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First detection of avian bornavirus by RT-PCR in proventricular dilatation disease-suspected Hahns Macaw (Ara nobilis nobilis) in Korea (선위확장증-의심 한스 마코앵무새(Ara nobilis nobilis )로부터 RT-PCR에 의한 avian bornavirus 최초 검출)

  • Kim, Jin-Hyun;Lee, Bu-Heung;Cho, Jae-Keun;Yoon, Won-Koung;Kim, Won;Kim, Hee-Jung;Kim, Eun-Mi;Kim, Ki-Seuk;Park, Choi-Kyu
    • Korean Journal of Veterinary Service
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    • v.37 no.1
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    • pp.79-84
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    • 2014
  • Two Hahns Macaws (Ara nobilis nobilis), three and nine-month-old, with a three-weeks history of weight loss and anorexia were diagnosed presumptively with proventricular dilatation disease (PDD) by radiographic examination. The birds were treated with antimicrobials, analgesics, and fluid administration. However, these birds died three weeks after the first signs of PDD. At necropsy, the birds had severely dilated proventriculus, severe pectoral muscles atrophy, and blood vessels congestion on cortex of cerebrum. The partial matrix gene of avian bornavirus (ABV) was detected by RT-PCR from tissues of the brain, feather calami, and proventiculus of each PDD-suspected birds. This report describes the first detection of ABV in PDD-suspected Hahns Macaw in Korea.

AN ELECTRON MICROSCOPIC STUDIES OF RADIATION EFFECTS ON THE ENAMEL DEVELOPMENT OF THE RAT MOLAR (방사선조사가 백서구치법랑질발육에 미치는 영향에 관한 전자현미경적 연구)

  • Cho Hyo-Suck;You Dong-Soo
    • Journal of Korean Academy of Oral and Maxillofacial Radiology
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    • v.25 no.2
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    • pp.483-499
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    • 1995
  • Mandibular first molars of the rats were undertaken to observe the radiosensitivity of amelogenesis. Twenty four Sprague-Dawley rats received 396cGy radiation with the MK Cell irradiator using Cs-137, and twenty four rats served as control. They were devided into two groups; Group 1 which received radiation at the 14th day after gestation and group 2 which received radiation at the 19th day after gestation. Light Microscopy and Transmitted Electron Microscopy investigation was undertaken in the group 1 at the 15th, 18th, 20th, 22nd(2 days after birth), and 25th day(5 days after birth) after gestation, and in the group 2 at the 21th(birth day), 22nd(2 days after birth), and 25th(5 days after birth) day after gestation. The following histopathologic findings were obtained. 1. Compared with control group, experimental group showed a delayed formation of enamel and dentin due to slow rate of differentiation of undifferentiated mesenchymal cells. 2. In the experimental groups, the arrangement of the inner enamel epithelium was irregular and there were many vacuoles in the cytoplasm. There were dilated rER and mitochondria, increase of the intercellular space, and loss of the cellular polarity. 3. In the group 1, early enamel without Tomes' process, and early organic matrix was observed at the 25th day after gestation. 4. In the group 2, histopathologic changes were similar to those of the group 1, but the degree of changes was more severe than that of the group 1.

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AN EXPERIMENTAL STUDY ON PULPAL RESPONSE TO THE PHOSPHORIC ESTER CEMENT IN DOGS (인산(燐酸) ESTER계(系) 접착성(接着性) 시멘트의 치수반응(齒髓反應)에 관(關)한 실험적(實驗的) 연구(硏究))

  • Lee, Hye-Young;Choi, Dae-Gyun;Choi, Boo-Byung;Park, Nam-Soo
    • The Journal of Korean Academy of Prosthodontics
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    • v.24 no.1
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    • pp.125-138
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    • 1986
  • It is considered that etching solution or material itself of phosphoric ester cement will induce not a little pulpal irritation, if applied directly onto unsealed dentinal tubules. This study was designed to confirm above consideration by comparing two different conditions between $Ca(OH)_2$-based and non-$Ca(OH)_2$-based group. Posterior teeth of 15 male dogs were selected for this experiment. One experimental group was filled with cement after $Ca(OH)_2$-basing and enamel-etching, the other experimental group after enamel etching without $Ca(OH)_2$-basing. And both of two experimental groups were observed at 2 hours, 15 hours, 3 days, 1 week, 2 weeks, 4 weeks, and 6 weeks after filling. The findings reached to the following conclusions histologically. 1. In both groups, the damaged odontoblasts were atrophied and eventually disappeared. 2. In non-based group at early stage, odontoblasts were severely atrophied and defective areas were appeared between odontoblast cell layers. However, in based group, the odontoblasts were arranged slight irregularly. 3. In non-based group, a small number of undifferentiated cells below the odontoblast cell layers started to appear at 1 week after filling. However, in based group, the undifferentiated cells were appeared at 15 hours after filling. 4. In non-based group, formation of reparative dentin was not begun until late stage of experiment. However, in based group, formation of reparative dentin matrix was begun at 2 weeks after filling and very thickened reparative dentin was formed at 6 weeks after filling. 5. In odontoblast cell layers of both groups, dilated capillaries were observed. 6. Argyrophilic fibers were reticularly condensed in zone of Weil, and they were connected to the pulp tissue and dentin.

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A Case of Neuroblastoma Presenting with Sudden Blindness (실명을 주소로 한 신경아세포종 1예)

  • Ma, In-Youl;Hah, Jeong-Ok;Kim, Chun-Dong;Lee, Tae-Sook
    • Journal of Yeungnam Medical Science
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    • v.2 no.1
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    • pp.259-264
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    • 1985
  • Neuroblastoma is the most common extracranial solid tumor of childhood which presents various clinical symptoms depending on the primary and metastatic sites. However, it has been rarely reported that sudden onset of blindness was the chief complaint of neuroblastoma. A four years old boy was admitted to the Yeungnam University Hospital with the chief complaint of a sudden onset of blindness due to a distant metastasis of abdominal neuroblastoma to the sphenoid sinus. On admission, both side pupils were dilated without light reflex, fundoscopy showed pale optic disk, electroretinogram was subnormal and visual evoked potential showed no response. The liver was palpable in $3{\frac{1}{2}}$ finger breadth from the right costal margin and adult fist sized mass was palpable in the right flank. Skull X-ray showed destructed sphenoid bone and clinoid process and brain CT scan showed tumor mass in the sphenoid sinus and left orbit. Ultrasonogram and CT scan of the abdomen showed large tumor masses around the right kidney and para-aortic and retropancreatic lymph node. IVP showed displaced right calyceal system with preserved contour. Left supraclavicular lymph node which appeared after admission was biopsied and it showed poorly differentiated neuroblasts. He was treated according to the multiagent chemotherapy schedule for stage IV neuroblastoma patient of children's cancer study group. Abdominal tumor masses and sphenoid sinus mass were markedly reduced after 2 courses of the combination chemotherapy of cyclophosphamide, vincristine, DTIC, adriamycin and VM-26. Eventhough the blindness was not improved, the patient has been in good clinical condition.

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Coronary Artery bypass Surgery Using Skeletonization Technique of Left Internal Mammary Aartery (관상동맥 우회술시 근막 및 정맥 등 주위조직이 없는 좌내흉동맥편의 이용)

  • Choe, Jong-Beom;Lee, Sang-Yun
    • Journal of Chest Surgery
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    • v.28 no.7
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    • pp.671-677
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    • 1995
  • Skeletonization of the internal mammary artery [IMA during myocardial revascularization procedures may provide some advantages, compared with the pedicle graft of the artery. In 17 patients undergoing IMA grafting by skeletonization technique, flow through the artery was measured on mean arterial pressure of 50-55 mmHg immediately after cardiopulmonary bypass started [first flow and just before its anastomosis to left anterior descending artery [second flow . In 16 patients except 1 patient whose graft was injured during mobilization, the first flow of IMA graft was 32.3 $\pm$ 7.4 ml/min and the second flow increased to 59.6$\pm$25.9 ml/min without any treatment and the site for anastomosis of the IMA graft was more than 1.0 cm above the bifurcation. On the basis of previous clinical studies, the flow of the skeletonized IMA was greater than that of the pedicle graft [59.6 $\pm$ 25.9 ml/min versus 37.7$\pm$ 14.1 ml/min, p < 0.05 . In comparison between the skeletonized IMA and the IMA graft intraluminally dilated with papaverine solution, there was no significant difference between two flows[59.6 $\pm$25.7 ml/min versus 74.7 $\pm$31.4 ml/min, not significant , but the former showed longer graft and anastomosis of more proximal portion of the graft to left anterior descending artery. In conclusion, the technique of internal mammary artery skeletonization has consistently produced a satifactory conduit for myocardial revascularization procedures. We have adopted IMA skeletonization not only because of the flow, diameter, and vessel length obtained but also because of limited perivascular tissue disruption that occurs during the dissection.

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Congenital Aortic Valvular Insufficiency Caused by Abnormal Valvular Structures in a Labrador Retriever Dog (래브라도 리트리버종 개의 비정상 판막 구조에 의한 선천성 대동맥 판막 부전)

  • Moon, Hyeong-Sun;Lee, Seung-Gon;Lee, Sang-Eun;Hyun, Chang-Baig
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.233-237
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    • 2007
  • A 10-month-old intact male Labrador Retriever dog was referred with the primary complaint of exercise intolerance, especially after vigorous exercise. Physical examination revealed split S1 and grade III/VI diastolic regurgitant murmur at the left apex and base, respectively. ECG finding was normal sinus rhythm at rest, but supraventricular tachycardia with bundle branch blocks after exercise. Thoracic radiography revealed dilated ascending aorta with normal range of cardiac silhouette (VHS 10.2). Echocardiography revealed abnormal valvular structures just above the aortic valvular cusps causing aortic regurgitation with a reduction of left ventricular ejection fraction (LVEF). Based on those findings, the case was diagnosed as congenital aortic regurgitation caused by abnormal valvular structures. The dog was managed with diltiazem and exercise restriction. This is a rare case of aortic deformity in dogs.

Proventricular Dilation Disease Concurrent with Ingluvitis in an Indian Ring-necked Parakeet (Psittacula krameri manillensis) (인디안 링넥 앵무새에서 발생한 소낭염을 동반한 선위확장증)

  • Lee, So-Young;Jung, Dong-In;Kim, Ha-Jung;Kim, Ju-Won;Lim, Chae-Young;Kang, Byeong-Teck;Yoo, Jong-Hyun;Kim, Dae-Young;Park, Hee-Myung
    • Journal of Veterinary Clinics
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    • v.24 no.3
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    • pp.437-440
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    • 2007
  • A six-year-old, intact female Indian ring-necked parakeet (Psittacula krameri manillensis) was presented due to chronic weight loss and anorexia. The bird was tentatively diagnosed as proventricular dilation disease (PDD) by radiographic evidences of dilated crop and proventriculus. The patient was symptomatically treated, however the bird was die two months after the first presentation. At necropsy finding, distinctive signs of PDD were noted, and PDD concurrent with ingluvitis was finally diagnosed according to histopatholgic examination.

Extrahepatic Biliary Obstruction Secondary to Traumatic Diaphragmatic Hernia in Dog (개에서의 횡격막 탈장에 의한 간외담도폐색)

  • Yoon, Young-Min;Jeong, Dong-In;Yeon, Seong-Chan;Lee, Hee-Chun
    • Journal of Veterinary Clinics
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    • v.31 no.6
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    • pp.531-534
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    • 2014
  • A 3-year-old intact mixed hunting dog was referred to Gyeongsang Animal Medical Center with the clinical signs of vomiting, dyspnea, anorexia. The dogs had traumatic history that wounded at abdominal wall during the wild boar hunting 2 week ago. The dogs was diagnosed as extrahepatic biliary tract obstruction (EHBO) secondary to traumatic hernia. Diagnosis was based on radiographic features of a diaphragmatic hernia on survey radiography, dilated bile duct in abdominal ultrasonography and complete recovery due to the return of displaced organs into the peritoneal cavity and repair of the diaphragmatic rent. Diagnostic imaging and surgical intervention may result in a favorable outcome in dog with post hepatic obstruction secondary to diaphragmatic hernia. This case emphasizes the strangulation of bile duct by diaphragmatic rupture could be the cause of EHBO.

Ultrasonographic Findings of Pneumatosis Intestinalis in a Dog (개에서 장 기종의 초음파적 특징 1례)

  • Song, Yu-Mi;Lee, Jeong-Yang;Lee, Jung-Woo;Jeung, Woo-Chang;Lee, Young-Won;Choi, Ho-Jung
    • Journal of Veterinary Clinics
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    • v.30 no.2
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    • pp.138-141
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    • 2013
  • A 4-month-old, intact male mongrel dog was presented with trauma (hit by car), astasia, stupor and abdominal pain. Radiographic findings were peritoneal effusion, intestinal dilation, right iliac fracture, and microcardia. Abdominal ultrasonography revealed severely, dilated small intestine containing anechoic fluid in lumen and intramural gas within the wall. The sonographic finding suggested pnematosis intestinalis (PI), and vascular compromise of bowel loop by trauma was strongly suspected. After the dog was dead, postmortem examination revealed discolored and necrotized small intestine strangulated by sublumbar muscle. The detection of echogenic gas bubbles in the wall of bowel using ultrasound may be helpful in detecting PI.

The Experimental Model on Morphological Changes of the Liver Cell Treated with Ethanol (에타놀 처치 간세포(肝細胞)의 형태학적 변화에 대한 실험모델)

  • Lee, Jae-Hyun
    • Korean Journal of Veterinary Research
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    • v.23 no.1
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    • pp.45-56
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    • 1983
  • In order to prepare an experimental model on the basis of morphological changes of liver, 90 rabbits were allocated into four groups and 5, 15, 30 and 50% of ethanol (5ml/day) were dosed to each group for 5, 10, 20, 30, 60 and 90 days, and observed the livers with light and electron microscope. The results obtained are summarized as follows: 1. Light microscopically, the hydropic changes were found in the liver from 10th day after 5% and 15% ethanol ingestion, and these findings were remarkable as time goes by. Necrosis of hepatocytes, however, was most remarkable on 60th and 90th day after ingestion. The proliferation of interlobular connective tissue was found from 30th day in this group. 2. The hydropic necrosis of liver cells was found from 5th day in 30% and 50% ethanol dosing groups. The proliferation of interlobular connective tissues and infiltration of the lymphocytes were observed from 30th day in 30% ethanol ingestion group, however, it was found from 20th day in 50% ethanol ingestion group. 3. Electron microscopically, the appearance of various lipid droplets and dilated rER, slightly increased sER and glycogen field, dense mitochondria with scanty cristae and hydropic change were observed on 5th day after 5% ethanol ingestion. These findings were more remarkable on 30th and 90th day. Especially, disorganized outer and inner membrane of mitochondria and autophagic vacuoles were appeared on 90th day. The similar findings were also found in 15%, 30% and 50% ethanol ingestion groups, however, the appearance of fibrillar and myelin-like structures were observed from 30th day after 15% ethanol ingestion. The severe hydropic change and increased sER were observed on 90th day after 15% ethanol ingestion. From 5th day after 30% and 50% ethanol ingestion these findings were more remarkable.

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