• Journal of the Korean Society for Precision Engineering
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• v.21 no.9
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• pp.111-118
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• 2004

The automatic assembly system of micro optical filter is a key technology in the development of optical modules with high functionality. In order to develop an automatic assembly system of optical fiber and filter, we have firstly developed the system and program capable of characterization of 30${\mu}m$-thick film filters as well as conventional optical filters. Moreover, we have carried out the characterization of optical filter using the developed system and program, and compared experimental results with by conventional handwork. The measurement of optical filters using the present system is faster, more precise and more reliable than those based on the conventional handwork.

• Proceedings of the Korean Nuclear Society Conference
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• 2005.10a
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• pp.268-269
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• 2005

• Proceedings of the Materials Research Society of Korea Conference
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• 1998.08a
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• pp.98.4-98
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• 1998

• Proceedings of the Korean Society of Crop Science Conference
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• 2020.06a
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• pp.140-140
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• 2020

• Proceedings of the PSK Conference
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• 2000.04a
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• pp.221.1-221.1
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• 2000

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2002.11a
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• pp.81.1-81
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• 2002

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2003.10a
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• pp.85-85
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• 2003

• Proceedings of the Korean Society of Life Science Conference
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• 2007.10a
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• pp.78.1-78.1
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• 2007

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• Journal of Animal Reproduction and Biotechnology
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• v.38 no.1
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• pp.26-31
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• 2023

Chicken embryonic stem (ES) cells have great potential and provide a powerful tool to investigate embryonic development and to manipulate genetic modification in a genome. However, very limited studies are available on the functional characterization and robust expansion of chicken ES cells compared to other species. Here, we have developed a method to generate chicken embryonic stem cell-like cells under pluripotent culture conditions. The chicken embryonic stem cell-like cells were cultivated long-term over several passages of culture without loss of pluripotency in vitro and had the specific expression of key stem cell markers. Furthermore, they showed severe changes in morphology and a significant reduction in pluripotent genes after siRNA-mediated NANOG knockdown. Collectively, these results demonstrate the efficient generation of chicken embryonic stem cell-like cells from EGK stage X blastoderm-derived singularized cells and will facilitate their potential use for various purposes, such as biobanking genetic materials and understanding stemness in the fields of animal biotechnology.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.20-20
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• 2003

We described the isolation, characterization and inheritance of twenty-seven microsatellite loci from olive flounder, Paralichthys olivaceus. All loci were found to be polymorphic, and had between five and 22 alleles with observed heterozygosity ranging from 0.161 to 1.0 in 31 individuals examined. Allele segregation patterns of all loci in controlled crosses of flounder were studied to assess the inheritability. Allele of all but 3 loci were segregated according to Mendelian transmission. However, 3 loci had a possibility of scoring errors of heterozygous individuals caused by unreproducible PCR amplification of a particular allele. 24 microsatellite loci are likely to be useful for studies of genome mapping, mating systems and population genetics in this species.