• 제목/요약/키워드: Direct Method

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Direct Colorimetric Assay of Microcystin Using Protein Phosphatase

  • Oh, Hee-Mock;Lee, Seog-June;Kim, Jee-Hwan;Park, Chan-Sun;Yoon, Byung-Dae
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제5권6호
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    • pp.418-421
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    • 2000
  • A new direct colorimetric assay of microcystin in water and algal samples is proposed consisting of two procedures as follows: 1) the elimination of phosphorus in the sample and concentration of microcystin using a C(sub)18 cartridge, 2) the detection of the released phosphorus by the ascorbic acid method and determination of protein phosphatase (PP) inhibition by microcystin. The optimum amounts of phosphorylase ${\alpha}$ and PP-1 in 50 ${\mu}$L concentrated sample were 50$\mu\textrm{g}$/50${\mu}$L buffer and 1.0unit/50${\mu}$L buffer, respectively, for the best assay. The pH for the maximum activity of PP-1 was 8. The minimum detectable concentration for this method was about 0.02$\mu\textrm{g}$/L, which is sufficient to meet the proposed guideline level of 1$\mu\textrm{g}$ microcystin/L in drinking water. Consequently, it would seem that the proposed direct colorimetric assay using PP is a rapid, easy, and convenient method for the detection of microcystin in water and algal samples.

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영구 여기 조건이 불필요한 직접 적응 극배치 제어기의 설계 (Design of a Direct Adaptive Pole Placement Controller Without Persistency of Excitation)

  • 신강욱;최홍규;박준열
    • 한국통신학회논문지
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    • 제17권10호
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    • pp.1157-1163
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    • 1992
  • 비최소 위상 시스템에 대한 적응 제어 알고리즘의 발전은 제어 법칙에 있어서 발생되는 비정칙성(singularity)에 의하여 제한되어 왔다. 이러한 문제를 해결하기 위하여 직접 적응 제어방식과 간접 적응 제어방식을 사용하여 플랜트 신호에서의 영구 여기(persistency of excitation)를 유발시켜 플랜트의 실제값을 추정하기 위한 추정의 수렴성을 보장하는 방법과 간접 적응 제어방식을 사용하여 영구 여기 조건 없이 가제어성 필요 조건을 만족하도록 추정을 적절히 변형 시키는 방법이 연구되어 왔다. 본 논문에서는 직접 적응 제어방식을 사용하여 영구 여기 조건이 없어도 제어기의 파라메타 추정이 가능하도록 하였으며, 플랜트 파라메타의 추정없이 제어기의 파라메타를 직접 추정하도록하여 추정 알고리즘을 간소화하였다.

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고유수용성촉진법을 이용한 호흡운동이 경수 손상환자의 호흡기능에 미치는 영향 : 증례보고 (Using the PNF Approach to Improve Respiratory Function in Patients with Cervical Spinal Cord Injuries)

  • 송귀빈;김정빈
    • PNF and Movement
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    • 제12권2호
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    • pp.115-121
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    • 2014
  • Purpose: The study aimed to examine the influence of PNF direct and indirect breathing treatments for patients with cervical spinal cord injuries who had breathing problems. Methods: For each cervical spinal cord patient, force vital capacity (FVC), peak expiratory flow, maximum phonation time (MPT), rib cage width, and VAS were measured pre-intervention and four weeks after post-intervention. The indirect method and the direct method were used for interventions. We treated patients with the indirect method using scapular anterior depression pattern, bilateral extensor pattern with rhythmic initiation, and a combination of isotonic. We treated patients with the direct method, applying pressure on the sternum and using rhythmic initiation (hold relax and stretch reflex) for the rib cage. Training occurred for 50 minutes a day and three days per week for four weeks. Results: FVC, MPT, peak expiratory flow, and rib cage width were increased and decreased at the VAS point for rolling after treatment. Conclusion: Patients with cervical spinal cord injuries who had breathing problems felt uncomfortable when they had conversations on a couch. We found that PNF direct and indirect treatments improved rib cage width and breathing functions of patients with cervical spinal cord injuries.

6-단자를 이용한 직접 변환 수신 전 처리부 설계 (RF Front-end Design of Direct Conversion Receiver using Six-Port)

  • 장명신;김영완;고남영
    • 한국정보통신학회논문지
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    • 제9권7호
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    • pp.1534-1540
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    • 2005
  • 직접 변환 방식은 혼합기를 사용하는 구조와 6-단자 회로를 사용하는 구조를 갖는다. 6-단자 회로를 사용하는 방식은 혼합기를 이용하는 방식보다 회로 구조가 간단하고 집적화도 쉬운 장점을 가지고 있다. 그리고 다이오드 작동을 위한 전압이 혼합기를 작동시키기 위한 전압보다 낮은 장점을 가지고 있으며 LO에서의 소비전력이 낮고 광대역으로 구현하기 유리한 점을 보이고 있다. 본 논문에서는 branch-line 결합기로 구성되는 6-단자 회로와 ring hybrid 결합기로 구성되는 6-단자 회로를 설계하고 성능을 비교 분석하였다.

다양한 시험법에 의한 토목섬유 사이의 접촉 전단 강도 평가 (Evaluation of interface shear strength between geosynthetics using three kinds of testing methods)

  • 서민우;박준범;박인준
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2004년도 춘계학술발표회
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    • pp.356-363
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    • 2004
  • In this research, the shear behavior of four different interfaces consisting of 4 types of geosynthetics was examined, and both static and dynamic tests for the geosynthetic interface were conducted. The monotonic shear experiments were performed by using an inclined board apparatus and large direct shear device. The interface shear strength obtained from the inclined board tests were compared with those calculated from large direct shear tests. The comparison results indicated that direct shear tests are likely to overestimate the shear strength in low normal stress range where direct shear tests were not performed. Curved failure envelopes were also obtained for interface cases where two static shear tests were conducted. By comparing the friction angles measured from three tests, i.e. direct shear, inclined board, and shaking table tests, it was found that the friction angle might be different depending on the test method and normal stresses applied in this research. Therefore, it was concluded that the testing method should be determined carefully by considering the type of loads and the normal stress expected in the field.

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6-단자를 이용한 직접 변환 수신 전 처리부 설계 (RF Front-end Design of Direct Conversion Receiver using Six-Port)

  • 장명신;양우진;오대호;김영완;고남영
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2005년도 추계종합학술대회
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    • pp.481-484
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    • 2005
  • 직접 변환 방식은 혼합기를 사용하는 구조와 6-단자 회로를 사용하는 구조를 갖는다. 6-단자 회로를 사용하는 방식은 혼합기를 이용하는 방식보다 회로 구조가 간단하고 집적화도 쉬운 장점을 가지고 있다. 그리고 다이오드 작동을 위한 전압이 혼합기를 작동시키기 위한 전압보다 낮은 장점을 가지고 있으며 LO에서의 소비전력이 낮고 광대역으로 구현하기 유리한 점을 보이고 있다. 본 논문에서는 branch-line 결합기로 구성되는 6-단자 회로와 ring hybrid 결합기로 구성되는 6-단자 회로를 설계하고 성능을 비교분석하였다.

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A Modified Method for the Boundary Fitted Coordinate Systems to Analysis of Gas Bearings Considering Upstream In Extremely High Compressibility Number Region

  • Khan, Polina;Hwang, Pyung;Park, Sang-Shin
    • 한국윤활학회:학술대회논문집
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    • 한국윤활학회 2002년도 proceedings of the second asia international conference on tribology
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    • pp.105-106
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    • 2002
  • An expanded scheme of direct numerical solution method for solving the Reynolds' equation in the boundary fitted coordinate systems for the gas lubrication with ultra low clearance is presented. Skewed slider is calculated by this scheme and results are compared to the original direct numerical solution. The modified scheme has advantages in stability in high compressibility number region. At the lower A region the difference in results of original and modified method is several percents.

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리야푸노프 직접법에 의한 수중 글라이더의 깊이 제어 (Depth Control of Underwater Glider by Lyapunov's Direct Method)

  • 주문갑
    • 대한임베디드공학회논문지
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    • 제12권2호
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    • pp.105-112
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    • 2017
  • To control the depth of an underwater glider, a control method by using Lyapunov's direct method is proposed. The underwater glider has a torpedo-shape hull, a movable mass in the hull, and an inflatable buoyancy bag in the hull, but doesn't have large wings that increase the lift force for the conventional underwater glider. The control laws to adjust the position of the movable mass and the mass of the inflatable buoyancy bag are derived. For a selected speed and an angle of attack, we simulated the operation of the underwater glider using Matlab/Simulink. The efficiency of the proposed controller is shown in the fact that the control effort is active during only a short period of time when the zigzag trajectory is changed from downward to upward or vice versa.

직접 격자 사상법을 이용한 직사각컵 및 S-Rail 성형공정의 3차원 유한요소 역해석 (Three Dimensional Finite Element Inverse Analysis of Rectangular Cup and S-Rail Forming Processes using a Direct Mesh Mapping Method)

  • 김승호;허훈
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 2001년도 추계학술대회 논문집
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    • pp.81-84
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    • 2001
  • An inverse finite element approach is employed for more capability to design the optimum blank shape from the desired final shape with small amount of computation time and effort. In some drawing or stamping simulation with inverse method, it is difficult to apply inverse scheme due to the large aspect ratio or steep vertical angle of inclination. The reason is that initial guesses are hard to make out with present method for those cases. In this paper, a direct mesh marring scheme to generate initial guess on the sliding constraint surface described by finite element patches is suggested for one step inverse analysis to calculate initial blank shape. Radial type mapping is adopted for the simulation of rectangular cup drawing process with large aspect ratio and parallel type mapping for the simulation of S-Rail forming process with steep vertical angle of inclination.

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Development of Direct Surface Forming Process

  • Cho, Kwang-Hwan;Yoon, Kyung-Hwan
    • 한국소성가공학회:학술대회논문집
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    • 한국소성가공학회 2003년도 The Korea-Japan Plastics Processing Joint Seminar
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    • pp.73-77
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    • 2003
  • The backlight unit(BLU) is used as a light source of TFT liquid-crystalline-display (TFT-LCD) module. In this backlight unit, one of important components is the light guide, which is usually made of transparent polymers. Currently the screen-printing method is mainly used for the light guide as a manufacturing process. However, it has limitation to the flexibility of three-dimensional optical design. In the present paper a new alternative manufacturing method for the light guide with low-cost is proposed. This manufacturing method is named as direct surface forming (DSF), which is very similar to the well-known hot embossing except for partial contact between mold and substrate. The results of this new manufacturing method are presented in terms of processing condition, dimensional accuracy, productivity, etc.

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