• 제목/요약/키워드: Direct extraction

검색결과 340건 처리시간 0.026초

A Reliable "Direct from Field" PCR Method for Identification of Mycorrhizal Fungi from Associated Roots

  • Kuhnann, Christoph;Kim, Seak-Jin;Lee, Sang-Sun;Harms, Carsten
    • Mycobiology
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    • 제31권4호
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    • pp.196-199
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    • 2003
  • A very reliable and specific method for the identification of fungi in ectotrophic mycorrhizal symbiosis was developed using a specific PCR assay based on the amplification of the ITS1 region. To obtain specific data, an ITS-diagnostic assay was carried out that reveals genera and species specific sequences. Here, an application of one method is presented, which covers the identification of pure mycelia, basidiocarps as well as mixed samples such as ectomycorrhizal roots that were mingled with remains of the host plant. For this purpose a protocol was established that allowed the extraction of DNA from single mycorrhizal roots. In order to perform a specific ITS analysis we generated a new ITS-primer(ITS8) by a multiple alignment of five different genera and species of mycorrhizal fungi. The utilization of ITS1 and ITS8 resulted in specific PCR amplicons, which were characterized by sequencing without purification steps, even when the template DNA was associated with roots.

Extraction of rational functions by forced vibration method for time-domain analysis of long-span bridges

  • Cao, Bochao;Sarkar, Partha P.
    • Wind and Structures
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    • 제16권6호
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    • pp.561-577
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    • 2013
  • Rational Functions are used to express the self-excited aerodynamic forces acting on a flexible structure for use in time-domain flutter analysis. The Rational Function Approximation (RFA) approach involves obtaining of these Rational Functions from the frequency-dependent flutter derivatives by using an approximation. In the past, an algorithm was developed to directly extract these Rational Functions from wind tunnel section model tests in free vibration. In this paper, an algorithm is presented for direct extraction of these Rational Functions from section model tests in forced vibration. The motivation for using forced-vibration method came from the potential use of these Rational Functions to predict aerodynamic loads and response of flexible structures at high wind speeds and in turbulent wind environment. Numerical tests were performed to verify the robustness and performance of the algorithm under different noise levels that are expected in wind tunnel data. Wind tunnel tests in one degree-of-freedom (vertical/torsional) forced vibration were performed on a streamlined bridge deck section model whose Rational Functions were compared with those obtained by free vibration for the same model.

Bio-oil production using residual sewage sludge after lipid and carbohydrate extraction

  • Supaporn, Pansuwan;Ly, Hoang Vu;Kim, Seung-Soo;Yeom, Sung Ho
    • Environmental Engineering Research
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    • 제24권2호
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    • pp.202-210
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    • 2019
  • In order to maximize the utilization of sewage sludge, a waste from wastewater treatment facility, the residual sewage sludge generated after lipid and carbohydrate extraction for biodiesel and bioethanol production was used to produce bio-oil by pyrolysis. Thermogravimetric analysis showed that sludge pyrolysis mainly occurred between 200 and $550^{\circ}C$ (with peaks formed around 337.0 and $379.3^{\circ}C$) with the decomposition of the main components (carbohydrate, lipid, and protein). Bio-oil was produced using a micro-tubing reactor, and its yield (wt%, g-bio-oil/g-residual sewage sludge) increased with an increase in the reaction temperature and time. The maximum bio-oil yield of 33.3% was obtained after pyrolysis at $390^{\circ}C$ for 5 min, where the largest amount of energy was introduced into the reactor to break the bonds of organic compounds in the sludge. The main components of bio-oil were found to be trans-2-pentenoic acid and 2-methyl-2-pentenoic acid with the highest selectivity of 28.4% and 12.3%, respectively. The kinetic rate constants indicated that the predominant reaction pathway was sewage sludge to bio-oil ($0.1054min^{-1}$), and subsequently to gas ($0.0541min^{-1}$), rather than the direct conversion of sewage sludge to gas ($0.0318min^{-1}$).

유리소재의 Pb 및 Cd 분석에 관한 연구 (Analytic study on lead and cadmium in glass materials)

  • 최철호;고재권
    • 분석과학
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    • 제20권1호
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    • pp.41-48
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    • 2007
  • 유리소재 중의 미량의 납 및 카드뮴을 Na-DDTC를 가하여 Pb- 및 Cd-DDTC 착물을 형성시켜 유리 matrix 원소들로 부터 분리한 다음 MIBK로 추출하여 ICP-AES로 분석하였다. 유리 기질성분인 Na, Si, Mg 그리고 Ca 화합물 등은 미량의 납 및 카드뮴 정량에 방해작용을 하므로 직접 분석할 수 없으므로 용매추출법에 의해 Pb 및 Cd를 효율적으로 분리할 수 있었다. Pb 및 Cd 화합물이 첨가된 시험기준 시료와 NIST SRM 1412에 대한 용매추출실험에서 matrix의 영향을 받지 않고 정량하였다.

가시광선 조사에 의한 이미지 추출법을 이용한 막 오염 모니터링 연구 (Study for Membrane Fouling Monitering Using Image Extraction by Visible Light Irradiation)

  • 박아름이;서미래;남승은;김범식;박호범;김인철;박유인
    • 멤브레인
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    • 제21권2호
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    • pp.171-176
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    • 2011
  • 분리막을 이용한 수처리 공정에서 유입 수에 함유된 부유물질이나 기타 오염물질이 막 표면 또는 내부에 축적 흡착 등의 막 오염현상으로 인해 막 성능 감소와 함께 막 분리 공정에 큰 영향을 미치게 된다. 본 연구에서는 막 표면에서의 막 오염현상을 실시간으로 모니터링 할 수 있는 기술을 연구하였다. 투명한 오염물질에 의한 분리막 표면 오염을 측정하기 위해 막 표면에 360 nm 파장의 가시광선을 조사하여 이미지를 R. G. B 값으로 추출하여 막의 오염현상을 실시간으로 모니터링 하였다. 추출된 이미지 중 400~499 nm 파장영역인 B 값이 가장 강도가 강하게 나타났다. 막 오염정도의 변화를 이미지의 강도 차이로 관찰함으로써 실시간 분석이 가능함을 확인하였다.

Multiple brain abscesses treated by extraction of the maxillary molars with chronic apical lesion to remove the source of infection

  • Jung, Ki-Hyun;Ro, Seong-Su;Lee, Seong-Won;Jeon, Jae-Yoon;Park, Chang-Joo;Hwang, Kyung-Gyun
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제41권
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    • pp.25.1-25.5
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    • 2019
  • Background: Brain abscess is a life-threatening condition that occurs due to complications during a neurosurgical procedure, direct cranial trauma, or the presence of local or distal infection. Infection in the oral cavity can also be considered a source of brain abscess. Case presentation: A 45-year-old male patient was transported with brain abscess in the subcortical white matter. Navigation-guided abscess aspiration and drainage was performed in the right mid-frontal lobe, but the symptoms continued to worsen after the procedure. A panoramic radiograph showed alveolar bone resorption around the maxillary molars. The compromised maxillary molars were extracted under local anesthesia, and antibiotics were applied based on findings from bacterial culture. A brain MRI confirmed that the three brain abscesses in the frontal lobe were reduced in size, and the patient's symptoms began to improve after the extractions. Conclusion: This is a rare case report about multiple uncontrolled brain abscesses treated by removal of infection through the extraction of maxillary molars with odontogenic infection. Untreated odontogenic infection can also be considered a cause of brain abscess. Therefore, it is necessary to recognize the possibility that untreated odontogenic infection can lead to serious systemic inflammatory diseases such as brain abscess. Through a multidisciplinary approach to diagnosis and treatment, physicians should be encouraged to consider odontogenic infections as a potential cause of brain abscesses.

A case report of a long-term abandoned torn lingual nerve injury repaired by collagen nerve graft induced by lower third molar extraction

  • Fujita, Shigeyuki;Mizobata, Naoki;Nakanishi, Takashi;Tojyo, Itaru
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제41권
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    • pp.60.1-60.6
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    • 2019
  • Background: The lingual nerve plays an important role in multiple functions, including gustatory sensation and contact sensitivity and thermosensitivity. Misdiagnosed conservative treatments for serious lingual nerve (LN) injuries can induce the patient to serious mental disability. After continuous observation and critical diagnosis of the injury, in cases involving significant disruption of lingual nerve function, microneurosurgical reconstruction of the nerve is recommended. Direct anastomosis of the torn nerve ends without tension is the recommended approach. However, in cases that present significant gaps between the injured nerve ends, nerve grafts or conduits (tubes of various materials) are employed. Recently, various reconstruction materials for peripheral nerves were commercially offered especially in the USA, but the best method and material is still unclear in the world. There currently exists no conventional protocol for managing LN neurosensory deficiency in regard to optimal methods and the timing for surgical repair. In Japan, the allograft collagen nerve for peripheral nerves reconstruction was permitted in 2017, and we tried to use this allograft nerve and got a recommendable result. Case presentation: This report is a long-term abandoned torn LN reconstructed with allograft nerve induced by the lower third molar extraction. Conclusions: In early sick period, with the exact diagnosis, the LN disturbance should be managed. In a serious condition, the reconstruction with allograft nerve is one of the recommendable methods.

한대의 카메라에 기반한 직육면체의 부피 계측 방법 (A Single Camera based Method for Cubing Rectangular Parallelepiped Objects)

  • 원종운;정윤수;김우섭;유광훈;이용준;박길흠
    • 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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    • 제8권5호
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    • pp.562-573
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    • 2002
  • 본 논문에서는 소포 및 택배와 같은 패키지(package)의 효과적인 취급(handling)을 위한 직육면체의 부피 계측 방법을 제안한다. 제안된 방법은 한대의 카메라와 직육면체의 특성을 이용하여 실시간으로 부피 계측을 수행한다. 부피 계측을 위한 전처리 과정에서, 제안된 방법은 직육면체의 외곽 선분 정보를 검출하고, 이러한 선분들의 교차점을 3D 물체의 꼭지점으로 추출/인식하여, 물체의 부피를 계산한다. 제안된 방법은 선분 정보를 이용하여 꼭지점을 추출함으로써, 꼭지점을 직접 추출하는 경우에 비하여 카메라의 블러링 효과에 비교적 강인한 특성을 나타내며, 물체의 방향을 고려함으로써 견실한 부피계측 결과를 나타낸다. 실험의 결과를 통하여 제안된 방법이 직육면체 물체의 실시간 부피 계산에 효과적으로 사용될 수 있음이 보여진다.

함아연 폐기물로부터 습식법에 의한 고순도 ZnO 분말의 제조 (Preparation of High Purity ZnO Powder from zinc-bearing waste by the Hydrometallurgical Process)

  • 이재천;이강인;유효신
    • 자원리싸이클링
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    • 제1권1호
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    • pp.58-68
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    • 1992
  • 아연을 함유하고 있는 산업폐기물로부터 첨단산업의 소재원료인 미립의 고순도 ZnO 분말을 아연제련의 용매추출 공정에서 직접 합성할 수 있는 방법을 개발하고자 하였다. 아연 함량이 55%인 선재산업의 폐기물을 황산으로 침출하여 아연을 추출하였다. 함아연 용액에서 철분을 침전시켜 제거한 후 D2EHPA를 추출제로 사용하는 용매추출에 의해 아연이온을 유기상으로 추출하여 정제한 뒤 oxalic acid와 같은 침전제를 사용하여 유기상의 아연 이온을 precipitation stripping 법으로 탈거, 침전시켜 $ZnC_2O_4$ 분말을 합성하였으며 이를 해소하여 99.9% 이상의 ZnO 분말을 제조하였다. 황산농도, 침출시간, 황액농도가 아연의 침출율에 미치는 영향과 용매추출시 용액의 산도에 따른 아연과 불순물의 추출거동을 조사하여 최적정제조건을 얻었다. 또한 oxalic acid의 농도, 온도, 계면활성제의첨가, 침전시간 등이 생성된 $ZnC_2O_4$ 분말의 형상과 입자크기 및 분포에 미치는 영향에 대해 조사하였다.

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도심인공호 퇴적물의 총중금속, 용존중금속, 생물이용성 중금속의 연관성 규명 (Examining the Interrelation of Total, Soluble, and Bioavailable Metals in the Sediments of Urban Artificial Lakes)

  • 백용욱;안윤주
    • 생태와환경
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    • 제41권1호
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    • pp.66-72
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    • 2008
  • 대도시의 기본 생활수준 향상을 위해 건립된 인공호는 지역적 환경요인에 의한 퇴적물 중금속 오염이 우려된다. 본 연구의 퇴적물 조사대상인 도심의 소형인공호들은 저수용량이 적고, 체류시간이 길며, 도시대기오염으로 인한 침적물낙하와 함께 호수에 유입될 수 있는 중금속이 최종적으로 퇴적물에 축적된다. 본 연구에서는 도시지역에 조성된 인공호를 대상으로 퇴적물매체에 축적된 총중금속, 용존중금속, 그리고 생물이용성 중금속의 상호연관성을 규명하고자 시도하였다. 생물이용량은 약전해질추출법(Weak-Electrolyte Extraction, WEE)에 의해 측정하였다. 도심인공호 전체 조사지역에 대한 퇴적물의 총중금속 검출빈도는 아연, 구리, 크롬, 니켈, 비소, 카드뮴 순으로 확인되었고, 총량에 대한 검출은 아연이 가장 높은 수치를 나타내었다. 총중금속에 대한 생물이용성 중금속의 비율은 중금속별, 측정지역별로 매우 상이하였으며 전반적으로 중금속의 생물이용성은 구리 (Cu)가 높은 것으로 나타났다. 높은 생물이용성을 나타낸 구리의 경우 기존독성연구 결과와 비교한 결과 높은 생태독성을 나타내고 있어 본 연구에서 예측한 현상을 지지하고 있으나, 차후 생물검정법(Bioassay)을 통한 검증이 필요할 것으로 사료된다.