• 제목/요약/키워드: Digestive activity

검색결과 305건 처리시간 0.025초

무당벌레 소화기관으로부터 산성 단백질 분해효소를 생산하는 Staphylococcus sp. CB2-3의 분리 및 특성 (Isolation and Characterization of Acid Protease Produced by Staphylococcus sp. CB2-3 from Digestive Organ of Harmonia axyridis)

  • 김세종;황경숙
    • 미생물학회지
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    • 제47권3호
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    • pp.255-262
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    • 2011
  • 무당벌레 소화기관으로부터 단백질 분해 우수세균 6균주를 분리하였다. 단백질 분해세균의 16S rRNA 유전자 염기서열을 해석하여 계통학적 특성을 검토한 결과, Staphylococcus sciuri subsp. sciuri (3균주), Bacillus subtilis (1균주), Bacillus thuringiensis (2균주)로 확인되었다. 이들 균주 중 pH 5.0 배지에서 58.5 U/ml의 높은 효소 활성을 나타내는 Staphylococcus sp. CB2-3을 최종 선발하였다. 효소의 특성을 조사한 결과, pH 4.0-6.0에서 높은 활성을 나타내어 산성 단백질 분해효소임이 확인되었다. 효소의 최적 반응 온도는 $40^{\circ}C$ 이었으며, $30-50^{\circ}C$의 범위에서 80% 이상의 효소 활성을 유지하였다. Staphylococcus sp. CB2-3 균주의 생육과 효소 활성을 위한 최적의 배지성분을 조사하였다. 탄소원으로 0.5% 솔비톨을 첨가하였을 때 효소 활성이 2배로 증가되었으며, 질소원으로 0.5% 탈지유를 첨가한 경우 효소 활성이 2.5배 증가되는 것으로 나타났다. 무기염류로는 KCl, $K_2HPO_4$, $FeSO_4$를 첨가하였을 때 효소 활성이 가장 높은 반면에 2가 금속이온인 $Co^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Cu^{2+}$를 첨가하였을 때는 균의 성장과 효소 활성이 심하게 저해되는 것으로 나타났다.

The Effects of Fiber Source on Organ Weight, Digesta pH, Specific Activities of Digestive Enzymes and Bacterial Activity in the Gastrointestinal Tract of Piglets

  • Ma, Yongxi;Li, Defa;Qiao, S.Y.;Huang, C.H.;Han, In K.
    • Asian-Australasian Journal of Animal Sciences
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    • 제15권10호
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    • pp.1482-1488
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    • 2002
  • The aim of this study was to explore the effects of fiber sources on gut development and bacterial activity in the gastrointestinal tract of piglets. Eighteen crossbred (Duroc${\times}$Landrace${\times}$Yorkshire) barrows were fed a basal diet based on corn plus soybean meal or similar diets in which a portion of the corn and soybean was replaced by 5% wheat bran or 5% sugar beet pulp. The results indicate that pigs fed diets containing 5% wheat bran or 5% sugar beet pulp had lower liver weights than control pigs (p<0.01). The relative weight of the pancreas in pigs fed diets containing 5% sugar beet pulp was greater than that of control pigs or pigs fed diets containing 5% wheat bran (p<0.05). The pH of the ileal digesta of pigs fed the diet containing 5% wheat bran was higher than that of control pigs or pigs fed the diet containing 5% sugar beet pulp (p<0.05). The lipase activity in the distal jejunum, proximal, and distal ileum of pigs fed the control diet was higher than that of pigs fed the diets containing 5% wheat bran or 5% sugar beet pulp (p<0.05). The concentration of volatile fatty acids anterior to the caecum was greater for the pigs fed the diet containing 5% sugar beet pulp, while the concentration of volatile fatty acids posterior to the ileum was greater for the pigs fed the diet containing 5% wheat bran. This means that sugar beet pulp increased the bacterial fermentation precaecum, while wheat bran increased the bacterial fermentation post-ileum. The concentration of bacterial nitrogen and bacterial protein/total protein in ileal digesta of pigs fed the control diet was higher (p<0.05) than that of pigs fed the diets contained either fiber source. Bacterial protein/total protein in the feces of pigs fed the diet containing 5% sugar beet pulp was higher than that of pigs fed the control diet. This means that inclusion of 5% wheat bran or sugar beet pulp in diets influenced the development of the digestive tract of piglet. The mechanism by which dietary fiber reduced specific activity of lipase needs further consideration. Dietary fiber influenced the bacterial activity in the digestive tract of piglets, sugar beet pulp increased the fermentation in the upper gastrointestinal tract, and while wheat bran increased the fermentation in the lower gastrointestinal tract.

가잠유충의 소화액 Proteinase 및 중장조직 Sucrase의 활성에 미치는 사료조성(당과 단백질과의 량비) 및 4 면기보호온도의 영향 (Effect of the Dietary Composition (quantitative ratio between carbohydrate and protein) and the Rearing Temperature during the 4th Moulting Period on Proteinase Activity of Digestive Juice and Sucrase Activity of Midgut Tissue in the Silkworm, Bombyx mori L.)

  • 문재유
    • 한국잠사곤충학회지
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    • 제15권1호
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    • pp.9-14
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    • 1973
  • 누에의 소화액 proteinase 및 중장조직 Sucrase의 활성에 미치는 영양 및 환경의 영향을 알기 위하여, 사료조성중에 당의 함량이 많은 A사료(따라서 단백질의 함량은 적음), 당과백질과의 량비가 약 1 : 2로 적당하다고 생각되는 B사료, 단백질의 함량이 많은 C사료(따라서 당의 함량이 적음)의 3종의 준합성사료를 사용하여 유충을 사육하고, 4면기를 16$^{\circ}C$, $25^{\circ}C$, 32$^{\circ}C$로 각각 보호한 5령 5일째 유충의 소화액 proteinase 및 중장조직 Sucrase의 활성을 조사하였던바 다음과 같은 결과를 얻었다. 1. 유충의 혈당량은 4 면기보호온도와 상관하지 않고 A사료> B사료> C사료의 순으로 많었으나 소화액 proteinase 및 중장조직 Sucrase의 활성은 C사료> B사료 >A사료의 순으로 강하였다. 2. 유충의 소화액 proteinase 활성은 A,B,C의 각 사료에 있어서 대개 같은 경향으로, 32$^{\circ}C$ 보다 16$^{\circ}C$에 있어서 강하였으나 혈당량의 변화와는 상반되는 경향이 없다. 3. 유충의 소화액 Sucrase 활성은 A,B,C의 각 사료에 있어서 같은 경향으로, 32$^{\circ}C$$25^{\circ}C$ >16$^{\circ}C$의 순으로 강하였으며 또한 혈당량의 변화와 같은 경향이었다. 4. 유충의 소화액 proteinase 활성은 사료조성과 4 면기보호온도와의 교호작용이 웅에서만 인정되었으나 중장조직 Sucrase 활성은 교호작용이 자웅 다같이 인정되었다.

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Effects of Flower Thinning Formulation on Activities of Digestive Enzymes and Acetylcholine Esterase in Honey bee Apis mellifera

  • Hemayet Jahan, S.M.;Shim, Jae-Kyoung;Son, Tae-Gwon;Jo, Jaedoo;Choi, Cheul;Lee, Kyeong-Yeoll
    • Current Research on Agriculture and Life Sciences
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    • 제32권2호
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    • pp.63-66
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    • 2014
  • The effects of a newly developed flower thinning formulation (FTF) on the vitality of the honey bee Apis mellifera were examined by measuring the activities of various digestive enzymes in adult worker bees. First, direct spraying of the FTF solution did not cause any behavioral changes or lethal effects for the honey bees based on 24 h observation. Second, oral ingestion of a sugar solution containing the FTF did not produce any significant change in the activities of amylase, proteinases, lipase, or acetylcholine esterase (AChE) in the worker bees 6 h or 24 h after treatment. Meanwhile, a commercial formulation containing sulfur compounds showed slightly reduced activities for several digestive enzymes and AChE, although no behavioral disturbance. Thus, the results of the present study suggest that the FTF is not toxic for honey bees, in terms of contact and ingestion. Therefore, this newly developed FTF can be used for flower thinning without any detrimental effects on pollinating insects.

비장도인법(脾腸導引法)을 적용한 복부 팽만감 주소의 기능성소화불량 증례보고 (Spleen Daoyin Method (脾腸導引法) for Functional Dyspepsia Patients: A Case Report)

  • 정해인;하나연;김진성
    • 대한한방내과학회지
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    • 제42권6호
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    • pp.1331-1340
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    • 2021
  • A 42-year-old patient with functional dyspepsia had suffered from early satiation and postprandial fullness for 1 year despite Western medicine treatment. We treated her with the Spleen Daoyin method, along with acupuncture, moxibustion, and herbal medicine and scored her symptoms using a 4-point Functional Dyspepsia-related Quality of Life (4p-FD-QoL) questionnaire, the Gastrointestinal Symptom Rating Scale (GSRS), and a visual analogue scale (VAS). A 7-day Spleen Daoyin treatment decreased her 4p-FD-QoL score from 61 to 51, with a marked decrease in the emotional part (18 to 10). Her GSRS score did not change, but her bloating score decreased from 3 to 1. Her VAS score decreased from 6.6 to 4.3 for upper abdomen discomfort, but lower abdomen discomfort was unchanged. The patient showed high compliance and satisfaction with exercise therapy and reported no adverse events. Spleen Dayoin may be a useful therapy for functional dyspepsia, especially when accompanied by psychological problems.

수종(數種) 한약(漢藥)의 항균작용(抗菌作用)에 대(對)하여 (Antibacterial Activities of Some Herb Drugs)

  • 최영태
    • 생약학회지
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    • 제17권4호
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    • pp.302-307
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    • 1986
  • Sixteen kinds of Chinese herb drugs using in digestive system have screened for antibacterial activity. It was revealed that all of the herb drug extracts showed antibacterial activity in one or more strains of microbials selected in this experiments. It is noteworthy that Aconitum trilobum showed inhibition of Klebsiella pneumoniae.

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Effects of Diet Composition on Digestive Activities of Enzyme in Lensky Sturgeon

  • Nevalyonny A. N.;Zaitsev V. F.;Korostelyov S. G.
    • 한국양식학회지
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    • 제9권2호
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    • pp.167-168
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    • 1996
  • Experiments were conducted to determine the activities of protease and carbohydrase in growing lensky sturgeon fed with three different diets containing various concentrations of protein and carbohydrate. Neutral pretense activity from growing lensky sturgeon the protein diet (predo-minary, almost $100\%$ protein) was lower then those from fish fed the other diets during the experimental period. The results may indicate that the level of pretense activity is inversely related to the level of protein in the diets.

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