• Title/Summary/Keyword: Diethyl ether

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A Study of Cytotoxicity from Some Korean Edible Plants (수종 한국산 식용식물의 세포독성 연구)

  • 정하숙
    • Korean journal of food and cookery science
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    • v.15 no.2
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    • pp.108-113
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    • 1999
  • Natural products derived from not only medicinal but edible plants have been used as sources of folk remedies and other useful materials, like as appetizers, health supplements and food additives. A short-term in vitro biomarker assay was accompilshed to assess cytotoxic activity on the human lung and ovary adeno cancer cell lines based on sulforhodamine B (SRB) method. As a result, the EtOAc soluble fractions from Trichosanthes kirilowii Max. and Dioscorea japonica Thunb. showed potent cytotoxicity as a below 30% of growth ratio of cancer cell at a concentration of 40 $\mu\textrm{g}$/ml on lung and ovary adeno cancer cell lines, and lung cancer cell line, respectively. Cytotoxic activity present in plant extracts appear to be promising candidates as functional foods among Korean wild edible plants, and further studies are warranted.

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Antioxidant Activity of Various Solvent Extracts Obtained from A Maillard-type Browning Reaction Mixture (각종용매(各種溶媒)로부터 추출(抽出)한 Maillard형(型) 갈색화반응(褐色化反應) 생성물(生成物)의 산화억제작용(酸化抑制作用))

  • Won, Jong-Tai;Kim, Dong-Hoon
    • Korean Journal of Food Science and Technology
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    • v.12 no.4
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    • pp.235-241
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    • 1980
  • Equal portions of a Maillard-type browning mixture (0.2 M glucose+0.2 M glycine), heated at $100^{\circ}C$ for 12 hr, were extracted with the same amounts of eight solvents, respectively. The extracts were then dissolved in equal amounts of an edible soybean oil, and the resulting substrates and a portion of the soybean oil (Control) were stored in an incubator kept at $45.0{\pm}1.0^{\circ}C$ for three weeks. Peroxide values and TBA values of Control and the substrates were determined regularly during the storage period. The POVs of Control and the substrates containing acetone, benzene, chloroform, ethanol, diethyl ether, methanol, methylene chloride, and petroleum ether extracts after 12 days of storage were respectively $60.0{\pm}3.6$, $31.9{\pm}0.9$, $37.6{\pm}2.2$, $48.1{\pm}1.1$, $11.9{\pm}1.3$, $4.85{\pm}0.4$, $11.5{\pm}1.0$, $45.3{\pm}0.3$, and $43.3{\pm}4.2\;m.\;mole/kg\;oil$. The TBA values after 16 days of storage were respectively $0.28{\pm}0.02$, $0.20{\pm}0.01$, $0.21{\pm}0.01$, $0.26{\pm}0.03$, $0.16{\pm}0.02$, $0.28{\pm}0.02$, $0.17{\pm}0.01$, $0.33{\pm}0.05$, and $0.31{\pm}0.02$. The induction periods (arbitrarily taken as the time in hours for a substrate to reach a peroxide value of 30 m. mole/kg oil) of Control and the substrates were respectively 193, 280, 252, 220, 478, 229, 455, 217, and 214 hr. The antioxidant activity of each extract estimated on the basis of the length of the induction periods was, in decreasing order, as follows; ethanol>methanol>acetone>benzene>diethyl ether>chloroform, pertroleum ether, methylene chloride.

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A Study on Capillary Gas-Liquid Chromatographic Determination of Diosgenin in Costus Speciosus (모세관 기체-액체 크로마토그래피에 의한 Costus Speciosus 중 Diosgenin의 정량에 관한 연구)

  • Taek Jae Kim;Cha Kee Surk;Kim Young Sang
    • Journal of the Korean Chemical Society
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    • v.30 no.4
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    • pp.369-376
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    • 1986
  • Diosgenin in an Indonesian Costus speciosus was determined by capillary gas-liquid chromatography (GLC). The experimental conditions for the hydrolysis, extraction and acetylation of the diosgenin, and the determination by GLC were investigated. 0.20g of dried sample powder was refluxed in the solution of 3N HCI and xylene at 95∼100${\circ}C$ for 4 hours and the xylene layer was separated. The residue evaporated the xylene was refluxed in 20 : 80 acetic anhydride-pyridine for 30 minutes and the diosgenin acetate was extracted with diethyl ether. Dehydrated with anhydrous $Na_2SO_4$ and evaporated the ether, the residue was dissolved in 5.00ml of n-hexane and injected into GLC. Capillary column of SE-30 25m ${\times}$ 0. 33mm was installed in GLC and the column temperature was increased from 180${\circ}$ to 270${\circ}C$ at rate of 10${\circ}C$/min. The flow rate of carrier gas $N_2$ was 2ml/min and FID was used to detect. The analytical result of the diosgenin was 0.281% and relative standard deviation of 5 measures was 1.8%.

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Antioxidant Activity of Rubus crataegifolius Bge. Fruit Extracts (Rubus crataegifolius Bge. 열매 추출물의 항산화 활성)

  • Moon, Kyoung-Mi;Kim, Ji-Eun;Kim, Hae-Young;Lee, Jae-Seol;Son, Gi-Ae;Nam, Soo-Wan;Kim, Byung-Woo;Lee, Jong-Hwan
    • Journal of Life Science
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    • v.21 no.9
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    • pp.1214-1218
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    • 2011
  • We investigated the fruits of Rubus crataegifolius Bge, a plant which has been traditionally used in Korea in phytotherapy, to describe antioxidant materials from plant sources. R. crataegifolius fruits were extracted with methanol and further fractionated into n-hexane, diethyl ether, and ethyl acetate. The antioxidant activity of each fraction and the residue was assessed using a 1,1-diphenyl-2-picrylhydrazyl (DPPH), $H_2O_2$ radical scavenging method, and their cytotoxicity on human primary kerationcyte (HK) was determined by an MTS assay. The R. crataegifolius fruit methanol extract showed strong antioxidant activity (75.04%, 50%) compared with vitamin C (79.9%, 54.1%) by the DPPH, and $H_2O_2$ method, respectively. The measured activity from the subsequent extracts of the methanol extract were 20.3% for n-hexane fraction (HF), 68.8% for diethyl ether fraction (DF), 67.1% for ethyl acetate fraction (EF), and 67.1% for the residue fraction (RE) by DPPH and 2.2% for HF, 1.6% for DF, 10% for EF, and 50% for the RE by $H_2O_2$ assay. An oxidative stress model of HK was established under a suitable concentration (1 mM). The cell viability of the RE treated group increased and the percentage of apoptotic cells decreased at concentrations of 0.005-0.02% RE compared with the $H_2O_2$ treated group. Fruit extracts of the medicinal plant R. crataegifolius showed potent antioxidant activity and the ability to relieve cell damage from $H_2O_2$ induced injury to HK.

Studies on the Antimicrobial Activities of the Extractives from Magnolia (Magnolia kobus DC. var. borealis Sarg.) (목련(Magnolia kobus DC. var. borealis Sarg.) 추출성분의 항균성에 관한 연구)

  • Kim, Yun-Geun
    • Journal of the Korean Wood Science and Technology
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    • v.27 no.1
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    • pp.105-114
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    • 1999
  • Antimicrobial activities of the organosoluble extracts, seperated fractions and isolated lignans from the leaves tissue of Magnolia kobus DC. var. borealis Sarg. were investigated. The results are summarized as follows : 1. The inhibitory components against the spore growing test were concentrated on light petroleum ether and diethyl ether soluble fractions. The light petroleum ether solubles of the leaves tissue had terpenes compound, so, that they caused growing inhibition. These appearance showed high values of Rf on TLC bioautography and GC analyses with monoterpenes. 2. In the lignans, syringaresinol(X III), medioresinol(VI), phillygenin(VIII), kobusinol A(X) showed relatively high inhibitory effects in the spore growing test, and these are all showed structural characteristic of the phenolic hydroxyl group of guaiacyl and syringyl skelecton. 3. The light petroleum ether soluble fraction showed the strongest inhibitory effect against the antimicrobial activity in the seperated fractions. 4. The inhibitory effects of the lignans against the bacteria showed not so pronounced independantly, but the extracts and separated fractions contained with these lignans showed something synergism.

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An Experimental study on Analysis of Hydrocarbon of Exhaust gas Using Oxygenated Fuels by Gas Chromatography in Diesel Engine (디젤기관의 배기 배출물 중 가스 크로마토그래피를 이용한 탄화수소분석에 관한 실험적 연구)

  • Choi, S.H.;Oh, Y.T.
    • Journal of Power System Engineering
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    • v.4 no.3
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    • pp.12-18
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    • 2000
  • Recently, our world is faced with very serious and hard problems related to the air pollution due to the exhaust emissions of the diesel engine. So, lots of researchers have studied to reduce the exhaust emissions with various methods of diesel engine that influenced the environment strong. In this paper, the quantities of the low and high hydrocarbon among the exhaust emissions in diesel engine have been investigated by the quantitative analysis of the hydrocarbon $C_1{\sim}C_6$ using the gas chromatography. This study carried out by comparing the chromatogram with diesel fuel and three kinds of mixed fuels. One is the diesel fuel blended DGM(diethylene glycol dimethyl ether) 5%. Another is the diesel fuel blended DEE(diethyl ether) 25% and DMC(dimethyl carbonate) 10%. The results of this study show that the hydrocarbon $C_1{\sim}C_6$ among the exhaust emissions of the mixed fuels are exhausted lower than those of the diesel fuel at the all load and speed.

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Studies on the Separation and Identification of Acids in Izmir Tobacco Leaves (Izmir 잎담배 중 Acids 성분의 분리 및 확인에 관한 연구)

  • Lee, Un-Chul;Jang, Gi-Chul;Kim, Yong-Ok
    • Journal of the Korean Society of Tobacco Science
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    • v.16 no.2
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    • pp.172-180
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    • 1994
  • This study was carried out to separate and identify the acidic compounds in tobacco leaves. Izmir tobacco leaves was extracted with isopropyl ether and the extract was concentrated. The concentrate was extracted with 6% NaHCO3 aqueous solution. The aqueous extract was acidified with sulfuric acid, and extracted with diethyl ether. The acidic material was fractionated on silicic acid column using a benzene-methanol mixture with a stepwise increasing methanol concentration. The resulting fractions were esterified with diazomethane, and then identified by GC, GC/MS using SPB -5 fused silica capillary column. Most of acidic compounds in Izmir tobacco leaves were elected from fraction B which was benzene-methanol(98 : 2) mixture on silicic acid column chromatography. The identified acidic compounds of Izmir tobacco leaves were 18 saturated acids, 8 unsaturated acids, 5 dicarboxylic acids, 13 aromatic acids and 7 terpenoid acids. The major acidic compounds of lzmir tobacco leaves were 2- methylbutanoic, 3-methyl butanoic, 3- methylpentanoic, hexanoic, nonanedioic, phenylacetic, benzoic, 4- methoxybenzoic, 3, 5- dimethoxybenzoic, methoxycinnamic and 3, 4- dimethoxycinnamic acid. Key Words : Izmir tobacco, Acidic compounds, GC/MS.

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The Absorbance and Fluorescence of Chlorophyll-a in Organic Solution (I) (유기용매 중에서 Chlorophyll-a의 흡광 및 형광 (제1보))

  • Choong-Hwa Lee;Byong-Soo Kim;Jung-Hee Kang;Myon-Yong Park
    • Journal of the Korean Chemical Society
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    • v.26 no.4
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    • pp.218-223
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    • 1982
  • The absorbance and fluorescence yields of chl-a vs. concentration of n-prOH in diethyl ether, benzene and iso-octane were shown the characteristic point which chl-a structures are changed to monomer by the solvation of oligomer, and the spectral differences of fluorescence excitation between oligomer and monomer were identified by fluorimetry. All the maximum wavelength of absorbance, fluorescence excitation and fluorescence emission were shifted to longer wavelength. The ratios of soret/red band were depended on the band intensions and the polarities of solution in organic solvents mixed with n-prOH.

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Comparative Studies of Five Solvents for the Extraction of Polar Lipid in Corn Embryo (옥수수 배의 극성 지질의 추출을 위한 5종 용매의 비교연구)

  • 김덕진;전영민
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.20 no.6
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    • pp.590-595
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    • 1991
  • In order to investigate polar lipid ingredients and fat쇼 acid compositions in corn embryo, lipids were extracted with n-hexane (HX), pet. ether (PE), chloroform-methanol (2:1, v/v) (CM), dichoromethane-methanol (2:1, v/v DM) and hexane-diethyl ether (5:1, v/v HD). Of the glycolipid in polar lipids were separated by thin layer chormatogarphy (TLC), monoglycosyl diacyglycerol was most efficient with CM, HD, and monoglycosy sterol and monoglycosyl ceramide were similar to five solvents, but HX, PE and DM, HD were somewhat superior, respectively. Of the phospholipid, phosphatidyl inositol was most efficient with CM, DM, and phosphatidyl choline was similar to five solvents as well. Phosphatidyl serine was superior PE, HD, CM to HX, and phosphatidyl ethanolamine was inferior CM to HX. The major fatty acid in the glycolipid was linoleic acid, and it was most efficient with CM the same as plamitic acid, but oleic acid was superior in using HX, PE. The major fatty acids in the phospholipid were palmitic, heptadecanoic aicds, and they were superior in using HX and PE, respectively. Also oleic acid was most efficient with HX and CM, but HD was somewhat inferior.

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