• Title/Summary/Keyword: Diallel

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PEROXIDASE ACTIVITY ASSOCIATED WITH Puccinia sorghi INFECTION IN MAIZE (옥수수에 있어서 녹병감염에 관한 Peroxidase의 활성)

  • Kim Soon Kwon;Brewbaker James L.;Hasegawa Yoichi
    • Korean journal of applied entomology
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    • v.17 no.4 s.37
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    • pp.193-199
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    • 1978
  • Five parents and their 10 $F_1$ crosses of maize (Zea mays L.) were tested by means of diallel analysis for the inheritance of peroxidase activity following Puccinia sorghi rust infection. Peroxidase activity was measured at day zero and at 2 days and 8 days after inoculation. Peroxidase activity was increased significantly by P. sorghi infection in all 15 genotypes after 8 days, but not after 2 days. Highly significant differences in peroxidase activity were detected among the 15 genotypes. The highly genera] resistant inbred, CM105, and its hybrids showed exceptionally high peroxidase activity in both healthy and infected plants. However, another highly resistant inbred, Oh545, showed exceptionally low peroxidase activity. Significant general combining ability (GCA) and specific combining ability (SCA) means quares were detected for peroxidase activity independent of disease. GCA mean spuares, however, were consistently a major contiribution to the inheritance of peroxidase activity in the infected plants whereas SCA men square contributions were minor. Rust resistant maize plants controlling mongenic dominant $Rp_1^d$gene showed stronger peroxidase reroxidase responses than their susceptible counterparts in the gel electrophoresis and densiometric tracings. The increased peroxidase activity occurred in both major leaf peroxidases, $Px_3\;and\; Px_7$.

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Genetic Analysis of Thiobencarb Tolerance in Rice(Oryza sativa L.) (제초제(除草劑) Thiobencarb에 대한 벼 내성(耐性)의 유전(遺傳))

  • Shin, D.H.;Moody, K.;Zapata, F.J.;Kim, K.U.
    • Korean Journal of Weed Science
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    • v.10 no.3
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    • pp.179-185
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    • 1990
  • Five parents which showed differential response to thiobencarb and their $F_1$ hybrids were treated with thiobencarb (S-[(4-chlorophenyl) methyl]diethylcarbamothioate) at 2 and 4 kg ai/ha in order to investigate the inheritance of rice (Oryza sativa L.) to the herbicide. In the analysis of diallel crosses for tolerance of rice cultivars to the herbicide, highly significant GCA (general combining ability) and SCA (specific combining ability) effects were observed at both herbicide rates using the parameters of plant height and dry weight ; the GCA effect being greater than the SCA effect. The additive effects appeared to be more prevalent than dominance effects, and partial dominance was recognized in relation to herbicide tolerance in the different rice cultivars. Higher heritabilities in the narrow and broad senses were observed at 4 kg ai/ha. The heritabilities in the narrow sense for plant height and dry weight were 0.726 and 0. 743, respectively. IR9660-50-3-1 which showed the highest GCA effects seemed to possess more dominant genes related to herbicide tolerance than the other rice cultivars tested.

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Combining Ability Analysis and Selection Effectiveness for Tolerance to Cold-Induced Sterility in Rice (벼 장해형 내냉성의 조합능력검정과 선발효과)

  • Huhn Pal, Moon;J. Neil, Rutger
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.33 no.4
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    • pp.412-419
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    • 1988
  • Cold-induced sterility is a problem of rice (Oryza sativa L.) production in temperate regions and high elevation tropics. The present study was conducted to measure combining ability, determine character associations. and to evaluate effectiveness of F$_2$selection for tolerance to this type of cold injury. The F$_1$, F$_2$, F$_3$ and reciprocal backcross F$_1$ populations of a nine-parent diallel were grown at Davis, California. a relatively cool location for rice. Additive genetic variance was predominant but some non -additive genetic variance existed for cold tolerance. Good general combining ability (GCA) was generally associated with the cold tolerant parents, 'M-201', and 'L-201', and poor GCA with the most susceptible parent. 7703008. Sterility was positively correlated with late heading. negatively with mature plant height. and negatively with spikelet number. Of the three agronomic characters, heading date had the greatest influence on sterility. Selection of F$_2$'s in seven crosses involving M-101, the most cold-tolerant parent, was effective for increasing cold tolerance. Realized heritability estimates for cold tolerance averaged 0.53, and observed selection response for cold-induced sterility averaged -9.4 percentage points.

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Genetic Analysis of Low Temperature Germinability of Rice (벼의 저온발아성에 관한 유전분석)

  • Kang, Jong-Re;Ko, Mi-Suk;Kim, Ho-Young;Lim, Sang-Jong;Kim, Soon-Chul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.6
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    • pp.766-777
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    • 1997
  • The purpose of this study was to investigate inheritance modes of low temperature germinability(LTG) on rice. Two experiments were carried out: In experiment I, the seeds of 10 rice varieties yielded at the different cultivation conditions which were transplanted in a paddy field from May 20 to July 5 with 15-day interval, were tested at fixed temperature (13$^{\circ}C$) in-door and at field with natural conditions to find out the change of LTG according to different cultivating condition among the each variety, In experiment II, F$_1$ seeds of full diallal cross from six parents, were analyzed for combining ability and reciprocal differences of low temperature germinability. The results are summarized as follows. The seeds of 10 varieties showed significant differences of LTG at the different cultivation conditions, varieties and their interaction. And the relationship between LTG coefficient at 13$^{\circ}C$ and low temperature emergence(LTE) coefficient at field was highly significantly positive(r=0.511). From the analysis of the diallel cross, high significance was found at reciprocal difference and alpha inheritance. The mean squares of general combining ability(GCA) and specific combining ability of LTG were highly significant with higher mean squares of GCA. Seventeen out of 30 cross combinations showed transgressive segregation.

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Correlation and Combining Ability of Days to Germination, Flowering and Ripening in F1 Hybrids of Petunia hybrida (페튜니아 일대 잡종에서 발아, 개화 및 종자 등숙소요일수 관련형질의 상호관계 및 조합능력)

  • Song, C.Y;Park, S.C.;Park, N.B.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.3 no.1
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    • pp.94-101
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    • 2001
  • The correlation among days to germination, days to flower, days to seed ripening and number of seeds per a pod and their combining were studied in 15 F1s from the partial six-parent diallel cross in Petunia hybrida. The days to flowering, days to germination and seed number per a pod showed high positive correlation together, however germination percentage and number of seed showed negative correlation with days to ability(SCA) were highly significant for all the characters. The estimates of variance components of SCA were greater than those of GCA for all the characters, showing preponderance of non-additive gene actions for these characters. The line of A and C showed relatively high GCA effect for days to germination and days to flower and the lines of C and G for germination, days to seed ripening and number of seeds per a pod. The crosses of D×G and E×F exhibited high SCA effect for decreasing days to flower and days to germination, and increasing germination percentage and number of seeds per a pod. The broad sense heritability was generally high compared to narrow sense one in the 5 characters. The higher heritability values of both broad and narrow sense were shown in the days to ripening and number of seeds per a pod.

Studies on the Inheritance of Heading Date in Wheat(Triticum aestivum L. em Thell) (소맥(Triticum aestivum L. em Thell)의 출수기 유전에 관한 연구)

  • Chang-Hwan Cho
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.15
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    • pp.1-31
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    • 1974
  • Introducing genes for earliness of wheat varieties is important to develop early varieties in winter wheat. In oder to obtain basic informations on the response of heading to the different day length and temperature treatments and on the inheritance of heading dates, experiments were conducted at the field and greenhouse of the Crop Experiment Station, Suwon. Varieties used in this experiments were, early variety Yecora F70, medium varieties Suke #169, Parker and Yukseung #3, and late varieties Changkwang, Bezostaia, Sturdy and Blueboy. The parents and F$_1$s of partial diallel crosses of above eight varieties were subjected the following four different treatments; 1. high temperature and long day, 2. high temperature and short day, 3. low temperature and long day, and 4. low temperature and short day. The same materials were grown also in field condition. Parents, F$_1$ and F$_2$ generation were grown also in both greenhouse under high temperature and short day and in field. The results obtained were summarized as follow: 1. No effects of temperature and daylength on the number of leaves on the main stem were found when -varieties were vernalized. The number of main stem leaves were fewer for spring type of varieties than for winter type of varieties. 2. The effects of temperature and daylength on the days to flag leaf opening were dependent on the speed of leaf emergence. The speed of leaf emergence were faster for lower leaves than for upper leaves. 3. The response to short day and long day (earliness of narrow sense) of varieties were found to be direct factor responsible to physiology of heading dates in vernalized varieties. Great difference of varieties to heading date was found in high temperature and short day treatment, but less differences were found in high temperature and long day, low temperature and long day and low temperature and short day treatments respectively. The least varietal difference for heading dates was found in the field condition. 4. Changkwang and Parker were found to be the most sensitive to short day treatment (photosensitive) and the heading of these varieties were delayed by short day treatment. No great varietal differences were found among other varieties. 5. Varietal differences of heading dates due to daylength were greater in high temperature than in low temperature. 6. Varietal differences of heading dates due to temperature were not great. but in general the heading dates of varieties were faster under high temperature than under low temperature. 7. Earliness of heading dates was due to partial dominance effect of genes involved in any condition. The degree of dominance was greater under short day than under long day treatment. 8. The varietal differences of heading date under high temperature and long day were due to earliness or narrow sense (response to long day) of varieties. The degree of dominance was greater for Yecora F70, spring type than for other winter type of varieties. No differences or less differences of degree of dominance was found among winter type of varieties. The estimated number of effective factor concerned in the earliness of narrow sense was one pair of allele with minor genes. 9. The insensitivity of varieties to short day treatment in heading dates was due to single dominant gene effect. Under the low temperature the sensitivity of varieties to short day treatment was less apparent. 10. The earliness of short day and long day (earliness of narrow sense) sensitivities of varieties appearea to be due to partial dominance of earliness over lateness. In strict sense, the degree of the dominance should be distinguished. 11. Dominant gene effects were found for the thermo-sensitivity of varieties, and the effect was less, significant than the earliness in narrow sense. 12. One pair of allele, ee and EE, for photosensitivity was responsible for the difference in the heading dates between Changkwang and Suke #169. Two pairs of alleles, ee, enen and EE, EnEn. appeared to be responsible for the difference between Changkwang and Yecora F70. The effects of EE and EnEn were, additive to the earliness and the effects of EE were greater than EnEn under short day. However, the effects of EE were not evident in long day but the effects of EnEn were observed in long day. 13. Two pairs of dominant alleles for the earliness were estimated from the analysis of F$_1$ diallels in the field but the effects of these alleles in F$_2$ were not apparent due to low temperature and short day treatment in early part of growth and high temperature and long day treatment in later part of growth. The F$_2$ population shows continuous variation due to environmental effects and due to other minor gene effects. 14. The heritabilities for heading dates were ranged from 0.51 to 0.72, indicating that the selection in early generation might be effective. The extent of heritability for heading dates varied with environments; higher magnitude of heritability was obtained in short day treatment and high temperature compared with long day and low temperature treatments. The heritabilities of heading date due to response to short day were 0.86 in high temperature and 0.76 in low temperature. The heritabilities of heading date due to temperature were not significantly high. 15. The correlation coefficients of heading dates to the number of grains per spike, weight of 1, 000 grains. and grain yield were positive and high, indicating the difficulties of selections of high yielding lines from early population. But no significant correlation coefficient was obtained between the earliness and the number of spikes, indicating the effective selection for high tillering from early varieties for high yielding.

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Genetic Analysis for Traits at Birth and Weaning in Mice (Mouse의 생시(生時) 및 이유시(離乳時) 형질(形質)에 대(對)한 유전분석(遺傳分析))

  • Choi, Kwang Soo;Park, Hang Kyun;Hyun, Byung Hwa
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.158-165
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    • 1985
  • The study was conducted to obtain some genetic information for breeding of mice. Average performance, heterosis and genetic variance were estimated with 362 progenies from a full diallel cross of four lines of mice (BALB/c, CBA, C3H and C57BL). The progenies were reared at the Experimental Animal Farm. College of Agriculture, Kyungpook National University from November, 1984 to February, 1985. Data for litter size. sex ratio, body weight and weaning rate were analyzed into heterosis effects, and genetic variance with Hayman's model. The results obtained are summarized as follows : 1. Average performance was 7.54 in litter size, 53.20 % in sex ratio, 1.55 g in birth weight, 10.45 g in weaning weight and 94.13 % in weaning rate. 2. The estimated heterois was 6.97% in litter size, 7.26 % in sex ratio, 6.08% in birth weight, 3.54% in weaning weight and 2.05% in weaning rate, respectively. 3. Additive gene effects were not observed in litter size, sex ratio, birth weight, weaning weight and weaning rate. In litter size and weaning weight, dominance effects were observed, which were shown due to individual crosses in litter size and were shown mean dominance effects of parental line in weaning weight. Mat-Maternal effects appeared in birth weight and weaning weight, and reciprocal effects were observed in weaning weight.

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Breeding Hybrid Rice with Good Quality and High Yield II. Combining Ability of the Cytoplasmic-Genetic Male Sterile and Restorer Lines with Backgrounds of Japonica Rice, and Heterosis for Yield and Grain Quality of the Hybrid Rices (양질 다수성 일대잡종 벼 육성 연구 II. 새로 육성된 Japonica형 웅성불임 및 임성회복계통의 조합능력과 일대잡종 벼의 잡종강세 정도 및 미질)

  • Hak-Soo SUH
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.38 no.5
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    • pp.418-424
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    • 1993
  • In order to test combining ability of the cytoplasmic-genetic male sterile(CGMS) and restorer lines with backgrounds of Korean japonica rice varieties, diallel crosses were made among the early maturing CGMS lines Sobaegbyeo A, Odaebyeo A, Gwanagbyeo A and Daeseongbyeo A, and the restorer lines Sobaegbyeo R, Odaebyeo R, Gwanagbyeo Rand Daeseongbyeo R, and among the medium maturing CGMS lines Hwajinbyeo A, Paldal A, Suwon 224 A and Iri 386 A and the restorer lines Hwajinbyeo R, Paldal R, Suwon 224 Rand Iri 386 R, and among the late maturing CGMS lines Nagdongbyeo A, Palkweng A, Hwacheongbyeo A and Milyang 97 A and the restorer lines Nagdongbyeo R, Palkweng R, H wacheongbyeo Rand Milyang 97 R. The fourty eight combinations of the hybrids, their parents and the maintainers were grown in field condition in 1992. General combining ability, relative specific combining ability and relative combining ability for yield of the CGMS and restorer lines were tested. Combining ability of the CGMS lines Odaebyeo A, Iri 386 A, Nagdongbyeo A and Palkweng A, and of the restorer lines Odaebyeo R, Hwajinbyeo R, Hwacheongbyeo Rand Palkweng R was relatively high. Heterobeltiosis for grain yield over the better parent of the early maturing hybrids ranged from -17 % to 15%, and that of the medium maturing ones from -4% to 22%, and that of the late maturing ones from -46% to 30% respectively, Standard heterosis over the standard variety of the early maturing hybrids ranged from -13 % to 12 %, and that of the medium maturing ones from 0 % to 26%, and that of the late maturing ones from -38% to 26% respectively, Positive and negative heterosis for grain yield of the hybrid rices were observed depending on cross combinations, In the positive heterosis hybrids, number of panicles per hill and number of spikelets per panicle contributed mostly to grain yield, while in the negative heterosis hybrids, spikelet fertility and 1000 grain weight were closely correlated to grain yield. Amylose content of the hybrid rices, maintainer and restorer lines was mostly lower than 20 %, and alkali digestion value was higher than 6.0. Grain appearance of the hybrids tested was similar to the korean japonica rice varieties.

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Genetic Expression of Color Approved by Color Qualities of Munsell System on the Cocoon of Silkworm, Bombyx mori (누에 고치색 유전자 발현의 다양성 검정 및 색채과학적 해석)

  • Han, Myung-Sae;Park, Hyun-Ro;Han, Sang-Mi;Nam, Ki-Soo;Kwon, Soon-Ha;Lim, Jong-Sung;Nguyen, Mau Tuan
    • Journal of Sericultural and Entomological Science
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    • v.41 no.1
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    • pp.20-28
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    • 1999
  • Color qualities investigated on the basis of Munsell code and Korean standard color code for the cocoons from various strain of mulberry silkworm, Bombyx mori. 16 of different color qualities were identified from 21 of original silworm strains, and determinated international name with the revision of Korean color name for cocoon. The various cocoon color confirmed on the sphere from 567 to 593 nm wavelength, 78% of those located at the region about 580 nm (575~584) of sensitive "color difference limen". Y gene engaged broad ranges of wavelength (575~593 nm) in the color expression, by contrast with other genes of Pk (593 nm), F (584~593), Grc and relative G group (567~570 nm), on the transmission of carotenoid or flavonoid color substance. YC gene expression by original silk worm strain was also distinguished by those variation of specific yellow than other colors from Grc, GaGb, Gc, and YPkF. Appearance of chrome yellow cocoon was dominant than other yellow in the cross among vivid yellow group. F1 of pin${\times}$green produced the cocoon of yellow such as "additive mixture" as color light, however, most of the hybrid between yellow cocoon showed the color similar to "subtractive mixture" as a mixture of dyestuff. Hybrid cocoons among yellow or green colors were decreased their hue, value, and chroma, than those of parent. Diallel cross among the strain of various green cocoon suggest the existence of Grc, Ga, Gb, Gc genes. Cream colored cocoon of B. mandarina was differed from other yellow cocoon of Bobyx mori B. mori. Y$^A$ with Ymc showed the similar role of Y with C, therefore, segregated yellow cocoon from the B. mori${\times}$B. mandarina (+$^YC/Y^AYmc$). YC expression of $Y^AY$mc genes might be suppressed by deficiency of outer layer sericin on the middle division of silk giand in the B. mandarina.

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The Test of Combining Ability and Heterosis on the Silkworm(Bombyx mori) Breeding (누에 견.사질에 관한 잡종강세 및 조합능력검정)

  • 문병원;한경수
    • Journal of Sericultural and Entomological Science
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    • v.36 no.1
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    • pp.8-25
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    • 1994
  • The study was conducted to obtain the genetic information on heterosis and combining ability of the quantitative characters for F1 hybrid breeding in silkworms. Six parental varieties and each set of 30 diallel crosses in F1's were used as materials, and bred on the randomized complete block design with three replications. Fourteen characters were observed with the twenty samples in each tray. The data were analyzed for (1) heterosis and combining ability in F1 hybrid. The heterosis in the weight and the length of cocoon showed positively high at 24.51%, and 23.4%, respectively and the weight of the whole cocoon as well as the weight of the whole cocoon layer showed a siginificant heterosis ranging from 15.56% to 15.71% and from 17.14% to 19.01%, but the fifth and the total instar period showed negative heterosis. It was found that the combination between, C70XRomogua and N9 X Romogua showed highly a negative heterosis on the fifth instar period and for the cocoon weight. The female of N9+Sansuian and the male of Romogua X Sansurian have a high heterosis effect, on the cocoon shell weight, and Sansurian X Romogua(reciprocal) on the length and the weight of cocoon filament with no regard to sexuality. The significant maternal and cytoplasmic effect on heterosis of the cocoon weight and the cocoon shell weight were observed with the combinations, N9 X C5, N63 X C70 and on the length of the cocoon filament with the combinations, Sansurian X N63, Sansurian X C5, Sansurian X C70 and N9 X C70, N63 X C70 on the weight of cocoon filament. As mean squared of GCA, SCA and RCA were significant with these combining ability for all characters resulted from additive and non-additive altogether and there is a significant difference between reciprocals. Sansurian showed a negative GCA effect on the fifth and total larval duration, but the higher positive GCA effects took places with varieties N9 and C5 on the length, width, weight of cocoon, cocoon shell weight, percentage of cocoon shell weight, length and weight of cocoon filament, percentage of raw-silk with no regard to both generations and silkworm sexuality. The values of SCA between the cross combinations varied generation-wise and sex-wise. It was shown that SCA value for the fifth instar period was highly negative for Sansurian X C70, Romogua X C70, Sansurian X C5, Romogua X C5, but it was positive effect on the cocoon weight, cocoon shell weight with N9 X C5, and C70 X Sansurian, on the length of cocoon filament with N9 X C5, Romogua X Sansurian on the weight of cocoon filament between Romogua and N63 and on the percentage of raw-silk between the combination of Sansurian X Romoga.

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