• Mycobiology
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• 제45권2호
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• pp.73-83
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• 2017

The ability of dead cells of endophytic Drechslera hawaiiensis of Morus alba L. grown in heavy metals habitats for bioremoval of cadmium ($Cd^{2+}$), copper ($Cu^{2+}$), and lead ($Pb^{2+}$) in aqueous solution was evaluated under different conditions. Whereas the highest extent of $Cd^{2+}$ and $Cu^{2+}$ removal and uptake occurred at pH 8 as well as $Pb^{2+}$ occurred at neutral pH (6-7) after equilibrium time 10 min. Initial concentration 30 mg/L of $Cd^{+2}$ for 10 min contact time and 50 to 90 mg/L of $Pb^{2+}$ and $Cu^{2+}$ supported the highest biosorption after optimal contact time of 30 min achieved with biomass dose equal to 5 mg of dried died biomass of D. hawaiiensis. The maximum removal of $Cd^{2+}$, $Cu^{2+}$, and $Pb^{2+}$ equal to 100%, 100%, and 99.6% with uptake capacity estimated to be 0.28, 2.33, and 9.63 mg/g from real industrial wastewater, respectively were achieved within 3 hr contact time at pH 7.0, 7.0, and 6.0, respectively by using the dead biomass of D. hawaiiensis compared to 94.7%, 98%, and 99.26% removal with uptake equal to 0.264, 2.3, and 9.58 mg/g of $Cd^{2+}$, $Cu^{2+}$, and $Pb^{2+}$, respectively with the living cells of the strain under the same conditions. The biosorbent was analyzed by Fourier Transformer Infrared Spectroscopy (FT-IR) analysis to identify the various functional groups contributing in the sorption process. From FT-IR spectra analysis, hydroxyl and amides were the major functional groups contributed in biosorption process. It was concluded that endophytic D. hawaiiensis biomass can be used potentially as biosorbent for removing $Cd^{2+}$, $Cu^{2+}$, and $Pb^{2+}$ in aqueous solutions.

• 임산에너지
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• 제22권1호
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• pp.49-57
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• 2003

경상남도 진해지역 30년생 편백 조림지를 대상으로 현존량을 추정하기 위하여 Urich II법에 의하여 수관급에 따른 부위별 현존량을 측정하여 생산구조를 해석하고 시료채취의 효율성을 높이기 위한 방법을 탐색하였다. 지상부 총 물질생산량은 137.3 ton/ha을 보유하고 있었으며, 이들 임목의 부위별 구성비는 줄기 71.8%, 가지 15.4%, 잎 12.8%로 나타났다. 임분에서 엽생산량은 수고 9.2∼11.2m에서 최대치를 보였으며, 현존량의 구성비는 우세목과 준우세목에서는 줄기>가지>잎, 열세목에서는 줄기>잎>가지 순으로 나타났다. 부위별 건조비는 줄기 47.3∼49.2%, 가지 48.8∼52.9%, 잎 39.2∼40.8%, 고사지 0.78%∼0.89%의 범위를 보였다. 표준목 전건비중(basic wood density)은 0.49∼0.53으로 나타났으나 수관급에 따른 표준목간 유의차는 나타나지 않았다.

• 임산에너지
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• 제19권1호
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• pp.7-12
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• 2000

본 실험은 광도가 곰솔(Pinus thunbergii Parl.) 유묘의 생장과 물질생산에 어떠한 영향을 미치는 가를 알고자 자연전광이 100, 50, 30, 10% 및 2%로 조절된 야외포지에서 1997년 5월부터 1998년 10월까지 실시되었다. 곰솔의 수고생장 및 근원경생장은 1,2차년을 통하여 자연전광에서 가장 높악으며, 50%에서의 수고생장은 1,2차년도 각각 대조구의 96.4, 88.9%로, 근원경생장은 각각 94.1 77.6%로 조상되엇다, 30%이하로 상대광도가 낮아질수록 수고 및 근원경생장이 급격히 감소 되었으며, 근원경생장의 감소율이 수고생장의 감소율보다 높게 나타났다. 2차년도의 2%에서는 시험 유묘 모두가 고사하였다. 물질생산량도 광도가 낮아짐에 따라 감소되었으며, 1, 2차년도 모두 뿌리의 감소율이 잎, 줄기보다 높았고, 1차년도에는 줄기의 감소율이, 2차년도에는 잎이 감소율이 가장 낮았다. T/R율은 1차년도에는 50%에서,3.55, 2차년도에는 10%에서 4.88로 가장 높았다.

• ALGAE
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• 제36권4호
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• pp.231-240
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• 2021

Saccharina sculpera is highly valued for human consumption and value-added products. However, natural resources of this kelp have decreased sharply and it is in danger of extinction. Resources recovery through cultivation is being trialed to enable the sustainable use of this species. In this study, the temperature range for survival and optimal growth of juvenile S. sculpera was identified and applied to field cultivation. This study investigated the survival and growth of juvenile S. sculpera under six temperatures (i.e., 5, 10, 15, 16, 18, and 20℃) and two light intensities (i.e., 20 and 40 µmol photons m^-2 s^-1) in an indoor culture experiment. In these experiments, the blade length decreased at 16℃ under the both light intensities. The thalli died at 20℃ and 20 µmol photons m^-2 s^-1, and at 18-20℃ and 40 µmol photons m^-2 s^-1. During the field cultivation, early growth of S. sculpera was highest at the 5 m depth and growth decreased as the water depth increased. When the initial rearing depth was maintained without adjustment throughout the cultivation period (from December to October), all the cultivated S. sculpera plants died during August and September. However, S. sculpera plants lowered from 5 to 15 m and grew to 90.8 ± 13.1 cm in July. The seawater temperature at 15 m depth was similar to the upper level of thermal tolerance demonstrated by juvenile S. sculpera in the indoor culture experiments (16℃ or lower). The plants were subsequently lowered to 25 m depth in August, which eventually led to their maturation in October. The present study confirmed that improved growth rates and a delay in biomass loss can be achieved by adjusting the depth at which the seaweeds are grown during the cultivation period. These results will contribute to the establishment of sustainable cultivation systems for S. sculpera.

• 한국환경과학회지
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• 제9권3호
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• pp.267-273
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• 2000

In order to understand the impact for decomposition of blue-green algae Microcystis on water quality, the algae were cultivated with collection of natural population during approximately one month, when water-bloom of Microcystis dominated at August 31, 1999 in the lower part of the Okchon Stream. The enrichment of inorganic NㆍP nutrients didn't in algal assay and the effect of Microcystis on water duality was assessed from the variation of nutrients by algal senescence. Microcystis population seemed to play a temporary role of sink for nutrients in the water body. Initial algal density of Microcystis was 2.3×10/sup 6/ cells/㎖. When Microcystis population died out under light condition, algal NㆍP nutrients between 9∼12 days affected to increase of biomass after reuse by other algal growth as soon as release to the ambient water. However, cellular nutrients under dark condition were almost moved into the water during algal cultivation. NH₄, NO₃ and SRP concentration were highly increased with 160, 17 and 79 folds, respectively relative to the early. As a result, the senescence of Microcystis population seemed to be an important biological factor in which cause more eutrophy and increase of explosive algal development by a lot of nutrients transfer to water body. There are significantly observed an effort of reduce for production of inner organic matters such a phytoplankton as well as load pollutants from watershed in side of the water quality management of reservoir.

• Asian-Australasian Journal of Animal Sciences
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• 제19권4호
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• pp.521-525
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• 2006

The present study was undertaken to evaluate the effect of flushing of ewes with concentrate pellets just before the mating season on their nutrient utilization and reproductive performance on farms. Forty-eight Malpura ewes, 1-5 years old were randomly divided into 2 groups of 24 each (G1and G2). Ewes in both the groups were grazed on natural rangeland from 07.00 to 18.00 hr followed by night shelter in animal shed. G1 ewes were maintained on sole grazing while G2 ewes, in addition to grazing, received concentrate pellets at the rate of 1.5% of their body weight. The mean biomass yield of the community rangeland was 0.46 ton DM/hectare. The intakes of DM (g/kg $W^{0.75}$), DCP (g/kg $W^{0.75}$) and ME (MJ/kg $W^{0.75}$) were higher (p<0.01) in G2 as compared to that of G1 being 86.5, 10.2 and 1.15 and 57.5, 4.7 and 0.75, respectively. The digestibility of DM, OM, CP, NDF and hemicellulose were also higher (p<0.01) in G2 as compared to that of G1 being 57.2, 76.7, 78.9, 51.9 and 81.6 and 50.8, 68.7, 68.4, 45.4 and 74.4, respectively. The conception rate was higher (79.2%) in flushed ewes as compared to that of non-flushed (66.7%). Five of the pregnant ewes died and another 5 aborted in G1 while in G2, 5 ewes aborted with no mortality. The lambing was higher (73.7%) in G2 than that in G1 (37.5%). The birth weight of lambs was higher (p<0.05) in G2 (3.47 kg) than that in G1 (2.95 kg). Further, the birth weight of male lambs was higher (3.28) than that of female lambs (3.14). It is concluded that the biomass yield of the community rangeland in semi-arid region of India is low and insufficient to meet the nutrient requirement of ewes prior to mating season. However, concentrate supplementation at the rate of 1.5% of body weight to ewes during this critical stage enhanced their plane of nutrition, reproductive performance, body condition and birth weights of lambs.

• Applied Biological Chemistry
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• 제40권3호
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• pp.243-248
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• 1997

미나리 (O. javanica)는 오염된 하수 등에서도 매우 잘 자라는 식물체이다. 이런 미나리를 phytoremediation에 응용하기 위하여 $50\;{\mu}M$ 카드늄을 처리하여 카드늄에 대한 저항성 및 식물체에 축적된 카드늄을 정량하고 생리적, 생화학적 변화를 조사하였다. 카드늄을 처리하고 3주 뒤에 미나리는 거의 성장저해를 받지 않았으나 대조구로 사용한 담배 (N. tabacum Xanthi)는 75% 성장저해를 받았다. 미나리는 뿌리, 줄기, 잎에 건중량 1 그램당 각각 2.1, 7.3, $113\;{\mu}moles$의 카드늄을 축적하였다. 카드늄을 처리한 미나리의 경우 전체 thiol 잔기의 농도가 카드늄을 처리하지 않은 미나리에 비해 뿌리, 줄기, 잎에서 각각 0.5, 1,7배 증가하였으나 글루타치온 농도의 경우는 대조구에 비해 각각 57%, 30%, 53% 까지 감소하였다. 이를 HPLC로 조사해본 결과 증가된 thiol의 함량은 새로운 thiol 잔기를 지닌 물질의 생성으로 기인된 것이고, retention time을 기초로 이는 phytochelatin인 것으로 보여진다.

• 한국환경복원기술학회지
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• 제14권5호
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• pp.53-67
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• 2011

The major objective of this study was to quantify the effects of substrate depth and substrate composition on the development of sedum etc., in a sloped rooftop (6 : 12 pitch) environment during a 4-year period. The experiment was conducted from 2006 October to 2010 December under several conditions without soil erosion control : two substrate depth (5cm, 10cm), four substrate composition (A5N3C2, A3N3C4, A6C4, G5L3C2: A: artificial lightweight soil, N : natural soil, G : granite decomposed soil, C : leave composite, L : loess), four sloped roof direction ($E40^{\circ}W$, $W40^{\circ}N$, $S40^{\circ}W$, $N40^{\circ}E$). In this experiment 4 sedum etc., were used: Sedum sarmentosum, Sedum kamtschaticum, Sedum rupestre, Sedum telephium, flowering herbs (mixed seed : Taraxacum platycarpum, Lotus corniculatus, Aster yomena, Aster koraiensis), western grasses (mixed seed : Tall fescue, Creeping redfescue, Bermuda grass, Perennial ryegrass). The establishment factor had two levels : succulent shoot establishment (sedum), seeding (flowering herbs, western grasses). 1. Enkamat, as it bring about top soil exfoliation, was unsuitable material for soil erosion control. 2. Sedum species exhibited greater growth at a substrate depth of 10cm relative to 5cm. All flowering herbs and western grasses established only at a substrate depth of 5cm were died. A substrate depth of 5cm was not suited in sloped rooftop greening without maintenance. If additional soil erosion control will be supplemented, a substrate depth of 10cm in sloped rooftop greening without maintenance was considered suitable. 3. For all substrate depth and composition, the most abundant species was Sedum kamtschaticum. The percentage of surviving Sedum kamtschaticum was 73.4% at a substrate depth of 10cm in autumn 2007 one year after the roof vegetation had been established. But the percentage of surviving other sedum were 33.3%~51.9%, therefor mulching for soil erosion control was essential after rooftop establishment in extensive sloped roof greening was proved. To raise the ratio of plant survival, complete establishment of plant root at substrate was considered essential before rooftop establishment. 4. There was a significant interaction between biomass and substrate moisture content. There were also a significant difference of substrate moisture and erosion among substrate composition. The moisture content of A6C4 was highest, the resistance to erosion of A5N3C2 was highest among substrate composition. The biomass of plants were not significantly higher in A5N3C2 and A6C4 relative to A3N3C4 and G5L3C2, For substrate moisture and erosion resistance, A5N3C2 and A6C4 were considered suitable in sloped rooftop greening without maintenance. 5. There were significant difference among roof slope direction on the substrate moisture. Especially, the substrate moisture content of $S40^{\circ}W$ was lower relative to that of $N40^{\circ}E$, that guessed by solar radiation and erosion.

• The Korean Journal of Ecology
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• 제9권1호
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• pp.59-71
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• 1986

Net production, dead material increments were measured, and annual respiration loss was simulated through a year to determine the gross production at the Phragmites longivalvis, Carex scabrifolia and Zoysia sinica stand on Okryudeung, a sand bar of the Nagdong river estuary. The maximum live biomass for above-ground organs of the three stands occured in October, i.e., 1, 985, 744 and 1, 013g/m2, and belowground net productions were estimated to be 650, 440 and 412g/m2, respectively. Materials died or shedding from live aboveground organs during the growth season were estimated to be 167, 81 and 0 g/$m^2$. From the results of simulation, annual variation of respiratiion was primarily dependent on the annual variation of temperature through a year. For annual respiration loss in three stands, 21.893, 6.147 and 5.036kg $CO_2/m^2$ were calculated, respectively. Corresponding gross productions were 72, 203, 22, 109 and 19, 909kcal/$m^2$. Respiration of belowground organs corresponded to 65%, 66% and 37% of the total plant respiration, and annual respiration loss accounted for 85%, 78% and 71% of the annual gross production. In view of efficiency of solar energy utilization, 5.8%, 1.8% and 1.6% of incident light energy were converted to gross production of plants during a year. With incident light energy during the growth season from April to September, energy utilizations for net production were estimated to be 1.2%, 0.4% and 0.6% at the three stands.

• 유기물자원화
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• 제23권2호
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• pp.67-72
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• 2015

본 연구는 음식폐기물 건조사료를 곤충의 먹이(사료)로 공급하여 대량 증식을 위한 특성을 연구하였다. 특히 아메리카동애등에(Hermetia illucens)의 경우 음식폐기물 건조사료 함량에 따른 유충 증식량을 평가한 결과, 발육 초기에는 호프박과 일반사료를 음식폐기물 건조사료와 함께 혼합하여 공급한 경우 증체량이 증가하였으나, 발육이 서서히 진행되면서 종령시기에는 음식폐기물 건조사료 100%와 혼합사료간 유충의 중량에 큰 차이가 없었다. 집파리 유충은 음식폐기물 건조사료를 먹이로 100% 공급할 경우 발육이 부진하여 비정상적으로 용화되었으며, 정상적인 사육을 위해서는 음식폐기물 건조사료 함량을 20~30%로 낮게 공급하는 것이 효율적이다. 아메리카왕거저리의 유충은 사료의 종류에 크게 영향을 받지 않고 발육하는 것으로 나타났으며, 음식폐기물 건조사료에서 사망하는 개체가 조사되지 않아 먹이(사료)로 공급해도 생육을 저해하지 않았다.