• 한국환경복원기술학회지
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• 제12권5호
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• pp.1-12
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• 2009

This research was initiated to investigate the revegetation characteristics under the seeding amount treatments of cool-season turfgrasses on the disturbed slope. 4 different seeding amounts of cool-season turfgrasses (0.0 $g/m^2$, 1.5 $g/m^2$, 3.0 $g/m^2$, 6.0 $g/m^2$) with the same seeding amounts of native woody (Rhus chinensis, Albizzia julibrissin) and herbaceous plants (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena) were treated with 3 replications on the experimental disturbed slope. Data such as soil hardness (mm), moisture content (%), surface coverage rate (%), the number of each germinating plant and plant height were analyzed. There were no statistic differences observed in the soil hardness and the soil moisture content while a significant difference was observed in the surface coverage rates. The surface coverage rates were higher in 3.0 $g/m^2$ and 6.0 $g/m^2$ plots compared to the rest two plots of cool-season turfgrasses. However, the emergence of native woody and herbaceous plants was relatively low in 3.0 $g/m^2$ and 6.0 $g/m^2$ plots of high seeding amounts of cool-season turfgrasses. This result indicated that high seeding amounts of cool-season turfgrasses could decrease the emergence of native woody and herbaceous plants. We concluded that 1.5 g~3.0 $g/m^2$ seeding amounts of cool-season turfgrasses was optimum level for the balanced emergence of cool-season turfgrasses, native woody and herbaceous plants in the viewpoint of the ecological disturbed slope revegetation.

• Journal of Plant Biotechnology
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• 제37권1호
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• pp.89-95
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• 2010

Dihydroflavonol 4-reductase(DFR)는 flavonoid 생합성 경로의 가장 중심부에 작용하는 효소로 2R,3R-trans-dihydroflavonols로부터 leucoanthocyanidins 으로의 변환을 촉매한다. 본 연구에서는 색소유전자의 전이를 통하여 새로운 색소발현체계를 가진 품종을 육종하기 위한 기초연구로 안개초 (Gypsophila paniculata L.)의 꽃봉오리로부터 cDNAlibrary를 합성하였고 카네이션의 DFR 유전자를 probe로 사용하여 anthocyanin 합성경로의 중요 효소의 하나인 DFR 유전자를 분리하였다. 염기서열분석을 수행하여 분리유전자의 크기가 1279 bp이며 이 중 coding region은 1063 bp임을 확인하였다. 이미 밝혀진 다른 식물체의 DFR 유전자와 서로 염기서열의 일치성을 비교해 본 결과 Cheddar pink, 카네이션, 양배추, 개나리, 페튜니아, cup flower, 장미, 과꽃 및 거베라에서 각각 62% 이상을 나타내었다. 분리유전자의 발현을 확인하기 위하여 Northern blot 분석 및 인위적으로 기내에서의 transcription과 translation을 수행하였고, 분리한 유전자의 효소활성을 측정해 본 결과 leucopelargonidin의 작은 peak를 확인하였다. Southern blot 분석 결과 안개초의 DFR 유전자는 다른 대부분의 식물체와 유사하게 한 개가 존재함을 확인하였다.

• 한국환경복원기술학회지
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• 제4권3호
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• pp.84-95
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• 2001

This study was conducted to suggest the ecological restoration methods for the decomposed granite roadside cutslopes by Natural Topsoil Restoration Methods(NTRM). Four woody species, 3 shrub species, 4 native grasses, 8 wild flowers and 2 cool-season grasses were used. The experiment was conducted with the purpose of developing ecological restoration techniques by achieving following objectives for restoration by using native woody plants, shrubs, native plants and wild flowers; (1) prevention or reduction of wind and water erosion, (2) provision of food and cover for variety of animal species, (3) improvement of the visual or aesthetic quality of disturbed sites. The main results were summarized as follows. 1. Restoring naturalness to human-altered landscapes are important to protecting the worlds biodiversity. In this study, to restore or create an ecological community use as full a complement of native species as possible. Ecological restoration means returning a specific sites to its predisturbance condition. 2. Some species which are introduced first may inhibit the germination and growth of slower-growing species, or they may prevent the growth of other species whose propagules arrive later. In this study, cool season grasses was used small amount than native plants and woody species, because and Indigofera pseudo-tintoria grows well. Tall fescue and perennial ryegrass shows quick germination, but they could not inhibit the other species growing relatively slow. Pinus thunbergii used at Gonam near the ocean was suppressed by the other plants, so it can be concluded that using container seedlings is effective than seeding. 4. Ailanthus altissima, Albizzia julibrissin, Rhus chinensis Lespedeza cyrtobotrya, and Indigofera pseudo-tinctoria were germinated. In wild flowers, Coreopsis lanceolata, Dianthus sinensis, Coreopsis tinctoria, and Lotus corniculatus var. japonicus grew well and show enough plant height which will not suppressed by shrub and woody plants. After one year, native grasses such as Arundinella hirta, Miscanthus sinensis var. purpurascens and Imperata cylindrica var. koenigii cover the slopes well. 5. 25 herbaceous plants such as Trifolium repens, Arthraxon hispidus, Barbarea orthocerae, Potentilla fragarioides, Lepidium apetalum, Chenopodium album var. centrorubrum, Glycine soja, Rubus crataegifolius and 4 woody species such as Lespedeza maximowiczii, Robinia pseudoacacia, Symplocos Chinensis, Quercus acutissma were invaded. 6. Ecological restoration works by Natural Topsoil Restoration Methods for revegetation of the decomposed granite roadside cutslopes were successfully accomplished. It prevents surface water erosion and shows diverse vegetation structure. It will be successful to restore decomposed granite cutslopes.

• 한국초지조사료학회지
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• 제28권2호
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• pp.89-98
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• 2008

본 연구는 야생화초지 조성을 위한 기초연구로 국내산 야생화초지와 외국산 야생화초지의 2처리를 두어 시험하였다. 공시초종은 기본초종으로 잔디형 목초로 Kentucky bluegrass (Midnight) 를 공시하였고, 국내산 야생화는 패랭이꽃 (Dianthus sinensis L.), 별노랑이 (Lotus corniculatus var. Japonicus Regel), 꼬리풀 (Veronica linariaefolia pallas), 별개미취 (Aster koraiensis Nakai), 부처꽃 (Lythrum anceps Makino), 톱풀 (Achillea sibirica Ledeb), 붓꽃 (Iris nertschinskia Lodd) 및 원추 천인국 (Rudbeckia bicolor Nutt.) 등 8종을 공시하였고, 외국산 야생화는 서양말냉이 (Iberis umbellata), 기생초(Coreopsis tinctoria Nutt), 물망초(Myosotis sylvatica Hoffmann), Baby blue eyes (Nemophila menziesii), 참 제비꽃 (Delphinium ajacis), 아프리칸 데이지 (Dimorphotheca aurantiaca), 왜성 수레국화 (Centaurea cyanus), Dwarf godetia (Clarkia amoena), 왜성 기생초 (Coreopsis tinctoria, dwarf), 왜성 적 기생초(Coreopsis tinctoria, red), Spurred snapdragon (Linaria maroccana), 끈끈이대나물(Silene ameria), 러시안 꽃 무우(Cheiranthus allionii), 캘리포니아 양귀비(Eschscholzia californica Cham), 아이스랜드 양귀비 (Papaver nudicaule), 왜성 제비꽃 (Viola cornuta), Bellflower (Campanula carpatica), Sweet alyssum (Lobularia maritima), 흰 점나도나물 (Cerastium biebersteinii), 큰 달맞이꽃 (Oenothera lamarckiana Ser.), 큰 금계국(Coreopsis lanceolata L.), 각시 패랭이(Dianthus deltoids L.), Baby blue eyes (Nemophila menziesii) 및 참 제비꽃 (Delphinium ajacis) 등 24종을 공시하였다. 시험은 충남대학교 농과대학 초지시험포장에서 2006년 9월부터 2007년 12월까지 수행하였으며, 얻어진 시험결과는 다음과 같다. 야생화초지의 꽃 색깔은 외국산 야생화초지가 국내산 야생화초지보다 다양하였으나 개화가 봄철에 편중되는 등 계절분포 및 영속성이 문제시되었다. 국내산 야생화초지는 꽃 색깔은 다양하지 못하였으나 계절분포 및 영속성이 상대적으로 양호한 것으로 나타났다. 그러나 다년간 식생을 유지하기 위한 야생화 초지의 조성은 기본초종인 잔디형 목초는 물론이고 야생화초종을 지역별로 초기생육 및 개화특성 등을 검토하여 초종을 다양화하는 것이 중요하다고 하겠다. 더욱이 야생화초지를 추파하여 조성할 경우, 외국산 야생화초지는 월동전에는 다양한 식생을 유지하였으나 월동 후에는 내한성의 문제로 식생이 단순화되는 양상을 보였다. 국내산 야생화초지는 내한성에는 문제가 없었으나 식생이 다양화하지 못하였다. 따라서 야생화초지를 다년간 유지할 목적이라면 다년생의 야생화를 중심으로 하되 여기에 단년생을 혼합하여 야생화 초종을 다양화하는 것이 좋을 것으로 사료된다.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.61-71
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• 2016

The purpose of the research is to identify the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria as leguminous woody plants for the cut-slope revegetation. To investigate the coverage ratio and appearance frequency, we divided Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria into five treatment groups with various quantities of $0.0g/m^2$, $1.0g/m^2$, $2.4g/m^2$, $3.8g/m^2$, and $5.2g/m^2$. For each treatment group, we mixed the identical quantities of herbaceous flowers (Lotus corniculatus var. japonicus, Dianthus sinensis, Aster yomena and Pennisetum alopecuroides) seeds and cool-season turfgrasses (Festuca arundinacea and Poa pratensis) seeds. In this result, as the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria increased in the spray, the coverage ratio for leguminous woody plants appeared to increase whereas the coverage ratio decreased in herbaceous flowers and cool-season turfgrasses. However, when the seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria exceeded $3.8g/m^2$ in the spray, the coverage ratio of leguminous woody plants rather decreased compared to four treatment groups less than $3.8g/m^2$ seeding ratio. Based on the longitudinal data of coverage ratio in five treatment groups, we observed the gradual process of a short-term succession in which the dominant species shifted in the following order: First, cool-season turfgrasses; Second, herbaceous flowers; Third, leguminous woody plants. Comparing the appearance frequency of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria, Lespedeza cyrtobotrya appeared more frequent in 2014 whereas Indigofera pseudo-tinctoria appeared to be relatively more frequent in 2015. As a result, Indigofera pseudo-tinctoria was discovered to be a dominant species among woody plants. In this study, we observed that the optimal seeding quantity of Lespedeza cyrtobotrya and Indigofera pseudo-tinctoria was $2.4g/m^2{\sim}3.8g/m^2$. Moreover, the coverage ratio of 29.1%~35.4% and appearance frequency of 4.6plants~5.8plants were found in the condition of optimal seeding quantity.