• 한국수산과학회지
• /
• 제29권6호
• /
• pp.770-778
• /
• 1996

A rapid and economical method of simultaneous extraction of DNA and RNA from seaweeds has been developed by the use of lithium chloride. Lithium chloride facilitates the softening of cell walls resulting in a decrease in both compressive and tensile modulus of elasticity. The DNA was characterized by high molecular weight larger than 27 kb and a relative lack of carbohydrate and protein contamination. The DNA and RNA extracted by the method from many seaweeds were of sufficient quality to be used as a template for per amplification with a plant intergenic gene primer set, for RAPD analysis with arbitrary primers, and for differential display with arbitrary primers in the morphologically distinct regions of the matured Porphyra thallus. The cDNA polymorphism indicated that the reproductive tissue types (male, female, patch) had a relatively high degree of similarity; the vegetative tissue types (dividing, non-dividing) also showed a similar pattern with respect to each other. Holdfast tissue had very low similarity with the other tissues, but appeared most similar to vegetative non-dividing tissue type.

• KSBB Journal
• /
• 제14권6호
• /
• pp.702-706
• /
• 1999

대표적인 양식 해조류인 방사무늬 김 엽체를 대상으로 하여 산 처리에 의한 유전형질의 표현 변화를 differential display기법으로 비교하여 보았다. 방사무늬 김 엽체를 0.05% HCl을 첨가한 해수(pH 3.0)에서 5분간 처리한 후 각각 10분, 30분, 60분 그리고 4시간동안 멸균 해수에서 정치 배양시키면서, RNA를 추출하여 cDNA합성, PCR 증폭, agarose gel 전기영동 및 DNA 염기배열을 조사하였다. 그 결과 arbitrary primer OPA 1(CAGGCCCTTC)을 사용하여 differential display한 경우, 산 처리 후 30분간 멸균 해수에서 정치 배양한 엽체에서 특이적으로 RNA 합성이 일어나지 않은 유전자를 분리할 수 있었으며, 그 염기서열을 비교한바 이 유전자 fragment(605 bp)는 dethiobiotin synthetase 유전자와 93%의 높은 상동성을 가진 것으로 나타났다.

• 한국정보통신학회:학술대회논문집
• /
• 한국해양정보통신학회 2007년도 추계종합학술대회
• /
• pp.761-764
• /
• 2007

본 논문은 저전압 차동 신호 방식 (Low-Voltage Differential Signaling, LVDS)의 응용을 위한 차동 전송 접속 경로의 분석 및 설계 최적화 방법을 제안한다. 차동 전송 경로 및 저전압 스윙 방법의 발전으로 인해 LVDS 방식은 데이터 통신 분야, 고 해상도 디스플레이 분야, 평판 디스플레이 분야에서 매우 적은 소비전력, 개선된 잡음 특성 및 고속 데이터 전송률을 제공한다. 본 논문은 차동 flexible printed circuit board (FPCB) 전송선에서 선 폭, 선 두께 및 선 간격과 같은 전송선 설계 변수들의 최적화 기법을 이용하여 직렬 접속된 전송선들에서 발생하는 임피던스 부정합과 신호 왜곡을 감소시키기 위해 개선 모델과 새로이 개발된 수식을 제안한다. 이러한 차동 FPCB 전송선의 고주파 특성을 평가하기 위해 주파수 영역에서 full-wave 전자기 시뮬레이션, 시간 영역 시뮬레이션 및 S 파라미터 시뮬레이션을 각각 수행하였다.

• 한국전기전자재료학회논문지
• /
• 제29권2호
• /
• pp.101-105
• /
• 2016

The basic characteristics of glass are highly fragile and brittle consequences the ultimate purpose of glass manufacturing is to make a transparent glass with complex shape. In order to solve this problem, mechanical properties of glass can be increased by crystallization of its amorphous glass. However, glass-ceramics has become opaque through crystallization process due to the distracted interface of glass by precipitated particles. This study has been investigated thermal processing conditions of LAS transparent glass-ceramic by using DTA (differential thermal analysis), in order to control size of precipitated particle and then fabricate transparent glass-ceramic. DTA indicated that crystallization peak area was declined with increased nucleation temperature. Subsequently, we have been established optimum temperature for crystallization depending on the nucleation temperature. The transmission and thermal expansion were measured after crystallization, and the size of precipitated particle was identified in range of 20~100 nm by FE-SEM. In addition, by setting the optimized crystallization condition, with high transmission and low thermal expansion glass was synthesized through this experiment.

• BMB Reports
• /
• 제30권4호
• /
• pp.292-295
• /
• 1997

Exposure of seedling of rice (Oriza sativa cv.Dongin) to cold stress ($6^{circ}C$, 7day) induced differential gene expression. Differentially expressed polyadenylated RNA induced by low temperature were isolated and identified from the leaves of rice (Oriza sativa cv.Dongin) seedling by using the technique, differential display of reverse transcription through polymerase chain reaction (DDRT-PCR). Four bands of cDNAs were differentially displayed on the PAGE gel through DDRT-PCR, and among them three bands were those of overexpressed genes while one band was of an underexpressed gene One of the overexpressed cDNA was characterized. The size of the DDRT-PCR product was found to be about 200 bp. The sequence of the cloned DNA was compared with those of GenBank through a BLAST E-Mail server, and it was found to have no homologies in the nucleotide sequence with that of any known DNA: therefore, it was designated as RC101 The expression of the cold-stress induced-gene, RC101, was sustained with Northern Blot analysis by using the cloned DDRT-PCR product as a probe.

• Asian-Australasian Journal of Animal Sciences
• /
• 제18권6호
• /
• pp.767-771
• /
• 2005

In order to understand the molecular basis of chicken heterosis in reproduction traits, mRNA differential display (DDRT-PCR) methods were used to analyze the differential gene expression of ovary tissue between hybrids and their parental lines in a 4${\times}$4 diallel cross, involving 4 chicken breeds, which were White Plymouth Rock (E), CAU Brown (D), Silkies (C) and White Leghorn (A). Total of 331 differential displayed cDNA bands from 1,161 were displayed in the 4${\times}$4 diallel cross combinations with 30 pairs of primers, which shows the differences of gene expression between hybrids and their parental lines were very obvious in quantity and quality. Seven types of differential expression patterns were found: Co-dominance expressed pattern (T1), under-expression of parental fragments in hybrids (T2), over-expression of parental fragments in hybrids (T3), hybrid-absence expressed pattern (T4), single parentspecific expressed pattern (T5), dominant expression fragments of single parent in hybrids (T6), hybrid-specific expressed pattern (T7). Correlation analysis indicated that there were significant correlations between the pattern of T3 and the heterosis percentage of egg number of 32-week and 42-week old chickens(p<0.01), while there were negative significant correlations between the pattern of T7 and the heterosis percentage of egg number of 32-week and 42 week-old birds (p<0.01).

• 한국정보통신학회논문지
• /
• 제12권5호
• /
• pp.879-886
• /
• 2008

본 논문에서는 저전압 차동 신호(Low-Voltage Differential Signaling, LVDS) 전송방식의 응용을 위한 차동 전송 접속 경로의 분석 및 설계 최적화 방법을 제안한다. 차동 전송 경로 및 저전압 스윙 방법의 발전으로 인해 LVDS 방식은 데이터 통신 분야, 고해상도 디스플레이 분야, 평판 디스플레이 분야에서 매우 적은 소비전력, 개선된 잡음 특성 및 고속 데이터 전송률을 제공한다. 본 논문은 차동 유연성 인쇄회로 보드(flexible printed circuit board, FPCB) 전송선에서 선폭, 선두께 및 선 간격과 같은 전송선 설계 변수들의 최적화 기법을 이용하여 직렬 접속된 전송선에서 발생하는 임피던스 부정합과 신호왜곡을 감소시키기 위해 개선 모델과 새로이 개발된 수식을 제안한다. 이러한 차동 FPCB 전송선의 고주파 특성을 평가하기 위해 주파수 영역에서 전파(full-wave) 전자기 시뮬레이션, 시간영역 시뮬레이션 및 S 파라미터 시뮬레이션을 각각 수행하였다. $17.5{\mu}m$과 $35{\mu}m$의 전송선의 경우, 전극 폭에서의 약 10% 변화가 차동 임피던스에서의 약 6%와 5.6%의 변화를 각각 보였으나, 전송선 간 간격은 차동 및 특성 임피던스에서의 영향을 주지 않음을 확인하였다. 또한 전송선 간격이 증가할수록 상호 인덕턴스 및 커패시턴스가 감소하기 때문에 누화 잡음을 감소시키기 위해 신호 전송선간의 간격을 $180{\mu}m$ 이상 유지 해야함을 확인하였다.

• BMB Reports
• /
• 제39권1호
• /
• pp.26-36
• /
• 2006

Differential Display PCR technique (DD-PCR) was used for the analysis of altered gene expression in hemocytes of Vibrio harveyi-infected Penaeus monodon. Forty-four combinations of arbitrary and oligo(dT) primers were used to screen for differentially expressed genes. A total of 79 differentially expressed bands could be identified from 33 primer combinations. These included 48 bands (61%) whose expression level increased and 31 bands (39%) decreased after V. harveyi challenge. Subsequently, forty-eight differential display fragments were successfully reamplified and cloned. A total of 267 clones were randomly selected and sequenced. The sequence analysis showed that 85 (31%) out of 267 clones were matched with sequences in the GenBank database which represented 24 different genes with known functions. Among the known genes, glucose transporter 1, interferon-related developmental regulator 1, lysozyme, profilin, SERPINB3, were selected for further confirmation of their differentially expression patterns by real-time PCR. The results showed increasing in expression level of the selected genes in shrimp hemocytes after microbial challenge suggesting the involvement of such genes in bacterial response in shrimp. The anti-lipopolysaccharide factor type 3 (ALFPm3) gene, previously reported in P. monodon (Supungul et al., 2002) was found among the up-regulated genes but diversity due to amino acid changes was observed. Increase in ALFPm3 transcripts upon V. harveyi injection is in accordance with that found in the previous study.

• BMB Reports
• /
• 제37권1호
• /
• pp.1-10
• /
• 2004

DNA and RNA quantifications are widely used in biological and biomedical research. In the last ten years, many technologies have been developed to enable automated and high-throughput analyses. In this review, we first give a brief overview of how DNA and RNA quantifications are carried out. Then, five technologies (microarrays, SAGE, differential display, real time PCR and real competitive PCR) are introduced, with an emphasis on how these technologies can be applied and what their limitations are. The technologies are also evaluated in terms of a few key aspects of nucleic acids quantification such as accuracy, sensitivity, specificity, cost and throughput.

• 한국통신학회논문지
• /
• 제25권10B호
• /
• pp.1832-1840
• /
• 2000

The error diffusion algorithm is good for reproducing continuous images to binary images. However the reproduction of edge characteristics is weak in power spectrum an analysis of display error. In this paper an edge enhanced error diffusion method is proposed to improve the edge characteristic enhancement. Spatial gradient information in original image is adapted for edge enhance in threshold modulation of error diffusion. First the horizontal and vertical second order differential values are obtained from the gradient of peripheral pixels(3x3) in original image. second weighting function is composed by function including absolute value and sign of second order differential values. The proposed method presents a good visual results which edge characteristics is enhanced. The performance of the proposed method is compared with that of the conventional edge enhanced error diffusion by measuring the edge correlation and the local average accordance over a range of viewing distances and the RAPSD of display error.