• 제목/요약/키워드: Direct gradient analysis

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PCR-DGGE를 이용한 해양미생물의 다양성 조사 (Diversity of Marine Microbes by PCR-DGGE)

  • 김영진;조효진;유선녕;김광연;김형락;안순철
    • 한국수산과학회지
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    • 제40권6호
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    • pp.356-361
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    • 2007
  • Recently, the development of various culture-independent identification techniques for environmental microbes has greatly enhanced our knowledge of microbial diversity. In particular, denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments, generated using the polymerase chain reaction (PCR) is frequently used to examine the diversity of environmental bacterial populations. This method consists of direct extraction of the environmental DNA, amplification of the 200-600 bp 16S rDNA fragments with universal primers, and separation of the fragments according to their melting point on a denaturing gradient gel. In this study, we investigated the seaside microbial community in coastal areas of Busan, Korea, using culture-independent techniques. First, marine genomic DNA was extracted from seawater samples collected at Songjeong, Gwangahn, and Songdo Beaches. Then, PCR was used to amplify the bacterial 16S rDNA using universal primers, and DGGE was used to separate the amplified 500 bp 16S rDNA fragments. Finally, the tested 16S rDNA genes were further analyzed by sequencing. Based on these experiments, we found that DGGE analysis clearly showed variation among the regional groups. It can be used to monitor rapid changes in the bacterial diversity of various environments. In addition, the sequence analysis indicated the existence of many unculturable bacteria, in addition to Arcobacter, Pseudoaltermonas, and Vibrio species.

경사기능성 세라믹/금속 복합재료의 열응력 해석(1)-플라즈마 용사재- (Thermal Stress Analysis of Functuonally Graded Ceramic/Metal Composites(I)-Plasma Spraying Material-)

  • 송준희;임재규;정세희
    • 대한기계학회논문집A
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    • 제21권3호
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    • pp.439-446
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    • 1997
  • A traditional notion of composites has been composed as a uniform dispersoid, but now it is proposed without regard to such rule with process development. Functionally Graded Material(FGM) consists of a new material design that is to make intentionally irregular dispersion state. In this study, thermal stress analysis of plasma spraying PSZ/NiCrAlY gradient material was conducted theoretically using a finite-element program. A formations of the model are direct bonding material(NFGM) and FGM with PSZ and NiCrAlY component element. The temperature conditions were $700^{\circ}C$ to 1100.deg. C assuming a cooling-down precess up to room temperature. Fracture damage mechanism was analyzed by the parameters of residual stress.

박판 구조물의 방사 소음에 대한 크기설계 민감도 해석 및 최적 설계 (Sizing Design Sensitivity Analysis and Optimization of Radiated Noise from a Thin-body)

  • 이제원;왕세명
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2003년도 춘계학술대회논문집
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    • pp.1038-1043
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    • 2003
  • There are many industrial applications including thin-body structures such as fins. For the numerical modeling of radiation of sound from thin bodies, the conventional boundary element method (BEM) using the Helmholtz integral equation fails to yield a reliable solution. Therefore, many researchers have tried to solve the thin-body acoustic problems. In the area of the design sensitivity analysis (DSA) and optimization methods, however, there has been just a few study reported. Especially fur the thin-body acoustics, however, no further study in the DSA and optimization fields has been reported. In this research, the normal derivative integral equation is adopted as an analysis formulation in the thin-body acoustics, and then used for the sizing DSA and optimization. Since the gradient-based method is used for the optimization, it is important to have accurate gradients (design sensitivities) of the objective function and constraints with respect to the design variables. The DSA formulations are derived through chain-ruled derivatives using the finite element method (FEM) and BEM by using the direct differentiation and continuum variation concepts. The proposed approaches are implemented and validated using a numerical example.

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역직구 상품 추천 및 판매가 추정을 위한 머신러닝 모델 (Machine Learning Model for Recommending Products and Estimating Sales Prices of Reverse Direct Purchase)

  • 김규익;볘르드바에브 예르갈리;김수형;김진석
    • 산업경영시스템학회지
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    • 제46권2호
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    • pp.176-182
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    • 2023
  • With about 80% of the global economy expected to shift to the global market by 2030, exports of reverse direct purchase products, in which foreign consumers purchase products from online shopping malls in Korea, are growing 55% annually. As of 2021, sales of reverse direct purchases in South Korea increased 50.6% from the previous year, surpassing 40 million. In order for domestic SMEs(Small and medium sized enterprises) to enter overseas markets, it is important to come up with export strategies based on various market analysis information, but for domestic small and medium-sized sellers, entry barriers are high, such as lack of information on overseas markets and difficulty in selecting local preferred products and determining competitive sales prices. This study develops an AI-based product recommendation and sales price estimation model to collect and analyze global shopping malls and product trends to provide marketing information that presents promising and appropriate product sales prices to small and medium-sized sellers who have difficulty collecting global market information. The product recommendation model is based on the LTR (Learning To Rank) methodology. As a result of comparing performance with nDCG, the Pair-wise-based XGBoost-LambdaMART Model was measured to be excellent. The sales price estimation model uses a regression algorithm. According to the R-Squared value, the Light Gradient Boosting Machine performs best in this model.

전동차용 Blow-Down HVAC 덕트 시스템의 유동 균일도 향상을 위한 수치적 연구 (Numerical Study to Improve the Flow Uniformity of Blow-Down HVAC Duct System for a Train)

  • 김준형;노주현
    • 한국유체기계학회 논문집
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    • 제19권1호
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    • pp.18-23
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    • 2016
  • A HVAC(Heating Ventilation and Air Conditioning) is adapted to increase the comfort of the cabin environment for train. The train HVAC duct system has very long duct and many outlets due to the shape of a train set. the duct cross section shape is limited by a roof structure and equipments. Therefore, the pressure distribution and flow uniformity is an important performance indicator for the duct system. In this study, the existing blow down type HVAC duct system for a train was supplemented to improve the flow uniformity by applying a design method combining design of experiment (DOE) with numerical analysis. The design variables and the test sets were selected and the performance for each test set was evaluated using CFD(Computational Fluid Dynamics). The influence of each design variable on the system performance was analysed based on the results of the performance evaluation on the test sets. Furthermore, the optimized model, whose the flow uniformity was improved was produced using the direct optimization(gradient-based method). Finally, the performance of the optimized model was evaluated using numerical analysis, and it was confirmed that its flow uniformity has indeed improved.

Assessment of computational performance for a vector parallel implementation: 3D probabilistic model discrete cracking in concrete

  • Paz, Carmen N.M.;Alves, Jose L.D.;Ebecken, Nelson F.F.
    • Computers and Concrete
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    • 제2권5호
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    • pp.345-366
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    • 2005
  • This work presents an assessment of the computational performance of a vector-parallel implementation of probabilistic model for concrete cracking in 3D. This paper shows the continuing efforts towards code optimization as reported in earlier works Paz, et al. (2002a,b and 2003). The probabilistic crack approach is based on the direct Monte Carlo method. Cracking is accounted by means of 3D interface elements. This approach considers that all nonlinearities are restricted to interface elements modeling cracks. The heterogeneity governs the overall cracking behavior and related size effects on concrete fracture. Computational kernels in the implementation are the inexact Newton iterative driver to solve the non-linear problem and a preconditioned conjugate gradient (PCG) driver to solve linearized equations, using an element by element (EBE) strategy to compute matrix-vector products. In particular the paper analyzes code behavior using OpenMP directives in parallel vector processors (PVP), such as the CRAY SV1 and CRAY T94. The impact of the memory architecture on code performance, and also some strategies devised to circumvent this issue are addressed by numerical experiment.

TWINSPAN과 DCCA Ordination에 의한 백두산(白頭山) 삼림군집(森林群集)의 분석(分析) (The Analysis on the Forest Community in Mt. Paekdu by Two-Way Indicator Species Analysis and Detrended Canonical Correspondence Analysis Ordination)

  • 송호경;김성덕
    • 한국산림과학회지
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    • 제81권4호
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    • pp.310-319
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    • 1992
  • 백두산(白頭山) 삼림군집(森林群集)의 구조(構造) 및 종(種) 조성(組成)과 입지(立地)와의 관계(關係)를 구명(究明)하기 위하여 TWINSPAN과 DCCA를 사용(使用)여 분석(分析)한 결과(結果)는 다음과 같다. 중요치(重要値)가 높은 종(種)은 해발(海拔) 1,700m-1,950m 대(帶)에 서 Betula ermanii, Larix olgensis, Abies nephrolepis, Picea jezoensis 등(等)의 순(順)이고, 해발(海拔) 1,300-1,500m 대(帶)에서 Abies nephrolepis, Pinus koraiensis, Larix olgensis, Betula mandshurica, Picea jezoensis 등(等)의 순(順)이다. 삼림토양(森林土壤)은 유효인산량(有效燐酸量)에서 7ppm-1,153ppm으로 과대치를 보이고 있다. 백두산(白頭山)의 삼림군집(森林群集)과 해발고(海拔高)와의 관계를 살펴보면, 해발고(海拔高)가 높은 곳에서 낮은 곳으로 덤불오리나무, 사스래나무, 만주잎갈나무, 분비나무, 가문비나무, 잣나무, 신갈나무군집(群集) 등(等)의 순(順)으로 분포(分布)하고 있다. 사스래나무군락(群落)이 우점(優占)한 곳을 중심(中心)으로 사스래나무, 만주잎갈나무, 분비나무 사이의 관계(關係)을 살펴보면, 사스래나무군집(群集)은 해발고(海拔高)가 가장 높은 곳에, 사스래나무-만주잎갈나무군집(群集)은 유기물함량(有機物含量)과 $K^+$의 함량이 많은 곳에, 분비나무군집(群集)은 $Mg^{{+}{+}}$$Ca^{{+}{+}}$의 함량(含量)이 많은 곳에 주로 분포(分布)하고 있다. 군집(群集) 분포(分布)에 영향(影響)을 미치는 주요(主要) 환경요인(環境要因)은 해발고(海拔高)이다.

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Influence of Seasonal Forcing on Habitat Use by Bottlenose Dolphins Tursiops truncatus in the Northern Adriatic Sea

  • Bearzi, Giovanni;Azzellino, Arianna;Politi, Elena;Costa, Marina;Bastianini, Mauro
    • Ocean Science Journal
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    • 제43권4호
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    • pp.175-182
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    • 2008
  • Bottlenose dolphins are the only cetaceans regularly observed in the northern Adriatic Sea, but they survive at low densities and are exposed to significant threats. This study investigates some of the factors that influence habitat use by the animals in a largely homogeneous environment by combining dolphin data with hydrological and physiographical variables sampled from oceanographic ships. Surveys were conducted year-round between 2003 and 2006, totalling 3,397 km of effort. Habitat modelling based on a binary stepwise logistic regression analysis predicted between 81% and 93% of the cells where animals were present. Seven environmental covariates were important predictors: oxygen saturation, water temperature, density anomaly, gradient of density anomaly, turbidity, distance from the nearest coast and bottom depth. The model selected consistent predictors in spring and summer. However, the relationship (inverse or direct) between each predictor and dolphin presence varied among seasons, and different predictors were selected in fall. This suggests that dolphin distribution changed depending on seasonal forcing. As the study area is relatively uniform in terms of bottom topography, habitat use by the animals seems to depend on complex interactions among hydrological variables, caused primarily by seasonal change and likely to determine shifts in prey distribution.

디스크 브레이크와 패드의 접촉을 고려한 벤틸레이티드 디스크 브레이크의 열적거동에 관한 연구 (Thermal Behavior of Ventilated Disc Brakes Considering Contact Between Disc and Pad)

  • 마정범;이봉구
    • 한국생산제조학회지
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    • 제23권3호
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    • pp.259-265
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    • 2014
  • When the brakes of a vehicle are applied, large amounts of heat are generated on the surfaces of the brake discs owing to friction between the discs and the brake pads. A high temperature gradient on the disc surfaces leads to thermal deformation and severe disc abrasion. Ultimately, the thermal deformation and disc wear give rise to a thermal judder phenomenon, which has a major effect on the stability of the vehicle. To investigate and propose a solution to these problems, thermoelastic instabilities under applied thermal and mechanical loads were analyzed using the commercial finite element package ANSYS by considering the contact surfaces between the discs and pads. Direct-contact three-dimensional finite elements between the discs and pads were applied to investigate the disc friction temperature, thermal deformation, and contact stress so that the thermal judder phenomenon on the surface of the disc could be predicted.

$Zn_4SnSe_6:Co^{2+}$ 단결정의 성장방법에 관한 연구 (The Single Crystal Growth Method of undoped and Co-doped $Zn_4SnSe_6$)

  • 김덕태;박광호;현승철;방태환;김남오;김형곤
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2006년도 영호남 합동 학술대회 및 춘계학술대회 논문집 센서 박막 기술교육
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    • pp.27-30
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    • 2006
  • In this paper, the undoped and Co-doped $Zn_4SnSe_6$ single crystals grown by the chemical transporting reaction(CTR) method using iodine as a transporting agent are investigated. For the crystal growth, the temperature gradient of the CTR furnace was kept at $680^{\circ}C$ for the source zone and at $780^{\circ}C$ for the growth zone for 7days. It was found from the analysis of x-ray diffraction that the $Zn_4SnSe_6$ and $Zn_4SnSe_6Co^{2+}$ compounds have a monoclinic structure. The direct optical energy band gap of the $Zn_4SnSe_6$ and $Zn_4SnSe_6Co^{2+}$ single crystals at 300K were found to be 2.146eV and 2.042eV.

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