• 한국축산식품학회지
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• 제30권5호
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• pp.773-779
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• 2010

The present study was designed to determine the physicochemical properties of yam (Dioscorea) solution fermented by L. bulgaricus, the protective effect of fermented Dioscorea opposita Thunb. on stomach lesions, and the possibility of its application to fermented dairy products. When the concentration of yam increased, the pH increased in raw powder samples but not in extracted powder samples. In 6% raw powder containing the sample, the viable cell counts showed a dramatic increase, whereas those in extracted yam powder significantly decreased in all concentrations during 32 h of fermentation. The viscosity of extracted yam powder showed a lower value than that of raw powder, which was maintained throughout the fermentation. The solution of Dioscorea opposita Thunb. raw powder showed a significantly higher allantoin content compared with the extracted powder (p<0.05). At 2% of added Dioscorea opposita Thunb., 0.65 mg/mL was contained in both samples; however, when 6% of Dioscorea opposita Thunb. was added, the amount of diosgenin increased up to 1.9 mg/mL. When 200 mg of powder/kg body wt. was injected, gastric lesions were apparently reduced compared with those of control rats. With 100 mg/kg body wt. injected into rats, the protective effect was a little lower than that in 200 mg/kg body wt. Based on these data, the present study indicated that yam powder fermented by L. bulgaricus showed a protective effect on gastric lesions in rats; therefore, it may be included as an added ingredient in yogurt manufacture.

• Journal of Dairy Science and Biotechnology
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• 제30권2호
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• pp.69-74
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• 2012

This study was performed to determine by the ability of the mixed culture of Lactobacillus bulgaricus and Streptococcus thermophilus to ferment Dioscorea opposita Thunb. (yam) and to evaluate the nutraceutical value of fermented yam. The titratable acidity (TA) value increased from 2 to 6% with increased concentrations in both raw yam and extracted lactic acid bacteria (LAB) fermented yam (LFY). The viable cell counts and the allantoin and diosgenin contents were higher in raw LFY at large concentrations (6%) than in extracted LFY samples at all fermentation periods up to 32 h. Based on these data, it confirmed that raw yam fermented by the combination culture of L. bulgaricus and S. thermophilus for various fermentation periods favors the symbiotic growth of LAB and results in higher nutraceutical content.

• 한국작물학회지
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• 제47권4호
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• pp.297-300
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• 2002

Endogenous ABA and batatasins were first quantified in the stored tubers and bulbils of the Chinese yam, Dioscorea opposita Thunb. cv. 'Tsukune' by GC-MS with comparison of internal standard, and sugar content was also analyzed by HPLC with comparison of external standard. Endogenous ABA content in stored tubers and bulbils was rapidly decreased as storage period prolonged. ABA content of the bulbils was always higher than that of tuber during storage periods. All batatasins of stored tubers and bulbils at 30 days after storage decreased steadily compared to 120 days after storage. On the contrary, batatasin-Ⅲ of the stored tuber and bulbils was only decreased at 60 days after storage. In Dioscorea opposita Thunb. cv. 'Tsukune' like D. japonica, D. alata, and D. bulbifera, may be controlled by endogenous batatasins and ABA, In these compounds, batatasins rather than ABA might be closely related to dormancy-inducing compound during dormancy of the Chinese yam, Dioscorea opposita Thunb. cv. Tsukune.

• 한국작물학회지
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• 제56권3호
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• pp.250-254
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• 2011

The study was carried out to evaluate the affect of proton beam radiation on production of bulbil and tuber including change of endogenous gibberellins, of Dioscorea opposita Thunb. The yield of bulbils and tubers from non- and irradiated D. opposita Thunb at doses of 5, 10, 15 and 20 Gy were determined. Endogenous gibberellins were also quantified by GC/MS analysis. D. opposita tubers irradiated at 15 Gy produced higher bulbil production than non-irradiated plants. Enlarged bulbil (above size diameter 4 mm) was significantly increased at 15 Gy. Bioactive endogenous $GA_4$ was dominant in bulbils and tubers irradiated with proton beam rather than $GA_1$. Major gibberellins biosynthetic pathways in bulbils and tubers of D. opposita plants were non C-13 hydroxylation route. From the results of this study, 15 Gy proton beam radiation was suggested as an optimal dose that can produce high amounts of bulbil for mass production of D. opposita plant.

• 한국약용작물학회지
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• 제7권3호
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• pp.167-171
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• 1999

마 재배에 있어서 트랜쳐를 이용한 심경재배 효과와 트렌치 깊이에 따른괴경의 형태적 특성 및 수량성의 변화를 자주식 보행형 트렌쳐인 "NF-827H"를 공시하여 검토한 결과를 요약하면 다음과 같다. 트렌처를 이용한 심경으로 마를 재배하면 관행 경운에 비하여 괴경의 크기가 커지면서 모양이 좋아지고 상품성이 높은 괴경의 수량이 크게 증가되었다 트렌치에 의한 심경재배 효과는 단마보다 장마에서 크게 나타났으며, 괴경의 크기와 모양 및 수량성을 고려할 때 단마는 60cm 이상, 장마는 80cm 이상으로 트렌치하는 것이 좋다.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.151.1-151
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• 2003

Chinese yam(D. opposita Thunb. cv. Jang-Ma) plants showing necrotic mosaic symptom were collected from their growing fields in Andong, Korea. Electron microscoptic examination of negatively stained preparations showed filamentous particles of 660nm in length. The virues purified partially were used to isolate Viral RNA as template for RT-PCR to amplify the CP gene with ChYNMV specific and oligo dT primers. Amino acids sequeces revealed that the viruses shared 99.3％ similarity with ChYNMV(AB044386) which was known as the member of macluravirus. So the viruses from Chinese yam(D. opposita Thunb. cv. Jang-Ma) plants were identified as ChYNMV. Comparing the amino aced sequences of ChYNMV strains with other macluraviruses such as CdMV, NLV and MacMV revealed that N-terminal was the most variable region and conserved regions were present within the genus Macluravirus.

• 한국작물학회지
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• 제54권2호
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• pp.198-202
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• 2009

The starch properties of two chinese yams were evaluated in this study. Amylose content ranged 13.5% to 19.3%. The swelling power of starches varied 10.98% to 11.43%. Water binding capacity in chinese yam starches ranged 93.46% to 107.21%, high WBC was observed in Ma 1. The onset temperature (To) of two chinese yam starches ranged 62.9 to $75.0^{\circ}C$, peak temperature (Tp) ranged 76.2 to $84.7^{\circ}C$. The PHI(peak height index) was about 4-fold higher in Anwon cultivar than in Ma 1 cultivar. SEM revealed that starches has a presence of large oval or spherical to small irregular-shape granules. Starch granule size ranged 15.23 to 15.52 ${\mu}m$, showing a typical C-type X-ray pattern.

• 한국약용작물학회지
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• 제8권2호
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• pp.89-93
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• 2000

절편씨마를 이용하여 마를 재배할 경우 토성에 따른 괴경의 형태적 특성과 수량성의 차이를 단마 품종 '마1호'와 장마 품종 '안동재래종'을 공시하여 검토한 결과를 요약하면 다음과 같다. 사토에서는 괴경의 길이가 짧고 분기의 발생이 많아 괴경의 모양이 좋지 않았다. 양토와 사양토에서는 사토에 비하여 분기 발생이 적어 괴경의 모양이 양호하였으며, 괴경의 생체수량은 단마가 $21{\sim}27%$, 장마가 각 6% 증수되었고 , 상마수량은 단마가 $45{\sim}55%$, 장마가 $20{\sim}22%$ 증수되었다. 식양토에서 단마는 괴경의 분지 발생이 다소 많았으며, 장마는 괴경의 굵기가 가늘고 만곡되어 모양이 좋지 않았다.

• 한국약용작물학회지
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• 제20권3호
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• pp.190-194
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• 2012

Shoot tips of chinese yam (Dioscorea opposita Thunb.) were cultured on MS medium containing 0.5 mg/L BA to produce micro-tubers in vitro. To stimulate the formation of shoots and micro-tubers, and produce large micro-tubers, the sections of micro-tubers were cultured on MS media with BA and IAA. The shoot multiplication, and the micro-tuber formation and growth were very effective on the media containing 2.0 mg/L BA and 0.5~1.0 mg/L IAA. Sucrose added to MS medium with 2.0 mg/L BA and 0.5 mg/L IAA to stimulate more micro-tuber growth. The medium added 50 g/L sucrose was very effective in the increase of plant fresh weight and micro-tuber growth. After 4 weeks' culture of micro-tuber sections on the medium with 2.0 mg/L BA, 0.5 mg/L IAA and 50 g/L sucrose, the liquid media were added into the same vessels. The micro-tuber growth was stimulated remarkably by the addition of liquid medium. The addition of 25 $m{\ell}$ liquid medium containing 10 g/L activated charcoal, 3x MS salts and 250 g/L sucrose was the most effective in micro-tuber growth.

• 한국자원식물학회지
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• 제24권2호
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• pp.208-213
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• 2011

둥근마 기내 식물체의 증식을 위해서는 고체배지보다 액체배지에서 액아수, 초장, 생체중, 괴경형성의 수가 높아 기내식물체 증식에 효과적이었다. 배지의 무기염류 농도는 MS 기본 염류의 배지에서 액아수와 생체중이 각각 28.8개, 3.6 g로 기본 염류의 양을 반으로 줄이거나 두 배로 늘인 배지에서보다 높았다. 당원으로는 sucrose가 glucose 보다 더 효과적이었고, sucrose의 농도는 60 $g{\cdot}L^{-1}$에서 액아수와 괴경형성수가 가장 높아 효율적이었다. 질소원은 $NH_4NO_3$와 $KNO_3$의 조성 비율이 $1{\times}{\frac{1}{2}}$인 것이 다른 처리에 비해 생육이 우수한 것으로 나타났다. 배지내 수소이온 농도는 pH 4.8 ~ 6.8 범위내에서 pH간에 뚜렷한 유의 차이를 보이지 않았다. 생장시기별 생장량에 있어서 엽수, 마디수 및 초장은 접종후 2주부터 5주까지 급격히 증가하고 12주까지는 서서히 증가하는 유사한 경향을 보였다. 반면, 생체중은 접종 3주에서 11주까지 꾸준하게 증가하여 최고치에 달했다. 괴경은 접종 2주부터 형성되기 시작하여, 5~8주 사이에 높은 증가율을 가지며 11주에 최고 형성수가 되었다.