• Title/Summary/Keyword: Dipping treatment

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Agrobacterium-Mediated Transformation of Phalaenopsis by Using Protocorm-Like Body (Protocorm-like body를 이용한 호접란 형질전환 연구)

  • Hur, Yeon-Jae;Kim, Eun-Young;Yang, Won-Tae;Lee, Young-Byoung;Lee, Jae-Hun;Jung, Young-Soo;Nam, Jae-Sung;Yun, Dae-Jin;Yi, Ki-Hwan;Kim, Doh-Hoon
    • Journal of Life Science
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    • v.19 no.3
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    • pp.378-383
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    • 2009
  • Agrobacterium tumefaciens-mediated transformation procedure for the phalaenopsis orchid, established by using Protocorm-like bodies (PLBs), was aimed at the introduction of target genes into individuals with divergent genetic backgrounds. PLBs obtained from the axillary bud of a peduncle were maintained on a hyponex medium supplemented with 1 g/l of activated charcoal, 30 g/l of sucrose and 0.1 mg/l thiamine. The multiplication rate of PLBs was about 90% in case of subculture PLBs to be cut transversely into 1/3 part from top position. The PLBs were inoculated with Agrobacterium strain EHA105 harboring both $\beta$-glucuronidase (GUS) and hygromycin-resistant genes for 20 minutes after dipping treatment. Transformation efficiency was the highest with a Agrobacterium culture medium and dipping treatment of O.D. 0.8. Newly induced PLBs were put on selection medium containing 1 mg/l hygromycin for 2 months. Hygromycin-resistant phalaenopsis plants that regenerated after the selection culture of PLBs showed histochemical blue staining due to GUS. Transgene integration of the hygromycin-resistant plants was confirmed by PCR and Southern blot using GUS specific primers and probe.

Bio-polishing and Silket Treatment of Cotton Yarns (면사의 효소가공 및 실켓 가공)

  • Bae, Young-Hwan;Lee, Ji-Wan;Son, Young-A;Kim, Ju-Hae;Kwon, Mi-Yeon;Kim, Eui-Hwa;Lee, Seung-Goo
    • Textile Coloration and Finishing
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    • v.20 no.4
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    • pp.15-20
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    • 2008
  • The cotton yarn was subjected to bio-polishing treatment with three commercial enzymes(Cellusoft L, Denimax-991L and Denimax-acid) to remove the fuzz on the cotton yarn. Also, enzyme treated cotton yarns were compared with singeing cotton yarns. Experimental variables of enzyme treated cotton yarn were as follow: concentration of enzyme solution and NaOH, dipping time, and processing temperature. The enzymatic treatments were evaluated by analyzing the effect on yarn count, twist contraction, evenness and tenacity. As the results, enzymatic treatment on cotton yarn induced same effects as the traditional singeing treatment. Also, silket treatment of cotton yarn after bio-polishing enhanced the tensile properties of the cotton yarn.

Effect of Nematicide-dipping Methods for the Control of Aphelenchoides fragariae in Strawberry (살선충제 침지처리에 의한 딸기잎선충 방제)

  • Kim, Dong-Geun;Kang, Myeong-Won;Lee, Joong-Hwan
    • Korean journal of applied entomology
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    • v.47 no.1
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    • pp.101-105
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    • 2008
  • Effects of different application of nematicides (fosthiazate 5% G, ethoprophos 5% G, and diazinon 34% EC) for the control of Aphelenchoides fragariae in strawberry were evaluated in a greenhouse experiments. Mother strawberry (Fragaria grandiflora) cv. Yeohong were dipped in solution of nematicides (fosthiazate or ethoprophos at 2.5 g a.i./liter in $20^{\circ}C\;or\;46^{\circ}C$) for 10 min. and planted in a greenhouse for dipping treatment. For the compare, mother strawberry were dipped in hot water for 10 min. without chemicals. For soil treatment, fosthiazate or ethoprophos at 3 kg a.i./ha were mixed into soil. For foliar spray, diazinon at 3.4 g a.i./liter was sprayed at foliage until runoff. At 40, 80, and 100 days after planting, runners were harvested from each treatment and the rate of nematode infestation and the number of nematodes per plant were examined. After 100 days of planting, mother strawberry plants dipped in fosthiazate solution (2.5 g a.i./liter, $20^{\circ}C$) for 10 min. produced more number of healthy runners and reduced % of infected runner as much as 90% and also had fewer nematodes per runner. Fosthiazate was more effective than ethoprophos. Foliar application of diazinon was reduced A. fragariae populations only in early season. Hot water treatment and nematicide soil treatment were less effective.

Effect of High Vanillin Treatment on Storage Quality of Fresh-cut Apples

  • Chung, Hun-Sik;Toivonen, Peter M.A.;Moon, Kwang-Deog
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.636-640
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    • 2009
  • The effect of post-cut vanillin treatment at high concentrations on changes of quality and microorganism in fresh-cut apples was studied. Apples (Malus domestica Borkh. cv. Fuji) were sliced, treated by dipping in different vanillin solution, 0, 40, 80, and 120 mM, packed in polyethylene bag, and then stored for up to 3 weeks at $4^{\circ}C$. Changes in total aerobic bacteria, yeast and molds, browning, soluble solids, and titratable acidity during storage were investigated. Growth of total aerobic bacteria throughout storage was strongly inhibited by vanillin regardless of treatment concentrations. Growth of yeast and molds was inhibited by vanillin of all concentrations until 2 weeks of storage. Levels of browning index, soluble solids, and titratable acidity were not significant difference among the treatment conditions until 2 weeks of storage. However, when stored for 3 weeks, browning index increased more at 80 or 120 mM vanillin, while soluble solids and titratable acidity more be decreased by 120 mM vanillin as compared with other treatment conditions. These results show that the usage of vanillin in processing of fresh-cut apples had a limitation for maintaining quality.

Patterning of high resolution metal electrodes using selective surface treatment and dip casting for printed electronics (선택적 표면처리와 딥코팅 방법을 이용한 고해상도 금속 패턴 형성연구)

  • Kim, Yong-Hoon;Eom, You-Hyun;Park, Sung-Kyu;Oh, Min-Seok;Kang, Jung-Won;Han, Jeong-In
    • Proceedings of the KIEE Conference
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    • 2009.07a
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    • pp.1340_1341
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    • 2009
  • In this report, high-resolution metal electrode patterning is demonstrated by using selective surface treatment and dip casting for low-cost printed electronic applications. On hydrophobic octadecyltrichlorosilane treated $SiO_2$ surface, deep UV irradiation was performed through a patterned quartz photomask to selectively control the surface energy of the $SiO_2$ layer. The deep UV irradiated region becomes hydrophilic and by dipping into Ag nano-ink, Ag patterns were formed on the surface. Using this patterning technique, line patterns and dot arrays having less than $10{\mu}m$ pitch were fabricated.

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Preparation and Characterization of Antireflective Film in $TiO_2-SiO_2$ System by Sol-Gel Method (Sol-Gel법에 의한 $TiO_2-SiO_2$계 저반사 박막의 제조 및 특성)

  • 윤태일;최세영;이용근;이재호
    • Journal of the Korean Ceramic Society
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    • v.30 no.9
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    • pp.775-783
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    • 1993
  • TiO2-SiO2 system anti-reflective(AR) film was prepared to decrease reflectance on the glass surface. The experiments were carried out as fellow, 1) preparation & hydrolysis of TiO2-SiO2 system sols. 2) glass dipping, and 3) drying & heat treatment. We investigated the refractive index and thickness of film with viscosity, zeta-potential of sol, sol concentration, withdrawal speed, drying and heat treatment condition. As a result, we prepared good qualitative Quarter-Half-Quarter type anti-reflective film that had minimum, 0.02% and average reflectance, 0.087% in the visible region.

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Grapefruit Seed Extract (DF-100) Treatment of Poultry to Reduce Attached Salmonella

  • Mike F. Slavik;Kim, Jeong-Weon;Phil L. Matsler;Hong Wang
    • Journal of Food Hygiene and Safety
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    • v.11 no.1
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    • pp.7-10
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    • 1996
  • Chicken skins or carcasses inoculated with Salmonella typhimurium were exposed to 0.1 or 0.5% grapefruit seed extracts (DF-100) for 1 or 3 min to evaluate antibacterial activity of DF-100 and its possible application in proultry processing. The numbers of live salmonellae on chicken skins were reduced by 0.8-1.2 logs/cm2 with 0.5% DF-100. Dipping chicken carcasses into 0.5% DF-100 for 3 min reduced salmonelae by 4.3 logs/carcass. Scanning electron microscopy showed that DF-100 killed the cells attached but did not detach cells from the skin. No odor or changes in the color of chicken skin were detected after DF-100 treatment.

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오갈피 나무속(Acanthopanax spry.) 식물의 번식에 관한 연구 -제1보 삽목시 생장조절성질이 발근에 미치는 영향-

  • 안상득;최은옥
    • Journal of Ginseng Research
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    • v.16 no.2
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    • pp.138-145
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    • 1992
  • Rooting test of 5 species plants in Acanthopanax genus was carried out to see the comparative effect of growth regulators NAA IBA and IAA. And the results are summarized as in the following compendia; (1) When hard and soft-wood cutting from new shoot of 5 species were taken and dipping treated with growth regulators such as NAA IBA and IAA at varied concentration levels their roofings were mostly more superior to the control that untreated. And also treatment of NAA and IBA among treated substances were more effective than IAA. (2) The effects on the rooting number and rate made a great difference according to species of Acanthopanax genus even if the same regulator was treated. (3) Decreasing effects of rooting by high concentration obstacle (especially at 2,000 rpm) of NAA and IBA in other species except A. sieboldianum were mostly serious. On the other hand, that in case of IAA treatment was not risen in most species except A. sessiliflorus.

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The Effect of the Binder to Zeolite Thin Film Coating by Heat Treatment (열처리를 통한 제올라이트 박막 코팅 시 바인더의 영향)

  • Yoo, Young-Seok;Jo, Jun-Ho;Kim, I-Tae
    • Journal of the Korean institute of surface engineering
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    • v.46 no.6
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    • pp.277-282
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    • 2013
  • This study is an experimental attempt to confirm the binder effect of zeolite coating on glass plate by heat treatment. As a result, zeolite was successfully formed with low concentratios of pressure, whose concentration was effective in 10% or more for thin film zeolite coating. And as the content of the binder (TEOS) in mixed coating solution was higher, the zeolite was fastened better on the surface. Above 5% content of the binder in the coating solution, TEOS hindered zeolite synthesis of the precursor and brought to zeolite capacity decrease. Furthermore, when the concentration of the precursor, sedimentation rate of the precursor was higher and the coating efficiency is reduced thereby. Therefore, the most effective concentrations of the precursor and TEOS in the coating solution was 10% and 5%, respectively. It was concluded that zeolite coating is produced by heat treatment method after dipping without hydrothermal synthesis.

Effects of combined argon gas treatment on the quality of fresh-cut potatoes (Argon gas 병용처리가 신선편이 감자의 품질에 미치는 영향)

  • Son, Hyun-Ju;Moon, Kwang-Deog
    • Food Science and Preservation
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    • v.21 no.2
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    • pp.163-169
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    • 2014
  • The effect of the combined argon (Ar) gas packaging treatment on the browning of fresh-cut potatoes was studied. Fresh-cut potatoes were prepared for the following six groups: dipping distilled water for 1 minute and air packaging (Cont); dipping in distilled water for 1 minute and argon gas packaging (AR); dipping in 1% ascorbic acid for 1 minute and air packaging (AA); dipping in 1% ascorbic acid for 1 minute and argon gas packaging (AAR); blanching at $35^{\circ}C$ for 30 minutes and air packaging (BL); and blanching at $35^{\circ}C$ for 30 minutes and argon gas packaging (BAR). The potatoes were washed, peeled, and sliced ($1.5{\times}1.5{\times}1.5$ cm) before treatment. The samples were packed with a 0.04-mm-thick OPP film and were stored at $5^{\circ}C$ for 9 days. During the storage, the $O_2$ concentration decreased in Cont but increased in the AR, AA, AAR, BL, and BAR groups. The $CO_2$ concentration increased during storage. The AR, AAR, and BAR groups showed high $L^*$ and low $a^*$, $b^*$ values (browning index). The growth of the total aerobic bacteria was also inhibited in the AR group. During storage, the PPO activity gradually increased, and the AR group showed lower PPO activity. The AA and AAR groups showed high DPPH radical scavenging activity. It was demonstrated that the argon gas packaging is effective in the quality maintenance of fresh-cut potatoes.