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Specific Detection of Listeria monocytogenes in Foods by a Polymerase Chain Reaction (PCR에 의한 식품으로부터 Listeria monocytogenes의 특이적 검출)

  • Shin, Soon-Young;Koo, Young-Jo;Kim, Wang-June
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1628-1634
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    • 1999
  • The polymerase chain reaction (PCR) for the sensitive and specific detection of Listeria monocytogenes was employed by using LM 1 and LM 2 primers which were based on the listeriolysin O gene. The direct use of cell suspension as DNA template, without DNA extraction or lysis step, was suitable and specific enough to detect L. monocytogenes at the level of $10^2$ CFU or less per PCR for the pure culture and milk sample, however, the detection sensitivity became blunt for other food samples such as kimchi and chicken. The nested PCR, in which L-1 and L-2 (both designed from listeriolysin O gene) were employed as inner primers, was specific for detecting L. monocytogenes and enhanced the detection limit by 10 times. The PCR using LM 1 and LM 2 primers was very effective to detect L. monocytogenes from foods in terms of the specificity and time consumed, i. e. within $4{\sim}8\;hrs$ (nested PCR).

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Monitoring of the Optical Purity for Levothyroxine Sodium in Pharmaceuticals Using Crown Ether Derived Chiral Columns (크라운 에테르에서 유도된 키랄 컬럼을 사용한 레보티록신 나트륨 의약품의 광학순도 모니터링)

  • Jeon, So-Hee;Lee, Won-Jae
    • KSBB Journal
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    • v.25 no.5
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    • pp.449-452
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    • 2010
  • L-Thyroxine possessing a chiral center, the naturally occurring thyroid hormone has been used for the treatment of thyroid dysfunctions and marketed as levothyroxine (L-thyroxine) sodium salt. In this study, after extraction of levothyroxine tablet as a pre-treatment process, direct enantiomer separation of thyroxine on crown ether derived chiral columns for determination of optical purity was performed using reversed mobile phase with acid additive. The chromatographic method developed in this study was applied in the determination of optical purity of several current domestic and foreign commercialized levothyroxine tablets. Optical purity values of these commercialized L-thyroxine sodium tablets except one were higher than 99 percents.

Influence of autoclave sterilization procedures on the cyclic fatigue resistance of heat-treated nickel-titanium instruments: a systematic review

  • Silva, Emmanuel Joao Nogueira Leal;Zanon, Mayara;Hecksher, Fernanda;Belladonna, Felipe Goncalves;de Vasconcelos, Rafaela Andrade;da Silva Fidalgo, Tatiana Kelly
    • Restorative Dentistry and Endodontics
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    • v.45 no.2
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    • pp.25.1-25.12
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    • 2020
  • Objectives: This systematic review evaluated the influence of autoclave sterilization procedures on the cyclic fatigue resistance of heat-treated nickel-titanium (NiTi) instruments. Materials and Methods: A systematic search without restrictions was conducted in the following electronic databases: PubMed, Scopus, Web of Science, ScienceDirect, Cochrane, and Open Grey. The hand search was also performed in the main endodontic journals. The eligible studies were submitted to the methodological assessment and data extraction. Results: From 203 abstracts, a total of 10 articles matched the eligible criteria. After reading the full articles, 2 were excluded because of the absence of the heat-treated instruments in the experimental design and 3 due to the lack of a control group using heat-treated instruments without autoclave sterilization. From the 5 included studies, 1 presented a low risk of bias, 3 presented moderate and 1 high risk. It was observed heterogeneous findings in the included studies, with autoclave sterilization cycles increasing, decreasing or not affecting the cyclic fatigue life of heat-treated NiTi instruments. However, the retrieved studies evaluating the cyclic fatigue resistance of endodontic instruments presented different protocols and assessing outcomes, this variability makes the findings less comparable within and also between groups and preclude the establishment of an unbiased scientific evidence base. Conclusions: Considering the little scientific evidence and considerable risk of bias, it is still possible to conclude that autoclave sterilization procedures appear to influence the cyclic fatigue resistance of heat-treated NiTi instruments.

Face Recognition Evaluation of an Illumination Property of Subspace Based Feature Extractor (부분공간 기반 특징 추출기의 조명 변인에 대한 얼굴인식 성능 분석)

  • Kim, Kwang-Soo;Boo, Deok-Hee;Ahn, Jung-Ho;Kwak, Soo-Yeong;Byun, Hye-Ran
    • Journal of KIISE:Software and Applications
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    • v.34 no.7
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    • pp.681-687
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    • 2007
  • Face recognition technique is very popular for a personal information security and user identification in recent years. However, the face recognition system is very hard to be implemented due to the difficulty where change in illumination, pose and facial expression. In this paper, we consider that an illumination change causing the variety of face appearance, virtual image data is generated and added to the D-LDA which was selected as the most suitable feature extractor. A less sensitive recognition system in illumination is represented in this paper. This way that consider nature of several illumination directions generate the virtual training image data that considered an illumination effect of the directions and the change of illumination density. As result of experiences, D-LDA has a less sensitive property in an illumination through ORL, Yale University and Pohang University face database.

In vivo assessment of accuracy of Propex II, Root ZX II, and radiographic measurements for location of the major foramen

  • Tampelini, Fernanda Garcia;Coelho, Marcelo Santos;de Azevedo Rios, Marcos;Fontana, Carlos Eduardo;Rocha, Daniel Guimaraes Pedro;Pinheiro, Sergio Luiz;da Silveira Bueno, Carlos Eduardo
    • Restorative Dentistry and Endodontics
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    • v.42 no.3
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    • pp.200-205
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    • 2017
  • Objectives: The aim of this in vivo study was to assess the accuracy of 2 third-generation electronic apex locators (EALs), Propex II (Dentsply Maillefer) and Root ZX II (J. Morita), and radiographic technique for locating the major foramen (MF). Materials and Methods: Thirty-two premolars with single canals that required extraction were included. Following anesthesia, access, and initial canal preparation with size 10 and 15 K-flex files and SX and S1 rotary ProTaper files, the canals were irrigated with 2.5% sodium hypochlorite. The length of the root canal was verified 3 times for each tooth using the 2 apex locators and once using the radiographic technique. Teeth were extracted and the actual WL was determined using size 15 K-files under a ${\times}25$ magnification. The Biostat 4.0 program (AnalystSoft Inc.) was used for comparing the direct measurements with those obtained using radiographic technique and the apex locators. Pearson's correlation analysis and analysis of variance (ANOVA) were used for statistical analyses. Results: The measurements obtained using the visual method exhibited the strongest correlation with Root ZX II (r = 0.94), followed by Propex II (r = 0.90) and Ingle's technique (r = 0.81; p < 0.001). Descriptive statistics using ANOVA (Tukey's post hoc test) revealed significant differences between the radiographic measurements and both EALs measurements (p < 0.05). Conclusions: Both EALs presented similar accuracy that was higher than that of the radiographic measurements obtained with Ingle's technique. Our results suggest that the use of these EALs for MF location is more accurate than the use of radiographic measurements.

Development of the software transforming a transportation analysis network from GIS (GIS로부터 교통분석용 네트워크를 생성하는 소프트웨어의 개발)

  • 성낙문;조범철;이창렬
    • Journal of Korean Society of Transportation
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    • v.20 no.7
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    • pp.69-76
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    • 2002
  • In conducting studies related to the national base networks, it is very important to construct a simulation network. This research provides an algorithm to construct the simulation network from the digital transportation map which is constructed based on the National Geographic Information System(NGIS). The algorithm consists of three functions(extraction of networks from transportation digital map, transform of the derived network into one suitable to transportation simulation model, and inspection of errors in the network). The direct derivation of a simulation network from GIS enables to enhance the reliability of an analysis related to a transportation facility investment as well as to reduce cost and time. In this research, Emme/2 which is generally accepted transportation planning fields is adapted for the target system. However, this algorithm will be extended to other simulation models such as Satong-Paldal which is the only transportation simulation model developed in Korea, and Tranplan in the near future.

The Screening and Pattern Comparison of Organic Acids in 3 Kinds of Medicinal Herbal Extracts (3가지 약용 허브 추출물에 함유된 유기산 검색 및 조성 비교)

  • Chung, Ha-Yull;Jung, Do-Hyun;Park, Young-Joon
    • Korean Journal of Food Science and Technology
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    • v.32 no.5
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    • pp.997-1001
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    • 2000
  • The organic acids in 3 kinds of medicinal herbal extracts were screened and compared each other according to their organic acid contents by an efficient gas chromatographic method. It involves solid-phase extraction of organic acids using Chromosorb P with subsequent conversion to stable tert-butyldimethysilyl derivatives for the direct analysis by capillary column gas chromatography and gas chromatography-mass spectrometry. Total of 24 organic acids were reproducibly identified from 3 kinds of herbal extracts. When the GC profiles were simplified to their retention index spectra, characteristic patterns were obtained for each herb sample. As expected, three kinds of herbal extracts showed three distinct patterns.

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Development of Extraction Technique for Irrigated Area and Canal Network Using High Resolution Images (고해상도 영상을 이용한 농업용수 수혜면적 및 용배수로 추출 기법 개발)

  • Yoon, Dong-Hyun;Nam, Won-Ho;Lee, Hee-Jin;Jeon, Min-Gi;Lee, Sang-Il;Kim, Han-Joong
    • Journal of The Korean Society of Agricultural Engineers
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    • v.63 no.4
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    • pp.23-32
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    • 2021
  • For agricultural water management, it is essential to establish the digital infrastructure data such as agricultural watershed, irrigated area and canal network in rural areas. Approximately 70,000 irrigation facilities in agricultural watershed, including reservoirs, pumping and draining stations, weirs, and tube wells have been installed in South Korea to enable the efficient management of agricultural water. The total length of irrigation and drainage canal network, important components of agricultural water supply, is 184,000 km. Major problem faced by irrigation facilities management is that these facilities are spread over an irrigated area at a low density and are difficult to access. In addition, the management of irrigation facilities suffers from missing or errors of spatial information and acquisition of limited range of data through direct survey. Therefore, it is necessary to establish and redefine accurate identification of irrigated areas and canal network using up-to-date high resolution images. In this study, previous existing data such as RIMS (Rural Infrastructure Management System), smart farm map, and land cover map were used to redefine irrigated area and canal network based on appropriate image data using satellite imagery, aerial imagery, and drone imagery. The results of the building the digital infrastructure in rural areas are expected to be utilized for efficient water allocation and planning, such as identifying areas of water shortage and monitoring spatiotemporal distribution of water supply by irrigated areas and irrigation canal network.

Possibility and Accuracy of Extracting Room Temperature Information from Mid-Infrared Sensor Satellite Images (중적외선 센서 위성 영상의 상온 온도 정보 추출 가능성 및 정확도)

  • Choi, SeokWeon;Seo, DooChun;Lee, DongHan
    • Journal of Space Technology and Applications
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    • v.1 no.3
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    • pp.356-363
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    • 2021
  • It was common knowledge in textbooks that images acquired using mid-infrared ray were not suitable for measuring temperature near room temperature. But a recent satellite image using a mid-infrared sensor show the possibility that the result measured using the mid-infrared sensor can also measure the temperature near room temperature. In this paper, the possibility and accuracy of extraction room temperature information from satellite images with mid-infrared sensors are reviewed. The mid-infrared satellite image reviewed in this paper showed the temperature of room temperature well, and regarding the reliability as an absolute value of the measured temperature, the effect of the heat transfer amount due to the direct reflection of sunlight on the surface and the effect of the infrared absorption amount absorbed in the atmosphere can be seen as a relatively small or constant value. However, the problem of uncertainty in the radiation coefficient due to physical properties, which is the limit of the non-contact thermometer, remained a problem to be solved.

Long Song Type Classification based on Lyrics

  • Namjil, Bayarsaikhan;Ganbaatar, Nandinbilig;Batsuuri, Suvdaa
    • Journal of Multimedia Information System
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    • v.9 no.2
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    • pp.113-120
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    • 2022
  • Mongolian folk songs are inspired by Mongolian labor songs and are classified into long and short songs. Mongolian long songs have ancient origins, are rich in legends, and are a great source of folklore. So it was inscribed by UNESCO in 2008. Mongolian written literature is formed under the direct influence of oral literature. Mongolian long song has 3 classes: ayzam, suman, and besreg by their lyrics and structure. In ayzam long song, the world perfectly embodies the philosophical nature of world phenomena and the nature of human life. Suman long song has a wide range of topics such as the common way of life, respect for ancestors, respect for fathers, respect for mountains and water, livestock and animal husbandry, as well as the history of Mongolia. Besreg long songs are dominated by commanded and trained characters. In this paper, we proposed a method to classify their 3 types of long songs using machine learning, based on their lyrics structures without semantic information. We collected lyrics of over 80 long songs and extracted 11 features from every single song. The features are the name of a song, number of the verse, number of lines, number of words, general value, double value, elapsed time of verse, elapsed time of 5 words, and the longest elapsed time of 1 word, full text, and type label. In experimental results, our proposed features show on average 78% recognition rates in function type machine learning methods, to classify the ayzam, suman, and besreg classes.