• Asian-Australasian Journal of Animal Sciences
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• 제17권11호
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• pp.1562-1569
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• 2004

The objective of this experiment was to determine the effects of n-3 polyunsaturated fatty acids (n-3 PUFAs)-enriched diet supplemented with different levels of $\alpha$-tocopherol on the activities of hepatic lipogenic-related enzymes and the contents of liver and plasma lipid fractions in laying Tsaiya ducks. A total of 180 30-wk-old laying Tsaiya ducks, at the beginning of peak production, were allotted into 6 treatments with 3 replicates each. Ducks were fed one of the 6 experimental diets, containing 4% tallow (control), and 4% fish oil supplemented with graded levels of $\alpha$-tocopheryl acetate ($\alpha$-tocopherol) at 0, 100, 200, 300 and 400 mg/kg, respectively, for 6 wks. Feed and water were supplied ad libitum throughout the experimental period. The results indicated that the n-3 PUFAsenriched diet supplemented with different levels of $\alpha$-tocopherol did not affect (p>0.05) egg weight, feed intake, body weight change or liver and abdominal fat weights. Egg production, egg mass and feed efficiency significantly (p<0.05) improved as dietary $\alpha$-tocopherol levels increased. The activities of hepatic lipogenic-related enzymes including acetyl-CoA carboxylase (EC 6. 2. 1. 3; ACC), glucose-6-phosphate dehydrogenase (EC 1. 1. 1. 49; G-6-PDH), ATP-citrate cleavage enzyme (EC 4. 1. 3. 8; CCE), NADP-malate dehydrogenase (EC 1.1.1.40; NADP-MDH) and fatty acid synthetase (FAS) were higher (p<0.05) in birds fed with the tallow diet than in those fed with fish oil diets and increased with increasing dietary $\alpha$-tocopherol levels. None of the dietary treatments significantly affected the contents of triglyceride and total cholesterol in the liver, or total cholesterol, phospholipid and total lipid in the plasma. However, the contents of phospholipid and total lipid in the liver, and triglyceride in the plasma increased as dietary $\alpha$-tocopherol levels increased. Increasing dietary $\alpha$-tocopherol levels decreased the non-esterified fatty acid (NEFA) content in the plasma and trended to decrease the cholesterol contents in the egg yolk. The lipid metabolism of laying Tsaiya ducks was influenced not only by the dietary fat but also by the supplementation levels of $\alpha$-tocopherol.

• 한국가금학회지
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• 제33권2호
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• pp.133-139
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• 2006

본 시험은 육계 사료내 Biotite V의 첨가가 육계의 성장 능력, 영양소 소화율, 혈청 및 육내 콜레스테롤 함량에 미치는 영향을 조사하기 위하여 실시하였다. 총 480수의 2 일령된 Arbor Acre Broiler(male) 병아리를 공시하였고 사양시험은 5주간 실시하였다. 시험 설계는 육계 전기 사료로서 1) CON (basal diet), 2) BV200 (basal diet+200 mesh Biotite V 1.5%) 및 3) BV325 (basal diet+325 mesh Biotite V 1.5%) 를 첨가하여 제조하였다. 처리당 8반복, 반복당 20수씩 완전 임의 배치하였다. 개시시부터 1주까지의 증체량에서는 Biotite V를 첨가한 처리구가 대조구에 비하여 유의적인 증가를 보였다(P<0.05). 전체 시험기간 동안의 증체율, 사료 섭취량과 사료효율에 있어서는 Biotite V 를 첨가한 처리구간에 유의적인 차이를 보이지 않았다(P>0.05). 건물과 질소 소화율에서는 처리구간의 차이를 보이지 않았다(P>0.05). Biotite V 첨가구에서 복강내 지방 무게, 혈청과 육내 콜레스테롤 함량이 대조구에 비해 유의적인 차이는 없었다(P>0.05). 결론적으로, 육계 사료내 Biotite V의 첨가는 성장율, 영양소 소화율, 혈청 및 육내 콜레스테롤 함량에 영향을 미치지 않았다.

• 한국축산식품학회지
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• 제30권2호
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• pp.305-312
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• 2010

본 시험은 육계에 엄나무 가지와 잎을 급여하여 5주간 사육한 육계의 생산성과 계육의 일반성분, pH, 총페놀함량, 전자공여능, 콜레스테롤 함량 및 지방산 조성을 조사하였다. 실험구는 엄나무를 첨가 급여하지 않은 처리구를 대조구, 엄나무 가지 5% 급여구는 T1, 엄나무 잎 2% 급여구는 T2 그리고 엄나무 가지와 잎을 중탕하여 그 추출물을 급여한구를 T3 등 4개 처리구로 나누어 사양하였다. 육계의 사료요구율은 T1과 T3가 유의적으로 향상되었다. 계육의 일반성분은 엄나무 급여구에서 조지방 함량이 증가하는 경향이나, 일반성분은 유의성이 없었다. pH는 처리구간 유의성이 없었으며, T3에서 높은 경향이었다. 총 페놀 함량과 전자공여능은 엄나무 가지와 잎의 급여구에서 높았고, 엄나무 가지와 잎의 혼합 중탕구인 T3에서 그 함량이 가장 높았다(p<0.05). 계육의 총 콜레스테롤과 HDL-cholesterol은 엄나무 급여구에서 높았고, 특히 T3에서 가장 높았으며 LDL-cholesterol은 낮아지는 결과이었다(p<0.05). 지방산 조성은 엄나무를 급여함에 따라 지방산함량이 증가하였으며, 포화지방산은 감소하고, 불포화지방산이 증가하는 결과를 보이고 있으며, 특히 T3에서 이러한 경향은 뚜렷하였다. 결론적으로 엄나무 가지와 잎의 중 탕 처리구는(T3) 총콜레스테롤, HDL-cholesterol 및 불포화지방산을 증가시키고, LDL-cholesterol과 포화지방산을 감소시킴으로 cholesterol 및 지방산 조성을 개선할 가능성이 있는 것으로 판단된다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2003년도 추계학술대회
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• pp.118-118
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• 2003

The influence of dietary supplementation with dehydroepiandrosterone-sulphate (DHEA-S) at 0.6% was investigated in male Syrian golden hamsters initiated by treatments with azoxymethane(AOM), and dihydroxy-di-n-propyl nitrosamine (DHPN), timed after transfer from a choline-deficient to a normal diet.(omitted)

• 대한유전성대사질환학회지
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• 제22권1호
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• pp.21-27
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• 2022

Very long-chain acyl-coenzyme A dehydrogenase (VLCAD) deficiency (VLCADD) leads to a defective 𝛽-oxidation, specifically during prolonged fasting, infection, or exercise. Patients with VLCADD usually suffer from cardiomyopathy, hypoketotic hypoglycemia, hepatic dysfunction, exercise intolerance, muscle pain, and rhabdomyolysis, and sometimes succumb to sudden death. VLCADD is generally classified into three phenotypes: severe early-onset cardiac and multiorgan failure, hypoketotic hypoglycemia, and later-onset episodic myopathy. Diagnostic evaluation comprises acylcarnitine analysis, genetic analysis, and VLCAD activity assay. In the acylcarnitine analysis, the key metabolites are C14:1, C14:2, C14, and C12:1. A C14:1 level >1 mmol/L strongly suggests VLCADD. Various treatment recommendations are available for this condition. Dietary management includes decreasing fat content, increasing medium-chain triglyceride levels, and decreasing fasting periods. Supplementation with L-carnitine is controversial. Triheptanoin (a seven-carbon fatty acid triglyceride) treatment demonstrates improvement of cardiac functions. Bezafibrate may improve the quality of life of patients with VLCAD.

• 한국가금학회지
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• 제37권4호
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• pp.323-329
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• 2010

본 시험은 육계에 엄나무 잎 분말을 첨가량에 따라 급여하여 5주간 사육한 육계의 생산성과 계육의 일반성분, 도체 특성 및 혈액 성상을 조사하였다. 실험구는 엄나무 잎 분말을 첨가 급여하지 않은 처리구를 대조구, 엄나무 잎 분말 0.5% 급여구는 T1, 엄나무 잎 분말 1% 급여구는 T2, 그리고 엄나무 잎 분말 2.0% 급여구를 T3 등 4개 처리구로 나누어 사양하였다. 육계의 생산성은 처리구간 유의적인 변화는 없었고, 폐사율은 엄나무 잎 분말 급여구에서 낮아졌으며, 엄나무 잎 급여량에 의한 차이는 없었다. 계육의 일반성분은 엄나무 잎 분말 급여구에서 조지방 함량이 증가하였고(T3), 수분, 조단백질 및 조회분은 유의성이 없었다. 도체 특성 중도체중, 도체율, 간의 무게 및 비장 무게는 처리구간에 유의성이 없었고, 복강지방은 엄나무 잎 분말의 급여량이 늘어남에 따라 증가하는 경향으로 T3에서 유의적으로 무거웠다(P<0.05). 육계의 혈액 성상 중 HDL-cholesterol과 glucose는 엄나무 급여구에서 높았고, 총 콜레스테롤과 LDL-cholesterol 및 triglyceride는 낮아지는 결과이었다(P<0.05). 이러한 경향은 엄나무 잎 분말 1% 급여구부터 나타났다. 결론적으로 엄나무 잎 분말을 1% 이상 급여하면(T2) 육계의 폐사율이 낮아지고, 총콜레스테롤, LDL-cholesterol이감소하며, HDL-cholesterol이 증가함으로 혈액 조성을 개선할 가능성이 있는 것으로 판단된다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권9호
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• pp.1246-1254
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• 2004

Two separate trials were designed to determine effects of dietary level of whole flaxseed (WFS) on fatty acid composition of serum, and subcutaneous, perirenal, intermuscular, and intramuscular adipose tissues of Korean Hanwoo cattle. Twentyone bulls (trial 1) and 15 cows (trial 2) were assigned to diets containing 0, 10 or 15% WFS. Relative treatment effects were similar between bulls and cows. The proportion of C18:3 in serum and to a lesser extent in adipose tissues were increased by dietary inclusion of WFS, reflecting supplemented lipid composition of WFS that escaped ruminal biohydrogenation. Animals fed WFS had a lower proportion of saturated fatty acids in serum and adipose tissues than animals fed diets without WFS, while the opposite trend was observed in unsaturated fatty acids with little differences between two WFS groups. WFS-fed animals had higher proportions of C18:1, 18:2, 18:3, 20:3, and 22:3 and lower proportions of C12:0, 14:0, 16:0 and 18:0 in intramuscular fat than animals fed diets without WFS. Furthermore, feeding WFS increased proportions of both $\omega$-3 and $\omega$-6 fatty acids but decreased the ratio of $\omega$-6/$\omega$-3 substantially. In conclusion, feeding WFS can be an effective method of increasing absorption of unsaturated fatty acids, and subsequent deposition in adipose tissues.

• Asian-Australasian Journal of Animal Sciences
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• 제7권1호
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• pp.103-112
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• 1994

This study was a sequential experiment consisting if feeding trial and in vitro culture studies. Feeding was conducted by $2{\times}2{\times}2$ factorial design with two cimaterol levels (0, 0.25 mg/kg), two energy levels (3,200, 2,900 ME kcal/kg) and two sexes. In starting period (0-21 days) broilers were fed diets containing two energy level without dietary supplementation of cimaterol. During finishing period (21-42 days) cimaterol groups were fed cimaterol supplemented diets. In vitro cultures were carried out to study the cellular metabolism of protein and fat in liver and adipose tissues prepared from chicks used in feeding trials. Body weight gain was significantly improved by the administration of cimaterol to experimental diets by 2.4% (p < 0.05). Feed intake was reduced by cimaterol administration at the high energy level, but this trend was reversed at low energy level. Feed efficiency was improved by cimaterol administration and at high energy level the difference (5.7%) was significant(p < 0.05). The administration of cimaterol had no effects on percentage of abdominal fat content, giblet and neck. There was little difference in carcass yield between control and cimaterol treated group. The administration of cimaterol had no effects on nutrient metabolizability or carcass composition. The results of in vitro studies with liver tissues showed that cimaterol increased the lipolytic activities (p < 0.05) and decreased lipogenic activities (p < 0.05). In in vitro studies with acinar cell of liver tissues. cimaterol increased the amount of retained protein and decreased secreted protein at high energy level. but the trend was opposite at low energy level.

• Asian-Australasian Journal of Animal Sciences
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• 제17권1호
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• pp.102-108
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• 2004

The present experiment was conducted to assess the efficacy of methionine hydroxy analogue free fatty acid (MHAFA) in comparison to DL-methionine (DL-Met) utilizing day-old commercial broiler chicks (n=300). The chicks were randomly distributed into 30 groups of 10 chicks each. Three dietary treatments, viz. D1-maize-soybean meal based basal diet (Control), D2- control diet supplemented with DL- methionine to meet its requirements and D3-control supplemented with MHA-FA @ 1.54 times of DL-methionine used in D2, were formulated. Each dietary treatment was offered to 10 replicated groups of chicks following completely random design (CRD). The chicks fed on supplemental DL-Met had significantly higher (p<0.01) gain in body weight, followed by MHA-FA group and control during 0-3 weeks of age. During overall growth period (0-6 weeks), chicks in DL-Met and MHA-FA groups grew better (p<0.01) than those in control. Feed conversion ratio (FCR) improved (p<0.01) on supplementation of either DL- Met or MHA-FA in the basal (Control) diet during 0-3 weeks of age. The FCR for overall period, however, did not differ statistically (p>0.05) amongst the treatments. The eviscerated yields emanated from diets with DL-Met or MHA-FA were higher (p<0.01) than that in Control. Abdominal fat pad was also more (p<0.01) in broilers fed control diet than in DL-Met or MHA-FA supplemented group. Breast yield was higher (p<0.05) in MHA-FA fed broilers than those fed DL-methionine supplemented or un-supplemented diets. The efficacy of MHA-FA in comparison to DL-Met for growth was 62.11, 64.82 and 63.88% and for feed efficiency was 62.98, 67.73 and 64.01% at 0-3, 3-6 and 0-6 weeks of age, respectively, while it was 65.85, 71.40 and 67.49% for eviscerated yield, abdominal fat pad reduction and breast yield at 6 weeks of age, respectively.

• Journal of Nutrition and Health
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• 제35권4호
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• pp.421-430
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• 2002

사철쑥 (Artemisia capitlaris Thunberg)으로 성인병의 예방목적으로 흰쥐 (Sprague-Dawley rats)의 혈액 및 간지질 대사에 미치는 영향에 대한 효과를 알아보았다. 1) 사료이용효율은 L, LM, LP군에서 높았다 (p < 0.01). 혈청의 콜레스테롤 농도, 동 맥 경화지수 (p <0.01), 중성지질 (p < 0.01)은 L, LM, LP군에서 높았지만, 사철쑥 분말 첨가에 의해 HDL-콜레스테롤과 HTR를 증가시켰다 (p < 0.01). 2) 혈청 및 간 조직의 과산화지질은 C군에 비하여 L군이 유의적으로 많았으며, 사철쑥 첨가시 감소하였다 (p < 0.05). 3) 간 조직의 지방침착은 L군에서 지방양성구의 크기와 분포가 두드러지게 높았고. LM군, LP길군에서는 감소하였다. 이상의 결과는 사철쑥은 혈액 및 간의 지질조성 및 지질 대사에 바람직한 효과를 갖는 것으로 보인다. 이는 사철쑥이 성인병예방에 유용한 기능성 식품으로서 가치가 있을 것으로 사료된다.