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Genotyping of avian pathogenic Escherichia coli by DNA fragment analysis for the differences in simple sequence repeats

  • Han, Mi Na;Byeon, Hyeon Seop;Han, Seong Tae;Jang, Rae Hoon;Kim, Chang Seop;Choi, Seok Hwa
    • Korean Journal of Veterinary Service
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    • v.41 no.4
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    • pp.257-262
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    • 2018
  • Avian pathogenic E. coli (APEC) causes severe economic losses in the poultry farms, due to systemic infections leading to lethal colisepticemia. It causes a variety of diseases from air sac infection to systemic spread leading to septicemia. Secondary infection contains opportunistic infections due to immunosuppression disease. Collibacillosis causes the great problems in the poultry industry in Korea. Thus, it is necessary to identify and classify the characteristics of E. coli isolate of chicken origin to confirm the diversity of symptoms and whether they are transmitted among the farms. Fragment analysis is identify the difference in the number of Variable-Number Tandem-Repeats (VNTRs) for genotyping. VNTRs have repeating structure (Microsatellite, Short tandem repeats; STR, Simple sequence repeats; SSR) in the chromosome. This region can be used as a genetic marker because of its high mutation rate. And various lengths of the amplified DNA fragment cause the difference in the number of repetition of the DNA specific site. The number of repetition sequences indicates the separated size of fragments, so the each fragments can be distinguished by specific samples. The results of the sample show that there is no difference in six microsatellite loci (yjiD, aidB, molR_1, ftsZ, b1668, yibA). There are differences among the farms in relation of the number of repetitions of other six microsatellite loci (ycgW, yaiN, yiaB, mhpR, b0829, caiF). Four (ycgW, yiaB, b0829, caiF) of these six microsatellite loci show statistically significant differences (P<0.05). It means that the analysis using four microsatellite loci including ycgW, yiaB, b0829, and caiF can confirm among the farms. Five E. coli samples in one farm have same SSR repetition at all markers. But, there are significant differences from other farms at Four (ycgW, yiaB, b0829, caiF) microsatellite loci. These results emphasize again that the four microsatellite loci makes a difference in the amplified DNA fragments, enabling it to be used for E. coli genotyping.

Chracteristics of Primary Health Practice and Diagnosis-Cluster Pattern in Health Insurance (의원의 특성에 따른 상병진단군의 분포에 대한 연구)

  • Yoon, Jong-Ryool;Moon, Ok-Ryun;Huh, Jung;Kim, Chang-Yup
    • Health Policy and Management
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    • v.3 no.2
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    • pp.100-129
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    • 1993
  • This study is designed to find out some intra-clinic factors affecting the content of practice provided by primary care physicians in Korea, and proposed factors in this study are characteristcs of each private clinc --- physician-related variables(age, sex, specialty), bfed-related variables for inpatient care, laboratory-related variables for precise diagnosis. We have tried to estimate the difference of disease entities cared by each primary care physician according to above factors by analyzin gdisease data claimed during one month(April, 1992) to National Federation of Medical Insurance. The diagnosis codes by ICD-9 in the research disease data were reclassified to 'diagnosis clusters' by virtue of clinical similarities for effective analyses. We have converted frequent-tsing ICD-9 codes to 86 diagnosis clusters, which incorporated 97.4 percents of all ambulatory visits to private clinics. This result means proposed diagnosis-cluster method is effective tool for analysis of the content of ambulatory medical care carried out by primary care physicians. Comparisons and analyses of multiple diagnosis-clusters made on the basis of presented factors were done and the results were as follows; - Major factors affecting the difference between diagnosis-cluster pattern by each variables were phyusician's age, sex, specialty and bed counts of each private clinic for inpatient care and the size of laboratories of each clinic. - Middle aged(30th to 40th) group physicians are providing more comprehensive care than 20th or above 50th aged groups. Male physicians are more adequate for comprehensive care than female physicians, because woman-doctors are providing narrow-spectrum care. The content of practice of obstetricians and gynecologists shows much difference from primary medical practice, and they cannot be included in primary care physician, this study suggested. Pediatricians are also providing short-spectum acre, and nearly all visits to pediatricians were incorporated only 2-3 diagnosis-clusters. General surgeons' practices are very similar to general practioners' or family physicians' practices, the means they are providing primary care rather than special surgical care. And small number of beds(under 5 beds) and only basic(2-3 sorts of)diagnostic apparatuses are sufficient for primary physicians' clinic to carry out primary care. In conclusion, to reinforce primary care department in Korea, there must be support with health policy to expand office-based primary care practice-- with small number of beds for inpatient care and only basic laboratories-- provided by general practitioner of family physician.

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Study on Aged Men's Utilization Status and Satisfaction of Removable Dentures (일부 노인들의 가철성 틀니 사용 실태 및 만족도 조사)

  • Park, Jong-Hee;Park, Ji-Won
    • Journal of Technologic Dentistry
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    • v.34 no.3
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    • pp.291-302
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    • 2012
  • Purpose: It is to survey old men's utilization status of dental clinic and temporary-binding dentures and then to provide basic data to make a program for manufacturing old men's dentures and their teeth health education. Methods: Random selection was made on old men living in Daejeon and Chungnam, and a survey was conducted from April 15 to May 20, 2011. 500 questionnaires were distributed and 420 questionnaires were collected. Using spss 18.0 program, final analysis was made on 363 questionnaires except for 57 questionnaires which had missing value or showed some doubts about the unreliability. Results: 1. With respect to number of natural teeth as per the age, the upper/lower jaws had meaningful differences (P<.001), which means that the older the person is, the smaller number of natural teeth is. In the meantime, there was no meaningful difference according to sex. 2. With respect to kind of prosthetics installed in a mouth as per the age, there was meaningful difference in the upper jaws (P<.05) but there was no meaningful difference in the lower jaws. It was found out that the older the persons are, the more they use removable dentures while the younger the persons are, the more they use fixed partial dentures. 3. With respect to mouth health condition recognized by oneself, it showed bad (44.4%) and good (10.3%),which means recognition as bad is greater, and the age when prosthetics were first used is dispersed variously from below 40 to over 70. 4. Dental care institutions which they mainly used are hospital and clinics (91.5%), and 66.4% of them visited dental clinics lately. Preferential treatment if they go to dentists is as follows in the order of prosthetics (33.9%), gum treatment (24.2%) and pain treatment (14.9%). 5. 68.4% of them revisited dentists after installation of dentures and the reason for revisit is as follows in the order of poorly fitted dentures (35.1%), painful gum 25.9%, regular check-up 25.4% and difficult chewing 9.6%. Conclusion: As our country enters into an aging society, mouth health problem of old men has come to a serious issue, and therefore, a survey was conducted on some old men with respect to their utilization status of dental clinic and removable dentures in order to provide basic data for improvement of quality of their life, maintenance and enhancement of their mouth health. The study result shows that the older they are, the higher the loss rate of teeth as well as needs for prosthetics are, and thus it is thought the study will help to develop mouth health education program which may improve quality of old men's life by recovery of mouth functions declined due to loss of teeth and by regular check-up, education for mouth health control and following-up control of dentures.

Inference of the Probability Distribution of Phase Difference and the Path Duration of Ground Motion from Markov Envelope (Markov Envelope를 이용한 지진동의 위상차 확률분포와 전파지연시간의 추정)

  • Choi, Hang;Yoon, Byung-Ick
    • Journal of the Earthquake Engineering Society of Korea
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    • v.26 no.5
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    • pp.191-202
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    • 2022
  • Markov envelope as a theoretical solution of the parabolic wave equation with Markov approximation for the von Kármán type random medium is studied and approximated with the convolution of two probability density functions (pdf) of normal and gamma distributions considering the previous studies on the applications of Radiative Transfer Theory (RTT) and the analysis results of earthquake records. Through the approximation with gamma pdf, the constant shape parameter of 2 was determined regardless of the source distance ro. This finding means that the scattering process has the property of an inhomogeneous single-scattering Poisson process, unlike the previous studies, which resulted in a homogeneous multiple-scattering Poisson process. Approximated Markov envelope can be treated as the normalized mean square (MS) envelope for ground acceleration because of the flat source Fourier spectrum. Based on such characteristics, the path duration is estimated from the approximated MS envelope and compared to the empirical formula derived by Boore and Thompson. The results clearly show that the path duration increases proportionately to ro1/2-ro2, and the peak value of the RMS envelope is attenuated by exp (-0.0033ro), excluding the geometrical attenuation. The attenuation slope for ro≤100 km is quite similar to that of effective attenuation for shallow crustal earthquakes, and it may be difficult to distinguish the contribution of intrinsic attenuation from effective attenuation. Slowly varying dispersive delay, also called the medium effect, represented by regular pdf, governs the path duration for the source distance shorter than 100 km. Moreover, the diffraction term, also called the distance effect because of scattering, fully controls the path duration beyond the source distance of 300 km and has a steep gradient compared to the medium effect. Source distance 100-300 km is a transition range of the path duration governing effect from random medium to distance. This means that the scattering may not be the prime cause of peak attenuation and envelope broadening for the source distance of less than 200 km. Furthermore, it is also shown that normal distribution is appropriate for the probability distribution of phase difference, as asserted in the previous studies.

Comparative Study on the Growth Condition of Landscape Woody Plants according to the Ground Structure - Focusing on Manseok Beach Town Complex 2, Incheon - (지반구조에 따른 수목 생육상태 비교 연구 - 인천광역시 만석비치타운 단지를 대상으로 -)

  • Cho, Sung-Ho;Han, Bong-Ho;Park, Seok-Cheol
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.25 no.3
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    • pp.63-82
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    • 2022
  • The purpose of this study is to compare growth condition of landscape woody plants growing on the different ground structures in apartment complex. I chose Manseok Beach Town Complex 2, in Manseok-dong, Seo-gu, Incheon which has both natural and artificial ground as a subject site. Analysis of three phases of soil showed that artificial ground had average liquid phase of 30.89%, artificial ground mounding 33.88% and natural ground 24.40%. It means that artificial ground has higher water content than natural ground despite having same earthiness. It is believed that artificial ground is not as well drained as natural ground even though it is connected to the natural ground and has a deep soil depth because of mounding. Comparative study between woody plants on natural ground and those on artificial ground demonstrated that trees on natural ground grew 40.4% compared to those on artificial ground(0.875mm more) in terms of diameter growth. Average diameter growth of trees on natural ground was 3.040mm against 2.165mm for those on artificial ground. All 19 tree species which were measured for root diameter growth showed similar or higher growth on natural ground than on artificial ground. When it comes to growth of height, arborvitae showed highest growth on natural ground, followed by Thuja occidentalis, Pinus strobus, Magnolia denudata, Diospyros kaki and Aesculus turbinata. I measured branch growth and rate of leaf adherence of Pinus strobus. Average annual rate of branch growth of woody plants on natural ground was twice as high as those on artificial ground. I could conclude that ground structure influences branch growth of Pinus strobus. Statistics analysis of tree damage demonstrated significant result, meaning that there is a difference in the average damage rate depending on structure of ground. In order to validate growth difference by planting ground, I conducted T-Test of growth of diameter, root diameter, branch and height on woody plants growing on natural and artificial ground. As a result, it is believed that there is a difference in the growth of trees depending on the ground structure. Putting all these results together demonstrates that woody plants on natural ground generally grow better than those on artificial ground, which means ground structure does have an influence on the environment of growth of trees.

Observation on the shape of the neck -by principal component analysis of the mesurements- (피복 구성을 위한 경부 형태의 관찰)

  • 이연순
    • Journal of the Ergonomics Society of Korea
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    • v.10 no.2
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    • pp.31-42
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    • 1991
  • To understand the shape of the neck in a view of garment planning, principal component analysis has been appliedto the measurement of the neck. The neck surface development and the cross sections of the neck have been observed. The materials consist of the body mearsurements, the neck surface developments and the cross sec- tions of the necks of a total of 108 korean woman students. The difference between the right side and the left side of the neck has not been reconginiged. But the differenece among the height of the front neck point, that of the side neck point and that of the back neck point has been recognized. 2. The initial 41 items have been found having variety and duplication. So two criteria have been made to solve those problems and the selection of 34 items have been made by each criterion. 3. 43 and 34 items have been compared by means of accumulative ratios of contribution and of clearness within the meaning of principal component. As a result, 34 measurement items have been further anylysis. 4. As a result of principal component analysis on the 34 items, the four principal components have been found obtaines and inter-preted. The four principal components are 1) the thick of the neck, 2) the front neck-line on the waist basic pattern, basic pattern, 3) the shape of the neck surface development, and 4) the back neck-line on the waist basic pattern. 5. According to the graphic informations concerning these principal components, the meaning of these four principal components has been grasped on the visual. As a result, there is a large individual difference in the shape of neck.

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A Survey on Physical Health Status and Health Behavior Practice in Elderly People (일 지역사회 노인의 신체적 건강상태와 건강행위에 관한 연구)

  • Jang Eun-Hee
    • Journal of Korean Academy of Fundamentals of Nursing
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    • v.11 no.2
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    • pp.156-165
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    • 2004
  • Purpose: The purpose of this study was to Identify the physical health status and health behavior practice of elderly people in order to provide basic data for effective nursing interventions to promote health and quality of lift. Method: The participants for this study were 299 elderly persons in D city. Data were collected by interview with a questionnaire. Results: Average score for the physical health status of the participants was 3.98. There was a significant difference in average scores for physical health status for the variables age, sex, marital status, education level, religion, monthly income, source of living expense, perceived health status, alcohol use and type of household. The average score for the health behavior practice of the participants was 99.52, which means that elderly persons have good health behavior. There was a significant difference in average scores for health behavior practice for the variables age, sex, education level, perceived health status and type of household. Perceived health status, education level and alcohol use explained 50.6% of the variance for physical health status. Perceived health status and education level explained 27.4% of the variance for health behavior practice. Conclusion: To promote health behavior in elderly people, it is necessary to develop nursing interventions that take into consideration sociocultural traditions and demographic characteristics.

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A Study on Causes and Reactions of Intending Turnover of Staff Nurses Working in Hospitals (임상간호원의 이직에 대한 반응 및 이에 미치는 요인에 관한 조사연구)

  • 박세남
    • Journal of Korean Academy of Nursing
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    • v.6 no.2
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    • pp.10-17
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    • 1976
  • High turnover rate of nurses working in the hospitals is one of the serious problems in nursing society at the present time which can result in reducing the economic condition of hospital and providing low quality nursing care. This study was designed identify the causes and turnover rate of 248 clinical staff nurses in relation to their rewards and incentives in 6 hospitals located in Seoul from Oct.1 to Oct. 20, 1975. Questionnaires and analyzing items were formulated on the basic of Mallow's hierarchy of basic needs which were grouped in three categories such as safety and social and psychological rewards and incentives. The data were analyzed by means of mean, Chi square, Percentage and F- ratio. The following results were obtained: 1) There were 69% of respondents who have definitely decided or hoped to leave the hospital. 2) Those who are in the range of 26 to 30 years of age showed the strongest desire to leave the hospital. 3) There was no significant difference between those who want to leave the hospital and those who want to remain in relation to age, educational background, past experience and expecting period of clinical work. However, the married nurses tend to remain in the hospital than the single. 4) When the rewards scores in the three categories were calculated, psychological rewards were placed in the first rank by the mean score 14.09 ; and each of safety rewards and social rewards were 9.06 and 4.84. When the scores in three categories were compared it was found that there was significant difference. (F〈0.01).

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Correlation between Perception and Clinical Achievements of Nursing Students (간호학생의 지각과 실습성적 간의 상관 연구)

  • 한상숙
    • Journal of Korean Academy of Nursing
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    • v.6 no.2
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    • pp.25-31
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    • 1976
  • The present study was carried out for the period from July 14 through September 13, 1975 by means of questionnaire distributed to 300 nursing students whose extent of satisfaction is known to be relatively low compared with other students of the different course of study by applying the perceptual orientation scale based on the principle defining that the effectiveness in maintaining helpful relation with others in human society depends upon the perception possessed by individuals, The present study was intended to investigate the effectiveness of nursing profession for nursing students of the 4- year system and 3- year system nursing school and relationship between perceptual orientation and clinical achievements, thereby, attempting more efficient application of the perceptual orientation scale. The results obtained are summarized as follows : 1. There was observed no significant difference in perceptual orientation between the nursing students of the 4- year system and 3- year system nursing schools. 2. There was observed significant correlation between the perceptual orientation and clinical achievements of the nursing students. 3. In the comparison of the perceptual orientation Point and mean value by school significant difference was observed between schools other than B and C schools of the 4 - year system. 4. Marked correlation was observed between the perceptual orientation and clinical achievements of the students of 4 - year system nursing courses. 5. Low correlation was observed between the perceptual orientation and clinical achievements of the students of the 3- year system nursing school.

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Efficiency Effects of Mergers in the Korean Pharmaceutical Industry (국내 제약산업의 합병효과 분석)

  • Lee, Cheolhaeng;Cho, Keuntae
    • Journal of the Korean Operations Research and Management Science Society
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    • v.42 no.3
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    • pp.35-49
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    • 2017
  • The main objective of this paper is to empirically analyze the efficiency effects of mergers on Korean pharmaceutical firms and suggests managerial or policy implications for managers or policy makers. This study selects non-merging control firms close to the size of merging firms, as well as a set of merging firms, and measures the effects of mergers on efficiency three years before and after the merger using the non-parametric data envelopment analysis (DEA) method. To compare the differences of efficiency means among several groups, Wilcoxon rank sum test or Wilcoxon signed rank test is used. It showed that the long-term effects after the merger appeared partially. Furthermore, it was observed that there was no difference statistically in the efficiency between merging and non-merging firms. Also, there was no difference statistically in the efficiency between the pre- and post-merger periods. In conclusion, there was not any observed synergy effect through group (or affiliated) mergers between affiliates or related mergers for Korean pharmaceutical firms.