• 대한미생물학회지
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• 제35권2호
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• pp.149-157
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• 2000

Mycobacterium tuberculosis is capable of growing and survival within macrophage. The purpose of this study was to identify the genes regulated by infection of mycobacteria in human monocytic THP-1 cells. We used the differential display reverse transcriptase polymerase chain reaction (DD RT-PCR) and nothern blot analysis to confirm the differentially expressed genes from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv, heat-killed Mycobacterium tuberculosis H37Rv and live Mycobacterium bovis BCG. Among many up or down-regulated clones, 27 clones were sequenced and compared with known genes on GenBank. Thirteen of over-expressed clones from THP-1 cells infected with live Mycobacterium tuberculosis H37Rv were identical to human prothymosin alpha, eight were novel clones and six clones showed homology with Human ferritin H chain, Esherichia coli bgl, Mouse RNA-dependent EIF-2 alpha kinase, E. coli htrL, Hyaluronan receptor and T cell receptor. Our result suggests that Mycobacterium tuberculosis might regulate prothymosin alpha gene transcription in monocytic THP-1 cell.

• 한국수정란이식학회지
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• 제24권2호
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• pp.77-87
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• 2009

To understand molecular and cellular mechanisms of many gene products in the female reproductive organs including the ovary and uterine endometrium as well as during embryo development, researchers have developed and utilized many effective methodologies to analyze gene expression in cells, tissues and animals over the last several decades. For example, blotting techniques have helped to understand molecular functions at DNA, RNA and protein levels, and the reverse transcription-polymerase chain reaction (RT-PCR) method has been widely used in gene expression analysis. However, some conventional methods are not sufficient to understand regulation and function of genes expressed in very complex patterns in many organs. Thus, it is required to adopt more high-throughput and reliable techniques. Here, we describe several techniques used widely recently to analyze gene expression, including annealing control based-PCR, differential display-PCR, expressed sequence tag, suppression subtractive hybridization and microarray techniques. Use of these techniques will help to analyze expression pattern of many genes from small scale to large scale and to compare expression patterns of genes in one sample to another. In this review, we described principles of these methodologies and summarized examples of comparative analysis of gene expression in female reproductive organs with help of those methodologies.

• 폴리머
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• 제32권6호
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• pp.587-592
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• 2008

고분자량 폴리 (비닐 알코올) (poly(vinyl alcohol), PVA)에 전지빔 (electron beam, EB)을 조사하여 상처치료용 드레싱으로 응용이 기대되는 수화젤을 성공적으로 제조하였다. 다양한 겔화율, 물에서의 팽윤도, 겔강도, 및 신도를 가지는 수화젤을 제조하기 위해 수평균 중합도($P_n$)가 1700과 4000인 두 종류의 PVA를 이용하였으며, PVA 수용액 농도와 EB 조사선량은 5$\sim$20%와 30$\sim$100 kGy의 범위로 각각 조절되었다. 제조된 PVA 수화젤의 겔화율과 겔강도는 PVA의 분자량과 수용액의 농도 및 EB 조사선량이 증가함에 따라 증가하였으나, 반면에, PVA 수화젤의 팽윤도와 신도는 감소하였다. PVA 수화젤의 가교밀도에 따른 열적특성과 결정성의 변화는 differential scanning calorimetry와 X-ray diffraction을 이용하여 분석되었다.

• 한국균학회지
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• 제26권3호통권86호
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• pp.314-320
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• 1998

mRNA의 differential display방법에 의해 여름느타리 자실체의 상처 또는 자외선 처리시 발현되는 약 0.4kb의 cDNA 단편을 분리하였다. 이 cDNA 단편의 염기서열 분석결과 세포분열 촉진에 관여하는 cdc2-related protein kinase gene과 상당부분 유사성을 보였으며 RT-PCR 방법을 이용한 분리 유전자의 발현 실험을 통해 이 유전자가 정상 생장 환경에서는 갓, 대, 균사 등 모든 부위에서 기본적인 발현상태를 유지하고 있으며 기계적 상처 또는 자외선 처리에 의해 그 발현이 증폭됨을 확인하였다. 이러한 결과를 통해 향후 분리된 유전자의 연구를 통한 버섯 병 방어 관련 신호 전달 체계 분석에 대한 가능성을 검토해 보았다.

• 반도체디스플레이기술학회지
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• 제22권4호
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• pp.142-147
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• 2023

This paper presents a novel design of a differential patch antenna for 60-GHz millimeter-wave applications. The design process of the back-to-back (BTB) patch antenna is based on the conventional single-patch antenna. The initial design of the BTB patch antenna (Type-I) has a patch size of 0.66 × 0.98 mm² and a substrate size of 0.99 × 1.48 mm². It has a gain of 1.83 dBi and an efficiency of 94.4% with an omni-directional radiation pattern. A 0.4 mm-thick high-resistivity silicon (HRS) is employed for the substrate of the BTB patch antenna. The proposed antenna is further analyzed to investigate the effect of substrate size and resistivity. As the substrate resistivity decreases, the gain and efficiency degrade due to the substrate loss. As the substrate (HRS) size decreases approaching the patch size, the resonant frequency increases with a higher gain and efficiency. The BTB patch antenna has optimal performances when the substrate size matches the patch size on the HRS substrate (Type-II). The antenna is redesigned to have a patch size of 0.81 × 1.18 mm² on the HRS substrate in the same size. It has an efficiency of 94.9% and a gain of 1.97 dBi at the resonant frequency of 60 GHz with an omni-directional radiation pattern. Compared to the initial design of the BTB patch antenna (Type-I), the optimal BTB patch antenna (Type-II) has a slightly higher efficiency and gain with a considerable reduction in antenna area by 34.8%.

• 한국재료학회지
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• 제29권11호
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• pp.727-733
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• 2019

In this study, the glass melting properties are evaluated to examine the possibility of using refused coal ore as replacement for ceramic materials. To fabricate the glass, refused coal ore with calcium carbonate and sodium carbonate in it (which are added as supplementary materials) is put into an alumina crucible, melted at $1,200{\sim}1,500^{\circ}C$ for 1 hr, and then annealed at $600^{\circ}C$ for 2 hrs. We fabricate a black colored glass. The properties of the glass are measured by XRD (X-ray diffractometry) and TG-DTA (thermogravimetry-differential thermal analysis). Glass samples manufactured at more than $1,300^{\circ}C$ with more than 60 % of refused coal ore are found by XRD to be non-crystalline in nature. In the case of the glass sample with 40 % of refused coal ore, from the sample melted at $1,200^{\circ}C$, a sodium aluminum phosphate peak, a disodium calcium silicate peak, and an unknown peak are observed. On the other hand, in the sample melted at $1,300^{\circ}C$, only the sodium aluminum phosphate peak and unknown peak are observed. And, peak changes that affect crystallization of the glass according to melting temperature are found. Therefore, it is concluded that glass with refused coal ore has good melting conditions at more than $1,200^{\circ}C$ and so can be applied to the construction field for materials such as glass tile, foamed glass panels, etc.

• 한국정보통신학회논문지
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• 제16권12호
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• pp.2747-2754
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• 2012

본 논문에서 PC 컨텐츠의 활용을 극대화시키는 부가기능을 갖는 다중 LCD(Liquid Crystal Display) 시스템을 개발하였다. 이는 host LCD와 slave LCD로 구성되어 있다. Host LCD는 NTSC(National Television System Committee), PAL(Phase Alternation Line), SECAM(S$\acute{e}$quentiel couleur avec m$\acute{e}$moire) 신호를 받아 영상 및 음성을 데코딩하여 출력한다. 이 데코딩된 신호들을 LVDS(Low Voltage Differential Signaling) 신호로 변환하여 slave LCD단으로 전송을 하는 기능을 갖는다. 그리고 CF 메모리, USB 메모리등을 장착하여 멀티미디어 데이터를 출력하도록 한다. Slave LCD는 host LCD와 달리 튜너부분이 없고 메모리 장착이 되지 않아 자체 TV 신호 수신 및 영상 신호 재생을 하지 못한다. 다만, LVDS 영상 신호를 받아 LCD 팬널에 출력하는 기능만 갖도록 한다. 본 논문에서 개발한 다중 LCD 시스템은 제품이 단순하여 상대적으로 고장률이 낮고, 가격이 저렴하고 제어부분의 간소화로 디스플레이의 전력이 낮으며, host LCD의 채널, 볼륨 및 영상 출력에 대하여 전체 slave LCD를 제어할 수 있는 제품으로서의 가격 및 기능 경쟁력을 갖추고 있다.

• 폴리머
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• 제33권2호
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• pp.164-168
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• 2009

본 연구는 화학적으로 개질된 열가소성 전분(chemically modified thermoplastic starch(CMPS))과 poly(lactic acid)(PLA)와 poly(butylene adipate-co-terephthalate)(PBAT) 블렌드의 형태학, 열적 및 기계적 특성에 미치는 영향에 대해서 연구하였다. PLA/PBAT 블렌드에 CMPS를 이 블렌드의 중량기준으로 10, 20, 30 wt%를 첨가하여 이축압출기로 가공하였다. PLA/PBAT/CMPS 블렌드에서 PLA의 유리전이온도($T_g$)는 CMPS 함량이 증가하여도 큰 변화를 나타내지 않았지만, CMPS의 첨가에 의해 PLA상과 PBAT상 사이의 계면상태가 좋아지는 상용성 있는 형태학을 보여주었다.

• Biomolecules & Therapeutics
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• 제21권2호
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• pp.126-131
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• 2013

Neuropathic pain is a chronic pain disorder caused by nervous system lesions as a direct consequence of a lesion or by disease of the portions of the nervous system that normally signal pain. The spinal nerve ligation (SNL) model in rats that reflect some components of clinical pain have played a crucial role in the understanding of neuropathic pain. To investigate the direct effects of gabapentin on differential gene expression in cultured dorsal root ganglion (DRG) cells of SNL model rats, we performed a differential display reverse transcription-polymerase chain reaction analysis with random priming approach using annealing control primer. Genes encoding metallothionein 1a, transforming growth factor-${\beta}1$ and palmitoyl-protein thioesterase-2 were up-regulated in gabapentin-treated DRG cells of SNL model rats. The functional roles of these differentially expressed genes were previously suggested as neuroprotective genes. Further study of these genes is expected to reveal potential targets of gabapentin.

• 한국환경성돌연변이발암원학회지
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• 제24권2호
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• pp.85-90
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• 2004

Nickel(II) compounds are carcinogenic metals which induce genotoxicity and oxidative stress through the generation of reactive oxygen species. In search of new molecular pathways toward understanding the molecular mechanism of nickel(II)-induced carcinogensis, we performed mRNA differential display analysis using total RNA extracted from nickel(II) acetate-treated normal rat kidney cells (NRK-52E). Cells were exposed for 3 days to 160 and 240 uM nickel(II) concentrations. cDNAs corresponding to mRNAs for which expression levels were altered by nickel(II) were isolated, sequenced, and followed by a GenBank Blast homology search. Specificity of differential expression of cDNAs was determined by RT-PCR and Western blot analysis. Two of them (SH3BGRL3 and FHIT) were down-regulated and one (metallothionein) was up-regulated by nickel(II) treatment. The expression of these mRNAs were nickel(II) concentration-dependent. The levels of FHIT and metallothionein proteins were also consistent with the results for mRNAs. Overall, although the fundamental questions related to function of these genes in nickel(II)-mediated carcinogenicity are not answered, our study suggests that they can be interesting candidates for studies of molecular mechanisms of nickel(II) carcinogenesis.