• Journal of Industrial Technology
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• v.28 no.A
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• pp.141-147
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• 2008

Several contaminated bacteria such as Lactobacillus brevis and Pediococcus damnosus in beer production cause beer spoilage by producing off flavours and turbidity. Detection of these organisms is complicated by the strict anaerobic conditions and lengthy incubation times required for their cultivation, consequently there is a need for more rapid detection methods. Recently, two contaminated strains were isolated from vessel of beer production and identified as Lactobacillus species by API kit identificaton as well as 16S-23S ITS sequencing analyses. Two isolated strains were named as Lactobacillus sp. HLA1 and Lactobacillus HLB2, respectively. A polymerase chain reaction (PCR) method was developed for the rapid and specific detection of Lactobacillus sp.. Two sets of primer pairs (HLA1-F/HLA1-R and HLB2-F/HLB2-R) were designed for the amplification of a 1576 base pair (bp) fragment of the HLA1 16S-23S rRNA gene and 1888 bp fragement of the HLB2 16S-23S rRNA. Amplified PCR products were highly specific to detect corresponding bacteria when other contaminated strains were used as PCR templates. However, detection of both strains were limited when $100{\mu}{\ell}$ of cultured samples were mixed with $100m{\ell}$ of beer sample in arbitrary manner. The sensitivity of the assay still needs to be improved for direct detection of the small amounts of bacteria present in beer.

• Journal of Microbiology and Biotechnology
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• v.9 no.2
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• pp.184-190
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• 1999

The use of the polymerase chain reaction (PCR) method was described using two sets of primers based on the ceuN gene (JEJ 1 and JEJ 2) which encodes a protein involved in siderophore transport and 16S rRNA gene (pA and pB) for the sensitive and specific detection of enteropathogen Campylobacter jejuni. Six oligonucleotides were utilized in an amplification experiment and PCR products of predicted sizes were generated from whole cells and boiled cell lysates at the same intensity. Two sets of the primer pairs, JEJ and pAB, were specific enough for all C. jejuni strains tested for the direct use of whole cells without DNA extraction or lysis steps. In the PCR using the pAB primer pair, the detection limit, as determined by the ethidium bromide staining of the amplification products on agarose gels, was at the level of $10^1$ bacteria cells or less in both the pure culture and artificially inoculated milk and chicken enrichment samples, whereas the detection limit with the JEJ primer pair was relatively low, i.e. $10^3$ cells or more in the same PCR samples. The PCR method using either a primer JEJ or pAB was both repeatable and specific for the detection of C. jejuni in food. This method is simply completed within 4 h.

• Journal of Sensor Science and Technology
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• v.32 no.2
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• pp.67-74
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• 2023

A microfluidic paper-based analytical device (µPAD) is proposed for the selective detection of ammonia in water by using the modified Berthelot reagent and a fluidic channel consisting of hollow paper. The modified Berthelot reagents were uniformly dispersed in cyclohexane and then immobilized in a detection zone of the µPAD. The loading position of the reagents and the type of a sample flow channel were optimized to achieve a sensitive ammonia detection within a short analytical time. The NH₃ µPAD exhibits a linear colorimetric response to the concentration of ammonia dissolved in water in the range of 1-100 mg L^-1, and its limit-of-detection is 1.75 mg L^-1. In addition, the colorimetric response was not influenced by the addition of 100 mg L^-1 nitrogen containing compounds (sodium nitrate, sodium nitrite, uric acid, hydroxylamine, butylamine, diethylamine) or inorganic salts (NaCl, Na₂HPO₄), presenting the enough selectivity in the detection of water-dissolved ammonia against possible interferents.

• Journal of Agricultural Extension & Community Development
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• v.7 no.2
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• pp.289-293
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• 2000

The way society works in the 21st century differs from that of 20th century, since the people are brought-up to speed regarding current technologies. The www.aflos.pe.kr site and direct e-mailing system were very useful in delivering floricultural information to extension educators, producers, and variety of individuals. The author’s one year experience indicated that extension educators and farmers are receptive to internet technologies, and extension educators have increased the knowledge base of their clientele by responding through direct e-mails. The internet and direct e-mailing systems were popular and powerful way of transferring floricultural information, especially agricultural extension manpower were limited because of localization of extension educators by changing national status to local governments and decreased number of extension educators through government restructuring. The direct e-mailing to approximately 503 individuals resulted about $1{\sim}3%$ responses and the number of phone calls, however virus protection software for e-mail, internet, file servers and desktops to provide the integrated real-time detection of viruses were needed. For more effective operation of direct e-mailing in the future, more specified target groups and specialized organization such as perennials, bulbs, flowering potted plants. and cut flowers. At the same time, things that have worked for last century should not be replaced with new technology, specifically, the value in one-on-one meetings should not be replaced, but rather serve as a supplement.

• Clinical and Experimental Pediatrics
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• v.53 no.3
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• pp.397-407
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• 2010

Purpose : The F9 gene is known to be the causative gene for hemophilia B, but unfortunately the detection rate for restriction fragment length polymorphism-based linkage analysis is only 55.6%. Direct DNA sequencing can detect 98% of mutations, but this alternative procedure is very costly. Here, we conducted multiplex polymerase chain reactions (PCRs) and conformation sensitive gel electrophoresis (CSGE) to perform a screened DNA sequencing for the F9 gene, and we compared the results with direct sequencing in terms of accuracy, cost, simplicity, and time consumption. Methods : A total of 27 unrelated hemophilia B patients were enrolled. Direct DNA sequencing was performed for 27 patients by a separate institute, and multiplex PCR-CSGE screened sequencing was done in our laboratory. Results of the direct DNA sequencing were used as a reference, to which the results of the multiplex PCR-CSGE screened sequencing were compared. For the patients whose mutation was not detected by the 2 methods, multiplex ligation-dependent probe amplification (MLPA) was conducted. Results : With direct sequencing, the mutations could be identified from 26 patients (96.3%), whereas for multiplex PCRCSGE screened sequencing, the mutations could be detected in 23 (85.2%). One patient's mutation was identified by MLPA. A total of 21 different mutations were found among the 27 patients. Conclusion : Multiplex PCR-CSGE screened DNA sequencing detected 88.9% of mutations and reduced costs by 55.7% compared with direct DNA sequencing. However, it was more labor-intensive and time-consuming.

• Korean Journal of Food Science and Technology
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• v.38 no.2
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• pp.176-182
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• 2006

Sensitive and specific monoclonal antibody (MAb) was produced from hybridoma (1H11-5) obtained by fusion of myeloma cell (V653) and spleen cell isolated from mouse immunized sulfamthazine (SMZ)-HG-KLH. Direct competitive ELISA was developed for rapid detection of SMZ in milk samples using MAb against SMZ with optimized conditions between MAb and SMZ-HG-HRP conjugate, and applicable conditions for analysis of milk samples were established. Detection range of immunoassay was 0.1 to 100 ppb. Recoveries from spiked raw milk and processed milk samples averaged 82.1-120.7 and 82.1-97.1%, respectively.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.40 no.1
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• pp.31-38
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• 1991

A direct digital control technique of a current source using the phase-controlled rectifier is presented. A digital firing technique without sensing the line voltage is proposed. This scheme generates firing pulses directly from error signal between command and output voltage. Thus the phase detection transformers filters and zero-crossing detector are unnecessary. The synchronism is modeled and analized. Also a software synchronization algorithm is presented without a look up table and controls the system in real time with fast dynamic characteristics. Using the single-chip microprocessor 8097BH, the direct digital control is implemented with minimal hardware structure. Using the time-weighted performance index, the optimal discrete IPM control technique is also proposed to control the current of the PCR.

• Journal of environmental and Sanitary engineering
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• v.10 no.3
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• pp.35-44
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• 1995

This study was carried out in order to compare the methods for Salmonella spp. isolation from sewage and to settle the most appropriate conditions for that isolation. The direct enrichment method was more effective than the pre- enrichment method for Salmonella spp. isolation. The rate of detection was much higher when the specimens were enriched at $41.5^{\circ}C$ than when at $35^{\circ}C$. Usage of XLBG agar medium showed better results for Salmonella isolation than that of SS medium. It can be suggested that the most effective combination for Salmonella spp. isolation was the direct centrifugation(3,000 rpm 100m1)- direct enrichment($41.5^{\circ}$C)-usage of XLBG medium.

• Proceedings of the Korean Information Science Society Conference
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• 2012.06d
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• pp.135-136
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• 2012

윈도우기반 게임 프로그램의 엔진은 대부분 DirectX를 사용하고 있다. 이는 게임 프로세스를 탐지하는데에 있어서 수많은 게임의 이름을 알고 있지 않아도, DirectX의 사용여부로 게임 프로세스를 탐지할 수 있음을 의미한다. 본 논문은 유저모드 후킹 Windows Message Hooking과 Direct3D Hooking을 이용하여 게임 프로세스를 탐지하는 방법을 제안하고자 한다.

• The Journal of the Acoustical Society of Korea
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• v.2 no.1
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• pp.11-19
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• 1983

An irregulary formed chamber was designed and constructed to recognize the direct sound radiated from the sound source and the reflected sound from the walls of the chamber. The sound signal used was tone burst in the frequency response characteristics with the signal detection after transient effect. The direct wave, transient phenomena and the primary reflected sound could be asiily distinguished each other by measurements of the arrival time of the time difference. And also noise could be easily distinguished by the same method. The result obtained can be used in industries for automatic measurement of the sound pressure reponse characteristics with respect to frequencies.