• Title/Summary/Keyword: Developmental biology

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An Immunological Approach to ABA Receptor and its Gene

  • Xie Zhou;Jin, Zhen-hua;Zheng, Zhi-fu;Kai Xia;Zhang, Neng-gang;Wan, Yin-sheng;Sang, Yong-ming;Chen, Kao-shan;Liu, Shi-ming
    • Proceedings of the Botanical Society of Korea Conference
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    • 1996.06a
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    • pp.68-78
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    • 1996

  • Two types of immunoloigcal probes, anti-ABBP Abs, have been developed. The purified ABBP from ABA-C1-BSA-sepharose 4B column was identified by PAGE and appeared in one band of about 56KD, as well as showed a specific binding ability and a high affinity for ABA (Kd2.0$\times$10-9 mol/L). Unexpectedly, the existence of rRNA with a length of around 300 nucleotides could be found, when the ABBP was digested with proteinase K and identified by eletrophorsis on an agarose gel (1%). As a result, about 120 cDNA clones coding maize 17s RNA and only one cDNA clone coding ABBP (24cDNA) were obtained from 200,000 seperated phage plaques by the anti-ABBP pAbs. 24cDNA had 1075bp and contained an open reading frame coding 254 amino acids. The anti-idiotypic Ab raised against an ABA MAb showed the ability of either mimicking ABA or competing with ABA. The localization of ABBPs in plant cell was investigated.

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Bovine Follicular fluid and Serum Share a Unique Isoform of Matrix Metalloproteinase-2 That Is Degraded by the Oviductal Fluid

  • Kim, Minjung;Minjeong Hong;Kim, Jisoo;Kim, Haekwon;Lee, Seung-Jae;Kang, Sung-Goo;Cho, Dong-Jae
    • Proceedings of the Korean Society of Developmental Biology Conference
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    • 2001.02a
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    • pp.55-56
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    • 2001

  • Gelatin zymograms of bFF and bS showed GA110 and 62 kDa gelatinses in adsition to several minor ones. Of these, GA110 gelatinase was abolished by treating bFF or bS with bOF and interestingly, its enzymatic activity was enhanced by adding EDTA to bFF or bS before zymographic analyses. Experiments using specific inhibitors of MMPs indicated that GA110 and 62 kDa proteins were indeed gelatinases. Immunoblotting experiments using an antibody against human MMP-2 showed that both GA110 and 62 kDa were an MMP-2 isoform and active MMP-2, respectively. The results suggest that the interaction between bFF and bOF can occur at the time of fertilization.

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