• The Korean Journal of Zoology
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• v.38 no.3
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• pp.348-355
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• 1995

This Investigation was carried out to map the morphological development of serotonin-immunoreactive neurons in the larval brain of the cabbage butterfly, Artogeia rapae, during five larval stages. Both the first instar larva and the second instar larva contained twenty serotonin-immunoreactive (5-HTi) neurons in each brain. The fibres of 5-HTI commissure was interconnected to two cerebral hemispheres in both brains. However, the 5-HTi commissural fibres was Increased in number in the second-instar larva brain. In the brain of the second Insar larva these 5-HTi fibres formed rich arborization in contralateral neuropils, especially In the posterior parts of it. The third-Instar larva braIn, which Included twenty two 5-HTi neurons, had three groups of 5-HTi commissural fibres. In the fourth Instar larva, the number of 5-HTi fibres as well as 5-HTi cell bodies increased in the brain. The fifth-instar larva brain, which contained fifty four 5-HTi cell bodies, showed the largest number of 5-HTi cell bodies In developing larval brains. The 5-HTi fibres formed richest commissural fibres and some of them run parallel to anteroposterior axis.

• Animal cells and systems
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• v.5 no.3
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• pp.211-216
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• 2001

Polyclonal antiserum against Manduca sexta allatotropin has been utilized to investigate the localization of allatotropin-immunoreactivity in the brain of the si1k moth Bombyx mori. Manduca sexta allatotropin-immunoreactive (Mas-AT-IR) neurons were found in all larval brains investigated, but not in prepupal, pupal and adult brains. In the larval stages, first appearance of Mas-AT-immunoreactivity w8s shown in the brain of first instar larvae, which contains four pairs of bilateral Mas-AT-IR cell bodies. Labeled neurons increased to six pairs in the second instar larval brain, including two pairs of median neurosecretory cells in the pars intercerebralis. In the third and fourth instar larvae, five pairs of labeled cell bodies were distributed throughout each brain. In the fifth instar, there were about ten pairs of bilateral cell bodies in the day-1 brain, about seven pairs in the day-3 brains, and five pairs in the day-5 brains, respectively. Mas-AT-labeling was observed in both axons within nervi corpora cavdiaci (NCC) 1+11 and corpora allata. This suggests that the Mas-AT produced from the brain neurons is transported via some axons of the NCC 1+11 and nervi corpora allati I to the corpora allata, which appears to be a main accumulation site for the Mas-AT neuropeptide in some brain neurons produced in B. mori.

• Proceedings of the Zoological Society Korea Conference
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• 1994.10a
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• pp.164.2-164
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• 1994

No Abstract, See Full Text

• Animal cells and systems
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• v.1 no.3
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• pp.475-482
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• 1997

The antiserum against locustatachykinin I, originally isolated from brain and retrocerebral complex of the locust Locusta migratoria, has been used to investigate changes in number, localization, and structure of locustatachykinin I-immunoreactive (LomTK I-IR) neurons in the brains of the common cutworm, Spodoptera Iitura, during postembryonic development. These neurons are found at larval, pupal, and adult stages. In the larval stages, the first instar larva shows the first appearance of about 8 LomTK I-IR neurons. These neurons gradually increase in number from the second to fourth instar larvae which have the largest number of about 92 in all postembryonic stages. Thereafter, these neurons decrease to about 28 in number in the 5-day-old pupa. However, they begin to rise again from the 7-day-old pupal stage, eventually reaching to about 90 in the l-day-old adult. The developing larval brains contain cell bodies of these neurons in most neuromeres. After the metamorphosis of larva to pupa and adult, localization of these neuronal cell bodies is confined to the specific cerebral neuromeres. The 7-day-old pupal brain shows the location of these neuronal cell bodies in pars intercerebralis, pars lateralis of protocerebrum, deutocerebrum, tritocerebrum, optic lobe-near region, and subesophageal ganglion. In the l-day-old adult, however, the brain has these cell bodies only in some neuromeres of protocerebrum, deutocerebrum, and subesophageal ganglion. Throughout the postembryonic life, changes in structure of these neurons coincide with changes in number and localization of these neurons. These findings suggest that changes in number, localization, and structure of these neurons reflect differentiation of these neurons to adult type.