• Korean Journal of Weed Science
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• v.6 no.2
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• pp.134-145
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• 1986

The experiment was crried out to evaluated the herbicidal properties of bensulfuron methyl [methyl 2-[[[[[(4, 6-dimethoxy pyrimidine-2yl) amino] carbonyl] amino] sulfonyl] methyl] benzoate]. No phytotoxicity was observed when bensulfuron methyl was applied at 3 and 6 g a.i./ 10a while the application rate 12 g a.i./10a slightly retared the growth of rice. The phytotoxicity decreased as the application time was delayed. The effect of application rate, leaching grade, transplanting depth, soil type and temperature on crop injury was little. Japonica variety (Dong-Jin) was more sensitive to bensulfuron methyl than indica X japonica variety (Sam-Kang). Bensulfuron methyl controlled effectively perennial weeds such as Sagittaria pygmaea Miq., Potamogeton diatinctus A. Benn., Cyperus serotinus Rottb., Sagittaria trifolia L., Eleocharis kuroguwai Ohwi. including most annual weeds except Echinochloa crus galli P. Beauv. The effect slightly decreased with lowering the temperature increasing the leaching grade. Application time and soil type employed did not affect the weeding effect.

• Korean Journal of Weed Science
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• v.14 no.3
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• pp.171-175
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• 1994

Physiological changes in Eleocharis kuroguwai Ohwi as affected by bensulfuron-methyl {Methyl 2-[[[[[(4, 6-dimethoxy-2-pyrimidinyl)amino]carbonyl]amino] sulfonyl]methyl]benzoate} was determined to relate the characteristics with regrowth behavior. There were no changes in relative growth rate(RGR) during the period of growth cessation after application of bensulfuron-methyl. RGR's of the growth ceseased plants caused at 39 and 51 g/ha began to increase in between 25 and 30 days after application (DAA) and between 30 and 35 DAA, respectively. In untreated plant tuber carbohydrate rapidly decreased right after emergence and almost consumed within 40 days. There was no carbohydrate consumption during the period of growth cessation in bensulfuron-methyl-applied plant, but the content started to rapidly decrease with regrowth. Tuber viability lasted for 30 days in untreated plant, while tubers were viable for 60 and 70 days after application of bensulfuron-methyl at 39 and 51 g/ha, respectively. During the period of growth cessation the plants kept minimum respiration and photosynthesis, but with regrowth respiration and photosynthesis were resumed and rapidly increased.

• Journal of the Korean Electrochemical Society
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• v.19 no.2
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• pp.29-38
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• 2016

Screen printed carbon electrodes (SPCEs) with immobilized osmium-based hydrogel redox polymer, uricase and PEGDGE can be used to apply uric acid electrochemical detecting. The osmium redox complexes were synthesized by the coordinating pyridine group having different functional group at 4-position with osmium compounds. The synthesized poly-osmium hydrogel complexes are described as PAA-PVI-$[Os(dCl-bpy)_2Cl]^{+/2+}$, PAA-PVI-$[Os(dme-bpy)_2Cl]^{+/2+}$, PAA-PVI-$[Os(dmo-bpy)_2Cl]^{+/2+}$. The different concentrations of uric acid were measured by cyclic voltammetry technique using enzyme-immobilized SPCEs. The prepared SPCEs using PAA-PVI-$[Os(dme-bpy)_2Cl]^{+/2+}$ showed no interference from common physiologic interferents such as ascorbic acid (AA) or glucose. The resulting electrical currents at 0.33 V vs. Ag/AgCl displayed a good linear response with uric acid concentrations from 1.0 to 5.0 mM. Therefore, this approach allowed the development of a simple, point of care in the medical field, disposable electrochemical uric acid biosensor.

• Korean Journal of Weed Science
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• v.7 no.2
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• pp.165-170
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• 1987

This study was undertaken to elucidate the behavior of herbicide bensulfuron-methyl[methyl-2-[[[[[(4,6-dimethoxy pyrimidine-2yl)amino]carbonyl]amino]sulfonyl]methyl]benzoate]in soils. Adsorption of the herbicide in soils was mainly correlated with content of organic matter and clay, and canon exchange capacity. Adsorption distribution coefficient(Kd value) in clay loam soil was greater than those in loam and sandy loam soils. The Kd value decreased in the order of zeolite, bentonite, halloysite and laziolite clay minerals. Bensulfuron-methyl moved to 3cm deep in clay loam soil and 4cm deep in sandy loam and herbicide treated layer was 0 to 2cm profile in the two soils. The decomposition rate of bensulfuron methyl varied with the soil properties. The rate was slower in sterilized soil than in nonsterilized. Addition of organic matters to the soils accelerated the decomposotion. The degradation was more rapid in 30$^{\circ}C$ soil temperature than in 20$^{\circ}C$.

• Korean Journal of Weed Science
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• v.13 no.1
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• pp.55-61
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• 1993

Difference in sensitivity of Eleocharis kuroguwai tubers to bensulfuron- methyl{methyl 2-[[[[[(4, 6-dimethoxy-2-pyrimidinyl) amino] carbonyl] amino] sulfonyl] methyl] benzoate} at different burial depths were determined with respect to tuber emergence, sprouting of lateral buds, carbohydrate consumption of the tuber, and growth and new tuber production of the regrown plants. Days required to regrowth from the growth cessation due to bensulfuron-methyl were shorter in shallow-buried tubers than in deep-buried tubers. With application of bensulfuron-methyl shallow-buried tubers consumed less carbohydrate in the tuber than deep-buried tubers as compared with in deep-buried tubers during the period of growth cessation and greater regrowth also occurred in the former. Fast and great regrowth in shallow-buried tubers resulted in great production of dry matter and new tubers. However, the differences obtained were not due to bensulfuron-methyl, but due mainly to ecological emergence and growth characteristics of tubers buried at different depths.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.5
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• pp.839-846
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• 2004

The purpose of this study was to investigate the possibility of medicinal plants application as an edible functional food resource. This research was planned to apply Artesmisia capillaris, which is one of the most widely used oriental medicinal resource into beverages, and to evaluate the validity and the nutrients of the developed beverage with Artemisia capillaris. Following this plan, we analyzed contents of the general nutritional composition, mineral and amino acid contents. And to evaluate the improvement of blood circulation and the ability to recover from fatigue, we had a group of athletes to regularly take the beverage before and after exercise and checked the heart rates, and blood components before exercise and after exercise. Brix, pH and titratable acidity of develoued drink were 9.2%, 4.4 and 0.04%, respectively. The approximate nutritional composition of beverage was carbohydrate, 2.70%, crude protein, 6.00%, crude ash, 0.20%, and crude fat, 0.10%. And developed beverage contained essential amono acids and minerals (p, Ca, K, Na, Fe, Mn, Zn). Heart rates, lactic acid, glucose, creatinine, LDH, GOT, GPT and lipid concentratios in blood were decreased after taking the beverage with Artemisia capillaris for 6 weeks, both in stable condition and after exercise. Since the athletes who participated In our research were doing aerobic exercise regularly, we judged that this result was formed not only by aerobic exercise, but also by the function of developed beverage with Artemisia capillaris the athletes took for 6 weeks. We believe that taking the beverage regularly will help the improvement of the public health and the athleticism of the athletes. We hope that this result will be used as the basic resource in developing of the beverage with Artemisia capillaris in the near future.

• Asian Pacific Journal of Cancer Prevention
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• v.16 no.16
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• pp.7179-7188
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• 2015

Cancer is a major health obstacle around the world, with hepatocellular carcinoma (HCC) and colorectal cancer (CRC) as major causes of morbidity and mortality. Nowadays, there isgrowing interest in the therapeutic use of natural products for HCC and CRC, owing to the anticancer activity of their bioactive constituents. Boswellia serrata oleo gum resin has long been used in Ayurvedic and traditional Chinese medicine to alleviate a variety of health problems such as inflammatory and arthritic diseases. The current study aimed to identify and explore the in vitro anticancer effect of B. Serrata bioactive constituents on HepG2 and HCT 116 cell lines. Phytochemical analysis of volatile oils of B. Serrata oleo gum resin was carried out using gas chromatography-mass spectrometry (GC/MS). Oleo-gum-resin of B. Serrata was then successively extracted with petroleum ether (extract 1) and methanol (extract 2). Gas-liquid chromatography (GLC) analysis of the lipoidal matter was also performed. In addition, a methanol extract of B. Serrata oleo gum resin was phytochemically studied using column chromatography (CC) and thin layer chromatography (TLC) to obtain four fractions (I, II, III and IV). Sephadex columns were used to isolate ${\beta}$-boswellic acid and identification of the pure compound was done using UV, mass spectra, $^1H$ NMR and $^{13}C$ NMR analysis. Total extracts, fractions and volatile oils of B. Serrata oleo-gum resin were subsequently applied to HCC cells (HepG2 cell line) and CRC cells (HCT 116 cell line) to assess their cytotoxic effects. GLC analysis of the lipoidal matter resulted in identification of tricosane (75.32%) as a major compound with the presence of cholesterol, stigmasterol and ${\beta}$-sitosterol. Twenty two fatty acids were identified of which saturated fatty acids represented 25.6% and unsaturated fatty acids 74.4% of the total saponifiable fraction. GC/MS analysis of three chromatographic fractions (I,II and III) of B. Serrata oleo gum resin revealed the presence of pent-2-ene-1,4-dione, 2-methyl- levulinic acid methyl ester, 3,5- dimethyl- 1-hexane, methyl-1-methylpentadecanoate, 1,1- dimethoxy cyclohexane, 1-methoxy-4-(1-propenyl)benzene and 17a-hydroxy-17a-cyano, preg-4-en-3-one. GC/MS analysis of volatile oils of B. Serrata oleo gum resin revealed the presence of sabinene (19.11%), terpinen-4-ol (14.64%) and terpinyl acetate (13.01%) as major constituents. The anti-cancer effect of two extracts (1 and 2) and four fractions (I, II, III and IV) as well as volatile oils of B. Serrata oleo gum resin on HepG2 and HCT 116 cell lines was investigated using SRB assay. Regarding HepG2 cell line, extracts 1 and 2 elicited the most pronounced cytotoxic activity with $IC_{50}$ values equal 1.58 and $5.82{\mu}g/mL$ at 48 h, respectively which were comparable to doxorubicin with an $IC_{50}$ equal $4.68{\mu}g/mL$ at 48 h. With respect to HCT 116 cells, extracts 1 and 2 exhibited the most obvious cytotoxic effect; with $IC_{50}$ values equal 0.12 and $6.59{\mu}g/mL$ at 48 h, respectively which were comparable to 5-fluorouracil with an $IC_{50}$ equal $3.43{\mu}g/mL$ at 48 h. In conclusion, total extracts, fractions and volatile oils of B. Serrata oleo gum resin proved their usefulness as cytotoxic mediators against HepG2 and HCT 116 cell lines with different potentiality (extracts > fractions > volatile oil). In the two studied cell lines the cytotoxic acivity of each of extract 1 and 2 was comparable to doxorubicin and 5-fluorouracil, respectively. Extensive in vivo research is warranted to explore the precise molecular mechanisms of these bioactive natural products in cytotoxicity against HCC and CRC cells.

• Applied Biological Chemistry
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• v.39 no.1
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• pp.70-76
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• 1996

A series of the herbicidal pyridylsulfonyl areas, none substitutent, 1-(4,6-dimethoxypyrimidine-2-yl)-3-(2-pyridylsulfonyl) urea, 3 and 3-trifluoromethyl substitutent, 1-(4,6-dimethoxypyrimidine-2-yl)-3-(3-trifluoromethyl-2-pyridylsulfonyl) urea, 5(Flazasulfuron) were synthesizied and the rate of hydrolysis of their has been studied in 25%(v/v) aqueous dioxane at $45^{\circ}C$. From the results of solvent effect($m{\ll}1,\;n{\ll}3\;&\;{\mid}m{\mid}{\ll}{\mid}{\ell}{\mid}$), thermodynamic parameter (${\Delta}S^{\neq}=0.54{\sim}\;-2.19\;e.u.\;&\;{\Delta}H^{\neq}=0.025\;Kcal.mol.^{-1}$), hydrolysis product analysis, $pK_a$ constant(3: 4.9 & 5: lit.4.6) and the rate equation, a marked difference in the kinetics of the reaction of 3 and 5(Flazasulfuron) was observed. It may be concluded that the hydrolysis of 5 proceeds through the $A-S_N2Ar$ reaction via conjugate acid$(5H^+)$ below pH 7.0, whereas, above pH 9.0, the hydrolysis proceeds through irreversibly $(E_1)_{anion}$ and reversibly $(E_1CB)_R$ mechanism via conjugate base(CB), respectively. But in case of 5, $A-S_N2Ar,\;(E_1)anion\;and\;(E_1CB)_R$ mechanism involved Smile rearrangement. The mate of rearrangement of 5 to a 3-trifluoromethyl-2-pyridylpyrimidinyl urea(PPU) in acid and 3-trifluoromethyl-2-pyridyl-4.6-dimethoxypyridinyl amine (PPA) in base was increased about 3.5 times by the introduction of trifluoromethyl group in the 3-position on the 2-pyridyl ring. From the basis of these findings, a possible mechanism for the hydrolysis of 5 was proposed and discussed.

• Korean Journal of Weed Science
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• v.12 no.2
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• pp.110-123
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• 1992

To investigate the safening effect of dymron against cinosulfuron, the responses of 104 rice cultivars and lines to single and mixture treatments of cinosulfuron [3-(4.6-dimethoxy-1,3,5-triazin-2-yl)-1-[2-(2-methoxyethoxy)-phenylsulfonyl]-urea] and dymron [1-(${\alpha}$, ${\alpha}$-dimethylbenzyl)-3-p-tolyl urea] were evaluated. The responses of 104 rice cultivars and lines to cinosulfuron were differed. Among the rice cultivars, growth of Calrose, Dasukei, Hwajinhyeo, and Milyang 105 was significantly inhibited by cinosulfuron but there was no inhibition in Daeseongbyeo, Iri 371, Jangseongbyeo, Taebaegbyeo and IR 74. The japonica types were generally inhibited more than the indica and indica${\times}$japonica types. When Kwangmyungbyeo was treated with the mixtures of cinosulfuron 48g and 96g ai/ha with dymron 450g ai/ha, the inhibition of plant height was significantly reduced regardless of dymron concentrations. With increase in cinosulfuron concentration, the safening effect of dymron against cinosulfuron was more evident in plant height than fresh weight. On the other hand, dymron did not have any safening effects of cinosulfuron on Echinochloa crus-galli at any concentrations. The mixtures of dymron and cinosulfuron produced antagonistic effects on the plant height regardless of rice cultivars and treatment concentrations. At cinosulfuron 24g ai/ha, the effects of the mixtures of cinosulfuron and brassinolide on the plant height of Kwangmyungbyeo and IR 74 were similar irrespective of brassinolide concentrations but the inhibition of Kwangmyungbyeo was greater than that of IR 74 as the concentrations of cinosulfuron increased. The effects of the mixtures of cinosulfuron at concentration of 96g ai/ha with various ABA levels on the plant height of both Kwangmyungbyeo and IR 74 showed synergistic effect regardless of ABA concentration. However, the response of fresh weight of both cultivars treated with cinosulfuron at 96g ai/ha with ABA synergisism was observed in Kwangyungbyeo while IR 74 showed antagonism.