkage of long-term care workers for the elderly.

Flavor Pattern Analysis of Imported Wines Using Electronic Nose System (포도주의 전자코(Electronic Nose)를 이용한 향기 패턴 분석)

  • Kim, Ji-Young;Jang, Ji-Sun;Lee, Ju-Woon;Lee, Ki-Teak
    • Journal of the East Asian Society of Dietary Life
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    • v.18 no.1
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    • pp.14-21
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    • 2008
  • Flavor is one of the most important factors for determining wine characteristics and quality. Flavor pattern of wines(brewed from America, France, Italy, Chile, and Australia) was analyzed by the electronic nose that is equipped with 12 metal oxide sensors. In the results, the flavor pattern of wines was discriminated according to their origins by the principal component analysis(PCA). Each proportion of the first principal component score in the PCA plot was 94.79%(America), 73.62%(France), 99.06%(Italy), 96.74%(Chile), and 96.53%(Australia), respectively. Consequently, the imported wines could be practically differentiated into one from the other origins by volatile properties, suggesting that electronic nose could be successfully used for easy screening and quality evaluation of wines.

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Liquid Chromatography-Mass Spectrometry-Based Chemotaxonomic Classification of Aspergillus spp. and Evaluation of the Biological Activity of Its Unique Metabolite, Neosartorin

  • Lee, Mee Youn;Park, Hye Min;Son, Gun Hee;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.23 no.7
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    • pp.932-941
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    • 2013
  • This work aimed to classify Aspergillus (8 species, 28 strains) by using a secondary metabolite profile-based chemotaxonomic classification technique. Secondary metabolites were analyzed by liquid chromatography ion-trap mass spectrometry (LC-IT-MS) and multivariate statistical analysis. Most strains were generally well separated from each section. A. lentulus was discriminated from the other seven species (A. fumigatus, A. fennelliae, A. niger, A. kawachii, A. flavus, A. oryzae, and A. sojae) with partial least-squares discriminate analysis (PLS-DA) with five discriminate metabolites, including 4,6-dihydroxymellein, fumigatin, 5,8-dihydroxy-9-octadecenoic acid, cyclopiazonic acid, and neosartorin. Among them, neosartorin was identified as an A. lentulus-specific compound that showed anticancer activity, as well as antibacterial effects on Staphylococcus epidermidis. This study showed that metabolite-based chemotaxonomic classification is an effective tool for the classification of Aspergillus spp. with species-specific activity.

Interpretation of the Paleoclimate Environment using Large Plant Fossil of Peatland in Pyeongtaek, Central Korea

  • Lee, Jin-Hee;Cho, Kyu-Tae
    • Korean Journal of Environment and Ecology
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    • v.29 no.1
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    • pp.81-87
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    • 2015
  • This study was carried out in the peatland for estimating the paleoenvironment based on the analysis from its fossils plant and radiocarbon at the Hwayang-ri, Hyeondeok-myeon, Pyeongtaek-city, South Korea. Variety of fossil was collected from the peatland which could discriminated with naked eye and sorted into seeds, leaves, stems from four-stage of standard sieve. During analysis for the large plant fossil within the peatland, the dead plants were largely divided into 3 fossil zones according to its peatland. In the fossil zone III (the oldest layer; 6,970~6,070 yr $C^{14}$ B.P.), the observation of leaves of hydrophyte such as Trapa sp. and stems of Alnus japonica which appeared in wetland means that the environment was influenced by the climate changes such as rainfall increasing and sea level rise. In the fossil zone II (the middle layer; 6,070~5,800 yr C14 B.P.), the occurrence of Gramineae, Cyperaceae and Fagaceae indicate that the environmental condition might be more or less dry by decreasing rainfall and drop in sea level. The fossil zone I (the recent layer; 5,800~4,540 yr $C^{14}$ B.P.) where lots of herbaceous plants, increasing of Fagaceae, decreasing of A. japonica. and distribution of Pinaceae were showed, was inferred to be repeated both dry and wet environment due to human disturbance.

Metabolomics-Based Chemotaxonomic Classification of Streptomyces spp. and Its Correlation with Antibacterial Activity

  • Lee, Mee Youn;Kim, Hyang Yeon;Lee, Sarah;Kim, Jeong-Gu;Suh, Joo-Won;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.25 no.8
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    • pp.1265-1274
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    • 2015
  • Secondary metabolite-based chemotaxonomic classification of Streptomyces (8 species, 14 strains) was performed using ultraperformance liquid chromatography-quadrupole-time-offlight-mass spectrometry with multivariate statistical analysis. Most strains were generally well separated by grouping under each species. In particular, S. rimosus was discriminated from the remaining sevens pecies (S. coelicolor, S. griseus, S. indigoferus, S. peucetius, S. rubrolavendulae, S. scabiei, and S. virginiae) in partial least squares discriminant analysis, and oxytetracycline and rimocidin were identified as S. rimosus-specific metabolites. S. rimosus also showed high antibacterial activity against Xanthomonas oryzae pv. oryzae, the pathogen responsible for rice bacterial blight. This study demonstrated that metabolite-based chemotaxonomic classification is an effective tool for distinguishing Streptomyces spp. and for determining their species-specific metabolites.