• 대한수의학회지
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• 제36권1호
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• pp.151-159
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• 1996

This study was carried out to evaluate the effects of all-trans retinoic acid(RA) on the development of cholangiocarcinoma in hamsters. Eighty six female Syrian golden hamsters were divided into four groups. Group I was for the induction of the cholangiocarcinoma, which was infected orally with C sinensis and given dimethylnitrosamine(DMN, 15ppm) in drinking water for 4 weeks. Group II was for evaluating the effect of all-trans RA treatment on the cholangiocarcinogenesis, which was treated the same as group I and orally given RA(1mg/kg, 5 times per week) for 15 weeks. Group III was given only RA hr 15 weeks. Control group IV was given only soybean oil which was solvent for RA treatment. More than 5 heads of hamsters in each group were sacrificed at 4, 7, 11 and 15 weeks after the begining of the experiment. The livers were examined grossly, histopathologically, and immunohistochemically. The results obtained were as follows : 1. Death of animals started from the 11 weeks after the begining of the experiment. One of the total 22 animals(5%) and 7 of the total 24 animals(29%) died in group I and group II, respectively. 2. Proliferation of oval cell was peaked at 11 weeks in group I and at 7 weeks in group II, and decreased gradually after those periods of the time. 3. Cholangiocarcinomas were found in 1 of 6 animals(17%) at 11 weeks and in 4 of 6 animals(67%) at 15 weeks in group I, respectively. But in group II, the cholangiocarcinomas occured in 1 of 5 animals(20%) at 7 weeks, in 7 of 12 animals(58%) at 11 weeks and in 2 of the rest animals(100%) at 15 weeks, respectively. 4. Expression of $\alpha$-fetoprotein(AFP) of the oval cells in the group II showed the same degree of positive reaction at that of group I at 4 weeks. But AFP postive oval cells decreased gradually and AFP negative oval cells(ductlike oval cells) increased gradually. 5. Expression of cytokeratin of the oval cells in group II was shown slightly at 4 weeks and the degree of expression increased moderately from the 7 weeks. But the expression of the oval cells in group I was shown slightly after the 7 weeks. These results suggested that all-trans RA promoted the occurrence and the rate of cholangiocarcinoma by inducing differentiation of small cells and oval cells in the liver of hamsters infected with C sinensis and treated with DMN.

• 한국식품과학회지
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• 제11권3호
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• pp.147-152
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• 1979

한국(韓國) 식품(食品)과 인(人) 타액중(唾液中)의 질산염(窒酸鹽) 및 아질산염(亞窒酸鹽)의 함량(含量) 관계(關係)에 관(關)한 연구(硏究)를 목적(目的)으로 한국(韓國) 식품(食品)과 섭취(攝取)한 식품(食品) 중(中)의 질산염(窒酸鹽)과 타액중(唾液中)의 질산염(窒酸鹽) 및 아질산염(亞窒酸鹽)과의 함량(含量)과의 관련성(關聯性)을 구명(究明)한 결과(結果) 다음과 같은 결론(結論)을 얻었다. 1) 한국(韓國) 식품중(食品中)의 질산염(窒酸鹽)의 표준(標準) 분석치(分析値)는 배추가 2,500, 시금치가 2,000, 무청과 무에도 역시 시금치와 같이 2,000, 캬베쓰의 경우 500, 햄 및 쏘시지와 오이도 300이고 당근도 마찬가지로 300이었으며 콩은 200, 가지는 150, 도마도는 80, 고구마가 80 및 양파가 70 ppm이었다. 2) 조사(調査) 대상(對象) 모두 150명(名)의 아침 식사(食事)의 menu의 식품중(食品中)의 질산염(窒酸鹽) 및 아질산염(亞窒酸鹽)의 양(量)은 식품(食品)에 함유(含有)되는 질산염(窒酸鹽)의 표준(標準) 분석치(分析値)에 기준(基準)하였다. 그 결과(結果)로 한국인(韓國人)의 타액중(唾液中)의 질산염(窒酸鹽) 및 아질산염(亞窒酸鹽)의 함량(含量)은 미국인(美國人)의 타액(唾液)에서의 분석치(分析値) 보다는 훨씬 높고 또한 비교적(比較的) 우리나라의 식생활(食生活)과 비슷한 일본인(日本人)의 타액(唾液)보다도 더욱 높은 수치(數値)를 나타냈으며 이는 우리의 식생활(食生活)과 관련성(關聯性)이 있다.