• Reproductive and Developmental Biology
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• v.34 no.3
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• pp.181-184
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• 2010

Osmolarity of culture media is one of the most important factors affecting in vitro development. This study was conducted to investigate the DNA methylation status of Pre-1 and satellite sequence in pig nuclear transfer (pNT) embryos produced under different osmolarity culture conditions. Control group of pNT embryos was cultured in PZM-3 for six days. Other two treatment groups of pNT embryos were cultured in modified PZM-3 with 138 mM NaG or 0.05M sucrose (mPZM-3, 320 mOsmol) for two days, and then cultured in PZM-3 (270 mOsmol) for four days. Previous our studies have reported that pNT embryos cultured in both hypertonic media showed significantly higher blastocyst formation rate than that of control. The DNA methylation status of the satellite sequences in blastocyst was characterized using bisulfite-sequencing technology. The satellite region had a similar methylation pattern of in vivo blastocyst among two culture groups excepting the control group. Each level of methylation is that the satellite DNA moderately methylated (43.10% of PZM-3; 56.12% of NaCl; 55.06% of sucrose; 60.00% of in vivo embryos). As a result of the sequence of PRE-1, CpG methylation pattern was similar to three groups, including in vivo group. In case of the satellite DNA region, the osmolarity conditions were affected CpG DNA methylation status while PRE-1 sequence was not affected CpG DNA methylation in pNT blastocyst stage. These results indicate that the modification of osmolarity in a culture media may influence to spatially change of DNA methylation of repetitive sequence for pNT embryo development.

• Development and Reproduction
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• v.15 no.2
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• pp.121-131
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• 2011

The aim of this study was to investigate the egg development and the color change of Amphiprion melanopus, by the live food and thyroid hormone. After fertilizing, it took 168 h until hatching. The size of hatching larvae were $3.5{\pm}0.3$ mm, and some individuals had small yolk sac. As reaching the particular age, they underwent the color change (metamorphosis) - Days after hatching (DAH) 10: black, DAH 15~20: the 3 white stripes appear, DAH 30: the dorsal & the caudal white stripe begin to disappear. DAH 90: body color become red and orange, adult: cinnamon body color and a white stripe on head. - The group fed enriched Artemia with Tigriopus japonicus grew the faster than with only Artemia. The fin coloration of the one fed the only T. japonicus has a vivid red and orange color. $T_3$-treated group (4 ppm) grew the faster than the others (0, 2, 6 ppm). The size of the 6 ppm group was the smallest. The results suggest T. japonicus can be used as a supplement with rotifer and Artemia for the juvenile development and coloration. $T_3$ is considered to be involved in the metamorphosis and color formation of Amphipirion sp.

• Reproductive and Developmental Biology
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• v.35 no.3
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• pp.281-285
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• 2011

In the present study, we identified differentially methylated region (DMR) upstream of Dnmt1o and Dnmt1s gene in early porcine embryos. Porcine Dnmt1o had at least one DMR which was located between -530 bp to -30 bp upstream from transcription start site of the Dnmt1o gene. DNA methylation analyses of Dnmt1o revealed the DMR to be hypomethylated in oocytes, whereas it was highly methylated in sperm. Moreover, the DMR upstream of Dnmt1o was gradually hypermethylated from oocytes to two cells and dramatically changed in the methylation pattern from four cells to BL stages in an in vivo. In an IVF, the methylation status in the DMR upstream of Dnmt1o was hypermethylated from one cell to eight cells, but demethylated at the Morula and BL stages, indicating that the DNA methylation pattern in the Dnmt1o upstream ultimately changed from stage to stage before the implantation. Next, to elucidate whether DNA methylation status of Dnmt1s upstream is stage-by-stage changed in during porcine early development, we analyzed the dynamics of the DNA methylation status of the Dnmt1s locus in germ cell, or one cell to BL cells. The Dnmt1s upstream was highly methylated in one and eight cells, while less methylated in two, four, morula, and BL cells. Taken together, our data demonstrated that DNA methylation and demethylation events in upstream of Dnmt1o/Dnmt1s during early porcine embryos dramatically occurred, and this change may contribute to the maintenance of genomewide DNA methylation in early embryonic development.

• Journal of Korean Academy of Nursing Administration
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• v.5 no.1
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• pp.5-21
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• 1999

Nursing organization culture is common patterns of believing, thinking and behaving resulted from a variety of experiences and interaction nurses in the same setting. This study was performed based on the assumption of existence of different subcultures within meta-culture, to identify the differences of subculture among 5 nursing units and to analyze the relationship between nursing organization culture and organizational commitment In this study, two kinds of instruments were used. One was the instrument of nursing organization culture developed by researcher through literature review and interview with nurses. The other one was Mowday's Organizational Commitment Questionnaire to measure organizational commitment of nurses. Both of them were structured with 5 scale. The answers were analyzed using SPSS program. The results were as follows : The meta-culture of the nursing organization culture was the people stable culture. There were significant differences in people development culture and people stable culture among 5 nursing units and all 5 units had different culture score. Especially, emergency room had strong development culture, and stable culture was dominant in operating room. Other units except emergency room and operating room had high scores in people stable culture than other culture types, but revealed different distribution. There were significant differences of the nursing organizational culture types -people development, task development, people stable, task stable-among 5 units. Four types of nursing organizational culture consisted of competing values in one organization. Nurses's organizational commitments were sign ificantly different among the units. The score per ceived by nurses who work in emergency room, operating room and leu was higher commitment score than that of medical/surgical nursing units Nurse's commitment to nursing organization was also related to total work period as nurse in any setting and work period in this hospital. Organizational commitment was significantly different among the nursing culture types, indicating that the scores of developmental culture were higher than stable culture. In conclusion, there were many different subcultures in nursing organization. In subculture, the organizational commitment was different. Therefore, the change of nursing organization culture or nursing unit culture needs to be considered to hire, give orientation, teach. and reallocate nurses efficiently. Research on nursing organization culture using both qualitative and quantitative method needs to be further considered. Furthermore, the strategy in nursing organization culture for nursing administrator to manage human resources efficiently and to change nursing unit effectively, needs to be developed.

• Journal of the Korean Society of Costume
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• v.6
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• pp.7-26
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• 1982

Investigation for industrial revolution has been recognized as an important issue of historical science, since industrial revolution itself was a historical moment in modern economic society and in the forming of modern capitalistic culture. If clothing culture had been developed in close relation to social culture. industrial revolution which gave a base for modern capitalistic culture, would be a birth of modern clothing as well as a moment for modernization of western clothing. As it takes for granted that industrial revolution, historical phenomenon is a developmental base of modern clothing culture. This study tried to enlighten the origin of modern clothing culture phenomenon with investigation of industrial revolution as a historical moment in modern clothing culture through documental study. Historian's point of view and method of studying are important when we investigate the clothing phenomenon. Although culture phenomenon of industrial revolution has been evaluated usually through socialogical aspect, studying for clothing culture phenomenon must be carried out on aethetical as well as well as sociological aspect, on account of dualism of clothing, as it is social and artistic nature. In 19th century, there were technical improvement, changing patterns of production, changing social relation and beginning of mass culture. At the same time clothing culture phenomenon was changed in relation to modern capitalistic society. The findings of the study could be summarized as follows. 1. Technical improvement in textile industry and fitting process brought about mechanization of clothing industry. 2. Appearance of popular clothing culture made it easy to spread to various classes of society. 3. Development of transportation system and communication channel made it internationalization of western clothing. 4. Recognition of importance in functional style of clothing. 5. The pursuit of modern aesthetics made rapid changing mode of clothing. Above distinctive features of modern clothing culture were derived from by-product of machinary culture, mass culture, internationalism, rapidly changing mode of various culture during industrial revolution. Industrial revolution was a change of the material world as well as in industry owing to machine advent, then the culture part that displayed directly these changes was the plastic arts of living that mould the material. The problem of clothing construction caused by industrial revolution was solved by pursuing the functional aesthetics. Clothing phenomenon as a process of value transfer participates mass culture in closs relation to general change of various culture caused by industrial revolution. Therefore western clothing gained the qualities of modern culture, condensed as function, and popularity in the process of modernization.

• 한국사회복지학회:학술대회논문집
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• 2000.04a
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• pp.615-628
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• 2000

It is said that stress is the threat of peace and excess of resources which man has from special relationship between individual and environment. This study is for suggesting the method of intervention of the clinical social work service concerning stress for the adolescents who can conceive looking into the relationship between adolescent pregnancy and stress through the sundry records for them. When the change of making into parents from pregnancy overlapped with stress in the cycle of life as an adolescent period itself, the accumulated stress grows much bigger. The repeated change causes an obscurity of individual role and worsens stress and brings confusion on a priority ranking of developmental task. The pregnancy of adolescence has a demoralizing influence upon the adolescents themselves who are going to be adults, their children, and society. These negative effects won't be end in a short period so the effort of intervention in necessary to improve the coping skills and adaptability of pregnant adolescents. In case of pregnant adolescents, the approach of personal counsel is not just enough and effective prevention program can be better alternative and purpose at the same time. This intervention protects adolescents from the distress which can be easily disclosed after delivery and contribute to promote a good mental health of adolescents who will grow to be adults later. After all it implies the necessity of stress management program which can improve the coping ability against stress and develop positive recognition for the pregnant adolescents. Therefore much profounder study for practical intervention program, implementation, and evaluation is necessary with the conclusion which has the results of this literature study.

• The Korean Journal of Malacology
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• v.28 no.2
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• pp.175-185
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• 2012

To clarify reproductive ecology of the melania snail Semisulcospira coreana (v. Martens, 1886) in Bukhan River, gonad development, fatness, gonad index, sex ratio, first sexual maturity of population, monthly change of larvae number and developmental stages in brood pouches were investigated by six identification methods. As maturation progrsses, the sex of the snali can be distinguishable easily by color:: the ovary being blue-green and testis light yellow. The sex ratio of female to male individuals over 13.95 mm shell height was significantly different from 1:1 (${\chi}^2$ = 38.45, p < 0.05). The sex ratio of female to male individuals changed drastically according to the season, Based on the monthly variations of fatness, gonad index and histological analysis, spawning occurred twice a year (spring and autumn) and the mean size of matured eggs was $450{\mu}m$ in diameter. The monthly change of larval number in brood pouch showed also two distinct peaks in March and September during the year. The average number of larvae in brood pouches was 286 - 862 individuals. In this study, the number of larvae in the brood pouches were a minimum in December and a maximum in March (975 larvae). The biological minimum size (the size at 50% of group sexual maturity) of the melania snail was 13.95 mm in shell height in females and males. All females over 15 mm in shell height possesed brood pouches.

• Korean Journal of Environment and Ecology
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• v.29 no.6
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• pp.858-864
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• 2015

Developmental changes in the vocal signals of serotine bats (Eptesicus serotinus) during their infancy were examined in this study. The analysis was conducted on 4 infant serotine bats from 1 to 40 days after their birth. Pulse duration (PD), pulse interval (PI), peak frequency (PF), maximum frequency ($F_{MAX}$), minimum frequency ($F_{MIN}$), and bandwidth (BW) were measured. As the bats grew, their vocalizations became increasingly consistent and similar to those of adults. For infant bats, PD and PI decreased as they grew older, whereas PF, $F_{MAX}$, $F_{MIN}$, and BW increased. The greatest change in vocalizations was observed between the $10^{th}$ and $20^{th}$ days after birth. Also, PF, $F_{MAX}$, $F_{MIN}$ and BW, which describe sound frequency, increased dramatically during the period between the $10^{th}$ and the $20^{th}$ days. In contrast, the greatest change in PD occurred between the $30^{th}$ and $40^{th}$ days after birth. The results collected in this study suggest that frequency increased as the contraction ability of the muscles developed by around 20 days of age. Muscle relaxation ability, which is related to PD, was found to develop significantly at 30 to 40 days of age. According to the results of this study, although 40 day-old infant bats are not yet able to fly, their vocal signals were similar to those of adults. This indicates that vocal development and flying activity develop separately in young bats.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.3
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• pp.187-195
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• 2019

The needs for precise diagnostics and farm management-decision aids have increased to reduce the risk of climate change and environmental stress. Crop simulation models have been widely used to search optimal solutions for effective cultural practices. However, limited knowledge on physiological responses to environmental variation would make it challenging to apply crop simulation models to a wide range of studies. Advanced research facilities would help investigation of plant response to the environment. In the present study, the sunlit controlled environment chambers, known as Korean SPAR (Soil-Plant-Atmosphere-Research) system, was developed by renovating existing SPAR system. The Korean SPAR system controls and monitors major environmental variables including atmospheric carbon dioxide concentration, temperature and soil moisture. Furthermore, plants are allowed to grow under natural sunlight. Key physiological and physical data such as canopy photosynthesis and respiration, canopy water and nutrient use over the whole growth period are also collected automatically. As a case study, it was shown that the Korean SPAR system would be useful for collection of data needed for understanding the growth and developmental processes of a crop, e.g., soybean. In addition, we have demonstrated that the canopy photosynthetic data of the Korean SPAR indicate the precise representation of physiological responses to environment variation. As a result, physical and physiological data obtained from the Korean SPAR are expected to be useful for development of an advanced crop simulation model minimizing errors and confounding factors that usually occur in field experiments.

• Korean Journal of School Psychology
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• v.11 no.1
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• pp.253-274
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• 2014

The present study classified groups based on latent profile of self-determination motivation(amotivation, external motivation, intrinsic motivation), and examined the determinants for each group. The data was collected through panel data of Korea Education Longitudinal Study(KELS), total 5,459 participants who answered questionnaires of self-determination motivation of two times both second grade of middle school and second grade of high school. To identify the change motivational type, standardized residual was conducted using SPSS 17.0., and the latent classes for the change of motivational type was investigated using M-Plus in the frame work of Latent Profile Analysis(LPA). The results indicated that five groups(increase of self-determination, self-determination maintenance, self-determination developmental delay, elf-determination confusion, decrease of self-determination group) were classified based on latent profile. In addition, parental control, academic self-concept, teacher-student relationship, test anxiety, avoidance orientation, gender, father's education, and income were significantly related to each group. Lastly, the implications for directions of the adolescent counseling, limitations and future research are discussed.