• Applied Microscopy
• /
• v.51
• /
• pp.2.1-2.7
• /
• 2021

Synaptic vesicles, which are endogenous to neurotransmitters, are involved in exocytosis by active potentials and release neurotransmitters. Synaptic vesicles used in neurotransmitter release are reused via endocytosis to maintain a pool of synaptic vesicles. Synaptic vesicles show different types of exo- and endocytosis depending on animal species, type of nerve cell, and electrical activity. To accurately understand the dynamics of synaptic vesicles, direct observation of synaptic vesicles is required; however, it was difficult to observe synaptic vesicles of size 40-50 nm in living neurons. The exo-and endocytosis of synaptic vesicles was confirmed by labeling the vesicles with a fluorescent agent and measuring the changes in fluorescence intensity. To date, various methods of labeling synaptic vesicles have been proposed, and each method has its own characteristics, strength, and drawbacks. In this study, we introduce methods that can measure presynaptic activity and describe the characteristics of each technique.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.21 no.12
• /
• pp.772-781
• /
• 2020

A direct shear member resists external forces through the shear transfer of reinforcing bars placed at the concrete interface. The current concrete structural design code uses empirical formulas based on the shear friction analogy, which is applied to the horizontal shear of concrete composite beams. However, in the case of a member with a large amount of reinforcing bars, the shear strength obtained through the empirical formula is lower than the measured value. In this paper, the limit state of newly constructed composite beams on an existing concrete girder is defined using stress field theory, and material constitutive laws are applied to gain horizontal shear strength while considering the tension-stiffening and softening effects of concrete struts. A simplified method of calculating the shear strength is proposed, which was validated by comparing it with the related design code provisions. As a result, it was confirmed that the method generally shows a similar tendency to the experimental results when the shear reinforcing bar yields, unlike the regulations of the design code, where differences in the predicted value of shear strength occur according to the shear reinforcement ratio.

• Journal of Korean Association for Spatial Structures
• /
• v.12 no.3
• /
• pp.63-70
• /
• 2012

In this study, the displacement-based design concept, the performance by the existing reinforced concerte column and steel reinforced concrete composite column for SRC purchased the maximum design ground acceleration improvement compared to the performance design. SRC have several advantages such as strength enhancement and high ductility. H-beam or steel tubes were used for embedded elements of the SRC composite columns. SRC cross-section for the P-M diagram and analysis on the nominal bending monent SRC designed for composite columns for disparity estimation is presented to the displacement-based seismic design. Performance improvement of the performance-based design performance targets for the design seismic displacement and design criteria for the direct displacement-based design methods and to improve the seismic performance due to the displacement coefficient method is proposed to design. SRC compared with the RC column designed to improve the performance and displacement ductility ratio displacement results in the performance design results showed significantly improved performance.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.39 no.5
• /
• pp.425-433
• /
• 2015

In this paper, we have numerically investigated two-dimensional dielectrophoretic (DEP) motions of a single particle suspended freely in a viscous fluid, interacting with a nearby nonconducting planar wall, under an externally applied uniform direct-current electric field. Particularly, we solve the Maxwell equation with a large sharp jump in the electric conductivity at the particle-fluid interface and then integrate the Maxwell stress tensor to compute the DEP force on the particle. Results show that, under an electric field parallel to the wall, one particle is always repelled to move far away from the wall and the motion depends strongly on the particle-wall spacing and the particle conductivity. The motion strength vanishes when the particle is as conductive as the fluid and increases as the conductivity deviates further from that of the fluid.

• Journal of Korean Society of Steel Construction
• /
• v.20 no.5
• /
• pp.633-644
• /
• 2008

The structural framework design uses high-strength bolts and welding in column splices. However, for the column under high compression, the number of the required high-strength bolts can be excessive and the increase of welding results in difficulty of quality inspection, the transformation of the structural steels, and the increase of erection time. According to the AISC criteria, when columns have bearing plates, or they are finished to bear at splices, there shall be sufficient connections to hold all parts securely in place. The Korean standard sets the maximum 25% of the load as criteria. Using direct contact makes it possible to transfer all compressive force through it. The objective of this study is to examine the generally applied stress path mechanism of welded or bolted columns and to verify the bending moment and compression transfer mechanism of the column splice according to metal touch precision. For this study,22 specimens of various geometric shapes were constructed according to the change in the variables for each column splice type, which includes the splice method, gap width, gap axis, presence or absence of splice material, and connector type. The results show that the application of each splice can be improved through the examination of the stress path mechanism upon metal contact. Moreover, the revision of the relative local code on direct contact needs to be reviewed properly for the economics and efficiency of the splices.

• Journal of Korean Tunnelling and Underground Space Association
• /
• v.17 no.2
• /
• pp.127-140
• /
• 2015

A fault is one of the critical factors that may lead to a possible ground collapse occurring in construction site. A fault core, however, possibly acting as a failure plane in whole fault zone, is composed of fractured rock and gouge nonuniformly distributed and thus can be characterized by its wide range of shear strength which is generally acquired by experimental method for stability analysis. In this study, we performed direct shear test and grain size distribution analysis for 62 fault core samples cropped from 12 different spots located in the vicinity of Kyongju and Ulsan, Korea. As a result, the range of shear strength representing the characteristics of fault cores in the study regions is determined with regard to vertical stress using a regression analysis for experiment data. The weight ratio of gravels in the samples is proportional to the shear strength and that of silt and clay is in inverse proportion to the shear strength. For most samples, the coefficient of determination is over 0.7 despite of inhomogeneity of them and consequently we determined the lower limit and upper limit of the shear strength with regard to the weight ratio by setting the confidence interval of 95%.

• Magazine of the Korea Concrete Institute
• /
• v.11 no.1
• /
• pp.231-242
• /
• 1999

This paper presents a numerical study on the flat plates in deep basements, subjected to out-of-plane floor load and in-plane compressive load due to soil and hydraulic lateral pressure. For nonlinear finite element analysis, a computer program addressing material and geometric nonlinearities is developed. The validity of the numerical model is established by comparison with existing experiments performed on plates simply supported on four edges. The flat plates to be studied are designed according to the Direct Design Method in Korean Building Code for Structural Concrete. Through numerical study on the effects of different load combinations and loading sequence, the load condition that governs the strength of the flat plates is determined. For the plates under the governing load condition, parametric studies are performed to investigate variations of the strength with reinforcement ratio, aspect ratio, concrete strength, and slenderness ratio. Based on the numerical results, the floor load magnification factor is proposed.

• Journal of the Korean Geotechnical Society
• /
• v.23 no.5
• /
• pp.89-100
• /
• 2007

In this study, various field and laboratory tests were performed to investigate the characteristics of shear strength and bearing behavior to be considered in the estimation of stability and trafficability in early stage of stabilization process in marine clay dredged deposit. Site characterization was carried out to grasp the basic properties of the deposit. Field vane test, unconfined compression test and direct shear test were conducted to evaluate the shear strength distribution for varied depths, and the characteristics of shear strength and stress-strain behavior of the crust layer. Plate load tests were also performed to estimate the bearing capacity and to assess load-settlement behavior and failure pattern of the deposit. The bearing capacity was also estimated using previously proposed methods for double-layered clay deposit. The estimated bearing capacity was compared with the results of the plate load tests and then, the applicability of the estimation method was discussed.

• Journal of the Korea Concrete Institute
• /
• v.13 no.1
• /
• pp.46-53
• /
• 2001

Two full scale precast pretensioned dapped ended rectangular beams designed by PCI design handbook for a major domestic live load of market and parking building - 500kgf/㎡ and 1,200kgf/㎡ were investigated experimentally. The bottom length of beams was 60cm which was same to the length of rectangular column in the base of five-story market or parking buildings. The height of dap was web hight plus half of the flange height within the allowable limit of PCI method. Shear tests were performed on four beam ends. Followings were obtained from the experimental study. All of the specimens were fully complied with the PCI design handbook. Two of four specimens which were designed for live load of 1,200kgf/㎡ showed crackings at the re-entrant corner of dap before the full service loadings, and failed by direct shear at the load level much less than their calculated nominal strength. The specimens designed for live load of 1,200kgf/㎡ failed at 772 tonf and 78.36tonf by direct shear crackings. This strength was less than PCI limit of 81.9 tonf and higher than ACI limit of 65.62tonf. Thus, the limit suggested by ACI seems more reasonable in regard of safety in view of this test results. According to load-strain curves, the strain of hanger reinforcement reached almost yield strain. It is recommended to use more inclined hanger reinforcement of improve the strength and serviceability.

• The Journal of Engineering Geology
• /
• v.21 no.1
• /
• pp.57-64
• /
• 2011

Three-dimensional slope stability analysis was applied to a failed dual-lithology slope containing both granite and an andesitic dyke, taking account of the differences in shear strength of the different lithologies. A direct shear test of the soil-rock boundary was performed to examine the shear strength of two different types of failure surfaces within different lithologies, and a laboratory test was performed on an upper, weathered soil layer. The test results indicate that shear strength was lower at the soil-rock boundary than within the weathered soil layer. A representative geological section was subjected to two-dimensional slope stability analysis using a limit equilibrium method to assess whether the distribution of lithologies upon the slope influences the results of stability analysis. The results were then compared with those of three-dimensional slope stability analysis, for which input parameters can be varied according to the distribution of lithologies upon the slope. The three-dimensional analysis yielded safety factors of 1.26 under dry conditions and 0.55 under wet conditions, whereas the two-dimensional analysis yielded unstable safety factors of 0.92 and 0.32, respectively. These findings show that the results of stability analysis are affected by the distribution of different lithologies upon the slope. Given that the studied slope collapsed immediately after rainfall, it is likely that the results of the three-dimensional analysis are more reliable.