• Asian-Australasian Journal of Animal Sciences
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• v.14 no.6
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• pp.771-776
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• 2001

A pregnant-induced clone was identified by differential screening from a cDNA library of bovine mammary gland. The clone was identified as a cDNA encoding a polyadenylate binding protein 1 (PABP). The cDNA clone had a total length of 1,911 nucleotides coding for 636 amino acids. The nucleotide sequence of the bovine PABP was 95% and 94% identical to those of human and mouse species, respectively. Comparison of the deduced amino acid sequences of bovine PABP with those of human species showed 100% identity. Induction of the PABP mRNA was observed in bovine mammary tissues at pregnant 7 and 8 months compared to virgin, lactating and involuted states. Expression of the PABP gene was examined in mammary epithelial HC11 cells at proliferating, differentiated and apoptotic conditions. The mRNA levels of PABP gene were similar between proliferating and differentiated cells, but expression levels were very low in apoptotic cells compared to other conditions. Results demonstrate that the PABP gene is induced during pregnancy at which stage mammary epithelial cells are actively proliferating.

• Journal of Microbiology and Biotechnology
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• v.23 no.8
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• pp.1055-1059
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• 2013

The ectomycorrhizal fungus Tricholoma matsutake grows symbiotically with Pinus densiflora. Phenylalanine ammonia-lyase (E.C. 4.3.1.24) catalyzes the conversion of L-phenylalanine to trans-cinnamic acid. The role of fungal phenylalanine ammonia-lyase, however, has not been clear until now. In this study, the gene encoding phenylalanine ammonia-lyase (PAL), which was isolated from T. matsutake, was cloned and characterized. The PAL gene (tmpal) consists of 2,160 nucleotides, coding for a polypeptide containing 719 amino acid residues. The deduced amino acid sequence of tmpal from T. matsutake shows high identity (70%) with that from Laccaria bicolor. Comparative analysis of the PAL genes among T. matsutake and other species of the class Agaricomycetes showed that both active sites and binding sites were significantly conserved among these genes. The transcriptional analysis of the PAL gene revealed a differential gene expression pattern depending on the developmental stages (mycelium, primordium, stipe, pileus, and gills) of T. matsutake. These results suggest that the PAL gene in T. matsutake plays an important role in multiple physiological functions.

• 전자공학회논문지 IE
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• v.49 no.2
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• pp.46-51
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• 2012

Transmit signal of Enhancement Layer in AT-DMB system is received by Coherent Detection, but in Base Layer of AT-DMB, a differential modulation and demodulation is adopted, same as the T-DMB. Especially for the coherent dectection of enhancement layer in AT-DMB system, a channel estimation must be employed. In this paper, I will show that the BER performance of Base-Layer in AT-DMB system will be improved by using the channel estimation information. The suggested method is focusing the constallations after Equalizaing to the nearlest ${\pi}$/4-shift DQPSK constallation points. Simulation results show that for the non-coding environment, the BER performance of AWGN channel, about 2-dB gain can be achieved at $10^{-4}BER$.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.2
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• pp.158-165
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• 2015

Marbling is an important trait regarding the quality of beef. Analysis of beef cattle transcriptome and its expression profile data are essential to extend the genetic information resources and would support further studies on beef cattle. RNA sequencing was performed in beef cattle using the Illumina High-Seq2000 platform. Approximately 251.58 million clean reads were generated from a high marbling (H) group and low marbling (L) group. Approximately 80.12% of the 19,994 bovine genes (protein coding) were detected in all samples, and 749 genes exhibited differential expression between the H and L groups based on fold change (>1.5-fold, p<0.05). Multiple gene ontology terms and biological pathways were found significantly enriched among the differentially expressed genes. The transcriptome data will facilitate future functional studies on marbling formation in beef cattle and may be applied to improve breeding programs for cattle and closely related mammals.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.17 no.2
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• pp.121-131
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• 1992

In this paper, three methods are studied to enhance the performance of SBC ( Sub-Band Coding )schemes for voice signal at the medium bit rate between 12 kbps and If; kbps, and adaptive postfilteritng using human auditory characteristics Is (Bone at the decoder out put. First, GQMF(Generalized Quadrature Mirror Filter ) Is used instead of QME'((Quadrature MirrorFiltcr ) to have better performance. Second, by adaptive bit allocation to each sub-band, speech quality is enhanced and valuable rate ceding If possible. Third, corriparlson study oS thr: coder performance using APCM(Adaptive Pulse Code ModulatioTi) and ADPCM( Adaptive Differentiai Pulse Code Modulatiori) , Indicates that SB AfCM performance better than the other. Adaptive postfiltering at the decoder output enhances the quality of the coded speech. The two proposed postfiltering methods decrease the noise sufficiently at the expense of the low computational load.

• Structural Engineering and Mechanics
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• v.85 no.2
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• pp.147-161
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• 2023

Based on the ideas of variational differential quadrature (VDQ) and finite element method (FEM), a numerical approach named as VDQFEM is applied herein to study the large deformations of plate-type structures under static loading with arbitrary shape hole made of functionally graded graphene platelet-reinforced composite (FG-GPLRC) in the context of higher-order shear deformation theory (HSDT). The material properties of composite are approximated based upon the modified Halpin-Tsai model and rule of mixture. Furthermore, various FG distribution patterns are considered along the thickness direction of plate for GPLs. Using novel vector/matrix relations, the governing equations are derived through a variational approach. The matricized formulation can be efficiently employed in the coding process of numerical methods. In VDQFEM, the space domain of structure is first transformed into a number of finite elements. Then, the VDQ discretization technique is implemented within each element. As the last step, the assemblage procedure is performed to derive the set of governing equations which is solved via the pseudo arc-length continuation algorithm. Also, since HSDT is used herein, the mixed formulation approach is proposed to accommodate the continuity of first-order derivatives on the common boundaries of elements. Rectangular and circular plates under various boundary conditions with circular/rectangular/elliptical cutout are selected to generate the numerical results. In the numerical examples, the effects of geometrical properties and reinforcement with GPL on the nonlinear maximum deflection-transverse load amplitude curve are studied.

• Molecules and Cells
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• v.46 no.1
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• pp.57-64
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• 2023

In eukaryotic cells, a key RNA processing step to generate mature mRNA is the coupled reaction for cleavage and polyadenylation (CPA) at the 3' end of individual transcripts. Many transcripts are alternatively polyadenylated (APA) to produce mRNAs with different 3' ends that may either alter protein coding sequence (CDS-APA) or create different lengths of 3'UTR (tandem-APA). As the CPA reaction is intimately associated with transcriptional termination, it has been widely assumed that APA is regulated cotranscriptionally. Isoforms terminated at different regions may have distinct RNA stability under different conditions, thus altering the ratio of APA isoforms. Such differential impacts on different isoforms have been considered as post-transcriptional APA, but strictly speaking, this can only be considered "apparent" APA, as the choice is not made during the CPA reaction. Interestingly, a recent study reveals sequential APA as a new mechanism for post-transcriptional APA. This minireview will focus on this new mechanism to provide insights into various documented regulatory paradigms.

• Korean Journal of Breeding Science
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• v.42 no.5
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• pp.565-573
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• 2010

Thinning of apple fruitlets is one of the most laborious and important works for the improvement of fruit quality and for the promotion of sufficient flower bud formation to prevent alternate bearing in commercial cultivars. Lateral fruits of self-thinning apple cultivars fall naturally within 30 days after full bloom and only central fruit remains to mature. Differences of gene expression between central fruit and lateral fruit were investigated by differential display (DD) PCR. Partial cDNAs of 30 clones from the central fruit and 24 clones from the lateral fruit were selected for nucleotide sequence determination and homology searches. The levels of transcripts coding for proteins involved in pathogenesis related proteins, senescence, temperature stress, protein degradation, fruit browning, sorbitol metabolism were significantly higher in pedicels of lateral fruit than in pedicels of central fruit. On the other hand, the up-regulation of proteins involved in anthocyanin and flavanol biosynthesis and ethylene synthesis were observed in pedicels of central fruit. In Real time PCR analysis, cytochrome P450 gene was confirmed as showing a higher expression level in lateral fruit than in central fruit. The results of this study indicate that differentially expressed genes are related to self-thinning characteristics in apple tree.

• The Journal of the Korea Contents Association
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• v.16 no.8
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• pp.257-267
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• 2016

The research subject is 8 mothers who put their physical/mentally impaired children in a group home, as the research method, this study conducted an in-depth interview survey. The participants of this study collected data from the in-depth interview on 8 mothers who put their disabled children to the group home. In the result of open coding, total 34 concepts, 28 subcategories and 13 categories were derived. The core category in the selective coding was 'establishing restorative relationship through entering a group home after encountering the limit of nurture.' Practical Strategies include the following; first, it's necessary to provide psychology counselling consequent on a mother's nurturing phase, and this study proposes a program for a father having a child with disability; in addition, there is the necessity of having to arrange the differentiated facility for physically/mentally impaired people, which meets the needs of the relevant people. At a level of policy, this study suggested the necessity of having to take into account the minimization of poverty problem facing a family having a disable child through the caring card, necessity of the use of good-natured card, and medical-social-welfare-based intervention, expansion of facility-touring class installation, and use of adult guardianship system, and differential payment of disabled child nurturing allowance consequent on income quantile, etc.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39A no.12
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• pp.746-755
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• 2014

Compressive sensing (CS) has drawn much interest as a new sampling technique that enables signals to be sampled at a much lower than the Nyquist rate. By noting that the block-based compressive sensing can still keep spatial correlation in measurement domain, in this paper, we propose a novel encoding technique for measurement data obtained in the block-based CS of natural image. We apply discrete wavelet transform (DWT) to decorrelate CS measurements and then assign a proper quantization scheme to those DWT coefficients. Thus, redundancy of CS measurements and bitrate of system are reduced remarkably. Experimental results show improvements in rate-distortion performance by the proposed method against two existing methods of scalar quantization (SQ) and differential pulse-code modulation (DPCM). In the best case, the proposed method gains up to 4 dB, 0.9 dB, and 2.5 dB compared with the Block-based CS-Smoothed Projected Landweber plus SQ, Block-based CS-Smoothed Projected Landweber plus DPCM, and Multihypothesis Block-based CS-Smoothed Projected Landweber plus DPCM, respectively.